Sequence of pCMV-Tag3

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pCMV-Tag3

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pCMV-Tag3 : 4326 BP; 1056 A; 1116 C; 1107 G; 1047 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 60

1 tacgtaatca ataattatca ttagttaatg ccccagtaat caagtatcgg gtatatacct 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 120

61 caaggcgcaa tgtattgaat gccatttacc gggcggaccg actggcgggt tgctgggggc 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 180

121 gggtaactgc agttattact gcatacaagg gtatcattgc ggttatccct gaaaggtaac 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(240)

181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 240

181 tgcagttacc cacctcataa atgccatttg acgggtgaac cgtcatgtag ttcacatagt 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 300

241 atacggttca tgcgggggat aactgcagtt actgccattt accgggcgga ccgtaatacg 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 360

301 ggtcatgtac tggaataccc tgaaaggatg aaccgtcatg tagatgcata atcagtagcg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 420

361 ataatggtac cactacgcca aaaccgtcat gtagttaccc gcacctatcg ccaaactgag 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 480

421 tgcccctaaa ggttcagagg tggggtaact gcagttaccc tcaaacaaaa ccgtggtttt 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 540

481 agttgccctg aaaggtttta cagcattgtt gaggcggggt aactgcgttt acccgccatc 540

.......... ....... CMV_immearly_pro ....... ..... CMV_fwd_pri CMV_pro

.......... .......... .......... .......... .......... ..........

541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 600

541 cgcacatgcc accctccaga tatattcgtc tcgaccaaat cacttggcag tctaggcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc 660

601 cgctaatgcg gttcgagctt taattgggag tgatttccct tgttttcgac ctcgaggtgg 660

.......... .......... T3_pro .......... .......... ..........

SacII(662) NotI(668) .......... .......... .......... ..........

661 gcggtggcgg ccgccatgga gcagaaactc atctctgaag aggatctggc ccgggcggat 720

661 cgccaccgcc ggcggtacct cgtctttgag tagagacttc tcctagaccg ggcccgccta 720

.......... .......... .......... .......... .......... ..........

PstI,EcoRI(735) EcoRV(743) HindIII(747) SalI(762) ......

721 cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc 780

721 gggggcccga cgtccttaag ctatagttcg aatagctatg gcagctggag ctcccccccg 780

.......... .......... .......... ...... pBluescriptKS_pri ......

ApaI(781) .......... .......... .......... .......... ..........

781 ccggtacctt aattaattaa ggtaccaggt aagtgtaccc aattcgccct atagtgagtc 840

781 ggccatggaa ttaattaatt ccatggtcca ttcacatggg ttaagcggga tatcactcag 840

.......... .......... .......... .......... .......... T7_pro

.......... .......... .......... .......... .......... ..........

841 gtattacaat tcactcgatc gcccttccca acagttgcgc agcctgaatg gcgaatggag 900

841 cataatgtta agtgagctag cgggaagggt tgtcaacgcg tcggacttac cgcttacctc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atccaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag 960

901 taggttaaaa attcacatat tacacaattt gatgactaag attaacaaac acataaaatc 960

.......... .......... ........ SV40_3_splice ........ ..........

.......... .......... .......... .......... .......... ..........

961 attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata 1020

961 taagtgtcag ggttccgagt aaagtccggg gagtcaggag tgtcagacaa gtactagtat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 1080

1021 tagtcggtat ggtgtaaaca tctccaaaat gaacgaaatt ttttggaggg tgtggagggg 1080

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... HpaI(1119) .......... ..........

1081 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 1140

1081 gacttggact ttgtatttta cttacgttaa caacaacaat tgaacaaata acgtcgaata 1140

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 1200

1141 ttaccaatgt ttatttcgtt atcgtagtgt ttaaagtgtt tatttcgtaa aaaaagtgac 1200

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt 1260

1201 gtaagatcaa caccaaacag gtttgagtag ttacatagaa ttgcgcattt aacattcgca 1260

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata 1320

1261 attataaaac aattttaagc gcaatttaaa aacaatttag tcgagtaaaa aattggttat 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 1380

1321 ccggctttag ccgttttagg gaatatttag ttttcttatc tggctctatc ccaactcaca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 1440

1381 acaaggtcaa accttgttct caggtgataa tttcttgcac ctgaggttgc agtttcccgc 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 1500

1441 tttttggcag atagtcccgc taccgggtga tgcacttggt agtgggatta gttcaaaaaa 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 1560

1501 ccccagctcc acggcatttc gtgatttagc cttgggattt ccctcggggg ctaaatctcg 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 1620

1561 aactgcccct ttcggccgct tgcaccgctc tttccttccc ttctttcgct ttcctcgccc 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 1680

1621 gcgatcccgc gaccgttcac atcgccagtg cgacgcgcat tggtggtgtg ggcggcgcga 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 1740

1681 attacgcggc gatgtcccgc gcagtccacc gtgaaaagcc cctttacacg cgccttgggg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 1800

1741 ataaacaaat aaaaagattt atgtaagttt atacataggc gagtactctg ttattgggac 1800

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ataaatgctt caataatatt gaaaaaggaa gaatcctgag gcggaaagaa ccagctgtgg 1860

1801 tatttacgaa gttattataa ctttttcctt cttaggactc cgcctttctt ggtcgacacc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 1920

1861 ttacacacag tcaatcccac acctttcagg ggtccgaggg gtcgtccgtc ttcatacgtt 1920

pBABE_3_pri ........ ...... SV40_enh SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

1921 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 1980

1921 tcgtacgtag agttaatcag tcgttggtcc acacctttca ggggtccgag gggtcgtccg 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 2040

1981 tcttcatacg tttcgtacgt agagttaatc agtcgttggt atcagggcgg ggattgaggc 2040

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 2100

2041 gggtagggcg gggattgagg cgggtcaagg cgggtaagag gcggggtacc gactgattaa 2100

......... SV40_enh SV40_pro SV40_origin ......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 2160

2101 aaaaaataaa tacgtctccg gctccggcgg agccggagac tcgataaggt cttcatcact 2160

.. SV40_origin SV40pro_F_pri SV40_pro .. .......... ..........

.......... StuI(2177) .......... .......... .......... ..........

2161 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc 2220

2161 cctccgaaaa aacctccgga tccgaaaacg tttctagcta gttctctgtc ctactcctag 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 2280

2221 caaagcgtac taacttgttc tacctaacgt gcgtccaaga ggccggcgaa cccacctctc 2280

.......... .......... ... ORF 2 Neomycin(variant) ... ..........

.......... .......... .......... .......... .......... ..........

2281 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 2340

2281 cgataagccg atactgaccc gtgttgtctg ttagccgacg agactacggc ggcacaaggc 2340

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 2400

2341 cgacagtcgc gtccccgcgg gccaagaaaa acagttctgg ctggacaggc cacgggactt 2400

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... MscI(2438) .......... ..........

2401 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 2460

2401 acttgacgtt ctgctccgtc gcgccgatag caccgaccgg tgctgcccgc aaggaacgcg 2460

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 2520

2461 tcgacacgag ctgcaacagt gacttcgccc ttccctgacc gacgataacc cgcttcacgg 2520

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 2580

2521 ccccgtccta gaggacagta gagtggaacg aggacggctc tttcataggt agtaccgact 2580

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 2640

2581 acgttacgcc gccgacgtat gcgaactagg ccgatggacg ggtaagctgg tggttcgctt 2640

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 2700

2641 tgtagcgtag ctcgctcgtg catgagccta ccttcggcca gaacagctag tcctactaga 2700

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggacgaagaa catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat 2760

2701 cctgcttctt gtagtccccg agcgcggtcg gcttgacaag cggtccgagt tccgctcgta 2760

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 2820

2761 cgggctgccg ctcctagagc agcactgggt accgctacgg acgaacggct tatagtacca 2820

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 2880

2821 ccttttaccg gcgaaaagac ctaagtagct gacaccggcc gacccacacc gcctggcgat 2880

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcaggacata gcgttggcta cccgtgatat tgctgaagaa cttggcggcg aatgggctga 2940

2881 agtcctgtat cgcaaccgat gggcactata acgacttctt gaaccgccgc ttacccgact 2940

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 3000

2941 ggcgaaggag cacgaaatgc catagcggcg agggctaagc gtcgcgtagc ggaagatagc 3000

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... ......... BstBI(3038) ......... ..........

3001 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg 3060

3001 ggaagaactg ctcaagaaga ctcgccctga gaccccaagc tttactggct ggttcgctgc 3060

Neomycin(variant) ORF 2 .. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 3120

3061 gggttggacg gtagtgctct aaagctaagg tggcggcgga agatactttc caacccgaag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 3180

3121 ccttagcaaa aggccctgcg gccgacctac taggaggtcg cgcccctaga gtacgacctc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga 3240

3181 aagaagcggg tgggatcccc ctccgattga ctttgtgcct tcctctgtta tggccttcct 3240

.......... .......... .......... TK_PA_term .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt 3300

3241 tgggcgcgat actgccgtta tttttctgtc ttattttgcg tgccacaacc cagcaaacaa 3300

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat 3360

3301 gtatttgcgc cccaagccag ggtcccgacc gtgagacagc tatggggtgg ctctggggta 3360

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa 3420

3361 accccggtta tgcgggcgca aagaaggaaa aggggtgggg tggggggttc aagcccactt 3420

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca 3480

3421 ccgggtcccg agcgtcggtt gcagccccgc cgtccgggac ggtatcggag tccaatgagt 3480

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 3540

3481 atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3600

3541 gaaaaactat tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3660

3601 ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg 3660

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3720

3661 acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 3780

3721 ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3840

3781 gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3900

3841 cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... ......... ApaLI(3960)

3901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3960

3901 aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 4020

3961 acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 4080

4021 gatactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 4140

4081 tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 4200

4141 tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 4260

4201 cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg 4260

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 4320

4261 accggaaaac gagtgtacaa gaaaggacgc aataggggac taagacacct attggcataa 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 accgcc.... .......... .......... .......... .......... .......... 4326

4321 tggcgg.... .......... .......... .......... .......... .......... 4326

.......... .......... .......... .......... .......... ..........