Sequence of pCM5.43

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Sequence of pCM5.43

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Sequence of pCM5.43 : 3139 BP; 811 A; 715 C; 701 G; 912 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 120

61 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... PvuII(174)

121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 180

121 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 240

181 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattat gcgcatggcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 300

241 atcggtcctt ctcaaacatc tttgcgtttt tccggtaggc agtcctaccg gaagacgaat 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 360

301 caaactacgg accgtcaaat accgcccgca ggacgggcgg tgggaggccc ggcaacgaag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 420

361 tgttgcaagt ttaggcgagg gccgcctaaa caggatgagt cctctcgcaa gtggctgttt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 480

421 gttgtctatt ttgctttccg ggtcagaagg ctgactcgga aagcaaaata aactacggac 480

.......... ......... rrnB_T1_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 540

481 cgtcaaggga tgagagcgca attgcgatcg tacctacaaa agggtcagtg ctgcaacatt 540

.......... .......... .......... .......... ....... M13_pUC_fwd_pri

.......... .......... .......... .......... .......... ..........

541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 600

541 ttgctgccgg tcagaattcg agcccgggac gtcgagatct cgagcttaag atgtccagtg 600

M13_forward20_pri .. .......... .......... .......... ..........

.......... .......... .......... NdeI(636) .......... ..........

601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg 660

601 attatggtag attcatcaac taagtatcac tgacgtatac aacacaaaat gtcataatac 660

.......... .......... .......... .......... attR2 ..........

.......... .......... .......... .......... .......... ..........

661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 720

661 atcagacaaa aaatacgttt tagattaaat tatataacta taaatatagt aaaatgcaaa 720

.......... .......... attR2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctcgttcaac tttcttgtac aaagtgggat aaatacgaat tctacatttt tgtcattagt 780

721 gagcaagttg aaagaacatg tttcacccta tttatgctta agatgtaaaa acagtaatca 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 attttctgtc ctttaggtag ttttctattc cattgctctg gtgcctacca aataagaatt 840

781 taaaagacag gaaatccatc aaaagataag gtaacgagac cacggatggt ttattcttaa 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aaaacacgta ttttgtatcc tatacgcaac tgtatatttg attcaaatac tagaattagc 900

841 ttttgtgcat aaaacatagg atatgcgttg acatataaac taagtttatg atcttaatcg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tagtttcttt ctctttttcc tatccaaatt atcttcctta cgaatattgt tccattctat 960

901 atcaaagaaa gagaaaaagg ataggtttaa tagaaggaat gcttataaca aggtaagata 960

.......... .......... .......... .......... .......... ..........

.......... ClaI(979) .......... .......... .......... ..........

961 aatgtgcttc tgaagccatc gatcttttca tatcttctag taagattgaa tttagatccc 1020

961 ttacacgaag acttcggtag ctagaaaagt atagaagatc attctaactt aaatctaggg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 acgttcccca ttcccatagt attcaaatgt ttttgtatgc ccccaattta gaataaaatg 1080

1021 tgcaaggggt aagggtatca taagtttaca aaaacatacg ggggttaaat cttattttac 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aaatcacaat aatgacacca gtagcccctc tctcgcatta catggtaatt ttgaatcacc 1140

1081 tttagtgtta ttactgtggt catcggggag agagcgtaat gtaccattaa aacttagtgg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 caaattgtcc actttaacac ctaaaaaatc cgaagtagaa ttttgtgtgt gattttaata 1200

1141 gtttaacagg tgaaattgtg gattttttag gcttcatctt aaaacacaca ctaaaattat 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aatcttttcg cagagaagat tgtgacttaa gtgtttttct tatttttcaa ctttattata 1260

1201 ttagaaaagc gtctcttcta acactgaatt cacaaaaaga ataaaaagtt gaaataatat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 caaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcactat 1320

1261 gtttcaaccg taatattttt tcgtaacgaa tagttaaaca acgttgcttg tccagtgata 1320

.......... .......... .......... .......... .......... ..........

.......... .......... NcoI(1350) ... NotI(1367),EagI(1367) EcoRV(1376)

1321 cagtcaaaat aaaatcatta tttggagctc catggtagcg ttaacgcggc cgcgatatcc 1380

1321 gtcagtttta ttttagtaat aaacctcgag gtaccatcgc aattgcgccg gcgctatagg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc 1440

1381 ggatatcact cagcataatg taccagtatc gacaaaggac cgtcgagacc gggcacagag 1440

T7_pro ....... M13_reverse_pri ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt 1500

1441 ttttagagac tacaatgtaa cgtgttctat ttttatatag tagtacttgt tattttgaca 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga 1560

1501 gacgaatgta tttgtcatta tgttccccac aatactcggt ataagttgcc ctttgcagct 1560

.......... .......... .......... ......... ORF 3 KanR2 .........

.......... .......... .......... .......... .......... NruI(1615)

1561 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 1620

1561 ccggcgctaa tttaaggttg tacctacgac taaatatacc catatttacc cgagcgctat 1620

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt 1680

1621 tacagcccgt tagtccacgc tgttagatag cgaacatacc cttcgggcta cgcggtctca 1680

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 1740

1681 acaaagactt tgtaccgttt ccatcgcaac ggttactaca atgtctactc taccagtctg 1740

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 1800

1741 atttgaccga ctgccttaaa tacggagaag gctggtagtt cgtaaaatag gcatgaggac 1800

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag 1860

1801 tactacgtac caatgagtgg tgacgctagg ggcctttttg tcgtaaggtc cataatcttc 1860

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 1920

1861 ttataggact aagtccactt ttataacaac tacgcgaccg tcacaaggac gcggccaacg 1920

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 1980

1921 taagctaagg acaaacatta acaggaaaat tgtcgctagc gcataaagca gagcgagtcc 1980

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 2040

1981 gcgttagtgc ttacttattg ccaaaccaac tacgctcact aaaactactg ctcgcattac 2040

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt 2100

2041 cgaccggaca acttgttcag acctttcttt acgtatttga aaacggtaag agtggcctaa 2100

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 2160

2101 gtcagcagtg agtaccacta aagagtgaac tattggaata aaaactgctc ccctttaatt 2160

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 2220

2161 atccaacata actacaacct gctcagcctt agcgtctggc tatggtccta gaacggtagg 2220

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 2280

2221 ataccttgac ggagccactc aaaagaggaa gtaatgtctt tgccgaaaaa gtttttatac 2280

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 2340

2281 cataactatt aggactatac ttatttaacg tcaaagtaaa ctacgagcta ctcaaaaaga 2340

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg 2400

2341 ttagtcttaa ccaattaacc aacattgtga ccgtctcgta atgcgactga actgccctgc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag 2460

2401 cgcgttcgag tactggtttt agggaattgc actcaatgcg cagcaaggtg actcgcagtc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 2520

2461 tggggcatct tttctagttt cctagaagaa ctctaggaaa aaaagacgcg cattagacga 2520

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2521 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 2580

2521 cgaacgtttg tttttttggt ggcgatggtc gccaccaaac aaacggccta gttctcgatg 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 2640

2581 gttgagaaaa aggcttccat tgaccgaagt cgtctcgcgt ctatggttta tgacaggaag 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 2700

2641 atcacatcgg catcaatccg gtggtgaagt tcttgagaca tcgtggcgga tgtatggagc 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 2760

2701 gagacgatta ggacaatggt caccgacgac ggtcaccgct attcagcaca gaatggccca 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... ......... ApaLI(2819)

2761 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 2820

2761 acctgagttc tgctatcaat ggcctattcc gcgtcgccag cccgacttgc cccccaagca 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 2880

2821 cgtgtgtcgg gtcgaacctc gcttgctgga tgtggcttga ctctatggat gtcgcactcg 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 2940

2881 taactctttc gcggtgcgaa gggcttccct ctttccgcct gtccataggc cattcgccgt 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 3000

2941 cccagccttg tcctctcgcg tgctccctcg aaggtccccc tttgcggacc atagaaatat 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 3060

3001 caggacagcc caaagcggtg gagactgaac tcgcagctaa aaacactacg agcagtcccc 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 3120

3061 ccgcctcgga tacctttttg cggtcgttgc gccggaaaaa tgccaaggac cggaaaacga 3120

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggccttttgc tcacatgtt. .......... .......... .......... .......... 3139

3121 ccggaaaacg agtgtacaa. .......... .......... .......... .......... 3139

.......... .......... .......... .......... .......... ..........