Sequence of pCMV-Sport

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Sequence of pCMV-Sport_1

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Sequence of pCMV-Sport 1 : 4380 BP; 1176 A; 1050 C; 1042 G; 1112 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 60

1 ggtaagcggt aagtccgacg cgttgacaac ccttcccgct agccacgccc ggagaagcga 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 120

61 taatgcggtc ggttatgcgt ttggcggaga ggggcgcgca accggctaag taattacgtc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 180

121 ctagctaggt ctgtactatt ctatgtaact actcaaacct gtttggtgtt gatcttacgt 180

.......... .......... ... EBV_rev_pri SV40_PA_term ... ..........

.......... .......... .......... .......... .......... ..........

181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 240

181 cacttttttt acgaaataaa cactttaaac actacgataa cgaaataaac attggtaata 240

.......... ......... SV40_PA_term ......... .......... ..........

.......... HpaI(259) .......... .......... .......... ..........

241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 300

241 ttcgacgtta tttgttcaat tgttgttgtt aacgtaagta aaatacaaag tccaagtccc 300

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta 360

301 cctccacacc ctccaaaaaa tttcgttcat tttggagatg tttacaccat accgactaat 360

.......... .......... .......... .......... .......... ..........

BclI(361) .......... .......... .......... .......... ..........

361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct 420

361 actagtactt gtctgacact cctgactccc cggactttac tcggaaccct gacacttaga 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact 480

421 ttttatgtgt ttgttaatct tagtgatcga ggacacatat tataaaagta tttagtatga 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta 540

481 gtcattcgtt ttgagagttc gtcgttcgta tacgtcgatc aaattgtgta atatgtgaat 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca 600

541 ttttaaaata taaatggaat ctcgaaattt agagacatcc atcaaacagg ttaatacagt 600

SV40_int .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcttataata cgactcacta 660

601 gtggtgtctt cattccaagg aagtgtttct agggttcgat cgaatattat gctgagtgat 660

.......... .......... .......... .......... .......... T7_pro

.......... .......... .... HindIII(694) ApaI(704),XhoI(706) BamHI(712)

661 tagggagaga gctatgacgt cgcatgcacg cgtaagcttg ggcccctcga gggatcctct 720

661 atccctctct cgatactgca gcgtacgtgc gcattcgaac ccggggagct ccctaggaga 720

.......... .......... .......... .......... .......... ..........

NotI,EagI SpeI SalI SmaI,EcoRI AgeI KpnI,PstI777)

721 agagcggccg ccgactagtg agctcgtcga cccgggaatt ccggaccggt acctgcaggc 780

721 tctcgccggc ggctgatcac tcgagcagct gggcccttaa ggcctggcca tggacgtccg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtaccttcta tagtgtcacc taaatagctt tttgcaaaag cctaggctag agtccggagg 840

781 catggaagat atcacagtgg atttatcgaa aaacgttttc ggatccgatc tcaggcctcc 840

.......... Sp6_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg 900

841 gacctagcca gggccacaga agatacctcc agttttgtcg cacctaccgc agaggtccgc 900

.......... .......... CMV2_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg 960

901 tagactgcca agtgatttgc tcgagacgaa tatatctgga gggtggcatg tgcggatggc 960

LNCX_pri pCEP_fwd_pri CMV2_pro CMV_pro CMV_immearly_pro CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

961 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg 1020

961 gggtaaacgc agttaccccg cctcaacaat gctgtaaaac ctttcagggc aactaaaacc 1020

CMV_pro CMV_fwd_pri CMV_immearly_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca 1080

1021 acggttttgt ttgagggtaa ctgcagttac cccacctctg aacctttagg ggcactcagt 1080

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... NcoI(1124) ..........

1081 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg 1140

1081 ttggcgatag gtgcgggtaa ctacatgacg gttttggcgt agtggtacca ttatcgctac 1140

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa 1200

1141 tgattatgca tctacatgac ggttcatcct ttcagggtat tccagtacat gacccgtatt 1200

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac 1260

1201 acggtccgcc cggtaaatgg cagtaactgc agttatcccc cgcatgaacc gtatactatg 1260

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg 1320

1261 tgaactacat gacggttcac ccgtcaaatg gcatttatga ggtgggtaac tgcagttacc 1320

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg 1380

1321 tttcagggat aaccgcaatg atacccttgt atgcagtaat aactgcagtt acccgccccc 1380

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgac ctgcacgatg 1440

1381 agcaacccgc cagtcggtcc gcccggtaaa tggcattcaa tacattgctg gacgtgctac 1440

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccggaagc 1500

1441 gacaaaggac acactttaac aataggcgag tgttaaggtg tgtaatatgc tcggccttcg 1500

....... M13_pUC_rev_pri ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tataaagtgt aaagcctggg gtgcctaatg agtgaaaggg cctcgtatac gcctattttt 1560

1501 atatttcaca tttcggaccc cacggattac tcactttccc ggagcatatg cggataaaaa 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa 1620

1561 tatccaatta cagtactatt attaccaaag aatctgcagt ccaccgtgaa aagccccttt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 1680

1621 acacgcgcct tggggataaa caaataaaaa gatttatgta agtttataca taggcgagta 1680

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

1681 gagacaataa ccctgataaa tgcttcaata atattgaaaa acgcgcgaat tgcaagctct 1740

1681 ctctgttatt gggactattt acgaagttat tataactttt tgcgcgctta acgttcgaga 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 1800

1741 cgtaattact tagccggttg cgcgcccctc tccgccaaac gcataacccg cgagaaggcg 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 1860

1801 aaggagcgag tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 1920

1861 gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 1980

1921 tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt 1980

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 2040

1981 atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 2100

2041 gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2160

2101 acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2220

2161 cgaaagagtt acgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 2280

2221 cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 2340

2281 agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 2400

2341 ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 2460

2401 gccgatgtga tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 2520

2461 tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 2580

2521 acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgcc 2640

2581 aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa accagtacgg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ataacttcgt atagcataca ttatacgaag ttatggcatg agattatcaa aaaggatctt 2700

2641 tattgaagca tatcgtatgt aatatgcttc aataccgtac tctaatagtt tttcctagaa 2700

.......... loxP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 2760

2701 gtggatctag gaaaatttaa tttttacttc aaaatttagt tagatttcat atatactcat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 2820

2761 ttgaaccaga ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc gctagacaga 2820

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2821 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 2880

2821 taaagcaagt aggtatcaac ggactgaggg gcagcacatc tattgatgct atgccctccc 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 2940

2881 gaatggtaga ccggggtcac gacgttacta tggcgctctg ggtgcgagtg gccgaggtct 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 3000

2941 aaatagtcgt tatttggtcg gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 3060

3001 taggcggagg taggtcagat aattaacaac ggcccttcga tctcattcat caagcggtca 3060

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 3120

3061 attatcaaac gcgttgcaac aacggtaacg atgtccgtag caccacagtg cgagcagcaa 3120

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 3180

3121 accataccga agtaagtcga ggccaagggt tgctagttcc gctcaatgta ctagggggta 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 3240

3181 caacacgttt tttcgccaat cgaggaagcc aggaggctag caacagtctt cattcaaccg 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 3300

3241 gcgtcacaat agtgagtacc aataccgtcg tgacgtatta agagaatgac agtacggtag 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 3360

3301 gcattctacg aaaagacact gaccactcat gagttggttc agtaagactc ttatcacata 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 3420

3361 cgccgctggc tcaacgagaa cgggccgcag ttatgcccta ttatggcgcg gtgtatcgtc 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 3480

3421 ttgaaatttt cacgagtagt aaccttttgc aagaagcccc gcttttgaga gttcctagaa 3480

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 3540

3481 tggcgacaac tctaggtcaa gctacattgg gtgagcacgt gggttgacta gaagtcgtag 3540

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 3600

3541 aaaatgaaag tggtcgcaaa gacccactcg tttttgtcct tccgttttac ggcgtttttt 3600

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 3660

3601 cccttattcc cgctgtgcct ttacaactta tgagtatgag aaggaaaaag ttataataac 3660

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aagcatttat cagggttatt gtctcatgcc aggggtgggc acacatattt gataccagcg 3720

3661 ttcgtaaata gtcccaataa cagagtacgg tccccacccg tgtgtataaa ctatggtcgc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 atccctacac agcacataat tcaatgcgac ttccctctat cgcacatctt agacctttat 3780

3721 tagggatgtg tcgtgtatta agttacgctg aagggagata gcgtgtagaa tctggaaata 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tctccctcca gcacacatcg aagctgccga gcaagccgtt ctcaccagtc caagacctgg 3840

3781 agagggaggt cgtgtgtagc ttcgacggct cgttcggcaa gagtggtcag gttctggacc 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 3900

3841 gtactcgcct atgtataaac ttacataaat ctttttattt gtttatcccc aaggcgcgtg 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt 3960

3901 taaaggggct tttcacggtg gactttaaca tttgcaatta taaaacaatt ttaagcgcaa 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 4020

3961 tttaaaaaca atttagtcga gtaaaaaatt ggttatccgg ctttagccgt tttagggaat 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 4080

4021 atttagtttt cttatctggc tctatcccaa ctcacaacaa ggtcaaacct tgttctcagg 4080

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

4081 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 4140

4081 tgataatttc ttgcacctga ggttgcagtt tcccgctttt tggcagatag tcccgctacc 4140

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 4200

4141 gggtgatgca cttggtagtg ggattagttc aaaaaacccc agctccacgg catttcgtga 4200

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 4260

4201 tttagccttg ggatttccct cgggggctaa atctcgaact gcccctttcg gccgcttgca 4260

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 4320

4261 ccgctctttc cttcccttct ttcgctttcc tcgcccgcga tcccgcgacc gttcacatcg 4320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 4380

4321 ccagtgcgac gcgcattggt ggtgtgggcg gcgcgaatta cgcggcgatg tcccgcgcag 4380

.......... .......... f1_origin .......... .......... ..........