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pCLXSN
Gene/insert name: None
Insert size (bp): Unknown
Vector backbone: pCLXSN
Backbone manufacturer: Verma Lab
Type of vector: Mammalian expression,Retroviral
Backbone size (bp): 7807
5' Sequencing primer: LXSN primer
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: Neomycin
Plasmid Provided In: DH5a
Principal Investigator: Inder M Verma
Comments: A 3.1-kb fragment of LXSN, spanning from the SacI site in the 5'LTR near the U3-R junction to the AccI site just beyond the 3'LTR, was cloned into the 4.5-kb CMV expression construct, CMX-low, at a corresponding SacI site within the CMV immediate early enhancer-promoter.
Article: The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses. Naviaux RK et al. (J Virol. 1996 Aug . 70(8):5701-5. Pubmed)