KTKTLOCUS pCMV-Tag5 4322 bp DNA SYN 28-Aug-2007 DEFINITION pCMV-Tag5 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4322 /organism="pCMV-Tag5" /mol_type="other DNA" misc_feature 71..358 /label="CAG_enhancer" promoter 16..568 /label="CMV_immearly_promoter" misc_feature 525..545 /label="CMV_fwd_primer" promoter 526..595 /label="CMV_promoter" promoter 620..639 /label="T3_promoter" misc_feature complement(726..742) /label="pBluescriptKS_primer" promoter complement(824..842) /label="T7_promoter" misc_feature 899..958 /label="SV40_3_splice" terminator 1009..1237 /label="SV40_PA_terminator" misc_feature 1207..1226 /label="EBV_rev_primer" rep_origin complement(1376..1682) /label="f1_origin" promoter 1761..1789 /label="AmpR_promoter" misc_feature complement(1855..1875) /label="pBABE_3_primer" misc_feature complement(1861..2076) /label="SV40_enhancer" promoter 1873..2141 /label="SV40_promoter" rep_origin 2040..2117 /label="SV40_origin" misc_feature 2102..2121 /label="SV40pro_F_primer" CDS 2224..3018 /label="Neomycin(variant)" /gene="Neomycin(variant)" /note="ORF frame 1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 2227..3015 /label="Neomycin(variant)" /gene="Neomycin(variant)" terminator 3193..3462 /label="TK_PA_terminator" rep_origin 3610..4229 /label="pBR322_origin" ORIGIN 1 ATGCATTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA 61 GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG 121 CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG 181 ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA 241 TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC 301 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC 361 TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC 421 ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA 481 TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG 541 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA 601 GCGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTCCACC 661 GCGGTGGCGG CCGCTCTAGC CCGGGCGGAT CCCCCGGGCT GCAGGAATTC GATATCAAGC 721 TTATCGATAC CGTCGACCTC GAGCAGAAAC TCATCTCTGA AGAGGATCTG TAGGGCCCGG 781 TACCTTAATT AATTAAGGTA CCAGGTAAGT GTACCCAATT CGCCCTATAG TGAGTCGTAT 841 TACAATTCAC TCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGAGATCC 901 AATTTTTAAG TGTATAATGT GTTAAACTAC TGATTCTAAT TGTTTGTGTA TTTTAGATTC 961 ACAGTCCCAA GGCTCATTTC AGGCCCCTCA GTCCTCACAG TCTGTTCATG ATCATAATCA 1021 GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA 1081 ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG 1141 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 1201 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAACG CGTAAATTGT AAGCGTTAAT 1261 ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC 1321 GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT 1381 CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA 1441 ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG 1501 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA 1561 CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT 1621 AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT 1681 GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 1741 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 1801 ATGCTTCAAT AATATTGAAA AAGGAAGAAT CCTGAGGCGG AAAGAACCAG CTGTGGAATG 1861 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 1921 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 1981 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 2041 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT 2101 TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 2161 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATCGTTT 2221 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 2281 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 2341 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 2401 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 2461 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 2521 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 2581 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 2641 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 2701 GAAGAACATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC 2761 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 2821 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG 2881 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAACTTG GCGGCGAATG GGCTGACCGC 2941 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 3001 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 3061 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 3121 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 3181 TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC 3241 GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG TTTGTTCATA 3301 AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG 3361 GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC 3421 CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCCTCAGGT TACTCATATA 3481 TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT 3541 TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC 3601 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 3661 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA 3721 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG 3781 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC 3841 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG 3901 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 3961 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT 4021 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG 4081 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC 4141 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC 4201 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 4261 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG 4321 CC //  pCMV-Tag5 "Times New RomanKTCGfCAG_enh"Times New RomanKTC8CMV_immearly_pro"Times New RomanKTCɆ ! CMV_fwd_pri"Times New RomanKTSCMV_pro"Times New Roman)KTClT3_pro"Times New RomanKTCpBluescriptKS_pri"Times New RomanKTCɆ8JT7_pro"Times New RomanKT SV40_3_splice"Times New RomanKTC SV40_PA_term"Times New RomanKTC EBV_rev_pri"Times New RomanKTCɆ` f1_origin"Times New RomanKTAmpR_pro"Times New RomanKTC?S pBABE_3_pri"Times New RomanKTCESV40_enh"Times New RomanKTCɆQ]SV40_pro"Times New Roman(KTE SV40_origin"Times New RomanKTC6I SV40pro_F_pri"Times New RomanKTC ORF 1"Times New RomanKTCɆ Neomycin(variant)"Times New RomanKTy  TK_PA_term"Times New RomanKTC pBR322_origin"Times New Roman KTNdeI"Times New Romanpap KTtNheI(597),SacI(655),SacII(662),NotI(668),BamHI(687),PstI(703),EcoRI(705),EcoRV(713),HindIII(717),SalI(732),XhoI(738)"Times New RomanU!00y KTApaI"Times New Roman 5 GYY KTBclI(1009),HpaI(1115)"Times New RomanIU4X$dd$ KTStuI"Times New Roman}`S`g_zAz} KTNarI(2352),MscI(2434)"Times New Roman0 %K ^pp KTBstBI"Times New Roman t`` KTApaLI"Times New Romantr KTtest"Times New Roman4\%%SP KTtest"Times New RomanH[jjX