Sequence of pCFJ70

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Sequence of pCFJ70

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Sequence of pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr : 7155 BP; 2076 A; 1480 C; 1471 G; 2128 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 60

1 aatatgtatc aactattaag tgaccggcag caaaatgttg cagcactgac ccttttggga 60

.......... .......... M13_f20 lacZ_a M13_pUC_f ..........

.......... .......... .......... .......... .......... ..........

61 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 120

61 ccgcaatggg ttgaattagc ggaacgtcgt gtagggggaa agcggtcgac cgcattatcg 120

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 180

121 cttctccggg cgtggctagc gggaagggtt gtcaacgcgt cggacttacc gcttaccgcg 180

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 240

181 gactacgcca taaaagagga atgcgtagac acgccataaa gtgtggcgta taccacgtga 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 300

241 gagtcatgtt agacgagact acggcgtatc aattcggtcg gggctgtggg cggttgtggg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 360

301 cgactgcgcg ggactgcccg aacagacgag ggccgtaggc gaatgtctgt tcgacactgg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga 420

361 cagaggccct cgacgtacac agtctccaaa agtggcagta gtggctttgc gcgctctgct 420

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 480

421 ttcccggagc actatgcgga taaaaatatc caattacagt actattatta ccaaagaatc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 540

481 tgcagtccac cgtgaaaagc ccctttacac gcgccttggg gataaacaaa taaaaagatt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 600

541 tatgtaagtt tatacatagg cgagtactct gttattggga ctatttacga agttattata 600

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 660

601 actttttcct tctcatactc ataagttgta aaggcacagc gggaataagg gaaaaaacgc 660

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

661 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 720

661 cgtaaaacgg aaggacaaaa acgagtgggt ctttgcgacc actttcattt tctacgactt 720

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 780

721 ctagtcaacc cacgtgctca cccaatgtag cttgacctag agttgtcgcc attctaggaa 780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 840

781 ctctcaaaag cggggcttct tgcaaaaggt tactactcgt gaaaatttca agacgataca 840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 900

841 ccgcgccata atagggcata actgcggccc gttctcgttg agccagcggc gtatgtgata 900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 960

901 agagtcttac tgaaccaact catgagtggt cagtgtcttt tcgtagaatg cctaccgtac 960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 1020

961 tgtcattctc ttaatacgtc acgacggtat tggtactcac tattgtgacg ccggttgaat 1020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 1080

1021 gaagactgtt gctagcctcc tggcttcctc gattggcgaa aaaacgtgtt gtacccccta 1080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 1140

1081 gtacattgag cggaactagc aacccttggc ctcgacttac ttcggtatgg tttgctgctc 1140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 1200

1141 gcactgtggt gctacggaca tcgttaccgt tgttgcaacg cgtttgataa ttgaccgctt 1200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 1260

1201 gatgaatgag atcgaagggc cgttgttaat tatctgacct acctccgcct atttcaacgt 1260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 1320

1261 cctggtgaag acgcgagccg ggaaggccga ccgaccaaat aacgactatt tagacctcgg 1320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 1380

1321 ccactcgcac ccagagcgcc atagtaacgt cgtgaccccg gtctaccatt cgggagggca 1380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 1440

1381 tagcatcaat agatgtgctg cccctcagtc cgttgatacc tacttgcttt atctgtctag 1440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1500

1441 cgactctatc cacggagtga ctaattcgta accattgaca gtctggttca aatgagtata 1500

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1560

1501 tatgaaatct aactaaattt tgaagtaaaa attaaatttt cctagatcca cttctaggaa 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1620

1561 aaactattag agtactggtt ttagggaatt gcactcaaaa gcaaggtgac tcgcagtctg 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1680

1621 gggcatcttt tctagtttcc tagaagaact ctaggaaaaa aagacgcgca ttagacgacg 1680

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

1681 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1740

1681 aacgtttgtt tttttggtgg cgatggtcgc caccaaacaa acggcctagt tctcgatggt 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 1800

1741 tgagaaaaag gcttccattg accgaagtcg tctcgcgtct atggtttatg acaggaagat 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1860

1801 cacatcggca tcaatccggt ggtgaagttc ttgagacatc gtggcggatg tatggagcga 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1920

1861 gacgattagg acaatggtca ccgacgacgg tcaccgctat tcagcacaga atggcccaac 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1980

1921 ctgagttctg ctatcaatgg cctattccgc gtcgccagcc cgacttgccc cccaagcacg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 2040

1981 tgtgtcgggt cgaacctcgc ttgctggatg tggcttgact ctatggatgt cgcactcgat 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 2100

2041 actctttcgc ggtgcgaagg gcttccctct ttccgcctgt ccataggcca ttcgccgtcc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 2160

2101 cagccttgtc ctctcgcgtg ctccctcgaa ggtccccctt tgcggaccat agaaatatca 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2220

2161 ggacagccca aagcggtgga gactgaactc gcagctaaaa acactacgag cagtcccccc 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg 2280

2221 gcctcggata cctttttgcg gtcgttgcgc cggaaaaatg ccaaggacca gaaaacgacc 2280

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 2340

2281 ggaaaacgag tgtacaagaa aggacgcaat aggggactaa gacacctatt ggcataatgg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcctttgagt gagctgatac cagcggataa caatttcaca caggaaacag ctatgaccat 2400

2341 cggaaactca ctcgactatg gtcgcctatt gttaaagtgt gtcctttgtc gatactggta 2400

.......... .......... .......... M13_pUC_r .......... M13_r

.......... .......... .......... .......... .......... ..........

2401 gattacgcca agctatcaac tttgtataga aaagttgagt gattatagtc tctgttttcg 2460

2401 ctaatgcggt tcgatagttg aaacatatct tttcaactca ctaatatcag agacaaaagc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttaattttga attttgcttg ataaggctgc aacaaagatc aggttgacat atattttcag 2520

2461 aattaaaact taaaacgaac tattccgacg ttgtttctag tccaactgta tataaaagtc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 taatttattt taacctgtac tctatcactg ccggctataa taagttcttg aataaaataa 2580

2521 attaaataaa attggacatg agatagtgac ggccgatatt attcaagaac ttattttatt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttttcccgac aaaacatgag tatttctttc gaaaataaaa gtgcaggcta attagagatt 2640

2581 aaaagggctg ttttgtactc ataaagaaag cttttatttt cacgtccgat taatctctaa 2640

.......... .......... .......... .......... .......... ..........

.......... .......... PmlI(2669) .......... .......... ..........

2641 attctgtaat taactgcata atttgtcacg tgccatagtt ttacattcca ctacgtcata 2700

2641 taagacatta attgacgtat taaacagtgc acggtatcaa aatgtaaggt gatgcagtat 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gttcttaaaa tactaatctc ctgaaaatag aagtaggtga agaaagttta attatcagtt 2760

2701 caagaatttt atgattagag gacttttatc ttcatccact tctttcaaat taatagtcaa 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctaaaatgac aattgatctt tggaatatgt tctgaaacta ccgatcattg aacagatgct 2820

2761 gattttactg ttaactagaa accttataca agactttgat ggctagtaac ttgtctacga 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atttgaatga tatagaattg tatatttgca atttctgaaa cgcgttctta aaggcacaca 2880

2821 taaacttact atatcttaac atataaacgt taaagacttt gcgcaagaat ttccgtgtgt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gattaattca aaagggtctg gccgcaaaaa ggtttatggt ggccgatttt gagttttgtg 2940

2881 ctaattaagt tttcccagac cggcgttttt ccaaatacca ccggctaaaa ctcaaaacac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... BglII(2989) .........

2941 tgtgattgct ttttcacaat cagtgttttc aggattatgt gatgaactag atcttcaagt 3000

2941 acactaacga aaaagtgtta gtcacaaaag tcctaataca ctacttgatc tagaagttca 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttcgttacat ttcatatgtt ttcggaactc acgaagtaca tattgggtat tgtgctcaaa 3060

3001 aagcaatgta aagtatacaa aagccttgag tgcttcatgt ataacccata acacgagttt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(3115)

3061 aaattcagca atcagcttcg ctccgctgac tttagaaccc aaaaaaatag tatggccaaa 3120

3061 tttaagtcgt tagtcgaagc gaggcgactg aaatcttggg tttttttatc ataccggttt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctgactgtgt tacgatcatt tcaatttttc aatacatatt taagatttct aagagtaaga 3180

3121 gactgacaca atgctagtaa agttaaaaag ttatgtataa attctaaaga ttctcattct 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aggtcaaaaa ctgttctgga atacatatat atttttcagg ttacaattag tcaaaaagtg 3240

3181 tccagttttt gacaagacct tatgtatata taaaaagtcc aatgttaatc agtttttcac 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cactgaaata tacgttttaa tttcacgaat aacccaatta gttcaatgta tttttggtca 3300

3241 gtgactttat atgcaaaatt aaagtgctta ttgggttaat caagttacat aaaaaccagt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... BclI(3340) .......... ..........

3301 accaacgtta aagtttggct tccaaccaat tatcatttct gatcaaccac aatgtttttt 3360

3301 tggttgcaat ttcaaaccga aggttggtta atagtaaaga ctagttggtg ttacaaaaaa 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctttatctgc aagttaattt tttattttta tccagatgtt tggcatattt ttcaattctt 3420

3361 gaaatagacg ttcaattaaa aaataaaaat aggtctacaa accgtataaa aagttaagaa 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cactagcgcc cacttcttgc acttccggcg ccctgaatct aatgcatctg ttgcaagaat 3480

3421 gtgatcgcgg gtgaagaacg tgaaggccgc gggacttaga ttacgtagac aacgttctta 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgaaagacca atcaacacat tgttttcttc acgagatact gaagaaaatg aataaaaaca 3540

3481 actttctggt tagttgtgta acaaaagaag tgctctatga cttcttttac ttatttttgt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gagaaaaaga gccatgtgat tagtgacaac tgttgctaac agataccata gcttggactt 3600

3541 ctctttttct cggtacacta atcactgttg acaacgattg tctatggtat cgaacctgaa 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggtacgtgat ggcaacgtat gggtcaacaa aaatgattgc agagggggtg caaaacagtc 3660

3601 ccatgcacta ccgttgcata cccagttgtt tttactaacg tctcccccac gttttgtcag 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aagtcgagaa aatatgaaaa acagaaaaca aagaacagaa aaatgggttt gagagtcagt 3720

3661 ttcagctctt ttatactttt tgtcttttgt ttcttgtctt tttacccaaa ctctcagtca 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ataatttata aaagaaaaat tgtacataga aattaaccat ttttgtagaa gaagttattt 3780

3721 tattaaatat tttcttttta acatgtatct ttaattggta aaaacatctt cttcaataaa 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ttcaagcatc gttaaaaatt attcaaagca ccttatttca tatttaattt taaacatggt 3840

3781 aagttcgtag caatttttaa taagtttcgt ggaataaagt ataaattaaa atttgtacca 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 taaatgaaca acacggtgcg caatcaggaa aacttgaaat ctgaaactgt tgttgtgatc 3900

3841 atttacttgt tgtgccacgc gttagtcctt ttgaacttta gactttgaca acaacactag 3900

.......... .......... .......... .......... .......... ..........

.......... .......... SpeI(3924) .......... .......... ..........

3901 ttcttcgcaa ctgttcagat agcactagtg taatgttaag agtgcgcgaa tataatggaa 3960

3901 aagaagcgtt gacaagtcta tcgtgatcac attacaattc tcacgcgctt atattacctt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tataatggat cacacctcct gccatcaggt aaacgtctct gttatcacat atttccaact 4020

3961 atattaccta gtgtggagga cggtagtcca tttgcagaga caatagtgta taaaggttga 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 attaaatttt taccttttac agttttacat ttttttgaaa aaagtaactt tttgtcttca 4080

4021 taatttaaaa atggaaaatg tcaaaatgta aaaaaacttt tttcattgaa aaacagaagt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... PstI(4122) ..........

4081 aaatccctga cgaaaatatc aaatatttta atcgagactg cagaggaacc gattgatgat 4140

4081 tttagggact gcttttatag tttataaaat tagctctgac gtctccttgg ctaactacta 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttggaaaatc cagctttacc tgtgtaagaa ctgaaaagtt tcataaccct agggtattcc 4200

4141 aaccttttag gtcgaaatgg acacattctt gacttttcaa agtattggga tcccataagg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagttacatt ccccactggc taacaatagc acccagtttt tcatcacctt tcttcaaatt 4260

4201 gtcaatgtaa ggggtgaccg attgttatcg tgggtcaaaa agtagtggaa agaagtttaa 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tctcggcgat ttgttaaaaa caaaatttgt gtcccttctc tgatatctct atgtctctaa 4320

4261 agagccgcta aacaattttt gttttaaaca cagggaagag actatagaga tacagagatt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acacaagttc atcggaaaac gaaggagggt aggtgttggt tgggctcccg aagtgaaaat 4380

4321 tgtgttcaag tagccttttg cttcctccca tccacaacca acccgagggc ttcactttta 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SmaI(4436)

4381 agaagagcaa gaatagaata ttagagagag agtgcagaga gggcgggata gctcccggga 4440

4381 tcttctcgtt cttatcttat aatctctctc tcacgtctct cccgccctat cgagggccct 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttccgttttc ttcttcttta tcttcaacga tgatgtgtgt gcgtgttgta tagattctgt 4500

4441 aaggcaaaag aagaagaaat agaagttgct actacacaca cgcacaacat atctaagaca 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgctccccca caactcgctc cgaaggctca atacaattca attgatattg gaggagagcc 4560

4501 acgagggggt gttgagcgag gcttccgagt tatgttaagt taactataac ctcctctcgg 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 taccggagtg ggaggataag aagaaacata agaagaagaa gaagaagaag catgcttctg 4620

4561 atggcctcac cctcctattc ttctttgtat tcttcttctt cttcttcttc gtacgaagac 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gtttttgatg ctatgaaaac ggcacaaaaa gatgattgag gtcccttttc aataccttct 4680

4621 caaaaactac gatacttttg ccgtgttttt ctactaactc cagggaaaag ttatggaaga 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctcatctttc aaatcccatt gaaacctaaa acttctcacc acgctttacc attgttctcc 4740

4681 gagtagaaag tttagggtaa ctttggattt tgaagagtgg tgcgaaatgg taacaagagg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aaaaacttat agcaatgtct ataacttttt tatctctgaa aagcagtgtt ccatttttct 4800

4741 tttttgaata tcgttacaga tattgaaaaa atagagactt ttcgtcacaa ggtaaaaaga 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttttcctatt ttatttcaat tgtttctcac atttcgtttg gattctttgc ttgtcaacca 4860

4801 aaaaggataa aataaagtta acaaagagtg taaagcaaac ctaagaaacg aacagttggt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gcttcttctt ccacttttac cgtctaattt tcagggcagg gagccatcaa acccacgacc 4920

4861 cgaagaagaa ggtgaaaatg gcagattaaa agtcccgtcc ctcggtagtt tgggtgctgg 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 actagatcca tctagaaatg acaagtttgt acaaaaaagc aggcttaatg gcgattgttg 4980

4921 tgatctaggt agatctttac tgttcaaaca tgttttttcg tccgaattac cgctaacaac 4980

.......... .......... ......... ORF 3 attB1 ......... ..........

.......... .......... .......... .......... .......... ..........

4981 cgcaaggagt ttctacgatt ttgacgacgt ttcagaaaac gttcaaagga ttactcccat 5040

4981 gcgttcctca aagatgctaa aactgctgca aagtcttttg caagtttcct aatgagggta 5040

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgataattct tgtggcttac actctattgg gagcatggat cttctggatg atcgaaggag 5100

5041 actattaaga acaccgaatg tgagataacc ctcgtaccta gaagacctac tagcttcctc 5100

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaaacgaacg agagatgctc attgagcaac aaaaagaaag ggatgaactc atccggcgaa 5160

5101 ttttgcttgc tctctacgag taactcgttg tttttctttc cctacttgag taggccgctt 5160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctgtctataa aattaatcaa ttgcaaatca aaagacagag aagattaatg acggccgagg 5220

5161 gacagatatt ttaattagtt aacgtttagt tttctgtctc ttctaattac tgccggctcc 5220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aagaatacaa cagaactgca aaagtgttga ctacctttca agaaactttg ggaattgtac 5280

5221 ttcttatgtt gtcttgacgt tttcacaact gatggaaagt tctttgaaac ccttaacatg 5280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cagctgatat ggacaaggat attcattgga cattcttggg atcaatcttc tattgcatga 5340

5281 gtcgactata cctgttccta taagtaacct gtaagaaccc tagttagaag ataacgtact 5340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cagtctatac aactattggt tacggaaaca ttgttcctgg aactggatgg ggtcgttttg 5400

5341 gtcagatatg ttgataacca atgcctttgt aacaaggacc ttgacctacc ccagcaaaac 5400

.......... .......... ORF 3 .......... .......... ..........

.......... ......... EcoRI(5425) ......... .......... ..........

5401 ctactatcct ttacgcattc attggaattc cactcacagt gctcagtctt tactgtcttg 5460

5401 gatgatagga aatgcgtaag taaccttaag gtgagtgtca cgagtcagaa atgacagaac 5460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gtagtctttt tgccaaggga tgcaaaatgt tgtggagatt ttttttgaag tccaccaggg 5520

5461 catcagaaaa acggttccct acgttttaca acacctctaa aaaaaacttc aggtggtccc 5520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ttgtgagcaa agatttgtca aataagattt ctgaagctgc cgataatatt gaagaaggaa 5580

5521 aacactcgtt tctaaacagt ttattctaaa gacttcgacg gctattataa cttcttcctt 5580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 caacggcaat tactccatca gccgagaaaa cagaaaataa cgatgatgac ttgttgtctt 5640

5581 gttgccgtta atgaggtagt cggctctttt gtcttttatt gctactactg aacaacagaa 5640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tccccatctc cggcctcttg cttattaccg tcatctgggt cattttctgt gcggtcttgt 5700

5641 aggggtagag gccggagaac gaataatggc agtagaccca gtaaaagaca cgccagaaca 5700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tcaccttcct agaagaatgg gactttggaa catctctcta cttcacattg atttctttca 5760

5701 agtggaagga tcttcttacc ctgaaacctt gtagagagat gaagtgtaac taaagaaagt 5760

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 caaccatcgg ttttggagac attcttccat cggattacga tttcatgcca attgttggag 5820

5761 gttggtagcc aaaacctctg taagaaggta gcctaatgct aaagtacggt taacaacctc 5820

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttcttcttct cattggtctt tctttggtct ctacagtgat gacactcatt caacagcaaa 5880

5821 aagaagaaga gtaaccagaa agaaaccaga gatgtcacta ctgtgagtaa gttgtcgttt 5880

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ttgaagcatt agcatcggga atgaaagata acattgacca agaatatgct cgtgcattga 5940

5881 aacttcgtaa tcgtagccct tactttctat tgtaactggt tcttatacga gcacgtaact 5940

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 acgaggctcg tgaagacgga gaggttgacg agcacgtaga tccagaagaa gatccggaga 6000

5941 tgctccgagc acttctgcct ctccaactgc tcgtgcatct aggtcttctt ctaggcctct 6000

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 acaataaaaa gtcctttgac gctgttatct ccagaatgaa ctggtccaag agagggttgt 6060

6001 tgttattttt caggaaactg cgacaataga ggtcttactt gaccaggttc tctcccaaca 6060

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 attatcttct tcctgatagc cagaagaaag aactcgcaaa gcagtctgag aagaaaatgg 6120

6061 taatagaaga aggactatcg gtcttctttc ttgagcgttt cgtcagactc ttcttttacc 6120

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gaagaaaaag tattaaaatt cagactgata acgatttgtt ggaaacactc attcgggagg 6180

6121 cttctttttc ataattttaa gtctgactat tgctaaacaa cctttgtgag taagccctcc 6180

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 aaattttgaa ggcggagctc aacaatgaga tgcacaaata tacagcacca cgatcttcac 6240

6181 tttaaaactt ccgcctcgag ttgttactct acgtgtttat atgtcgtggt gctagaagtg 6240

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... NruI(6271) .......... ..........

6241 accagccaaa acttgtctat tctgatgttc gcgagaagga ggttccaatt gaagttgtcc 6300

6241 tggtcggttt tgaacagata agactacaag cgctcttcct ccaaggttaa cttcaacagg 6300

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gtgttgagca tttcaatcac ggaaatgaag actatctaga gcatgacatc tagtacccag 6360

6301 cacaactcgt aaagttagtg cctttacttc tgatagatct cgtactgtag atcatgggtc 6360

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ctttcttgta caaagtggtg atatctgagc tccgcatcgg ccgctgtcat cagatcgcca 6420

6361 gaaagaacat gtttcaccac tatagactcg aggcgtagcc ggcgacagta gtctagcggt 6420

attB2 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct 6480

6421 agagcgcggg cacggagact gaagattcag gttaatgaga agttgtaggg atgtacgaga 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt 6540

6481 aagagggaca cgagggtggg ggataaaaac aataatagtt ttttgaagag aattaaagaa 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga 6600

6541 acaaaaaatc gaagaaaatt cagtggagat tgttacttta acacatctaa gtttttatct 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc 6660

6601 taattaagca ttatttttca gcttttttta acacgaggga ggggggtaat tattattaag 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat 6720

6661 atagggtttt agatgtgtta caagacacat gtgaagaata caaaaaatga agactattta 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca 6780

6721 aaaaaacttt gtagtatctt ttttggcgtg tgttttatgg aatagtatac aatgcaaagt 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg 6840

6781 caaatactgg cgttaaaaat aaagaagcgt gcagacccgg agagtactgc agtttagtac 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga 6900

6841 gagtagcact ttttcaaaac ctcataaaaa ccttaaaaag ttagttcact ttcaaatact 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg 6960

6901 ttaattaaaa ggacgaaaac gaaaaacccc aaaggggata acaaacagtt ctaaagctcc 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa 7020

6961 tgccgcaaaa agaacgattt tagtgttcat aactactcgt gctacgttct ttctagcctt 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag 7080

7021 cttccaaacc caaactccga gtcaccttcc actcatcttc aactattaaa ctttcacctc 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa 7140

7081 atcacagata ccccaaaaac ggaatttact gtcttatgta agggttatat ggtttgtatt 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ctgtttccaa cttta..... .......... .......... .......... .......... 7155

7141 gacaaaggtt gaaat..... .......... .......... .......... .......... 7155

.......... .......... .......... .......... .......... ..........