Sequence of pCFJ90

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Sequence of pCFJ90

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Sequence of pCFJ90 - Pmyo-2::mCherry::unc-54utr : 5109 BP; 1414 A; 1138 C; 1117 G; 1440 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtatagaaaa gttgcatttt atatctgagt agtatccttt gctttaaatg tccataaaac 60

1 catatctttt caacgtaaaa tatagactca tcataggaaa cgaaatttac aggtattttg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taattttata atcaataaaa caacgtttgt aaatcaactg agtttacaag tagagacatt 120

61 attaaaatat tagttatttt gttgcaaaca tttagttgac tcaaatgttc atctctgtaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gagggatact ttcactatgc taaagtgata atcgaccaaa taataacttc actttggtat 180

121 ctccctatga aagtgatacg atttcactat tagctggttt attattgaag tgaaaccata 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttattcctgt cttataaatg ttatgtatga attaaattca tatgcatatg gctcactctg 240

181 aataaggaca gaatatttac aatacatact taatttaagt atacgtatac cgagtgagac 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 acaattttta ataatcttcc agatcaatat tgactaccga tgcgggtggt cttttgcttt 300

241 tgttaaaaat tattagaagg tctagttata actgatggct acgcccacca gaaaacgaaa 300

.......... .......... .......... .......... .......... ..........

EcoRI(301) .......... .......... .......... .......... ..........

301 gaattctgct gaactttaca ccccgaacag caatgtgtgc ttcagcctaa aaaaaagtaa 360

301 cttaagacga cttgaaatgt ggggcttgtc gttacacacg aagtcggatt ttttttcatt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gtgtgttaat cagtgccccc gattcttcat tttttgcccc tctctcccgt ttcgtcggca 420

361 cacacaatta gtcacggggg ctaagaagta aaaaacgggg agagagggca aagcagccgt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aaagaagaga aaataaagat aagtctcaag ataggttggt aatcgctaaa gtggttgtgt 480

421 tttcttctct tttatttcta ttcagagttc tatccaacca ttagcgattt caccaacaca 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggataagagt agcaaaatgg caggaagagc actttgcgcg cacacactgt actcattgtt 540

481 cctattctca tcgttttacc gtccttctcg tgaaacgcgc gtgtgtgaca tgagtaacaa 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ctggataaaa ttctctcgtt gtttgccgtc ggatgtctgc ctctctgcat tgagccggct 600

541 gacctatttt aagagagcaa caaacggcag cctacagacg gagagacgta actcggccga 600

.......... .......... .......... .......... .......... ..........

.......... HpaI(618) .......... .......... .......... ..........

601 tcttcactat ctttagttaa cctaaaatgc cgtttctttt ctcgtatccc cactatcccg 660

601 agaagtgata gaaatcaatt ggattttacg gcaaagaaaa gagcataggg gtgatagggc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttgaggttct ctgctctctt cgctccctac cgccagcgag caactatccg tgggggcgcc 720

661 aactccaaga gacgagagaa gcgagggatg gcggtcgctc gttgataggc acccccgcgg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttgctcggaa gatggggggg aagaaagaag atttttgcta tttgcacttg agaaagagac 780

721 aacgagcctt ctaccccccc ttctttcttc taaaaacgat aaacgtgaac tctttctctg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttttcctgcg tcgatggtta gagaacagtg tgcagacact tttcagctac ctagaattac 840

781 aaaaggacgc agctaccaat ctcttgtcac acgtctgtga aaagtcgatg gatcttaatg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aattggatat ccccgcctcc caatccaccc acccagggaa aaagaagggc tcgccgaaaa 900

841 ttaacctata ggggcggagg gttaggtggg tgggtccctt tttcttcccg agcggctttt 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tcaaagttat ctccaggctc gcgcatccca ccgagcggtt gacttctctc caccactttt 960

901 agtttcaata gaggtccgag cgcgtagggt ggctcgccaa ctgaagagag gtggtgaaaa 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cattttaacc ctcgatcgtc agacacagaa atgacaagtt tgtacaaaaa agcaggctta 1020

961 gtaaaattgg gagctagcag tctgtgtctt tactgttcaa acatgttttt tcgtccgaat 1020

.......... .......... .......... ......... attB1 ORF 1 .........

.......... .......... .......... .......... .......... ..........

1021 atggtctcaa agggtgaaga agataacatg gcaattatta aagagtttat gcgtttcaag 1080

1021 taccagagtt tcccacttct tctattgtac cgttaataat ttctcaaata cgcaaagttc 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtgcatatgg agggatctgt caatgggcat gagtttgaaa ttgaaggtga aggagaaggc 1140

1081 cacgtatacc tccctagaca gttacccgta ctcaaacttt aacttccact tcctcttccg 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgaccatatg agggaacaca aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta 1200

1141 gctggtatac tcccttgtgt ttggcgtttt gatttccatt caaatttgta tatatatgat 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 actaaccctg attatttaaa ttttcaggta actaaaggcg gaccattacc attcgcctgg 1260

1201 tgattgggac taataaattt aaaagtccat tgatttccgc ctggtaatgg taagcggacc 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gacatcctct ctccacagtt catgtatgga agtaaagctt atgttaaaca tccggcagat 1320

1261 ctgtaggaga gaggtgtcaa gtacatacct tcatttcgaa tacaatttgt aggccgtcta 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ataccagatt atttgaaact ttcattcccg gagggtttta agtgggaacg cgtaatgaat 1380

1321 tatggtctaa taaactttga aagtaagggc ctcccaaaat tcacccttgc gcattactta 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tttgaagacg gaggagttgt tacagtgacg caagactcaa ggtaagttta aacagttcgg 1440

1381 aaacttctgc ctcctcaaca atgtcactgc gttctgagtt ccattcaaat ttgtcaagcc 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tactaactaa ccatacatat ttaaattttc agcctccaag atggagaatt tatttataaa 1500

1441 atgattgatt ggtatgtata aatttaaaag tcggaggttc tacctcttaa ataaatattt 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtcaaacttc gaggaacgaa tttcccctcg gatggacctg ttatgcagaa gaagactatg 1560

1501 cagtttgaag ctccttgctt aaaggggagc ctacctggac aatacgtctt cttctgatac 1560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... ......... AflII(1606) .........

1561 ggatgggaag cttcaagtga aagaatgtac cctgaagacg gtgctcttaa gggagagatt 1620

1561 cctacccttc gaagttcact ttcttacatg ggacttctgc cacgagaatt ccctctctaa 1620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaacaacgtc ttaaattgaa agatggagga cattacgatg ctgaggtaag tttaaacatg 1680

1621 tttgttgcag aatttaactt tctacctcct gtaatgctac gactccattc aaatttgtac 1680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 attttactaa ctaactaatc tgatttaaat tttcaggtga agacaactta caaagccaaa 1740

1681 taaaatgatt gattgattag actaaattta aaagtccact tctgttgaat gtttcggttt 1740

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aaaccagttc agctgccagg agcgtacaat gttaatatta aactggatat cacctcccac 1800

1741 tttggtcaag tcgacggtcc tcgcatgtta caattataat ttgacctata gtggagggtg 1800

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aacgaggatt acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt 1860

1801 ttgctcctaa tgtgatagca actcgttata ctttctcgac ttcccgccgt gagctgtcca 1860

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggcatggatg aattgtataa gtagtaccca gctttcttgt acaaagtggt gatatctgag 1920

1861 ccgtacctac ttaacatatt catcatgggt cgaaagaaca tgtttcacca ctatagactc 1920

.......... ORF 1 .......... attB2 .......... ..........

SacI(1922) EagI(1929) ........ .......... .......... ..........

1921 ctccgcatcg gccgctgtca tcagatcgcc atctcgcgcc cgtgcctctg acttctaagt 1980

1921 gaggcgtagc cggcgacagt agtctagcgg tagagcgcgg gcacggagac tgaagattca 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt 2040

1981 ggttaatgag aagttgtagg gatgtacgag aaagagggac acgagggtgg gggataaaaa 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct 2100

2041 caataatagt tttttgaaga gaattaaaga aacaaaaaat cgaagaaaat tcagtggaga 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa 2160

2101 ttgttacttt aacacatcta agtttttatc ttaattaagc attatttttc agcttttttt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt 2220

2161 aacacgaggg aggggggtaa ttattattaa gatagggttt tagatgtgtt acaagacaca 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca 2280

2221 tgtgaagaat acaaaaaatg aagactattt aaaaaaactt tgtagtatct tttttggcgt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc 2340

2281 gtgttttatg gaatagtata caatgcaaag tcaaatactg gcgttaaaaa taaagaagcg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt 2400

2341 tgcagacccg gagagtactg cagtttagta cgagtagcac tttttcaaaa cctcataaaa 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg 2460

2401 accttaaaaa gttagttcac tttcaaatac tttaattaaa aggacgaaaa cgaaaaaccc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt 2520

2461 caaaggggat aacaaacagt tctaaagctc ctgccgcaaa aagaacgatt ttagtgttca 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag 2580

2521 taactactcg tgctacgttc tttctagcct tcttccaaac ccaaactccg agtcaccttc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg 2640

2581 cactcatctt caactattaa actttcacct catcacagat accccaaaaa cggaatttac 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acagaataca ttcccaatat accaaacata actgtttcca actttattat acatagttga 2700

2641 tgtcttatgt aagggttata tggtttgtat tgacaaaggt tgaaataata tgtatcaact 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 2760

2701 attaagtgac cggcagcaaa atgttgcagc actgaccctt ttgggaccgc aatgggttga 2760

... M13_forward20_pri M13_pUC_fwd_pri lacZ_a ... ..........

.......... .......... .......... .......... .......... ..........

2761 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 2820

2761 attagcggaa cgtcgtgtag ggggaaagcg gtcgaccgca ttatcgcttc tccgggcgtg 2820

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt 2880

2821 gctagcggga agggttgtca acgcgtcgga cttaccgctt accgcggact acgccataaa 2880

.......... lacZ_a .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg 2940

2881 agaggaatgc gtagacacgc cataaagtgt ggcgtatacc acgtgagagt catgttagac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg 3000

2941 gagactacgg cgtatcaatt cggtcggggc tgtgggcggt tgtgggcgac tgcgcgggac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 3060

3001 tgcccgaaca gacgagggcc gtaggcgaat gtctgttcga cactggcaga ggccctcgac 3060

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

3061 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat 3120

3061 gtacacagtc tccaaaagtg gcagtagtgg ctttgcgcgc tctgctttcc cggagcacta 3120

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac 3180

3121 tgcggataaa aatatccaat tacagtacta ttattaccaa agaatctgca gtccaccgtg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 3240

3181 aaaagcccct ttacacgcgc cttggggata aacaaataaa aagatttatg taagtttata 3240

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

3241 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 3300

3241 cataggcgag tactctgtta ttgggactat ttacgaagtt attataactt tttccttctc 3300

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 3360

3301 atactcataa gttgtaaagg cacagcggga ataagggaaa aaacgccgta aaacggaagg 3360

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3361 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 3420

3361 acaaaaacga gtgggtcttt gcgaccactt tcattttcta cgacttctag tcaacccacg 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 3480

3421 tgctcaccca atgtagcttg acctagagtt gtcgccattc taggaactct caaaagcggg 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 3540

3481 gcttcttgca aaaggttact actcgtgaaa atttcaagac gatacaccgc gccataatag 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 3600

3541 ggcataactg cggcccgttc tcgttgagcc agcggcgtat gtgataagag tcttactgaa 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 3660

3601 ccaactcatg agtggtcagt gtcttttcgt agaatgccta ccgtactgtc attctcttaa 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 3720

3661 tacgtcacga cggtattggt actcactatt gtgacgccgg ttgaatgaag actgttgcta 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 3780

3721 gcctcctggc ttcctcgatt ggcgaaaaaa cgtgttgtac cccctagtac attgagcgga 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 3840

3781 actagcaacc cttggcctcg acttacttcg gtatggtttg ctgctcgcac tgtggtgcta 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 3900

3841 cggacatcgt taccgttgtt gcaacgcgtt tgataattga ccgcttgatg aatgagatcg 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 3960

3901 aagggccgtt gttaattatc tgacctacct ccgcctattt caacgtcctg gtgaagacgc 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 4020

3961 gagccgggaa ggccgaccga ccaaataacg actatttaga cctcggccac tcgcacccag 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 4080

4021 agcgccatag taacgtcgtg accccggtct accattcggg agggcatagc atcaatagat 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 4140

4081 gtgctgcccc tcagtccgtt gatacctact tgctttatct gtctagcgac tctatccacg 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 4200

4141 gagtgactaa ttcgtaacca ttgacagtct ggttcaaatg agtatatatg aaatctaact 4200

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 4260

4201 aaattttgaa gtaaaaatta aattttccta gatccacttc taggaaaaac tattagagta 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 4320

4261 ctggttttag ggaattgcac tcaaaagcaa ggtgactcgc agtctggggc atcttttcta 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 4380

4321 gtttcctaga agaactctag gaaaaaaaga cgcgcattag acgacgaacg tttgtttttt 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 4440

4381 tggtggcgat ggtcgccacc aaacaaacgg cctagttctc gatggttgag aaaaaggctt 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 4500

4441 ccattgaccg aagtcgtctc gcgtctatgg tttatgacag gaagatcaca tcggcatcaa 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 4560

4501 tccggtggtg aagttcttga gacatcgtgg cggatgtatg gagcgagacg attaggacaa 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 4620

4561 tggtcaccga cgacggtcac cgctattcag cacagaatgg cccaacctga gttctgctat 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 4680

4621 caatggccta ttccgcgtcg ccagcccgac ttgcccccca agcacgtgtg tcgggtcgaa 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 4740

4681 cctcgcttgc tggatgtggc ttgactctat ggatgtcgca ctcgatactc tttcgcggtg 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 4800

4741 cgaagggctt ccctctttcc gcctgtccat aggccattcg ccgtcccagc cttgtcctct 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 4860

4801 cgcgtgctcc ctcgaaggtc cccctttgcg gaccatagaa atatcaggac agcccaaagc 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 4920

4861 ggtggagact gaactcgcag ctaaaaacac tacgagcagt ccccccgcct cggatacctt 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaacgccagc aacgcggcct ttttacggtt cctggtcttt tgctggcctt ttgctcacat 4980

4921 tttgcggtcg ttgcgccgga aaaatgccaa ggaccagaaa acgaccggaa aacgagtgta 4980

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 5040

4981 caagaaagga cgcaataggg gactaagaca cctattggca taatggcgga aactcactcg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgataccagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct 5100

5041 actatggtcg cctattgtta aagtgtgtcc tttgtcgata ctggtactaa tgcggttcga 5100

....... M13_pUC_rev_pri .... M13_reverse_pri ....... ..........

.......... .......... .......... .......... .......... ..........

5101 atcaacttt. .......... .......... .......... .......... .......... 5109

5101 tagttgaaa. .......... .......... .......... .......... .......... 5109

.......... .......... .......... .......... .......... ..........