Sequence of pCMV-Tag5

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Sequence of pCMV-Tag5

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Sequence of pCMV-Tag5 : 4322 BP; 1056 A; 1116 C; 1101 G; 1049 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 60

1 tacgtaatca ataattatca ttagttaatg ccccagtaat caagtatcgg gtatatacct 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 120

61 caaggcgcaa tgtattgaat gccatttacc gggcggaccg actggcgggt tgctgggggc 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 180

121 gggtaactgc agttattact gcatacaagg gtatcattgc ggttatccct gaaaggtaac 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(240)

181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 240

181 tgcagttacc cacctcataa atgccatttg acgggtgaac cgtcatgtag ttcacatagt 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 300

241 atacggttca tgcgggggat aactgcagtt actgccattt accgggcgga ccgtaatacg 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 360

301 ggtcatgtac tggaataccc tgaaaggatg aaccgtcatg tagatgcata atcagtagcg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 420

361 ataatggtac cactacgcca aaaccgtcat gtagttaccc gcacctatcg ccaaactgag 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 480

421 tgcccctaaa ggttcagagg tggggtaact gcagttaccc tcaaacaaaa ccgtggtttt 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 540

481 agttgccctg aaaggtttta cagcattgtt gaggcggggt aactgcgttt acccgccatc 540

.......... ....... CMV_immearly_pro ....... ..... CMV_fwd_pri CMV_pro

.......... .......... .......... .......... .......... ..........

541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 600

541 cgcacatgcc accctccaga tatattcgtc tcgaccaaat cacttggcag tctaggcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc 660

601 cgctaatgcg gttcgagctt taattgggag tgatttccct tgttttcgac ctcgaggtgg 660

.......... .......... T3_pro .......... .......... ..........

SacII2) NotI(668) BamHI(687) ..... PstI(703),EcoRI(705) .....

661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc 720

661 cgccaccgcc ggcgagatcg ggcccgccta gggggcccga cgtccttaag ctatagttcg 720

.......... .......... .......... .......... .......... ..........

..... SalI(732) XhoI(738) ..... .......... .......... ApaI(777)

721 ttatcgatac cgtcgacctc gagcagaaac tcatctctga agaggatctg tagggcccgg 780

721 aatagctatg gcagctggag ctcgtctttg agtagagact tctcctagac atcccgggcc 780

...... pBluescriptKS_pri ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 taccttaatt aattaaggta ccaggtaagt gtacccaatt cgccctatag tgagtcgtat 840

781 atggaattaa ttaattccat ggtccattca catgggttaa gcgggatatc actcagcata 840

.......... .......... .......... .......... .......... T7_pro

.......... .......... .......... .......... .......... ..........

841 tacaattcac tcgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggagatcc 900

841 atgttaagtg agctagcggg aagggttgtc aacgcgtcgg acttaccgct tacctctagg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc 960

901 ttaaaaattc acatattaca caatttgatg actaagatta acaaacacat aaaatctaag 960

.......... ........ SV40_3_splice ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 acagtcccaa ggctcatttc aggcccctca gtcctcacag tctgttcatg atcataatca 1020

961 tgtcagggtt ccgagtaaag tccggggagt caggagtgtc agacaagtac tagtattagt 1020

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

1021 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 1080

1021 cggtatggtg taaacatctc caaaatgaac gaaatttttt ggagggtgtg gagggggact 1080

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... HpaI(1115) .......... ..........

1081 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 1140

1081 tggactttgt attttactta cgttaacaac aacaattgaa caaataacgt cgaatattac 1140

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 1200

1141 caatgtttat ttcgttatcg tagtgtttaa agtgtttatt tcgtaaaaaa agtgacgtaa 1200

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat 1260

1201 gatcaacacc aaacaggttt gagtagttac atagaattgc gcatttaaca ttcgcaatta 1260

... SV40_PA_term EBV_rev_pri ... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 1320

1261 taaaacaatt ttaagcgcaa tttaaaaaca atttagtcga gtaaaaaatt ggttatccgg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 1380

1321 ctttagccgt tttagggaat atttagtttt cttatctggc tctatcccaa ctcacaacaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 1440

1381 ggtcaaacct tgttctcagg tgataatttc ttgcacctga ggttgcagtt tcccgctttt 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 1500

1441 tggcagatag tcccgctacc gggtgatgca cttggtagtg ggattagttc aaaaaacccc 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 1560

1501 agctccacgg catttcgtga tttagccttg ggatttccct cgggggctaa atctcgaact 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 1620

1561 gcccctttcg gccgcttgca ccgctctttc cttcccttct ttcgctttcc tcgcccgcga 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 1680

1621 tcccgcgacc gttcacatcg ccagtgcgac gcgcattggt ggtgtgggcg gcgcgaatta 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 1740

1681 cgcggcgatg tcccgcgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 1800

1741 acaaataaaa agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt 1800

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atgcttcaat aatattgaaa aaggaagaat cctgaggcgg aaagaaccag ctgtggaatg 1860

1801 tacgaagtta ttataacttt ttccttctta ggactccgcc tttcttggtc gacaccttac 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 1920

1861 acacagtcaa tcccacacct ttcaggggtc cgaggggtcg tccgtcttca tacgtttcgt 1920

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 1980

1921 acgtagagtt aatcagtcgt tggtccacac ctttcagggg tccgaggggt cgtccgtctt 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 2040

1981 catacgtttc gtacgtagag ttaatcagtc gttggtatca gggcggggat tgaggcgggt 2040

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 2100

2041 agggcgggga ttgaggcggg tcaaggcggg taagaggcgg ggtaccgact gattaaaaaa 2100

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 2160

2101 aataaatacg tctccggctc cggcggagcc ggagactcga taaggtcttc atcactcctc 2160

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... StuI(2173) .......... .......... .......... ..........

2161 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt 2220

2161 cgaaaaaacc tccggatccg aaaacgtttc tagctagttc tctgtcctac tcctagcaaa 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 2280

2221 gcgtactaac ttgttctacc taacgtgcgt ccaagaggcc ggcgaaccca cctctccgat 2280

.......... .......... ... ORF 1 Neomycin(variant) ... ..........

.......... .......... .......... .......... .......... ..........

2281 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 2340

2281 aagccgatac tgacccgtgt tgtctgttag ccgacgagac tacggcggca caaggccgac 2340

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 2400

2341 agtcgcgtcc ccgcgggcca agaaaaacag ttctggctgg acaggccacg ggacttactt 2400

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... MscI(2434) .......... ..........

2401 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 2460

2401 gacgttctgc tccgtcgcgc cgatagcacc gaccggtgct gcccgcaagg aacgcgtcga 2460

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 2520

2461 cacgagctgc aacagtgact tcgcccttcc ctgaccgacg ataacccgct tcacggcccc 2520

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 2580

2521 gtcctagagg acagtagagt ggaacgagga cggctctttc ataggtagta ccgactacgt 2580

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 2640

2581 tacgccgccg acgtatgcga actaggccga tggacgggta agctggtggt tcgctttgta 2640

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 2700

2641 gcgtagctcg ctcgtgcatg agcctacctt cggccagaac agctagtcct actagacctg 2700

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaagaacatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 2760

2701 cttcttgtag tccccgagcg cggtcggctt gacaagcggt ccgagttccg ctcgtacggg 2760

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 2820

2761 ctgccgctcc tagagcagca ctgggtaccg ctacggacga acggcttata gtaccacctt 2820

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 2880

2821 ttaccggcga aaagacctaa gtagctgaca ccggccgacc cacaccgcct ggcgatagtc 2880

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gacatagcgt tggctacccg tgatattgct gaagaacttg gcggcgaatg ggctgaccgc 2940

2881 ctgtatcgca accgatgggc actataacga cttcttgaac cgccgcttac ccgactggcg 2940

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 3000

2941 aaggagcacg aaatgccata gcggcgaggg ctaagcgtcg cgtagcggaa gatagcggaa 3000

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... ......... BstBI(3034) ......... ..........

3001 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 3060

3001 gaactgctca agaagactcg ccctgagacc ccaagcttta ctggctggtt cgctgcgggt 3060

ORF 1 Neomycin(variant) ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 3120

3061 tggacggtag tgctctaaag ctaaggtggc ggcggaagat actttccaac ccgaagcctt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 3180

3121 agcaaaaggc cctgcggccg acctactagg aggtcgcgcc cctagagtac gacctcaaga 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 3240

3181 agcgggtggg atccccctcc gattgacttt gtgccttcct ctgttatggc cttccttggg 3240

.......... .......... .......... TK_PA_term .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata 3300

3241 cgcgatactg ccgttatttt tctgtcttat tttgcgtgcc acaacccagc aaacaagtat 3300

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 3360

3301 ttgcgcccca agccagggtc ccgaccgtga gacagctatg gggtggctct ggggtaaccc 3360

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 3420

3361 cggttatgcg ggcgcaaaga aggaaaaggg gtggggtggg gggttcaagc ccacttccgg 3420

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata 3480

3421 gtcccgagcg tcggttgcag ccccgccgtc cgggacggta tcggagtcca atgagtatat 3480

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 3540

3481 atgaaatcta actaaatttt gaagtaaaaa ttaaattttc ctagatccac ttctaggaaa 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 3600

3541 aactattaga gtactggttt tagggaattg cactcaaaag caaggtgact cgcagtctgg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 3660

3601 ggcatctttt ctagtttcct agaagaactc taggaaaaaa agacgcgcat tagacgacga 3660

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3661 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 3720

3661 acgtttgttt ttttggtggc gatggtcgcc accaaacaaa cggcctagtt ctcgatggtt 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 3780

3721 gagaaaaagg cttccattga ccgaagtcgt ctcgcgtcta tggtttatga caggaagatc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 3840

3781 acatcggcat caatccggtg gtgaagttct tgagacatcg tggcggatgt atggagcgag 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 3900

3841 acgattagga caatggtcac cgacgacggt caccgctatt cagcacagaa tggcccaacc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... ......... ApaLI(3956)

3901 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 3960

3901 tgagttctgc tatcaatggc ctattccgcg tcgccagccc gacttgcccc ccaagcacgt 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 4020

3961 gtgtcgggtc gaacctcgct tgctggatgt ggcttgactc tatggatgtc gcactcgata 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 4080

4021 ctctttcgcg gtgcgaaggg cttccctctt tccgcctgtc cataggccat tcgccgtccc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 4140

4081 agccttgtcc tctcgcgtgc tccctcgaag gtcccccttt gcggaccata gaaatatcag 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 4200

4141 gacagcccaa agcggtggag actgaactcg cagctaaaaa cactacgagc agtccccccg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 4260

4201 cctcggatac ctttttgcgg tcgttgcgcc ggaaaaatgc caaggaccgg aaaacgaccg 4260

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 4320

4261 gaaaacgagt gtacaagaaa ggacgcaata ggggactaag acacctattg gcataatggc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cc........ .......... .......... .......... .......... .......... 4322

4321 gg........ .......... .......... .......... .......... .......... 4322

.......... .......... .......... .......... .......... ..........