YYx LOCUS pCMV-Sport 6 4396 bp DNA SYN 24-Aug-2007 DEFINITION pCMV-Sport 6 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4396 /organism="pCMV-Sport 6" /mol_type="other DNA" promoter 16..34 /label="T7_promoter" misc_feature complement(36..60) /label="attB2" misc_feature complement(153..177) /label="attB1" misc_feature complement(179..196) /label="Sp6_primer" misc_feature complement(220..242) /label="M13_pUC_rev_primer" promoter complement(266..385) /label="CMV2_promoter" misc_feature complement(328..352) /label="LNCX_primer" misc_feature complement(335..354) /label="pCEP_fwd_primer" promoter complement(328..399) /label="CMV_promoter" misc_feature complement(378..398) /label="CMV_fwd_primer" promoter complement(351..855) /label="CMV_immearly_promoter" misc_feature complement(564..851) /label="CAG_enhancer" promoter 1001..1029 /label="AmpR_promoter" rep_origin complement(1301..1920) /label="pBR322_origin" misc_feature 1982..2015 /label="loxP" CDS complement(2117..2977) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2117..2977) /label="Ampicillin" /gene="Ampicillin" rep_origin complement(3397..3703) /label="f1_origin" misc_feature complement(3870..3889) /label="EBV_rev_primer" terminator complement(3856..3975) /label="SV40_PA_terminator" misc_feature complement(4263..4278) /label="SV40_int" misc_feature 4370..4392 /label="M13_pUC_fwd_primer" misc_feature 4385..5 /label="M13_forward20_primer" ORIGIN 1 CCAGTGCCTA GCTTATAATA CGACTCACTA TAGGGACCAC TTTGTACAAG AAAGCTGGGT 61 ACGCGTAAGC TTGGGCCCCT CGAGGGATAC TCTAGAGCGG CCGCCGACTA GTGAGCTCGT 121 CGACGATATC CCGGGAATTC CGGACCGGTA CCAGCCTGCT TTTTTGTACA AACTTGTTCT 181 ATAGTGTCAC CTAAATAGGC CTAATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 241 CTCCGCGGCC TAGGCTAGAG TCCGGAGGCT GGATCGGTCC CGGTGTCTTC TATGGAGGTC 301 AAAACAGCGT GGATGGCGTC TCCAGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTTAT 361 ATAGACCTCC CACCGTACAC GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG 421 ACATTTTGGA AAGTCCCGTT GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG 481 GTGGAGACTT GGAAATCCCC GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCA 541 AAACCGCATC ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA 601 GTCCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC 661 AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG 721 TAAATACTCC ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA 781 CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC 841 CGTAAGTTAT GTAACGACAT GCATCTAATG AGTGAAAGGG CCTCGTACTA CGCCTATTTT 901 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA 961 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA 1021 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AACGCGCGAA TTGCAAGCTC 1081 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 1141 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 1201 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 1261 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 1321 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 1381 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 1441 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 1501 CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 1561 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 1621 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 1681 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 1741 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 1801 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 1861 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 1921 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC 1981 CATAACTTCG TATAGCATAC ATTATACGAA GTTATGGCAT GAGATTATCA AAAAGGATCT 2041 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 2101 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 2161 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 2221 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 2281 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 2341 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 2401 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 2461 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 2521 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 2581 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 2641 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 2701 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 2761 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 2821 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 2881 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 2941 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 3001 GAAGCATTTA TCAGGGTTAT TGTCTCATGC CAGGGGTGGG CACACATATT TGATACCAGC 3061 GATCCCTACA CAGCACATAA TTCAATGCGA CTTCCCTCTA TCGCACATCT TAGACCTTTA 3121 TTCTCCCTCC AGCACACATC GAAGCTGCCG AGCAAGCCGT TCTCACCAGT CCAAGACCTG 3181 GCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 3241 CATTTCCCCG AAAAGTGCCA CCTGAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT 3301 TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT 3361 ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC 3421 CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG 3481 GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC 3541 TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG 3601 TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG 3661 CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT 3721 CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC 3781 TATTACGCCA GCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA 3841 GGATCGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC 3901 AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA 3961 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG 4021 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT 4081 ATGATCATGA ACAGACTGTG AGGACTGAGG GGCCTGAAAT GAGCCTTGGG ACTGTGAATC 4141 TAAAATACAC AAACAATTAG AATCACTAGC TCCTGTGTAT AATATTTTCA TAAATCATAC 4201 TCAGTAAGCA AAACTCTCAA GCAGCAAGCA TATGCAGCTA GTTTAACACA TTATACACTT 4261 AAAAATTTTA TATTTACCTT AGAGCTTTAA ATCTCTGTAG GTAGTTTGTC CAATTATGTC 4321 ACACCACAGA AGTAAGGTTC CTTCACAAAG ATCCCAAGCT AGCAGTTTTC CCAGTCACGA 4381 CGTTGTAAAA CGACGG // 0pCMV-Sport 6 "Times New RomanYC0"T7_pro"Times New RomanYCɆ0!M13_forward20_pri"Times New RomanYC0$<attB2"Times New RomanYCɆ0attB1"Times New RomanY0Sp6_pri"Times New RomanYC0M13_pUC_rev_pri"Times New RomanYC0 CMV2_pro"Times New RomanYCɆ0H`LNCX_pri"Times New RomanYC0HCMV_pro"Times New Roman*Y0Ob pCEP_fwd_pri"Times New Roman?YCɆ0_WCMV_immearly_pro"Times New RomanTYC0z CMV_fwd_pri"Times New RomanY04SCAG_enh"Times New RomanYC0AmpR_pro"Times New RomanYC0 pBR322_origin"Times New RomanYCɆ0loxP"Times New RomanY0E ORF 2"Times New RomanYC0E  Ampicillin"Times New RomanYC0E w f1_origin"Times New RomanY0 SV40_PA_term"Times New RomanYCɆ01 EBV_rev_pri"Times New RomanYC0SV40_int"Times New RomanYC0(M13_pUC_fwd_pri"Times New Roman Y͊HindIII(67),ApaI(77),XhoI(79),XbaI(91),NotI(98),EagI(98),SpeI(107),SalI(119),EcoRV(127),SmaI(132),EcoRI(135),AgeI(144),KpnI(151),StuI(199)"Times New RomanC+$059 YSacII"Times New Roman0 YNcoI"Times New Roman(0 #11k YClaI(3844),HpaI(3980)"Times New Roman0m YBclI"Times New Roman0  YNheI"Times New Roman0ba_? Ytest"Times New Roman0 m Ytest"Times New Roman00`