Sequence of pCMV-Tag4

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Sequence of pCMV-Tag4

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Sequence of pCMV-Tag4 : 4319 BP; 1057 A; 1113 C; 1102 G; 1047 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 60

1 tacgtaatca ataattatca ttagttaatg ccccagtaat caagtatcgg gtatatacct 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 120

61 caaggcgcaa tgtattgaat gccatttacc gggcggaccg actggcgggt tgctgggggc 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 180

121 gggtaactgc agttattact gcatacaagg gtatcattgc ggttatccct gaaaggtaac 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(240)

181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 240

181 tgcagttacc cacctcataa atgccatttg acgggtgaac cgtcatgtag ttcacatagt 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 300

241 atacggttca tgcgggggat aactgcagtt actgccattt accgggcgga ccgtaatacg 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 360

301 ggtcatgtac tggaataccc tgaaaggatg aaccgtcatg tagatgcata atcagtagcg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 420

361 ataatggtac cactacgcca aaaccgtcat gtagttaccc gcacctatcg ccaaactgag 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 480

421 tgcccctaaa ggttcagagg tggggtaact gcagttaccc tcaaacaaaa ccgtggtttt 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 540

481 agttgccctg aaaggtttta cagcattgtt gaggcggggt aactgcgttt acccgccatc 540

.......... ....... CMV_immearly_pro ....... ..... CMV_fwd_pri CMV_pro

.......... .......... .......... .......... .......... ..........

541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 600

541 cgcacatgcc accctccaga tatattcgtc tcgaccaaat cacttggcag tctaggcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc 660

601 cgctaatgcg gttcgagctt taattgggag tgatttccct tgttttcgac ctcgaggtgg 660

.......... .......... T3_pro .......... .......... ..........

SacII2) NotI(668) BamHI(687) ..... PstI(703),EcoRI(705) .....

661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc 720

661 cgccaccgcc ggcgagatcg ggcccgccta gggggcccga cgtccttaag ctatagttcg 720

.......... .......... .......... .......... .......... ..........

..... SalI(732) XhoI(738) ..... .......... .......... ApaI(774)

721 ttatcgatac cgtcgacctc gaggattaca aggatgacga cgataagtag ggcccggtac 780

721 aatagctatg gcagctggag ctcctaatgt tcctactgct gctattcatc ccgggccatg 780

...... pBluescriptKS_pri ...... FLAG .......... ..........

.......... .......... .......... .......... .......... ..........

781 cttaattaat taaggtacca ggtaagtgta cccaattcgc cctatagtga gtcgtattac 840

781 gaattaatta attccatggt ccattcacat gggttaagcg ggatatcact cagcataatg 840

.......... .......... .......... .......... T7_pro ..........

.......... .......... .......... .......... .......... ..........

841 aattcactcg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gagatccaat 900

841 ttaagtgagc tagcgggaag ggttgtcaac gcgtcggact taccgcttac ctctaggtta 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattcaca 960

901 aaaattcaca tattacacaa tttgatgact aagattaaca aacacataaa atctaagtgt 960

.......... ........ SV40_3_splice ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc 1020

961 cagggttccg agtaaagtcc ggggagtcag gagtgtcaga caagtactag tattagtcgg 1020

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

1021 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 1080

1021 tatggtgtaa acatctccaa aatgaacgaa attttttgga gggtgtggag ggggacttgg 1080

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... HpaI(1112) .......... ..........

1081 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 1140

1081 actttgtatt ttacttacgt taacaacaac aattgaacaa ataacgtcga atattaccaa 1140

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 1200

1141 tgtttatttc gttatcgtag tgtttaaagt gtttatttcg taaaaaaagt gacgtaagat 1200

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt 1260

1201 caacaccaaa caggtttgag tagttacata gaattgcgca tttaacattc gcaattataa 1260

... SV40_PA_term EBV_rev_pri ... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 1320

1261 aacaatttta agcgcaattt aaaaacaatt tagtcgagta aaaaattggt tatccggctt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 1380

1321 tagccgtttt agggaatatt tagttttctt atctggctct atcccaactc acaacaaggt 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 1440

1381 caaaccttgt tctcaggtga taatttcttg cacctgaggt tgcagtttcc cgctttttgg 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 1500

1441 cagatagtcc cgctaccggg tgatgcactt ggtagtggga ttagttcaaa aaaccccagc 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 1560

1501 tccacggcat ttcgtgattt agccttggga tttccctcgg gggctaaatc tcgaactgcc 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 1620

1561 cctttcggcc gcttgcaccg ctctttcctt cccttctttc gctttcctcg cccgcgatcc 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 1680

1621 cgcgaccgtt cacatcgcca gtgcgacgcg cattggtggt gtgggcggcg cgaattacgc 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 1740

1681 ggcgatgtcc cgcgcagtcc accgtgaaaa gcccctttac acgcgccttg gggataaaca 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 1800

1741 aataaaaaga tttatgtaag tttatacata ggcgagtact ctgttattgg gactatttac 1800

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cttcaataat attgaaaaag gaagaatcct gaggcggaaa gaaccagctg tggaatgtgt 1860

1801 gaagttatta taactttttc cttcttagga ctccgccttt cttggtcgac accttacaca 1860

.......... .......... .......... .......... ......... pBABE_3_pri

.......... .......... .......... .......... .......... ..........

1861 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 1920

1861 cagtcaatcc cacacctttc aggggtccga ggggtcgtcc gtcttcatac gtttcgtacg 1920

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 1980

1921 tagagttaat cagtcgttgg tccacacctt tcaggggtcc gaggggtcgt ccgtcttcat 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 2040

1981 acgtttcgta cgtagagtta atcagtcgtt ggtatcaggg cggggattga ggcgggtagg 2040

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 2100

2041 gcggggattg aggcgggtca aggcgggtaa gaggcggggt accgactgat taaaaaaaat 2100

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 2160

2101 aaatacgtct ccggctccgg cggagccgga gactcgataa ggtcttcatc actcctccga 2160

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

StuI(2170) .......... .......... .......... .......... ..........

2161 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc 2220

2161 aaaaacctcc ggatccgaaa acgtttctag ctagttctct gtcctactcc tagcaaagcg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 2280

2221 tactaacttg ttctacctaa cgtgcgtcca agaggccggc gaacccacct ctccgataag 2280

.......... ...... ORF 1 Neomycin(variant) ...... ..........

.......... .......... .......... .......... .......... ..........

2281 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 2340

2281 ccgatactga cccgtgttgt ctgttagccg acgagactac ggcggcacaa ggccgacagt 2340

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 2400

2341 cgcgtccccg cgggccaaga aaaacagttc tggctggaca ggccacggga cttacttgac 2400

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... MscI(2431) .......... ..........

2401 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 2460

2401 gttctgctcc gtcgcgccga tagcaccgac cggtgctgcc cgcaaggaac gcgtcgacac 2460

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 2520

2461 gagctgcaac agtgacttcg cccttccctg accgacgata acccgcttca cggccccgtc 2520

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 2580

2521 ctagaggaca gtagagtgga acgaggacgg ctctttcata ggtagtaccg actacgttac 2580

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 2640

2581 gccgccgacg tatgcgaact aggccgatgg acgggtaagc tggtggttcg ctttgtagcg 2640

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 2700

2641 tagctcgctc gtgcatgagc ctaccttcgg ccagaacagc tagtcctact agacctgctt 2700

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaacatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 2760

2701 cttgtagtcc ccgagcgcgg tcggcttgac aagcggtccg agttccgctc gtacgggctg 2760

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 2820

2761 ccgctcctag agcagcactg ggtaccgcta cggacgaacg gcttatagta ccacctttta 2820

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 2880

2821 ccggcgaaaa gacctaagta gctgacaccg gccgacccac accgcctggc gatagtcctg 2880

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atagcgttgg ctacccgtga tattgctgaa gaacttggcg gcgaatgggc tgaccgcttc 2940

2881 tatcgcaacc gatgggcact ataacgactt cttgaaccgc cgcttacccg actggcgaag 2940

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 3000

2941 gagcacgaaa tgccatagcg gcgagggcta agcgtcgcgt agcggaagat agcggaagaa 3000

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... ......... BstBI(3031) ......... ..........

3001 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 3060

3001 ctgctcaaga agactcgccc tgagacccca agctttactg gctggttcgc tgcgggttgg 3060

ORF 1 Neomycin(variant) ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 3120

3061 acggtagtgc tctaaagcta aggtggcggc ggaagatact ttccaacccg aagccttagc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 3180

3121 aaaaggccct gcggccgacc tactaggagg tcgcgcccct agagtacgac ctcaagaagc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg 3240

3181 gggtgggatc cccctccgat tgactttgtg ccttcctctg ttatggcctt ccttgggcgc 3240

.......... .......... .......... TK_PA_term .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac 3300

3241 gatactgccg ttatttttct gtcttatttt gcgtgccaca acccagcaaa caagtatttg 3300

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 3360

3301 cgccccaagc cagggtcccg accgtgagac agctatgggg tggctctggg gtaaccccgg 3360

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 3420

3361 ttatgcgggc gcaaagaagg aaaaggggtg gggtgggggg ttcaagccca cttccgggtc 3420

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac 3480

3421 ccgagcgtcg gttgcagccc cgccgtccgg gacggtatcg gagtccaatg agtatatatg 3480

.......... TK_PA_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 3540

3481 aaatctaact aaattttgaa gtaaaaatta aattttccta gatccacttc taggaaaaac 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 3600

3541 tattagagta ctggttttag ggaattgcac tcaaaagcaa ggtgactcgc agtctggggc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 3660

3601 atcttttcta gtttcctaga agaactctag gaaaaaaaga cgcgcattag acgacgaacg 3660

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 3720

3661 tttgtttttt tggtggcgat ggtcgccacc aaacaaacgg cctagttctc gatggttgag 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 3780

3721 aaaaaggctt ccattgaccg aagtcgtctc gcgtctatgg tttatgacag gaagatcaca 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 3840

3781 tcggcatcaa tccggtggtg aagttcttga gacatcgtgg cggatgtatg gagcgagacg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 3900

3841 attaggacaa tggtcaccga cgacggtcac cgctattcag cacagaatgg cccaacctga 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... ......... ApaLI(3953)

3901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 3960

3901 gttctgctat caatggccta ttccgcgtcg ccagcccgac ttgcccccca agcacgtgtg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 4020

3961 tcgggtcgaa cctcgcttgc tggatgtggc ttgactctat ggatgtcgca ctcgatactc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 4080

4021 tttcgcggtg cgaagggctt ccctctttcc gcctgtccat aggccattcg ccgtcccagc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 4140

4081 cttgtcctct cgcgtgctcc ctcgaaggtc cccctttgcg gaccatagaa atatcaggac 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 4200

4141 agcccaaagc ggtggagact gaactcgcag ctaaaaacac tacgagcagt ccccccgcct 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 4260

4201 cggatacctt tttgcggtcg ttgcgccgga aaaatgccaa ggaccggaaa acgaccggaa 4260

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcc. 4319

4261 aacgagtgta caagaaagga cgcaataggg gactaagaca cctattggca taatggcgg. 4319

.......... .......... .......... .......... .......... ..........