Sequence of pCLXSN

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Sequence of pCLXSN

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Sequence of pCLXSN : 7807 BP; 1859 A; 2042 C; 1957 G; 1949 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60

1 gcgcaactgt aactaataac tgatcaataa ttatcattag ttaatgcccc agtaatcaag 60

.......... .......... .... CMV_immearly_promoter .... ..........

.......... .......... .......... .......... .......... ..........

61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120

61 tatcgggtat atacctcaag gcgcaatgta ttgaatgcca tttaccgggc ggaccgactg 120

.......... .... CMV_immearly_promoter ... CAG_enhancer .........

.......... .......... .......... .......... .......... ..........

121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttccatag taacgccaat 180

121 gcgggttgct gggggcgggt aactgcagtt attactgcat acaaggtatc attgcggtta 180

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

181 agggactttc cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt 240

181 tccctgaaag gtaactgcag ttacccacct gataaatgcc atttgacggg tgaaccgtca 240

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 300

241 tgtagttcac atagtatacg gttcatgcgg gggataactg cagttactgc catttaccgg 300

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 360

301 gcggaccgta atacgggtca tgtactggaa taccctgaaa ggatgaaccg tcatgtagat 360

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

361 cgtattagtc atcgctatta ccatgtgatg cggttttggc agtacatcaa tgggcgtgga 420

361 gcataatcag tagcgataat ggtacactac gccaaaaccg tcatgtagtt acccgcacct 420

... CAG_enhancer CMV_immearly_promoter ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 480

421 atcgccaaac tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac 480

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 540

481 aaaaccgtgg ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc 540

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctca ataaaagagc 600

541 gtttacccgc catccgcaca tgccaccctc cagatatatt cgtctcgagt tattttctcg 600

CMV_fwd_primer CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccacaacccc tcactcggcg cgccagtctt ccgatagact gcgtcgcccg ggtacccgta 660

601 ggtgttgggg agtgagccgc gcggtcagaa ggctatctga cgcagcgggc ccatgggcat 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt ccttgggagg 720

661 aagggttatt tcggagaacg acaaacgtag gcttagcacc agagcgacaa ggaaccctcc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg ctcgtccggg 780

721 cagaggagac tcactaactg atgggtgctg cccccagaaa gtaaaccccc gagcaggccc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa 840

781 taaacctctg gggacgggtc cctggtggct gggtggtggc cctccattcg accggtcgtt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc ctgcgtctgt 900

841 gaatagacac agacaggcta acagatcaca gatacaaact acaatacgcg gacgcagaca 900

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 960

901 tgatcaatcg attgatcgag acatagaccg cctgggcacc accttgactg ctcaagactt 960

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 1020

961 gtgggccggc gttgggaccc tctgcagggt ccctgaaacc cccggcaaaa acaccgggct 1020

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 1080

1021 ggactccttc cctcagctac accttaggct ggggcagtcc tatacaccaa gaccatcctc 1080

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 1140

1081 tgctcttgga ttttgtcaag ggcggaggca gacttaaaaa cgaaagccaa accttggctt 1140

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 1200

1141 cggcgcgcag aacagacgac gtcgcgacgt cgtagcaaga cacaacagag acagactgac 1200

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 1260

1201 acaaagacat aaacagactt ttaatcccgg tctgacaatg gtgagggaat tcaaactgga 1260

.......... ........ psi_plus_pack ........ gag ..........

.......... .......... .......... .......... .......... ..........

1261 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 1320

1261 atccagtgac ctttctacag ctcgcctagc gagtgttggt cagccatcta cagttcttct 1320

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 1380

1321 ctgcaaccca atggaagacg agacgtctta ccggttggaa attgcagcct accggcgctc 1380

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 1440

1381 tgccgtggaa attggctctg gagtagtggg tccaattcta gttccagaaa agtggaccgg 1440

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 1500

1441 gcgtacctgt gggtctggtc caggggatgt agcactggac ccttcggaac cgaaaactgg 1500

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 1560

1501 ggggagggac ccagttcggg aaacatgtgg gattcggagg cggaggagaa ggaggtaggc 1560

.......... ...... psi_plus_pack gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 1620

1561 ggggcagaga gggggaactt ggaggagcaa gctggggcgg agctaggagg gaaataggtc 1620

... psi_plus_pack gag MSCV_primer pLXSN_5_primer . pBABE_5_primer

.......... ... EcoRI(1645) BamHI(1661),XhoI(1656) ... ..........

1621 ccctcactcc ttctctaggc gccggaattc gttaactcga ggatccggct gtggaatgtg 1680

1621 gggagtgagg aagagatccg cggccttaag caattgagct cctaggccga caccttacac 1680

gag psi_plus_pack ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 1740

1681 acagtcaatc ccacaccttt caggggtccg aggggtcgtc cgtcttcata cgtttcgtac 1740

pBABE_3_primer .... SV40_enhancer SV40_pro ........ ..........

.......... .......... .......... .......... .......... ..........

1741 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 1800

1741 gtagagttaa tcagtcgttg gtccacacct ttcaggggtc cgaggggtcg tccgtcttca 1800

.......... .... SV40_enhancer SV40_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 1860

1801 tacgtttcgt acgtagagtt aatcagtcgt tggtatcagg gcggggattg aggcgggtag 1860

.......... .. SV40_enhancer SV40_pro SV40_promoter . SV40_origin

.......... .......... .......... .......... .......... ..........

1861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 1920

1861 ggcggggatt gaggcgggtc aaggcgggta agaggcgggg taccgactga ttaaaaaaaa 1920

SV40_enhancer SV40_pro SV40_promoter SV40_origin .. ..........

.......... .......... .......... .......... .......... ..........

1921 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 1980

1921 taaatacgtc tccggctccg gcggagccgg agactcgata aggtcttcat cactcctccg 1980

SV40_origin SV40pro_F_primer SV40_pro SV40_promoter ........

.......... ........ HindIII(2008) ........ .......... ..........

1981 ttttttggag gcctaggctt ttgcaaaaag cttgggctgc aggtcgaggc ggatctgatc 2040

1981 aaaaaacctc cggatccgaa aacgtttttc gaacccgacg tccagctccg cctagactag 2040

........ SV40_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 2100

2041 ttctctgtcc tactcctagc aaagcgtact aacttgttct acctaacgtg cgtccaagag 2100

.......... .......... .......... ........ ORF3 Neomycin ........

.......... .......... .......... .......... .......... ..........

2101 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 2160

2101 gccggcgaac ccacctctcc gataagccga tactgacccg tgttgtctgt tagccgacga 2160

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 2220

2161 gactacggcg gcacaaggcc gacagtcgcg tccccgcggg ccaagaaaaa cagttctggc 2220

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 2280

2221 tggacaggcc acgggactta cttgacgtcc tgctccgtcg cgccgatagc accgaccggt 2280

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 2340

2281 gctgcccgca aggaacgcgt cgacacgagc tgcaacagtg acttcgccct tccctgaccg 2340

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 2400

2341 acgataaccc gcttcacggc cccgtcctag aggacagtag agtggaacga ggacggctct 2400

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 2460

2401 ttcataggta gtaccgacta cgttacgccg ccgacgtatg cgaactaggc cgatggacgg 2460

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 2520

2461 gtaagctggt ggttcgcttt gtagcgtagc tcgctcgtgc atgagcctac cttcggccag 2520

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 2580

2521 aacagctagt cctactagac ctgcttctcg tagtccccga gcgcggtcgg cttgacaagc 2580

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 2640

2581 ggtccgagtt ccgcgcgtac gggctgccgc tcctagagca gcactgggta ccgctacgga 2640

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 2700

2641 cgaacggctt atagtaccac cttttaccgg cgaaaagacc taagtagctg acaccggccg 2700

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 2760

2701 acccacaccg cctggcgata gtcctgtatc gcaaccgatg ggcactataa cgacttctcg 2760

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 2820

2761 aaccgccgct tacccgactg gcgaaggagc acgaaatgcc atagcggcga gggctaagcg 2820

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 2880

2821 tcgcgtagcg gaagatagcg gaagaactgc tcaagaagac tcgccctgag accccaagct 2880

.......... Neomycin ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta 2940

2881 attttatttt ctaaaataaa tcagaggtct ttttcccccc ttactttctg gggtggacat 2940

.......... .......... .......... ......... 3MoMuLV_LTR .........

.......... NheI(2951) .......... .......... .......... ..........

2941 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga 3000

2941 ccaaaccgtt cgatcgaatt cattgcggta aaacgttccg taccttttta tgtattgact 3000

.......... ......... 3MoMuLV_LTR ......... 5_LTR2 ..........

.......... .......... .......... .......... .......... ..........

3001 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca 3060

3001 cttatctctt caagtctagt tccagtcctt gtctaccttg tcgacttata cccggtttgt 3060

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg 3120

3061 cctatagaca ccattcgtca aggacggggc cgagtcccgg ttcttgtcta ccttgtcgac 3120

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa 3180

3121 ttatacccgg tttgtcctat agacaccatt cgtcaaggac ggggccgagt cccggttctt 3180

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... XbaI(3218) .......... ..........

3181 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc 3240

3181 gtctaccagg ggtctacgcc aggtcgggag tcgtcaaaga tctcttggta gtctacaaag 3240

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg 3300

3241 gtcccacggg gttcctggac tttactggga cacggaataa acttgattgg ttagtcaagc 3300

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc 3360

3301 gaagagcgaa gacaagcgcg cgaagacgag gggctcgagt tattttctcg ggtgttgggg 3360

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa 3420

3361 agtgagcccc gcggtcagga ggctaactga ctcagcgggc ccatgggcac ataggttatt 3420

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga 3480

3421 tgggagaacg tcaacgtagg ctgaacacca gagcgacaag gaaccctccc agaggagact 3480

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac 3540

3481 cactaactga tgggcagtcg cccccagaaa gtaaaccccc gagcaggccc tagccctctg 3540

...... 3MoMuLV_LTR 5_LTR2 ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg 3600

3541 gggacgggtc cctggtggct gggtggtggc cctccattcg accgacggag cgcgcaaagc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 3660

3601 cactactgcc acttttggag actgtgtacg tcgagggcct ctgccagtgt cgaacagaca 3660

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 3720

3661 ttcgcctacg gccctcgtct gttcgggcag tcccgcgcag tcgcccacaa ccgcccacag 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatacctag ctaggtagct 3780

3721 ccccgcgtcg gtactgggtc agtgcatcgc tatcgcctca catatggatc gatccatcga 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta 3840

3781 tctcctagaa acacttcctt ggaatgaaga caccacactg tattaacctg tttgatggat 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac 3900

3841 gtctctaaat ttcgagattc catttatatt ttaaaaattc acatattaca caatttgatg 3900

.......... .......... SV40_int ........ SV40_3_splice ........

.......... .......... .......... .......... .......... ..........

3901 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt 3960

3901 actaagatta acaaacacat aaaatctaag gttggatacc ttgactactt accctcgtca 3960

........ SV40_3_splice ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ggtccaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcct ctagtgatga 4020

3961 ccaggttacg gaaattactc cttttggaca aaacgagtct tctttacgga gatcactact 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga 4080

4021 actccgatga cgactgagag ttgtaagatg aggaggtttt ttcttctctt tccatcttct 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag 4140

4081 ggggttcctg aaaggaagtc ttaacgattc aaaaaactca gtacgacaca aatcattatc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa 4200

4141 ttgagaacga acgaaacgat aaatgtggtg tttccttttt cgacgtgacg atatgttctt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aattatggaa aaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca 4260

4201 ttaatacctt tttataaact acatatcacg gaactgatct ctagtattag tcggtatggt 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 4320

4261 gtaaacatct ccaaaatgaa cgaaattttt tggagggtgt ggagggggac ttggactttg 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 4380

4321 tattttactt acgttaacaa caacaattga acaaataacg tcgaatatta ccaatgttta 4380

.......... .......... .......... ...... SV40_PA_terminator ......

.......... .......... .......... .......... .......... ..........

4381 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 4440

4381 tttcgttatc gtagtgttta aagtgtttat ttcgtaaaaa aagtgacgta agatcaacac 4440

.......... ...... SV40_PA_terminator ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtttgtccaa actcatcaat gtatcttatc atgtctgatc ccgccatggt atcaacgcca 4500

4441 caaacaggtt tgagtagtta catagaatag tacagactag ggcggtacca tagttgcggt 4500

EBV_rev_primer SV40_PA_terminator ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tatttctatt tacagtaggg acctcttcgt tgtgtaggta ccgctgtatt cctagggaaa 4560

4501 ataaagataa atgtcatccc tggagaagca acacatccat ggcgacataa ggatcccttt 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tagtagaggc accttgaact gtctgcatca gccatatagc ccccgctgtt cgacttacaa 4620

4561 atcatctccg tggaacttga cagacgtagt cggtatatcg ggggcgacaa gctgaatgtt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acacaggcac agtactgaca aacccataca cctcctctga aatacccata gttgctaggg 4680

4621 tgtgtccgtg tcatgactgt ttgggtatgt ggaggagact ttatgggtat caacgatccc 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctgtctccga actcattaca ccctccaaag tcagagctgt aatttcgcca tcaagggcag 4740

4681 gacagaggct tgagtaatgt gggaggtttc agtctcgaca ttaaagcggt agttcccgtc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cgagggcttc tccagataaa atagcttctg ccgagagtcc cgtaagggta gacacttcag 4800

4741 gctcccgaag aggtctattt tatcgaagac ggctctcagg gcattcccat ctgtgaagtc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctaatccctc gatgaggtct actagaatag tcagtgcggc tcccattttg aaaattcact 4860

4801 gattagggag ctactccaga tgatcttatc agtcacgccg agggtaaaac ttttaagtga 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tacttgatca gcttcagaag atggcggagg gcctccaaca cagtaatttt cctcccgact 4920

4861 atgaactagt cgaagtcttc taccgcctcc cggaggttgt gtcattaaaa ggagggctga 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cttaaaatag aaaatgtcaa gtcagttaag caggaagtgg actaactgac gcagctggcc 4980

4921 gaattttatc ttttacagtt cagtcaattc gtccttcacc tgattgactg cgtcgaccgg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gtgcgacatc ctcttttaat tagttgctag gcaacgccct ccagagggcg tgtggttttg 5040

4981 cacgctgtag gagaaaatta atcaacgatc cgttgcggga ggtctcccgc acaccaaaac 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 caagaggaag caaaagcctc tccacccagg cctagaatgt ttccacccaa tcattactat 5100

5041 gttctccttc gttttcggag aggtgggtcc ggatcttaca aaggtgggtt agtaatgata 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gacaacagct gtttttttta gtattaagca gaggccgggg acccctggcc cgcttactct 5160

5101 ctgttgtcga caaaaaaaat cataattcgt ctccggcccc tggggaccgg gcgaatgaga 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggagaaaaaa aacattgtag aggcttccag aggcaacttg tcaaaacagg actgcttcta 5220

5161 cctctttttt ttgtaacatc tccgaaggtc tccgttgaac agttttgtcc tgacgaagat 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tttctgtcac actgtctggc cctgtcacaa ggtccagcac ctccataccc cctttaataa 5280

5221 aaagacagtg tgacagaccg ggacagtgtt ccaggtcgtg gaggtatggg ggaaattatt 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcagtttggg aacgggtgcg ggtcttactc cgcccatccg cccctaactc cgcccagttc 5340

5281 cgtcaaaccc ttgcccacgc ccagaatgag gcgggtaggc ggggattgag gcgggtcaag 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cgcccattct ccgccccatg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 5400

5341 gcgggtaaga ggcggggtac gactgattaa aaaaaataaa tacgtctccg gctccggcgg 5400

.......... .......... .......... ....... SV40pro_F_primer .......

.......... .......... .......... .......... .......... ..........

5401 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggctg cattaatgaa 5460

5401 agccggagac tcgataaggt cttcatcact cctccgaaaa aacctccgac gtaattactt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 5520

5461 agccggttgc gcgcccctct ccgccaaacg cataacccgc gagaaggcga aggagcgagt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 5580

5521 gactgagcga cgcgagccag caagccgacg ccgctcgcca tagtcgagtg agtttccgcc 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 5640

5581 attatgccaa taggtgtctt agtcccctat tgcgtccttt cttgtacact cgttttccgg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 5700

5641 tcgttttccg gtccttggca tttttccggc gcaacgaccg caaaaaggta tccgaggcgg 5700

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 5760

5701 ggggactgct cgtagtgttt ttagctgcga gttcagtctc caccgctttg ggctgtcctg 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 5820

5761 atatttctat ggtccgcaaa gggggacctt cgagggagca cgcgagagga caaggctggg 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 5880

5821 acggcgaatg gcctatggac aggcggaaag agggaagccc ttcgcaccgc gaaagagtat 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5940

5881 cgagtgcgac atccatagag tcaagccaca tccagcaagc gaggttcgac ccgacacacg 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 6000

5941 tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc attgatagca gaactcaggt 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 6060

6001 tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc taatcgtctc 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 6120

6061 gctccataca tccgccacga tgtctcaaga acttcaccac cggattgatg ccgatgtgat 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 6180

6121 cttcctgtca taaaccatag acgcgagacg acttcggtca atggaagcct ttttctcaac 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 6240

6181 catcgagaac taggccgttt gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 6300

6241 tcgtctaatg cgcgtctttt tttcctagag ttcttctagg aaactagaaa agatgcccca 6300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 6360

6301 gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa ccagtactct aatagttttt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 6420

6361 cctagaagtg gatctaggaa aatttaattt ttacttcaaa atttagttag atttcatata 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 6480

6421 tactcatttg aaccagactg tcaatggtta cgaattagtc actccgtgga tagagtcgct 6480

.......... .......... .......... .......... Ampicillin ..........

.......... .......... .......... .......... .......... ..........

6481 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 6540

6481 agacagataa agcaagtagg tatcaacgga ctgaggggca gcacatctat tgatgctatg 6540

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 6600

6541 ccctcccgaa tggtagaccg gggtcacgac gttactatgg cgctctgggt gcgagtggcc 6600

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 6660

6601 gaggtctaaa tagtcgttat ttggtcggtc ggccttcccg gctcgcgtct tcaccaggac 6660

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 6720

6661 gttgaaatag gcggaggtag gtcagataat taacaacggc ccttcgatct cattcatcaa 6720

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct 6780

6721 gcggtcaatt atcaaacgcg ttgcaacaac ggtaacgacg tccgtagcac cacagtgcga 6780

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 6840

6781 gcagcaaacc ataccgaagt aagtcgaggc caagggttgc tagttccgct caatgtacta 6840

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 6900

6841 gggggtacaa cacgtttttt cgccaatcga ggaagccagg aggctagcaa cagtcttcat 6900

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 6960

6901 tcaaccggcg tcacaatagt gagtaccaat accgtcgtga cgtattaaga gaatgacagt 6960

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tgccatccgt aagatgcttt tctgtgactg gtgaactcaa ccaagtcatt ctgagaatag 7020

6961 acggtaggca ttctacgaaa agacactgac cacttgagtt ggttcagtaa gactcttatc 7020

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 7080

7021 acatacgccg ctggctcaac gagaacgggc cgcagttatg ccctattatg gcgcggtgta 7080

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 7140

7081 tcgtcttgaa attttcacga gtagtaacct tttgcaagaa gccccgcttt tgagagttcc 7140

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 7200

7141 tagaatggcg acaactctag gtcaagctac attgggtgag cacgtgggtt gactagaagt 7200

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 7260

7201 cgtagaaaat gaaagtggtc gcaaagaccc actcgttttt gtccttccgt tttacggcgt 7260

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 7320

7261 tttttccctt attcccgctg tgcctttaca acttatgagt atgagaagga aaaagttata 7320

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 7380

7321 ataacttcgt aaatagtccc aataacagag tactcgccta tgtataaact tacataaatc 7380

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

7381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 7440

7381 tttttatttg tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg actgcagatt 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 7500

7441 ctttggtaat aatagtactg taattggata tttttatccg catagtgctc cgggaaagca 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 7560

7501 gagcgcgcaa agccactact gccacttttg gagactgtgt acgtcgaggg cctctgccag 7560

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

7561 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 7620

7561 tgtcgaacag acattcgcct acggccctcg tctgttcggg cagtcccgcg cagtcgccca 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg 7680

7621 caaccgccca cagccccgac cgaattgata cgccgtagtc tcgtctaaca tgactctcac 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc 7740

7681 gtggtatacg ccacacttta tggcgtgtct acgcattcct cttttatggc gtagtccgcg 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 7800

7741 gtaagcggta agtccgacgc gttgacaacc cttcccgcta gccacgcccg gagaagcgat 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ttacgcc... .......... .......... .......... .......... .......... 7807

7801 aatgcgg... .......... .......... .......... .......... .......... 7807

.......... .......... .......... .......... .......... ..........