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    Plasmid map of pCLXSN

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    Gene/insert name: None
    Insert size (bp): Unknown
    Vector backbone: pCLXSN
    Backbone manufacturer: Verma Lab
    Type of vector: Mammalian expression,Retroviral
    Backbone size (bp): 7807
    5' Sequencing primer: LXSN primer
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Selectable markers: Neomycin
    Plasmid Provided In: DH5a
    Principal Investigator: Inder M Verma
    Comments: A 3.1-kb fragment of LXSN, spanning from the SacI site in the 5'LTR near the U3-R junction to the AccI site just beyond the 3'LTR, was cloned into the 4.5-kb CMV expression construct, CMX-low, at a corresponding SacI site within the CMV immediate early enhancer-promoter.
    Article: The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses. Naviaux RK et al. (J Virol. 1996 Aug . 70(8):5701-5. Pubmed)

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