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Gene/insert name: none
Alternative names: pHR' CMV 8.2 deltaR, pCMV-dR8.2 dvpr, pCMVdR8.2 dvpr
Insert size (bp): Unknown
Vector backbone: na (Search Vector Database)
Type of vector: Mammalian expression,Lentiviral
Backbone size (bp): 13457
5' Sequencing primer: CMV Forward (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Bob Weinberg
Comments: Packaging plasmid for Lentiviral constructs (vpr is removed). The 293T cell line can be obtained from the Weinberg lab or GenHunter.
Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA et al. (RNA 2003 Apr;9(4):493-501. Pubmed)