Sequence of pCMV-dR8

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Sequence of pCMV-dR8

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Sequence of pCMV-dR8.2.dvpr : 13457 BP; 4198 A; 2795 C; 3317 G; 3147 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gggctgcagg aattcgagct cgcccgacat tgattattga ctagttatta atagtaatca 60

1 cccgacgtcc ttaagctcga gcgggctgta actaataact gatcaataat tatcattagt 60

.......... .......... .......... ......... CMV_imm_pro .........

.......... .......... .......... .......... .......... ..........

61 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 120

61 taatgcccca gtaatcaagt atcgggtata tacctcaagg cgcaatgtat tgaatgccat 120

.......... ......... CMV_imm_pro ......... ......... CAG_enhancer

.......... .......... .......... .......... .......... ..........

121 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 180

121 ttaccgggcg gaccgactgg cgggttgctg ggggcgggta actgcagtta ttactgcata 180

.......... ... CAG_enhancer CMV_imm_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 240

181 caagggtatc attgcggtta tccctgaaag gtaactgcag ttacccacct cataaatgcc 240

.......... ... CAG_enhancer CMV_imm_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 300

241 atttgacggg tgaaccgtca tgtagttcac atagtatacg gttcatgcgg gggataactg 300

.......... ... CAG_enhancer CMV_imm_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 360

301 cagttactgc catttaccgg gcggaccgta atacgggtca tgtactggaa taccctgaaa 360

.......... ... CAG_enhancer CMV_imm_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg 420

361 ggatgaaccg tcatgtagat gcataatcag tagcgataat ggtaccacta cgccaaaacc 420

........ CAG_enhancer CMV_imm_pro ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc 480

421 gtcatgtagt tacccgcacc tatcgccaaa ctgagtgccc ctaaaggttc agaggtgggg 480

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt 540

481 taactgcagt taccctcaaa caaaaccgtg gttttagttg ccctgaaagg ttttacagca 540

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 600

541 ttgttgaggc ggggtaactg cgtttacccg ccatccgcac atgccaccct ccagatatat 600

CMV_imm_pro CMV_fwd_primer LNCX_primer CMV2_promoter CMV_promoter

.......... .......... .......... .......... .......... ..........

601 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac 660

601 tcgtctcgag caaatcactt ggcagtctag cggacctctg cggtaggtgc gacaaaactg 660

LNCX_primer CMV_promoter pCEP_fwd_primer CMV2_promoter ......

.......... .......... .......... .......... .......... ..........

661 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg 720

661 gaggtatctt ctgtggccct ggctaggtcg gaggcgccgg cccttgccac gtaaccttgc 720

........ CMV2_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc 780

721 gcctaagggg cacggttctc actgcattca tggcggatat ctcagatatc cgggtggggg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttggcttctt atgcgacgga tcgatcccgt aataagcttc gaggtccgcg gccgcgttga 840

781 aaccgaagaa tacgctgcct agctagggca ttattcgaag ctccaggcgc cggcgcaact 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag agcgtcagta 900

841 gcgcgtgccg ttctccgctc cccgccgctg accactctct acccacgctc tcgcagtcat 900

.......... .......... .......... .... ORF1 Gag_HIV(variant) ....

.......... .......... .......... .......... .......... ..........

901 ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg gggaaagaaa 960

901 aattcgcccc ctcttaatct agctaccctt ttttaagcca attccggtcc ccctttcttt 960

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat 1020

961 tttatattta attttgtata tcatacccgt tcgtccctcg atcttgctaa gcgtcaatta 1020

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc 1080

1021 ggaccggaca atctttgtag tcttccgaca tctgtttatg accctgtcga tgttggtagg 1080

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt 1140

1081 gaagtctgtc ctagtcttct tgaatctagt aatatattat gtcatcgttg ggagataaca 1140

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag 1200

1141 cacgtagttt cctatctcta ttttctgtgg ttccttcgaa atctgttcta tctccttctc 1200

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca cagcaatcag 1260

1201 gttttgtttt cattcttttt tcgtgtcgtt cgtcgtcgac tgtgtcctgt gtcgttagtc 1260

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca tcaggccata 1320

1261 cagtcggttt taatgggata tcacgtcttg taggtccccg tttaccatgt agtccggtat 1320

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa 1380

1321 agtggatctt gaaatttacg tacccatttt catcatcttc tcttccgaaa gtcgggtctt 1380

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtgataccca tgttttcagc attatcagaa ggagccaccc cacaagattt aaacaccatg 1440

1381 cactatgggt acaaaagtcg taatagtctt cctcggtggg gtgttctaaa tttgtggtac 1440

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag 1500

1441 gatttgtgtc acccccctgt agttcgtcgg tacgtttaca attttctctg gtagttactc 1500

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc accaggccag 1560

1501 cttcgacgtc ttaccctatc tcacgtaggt cacgtacgtc ccggataacg tggtccggtc 1560

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca ggaacaaata 1620

1561 tactctcttg gttccccttc actgtatcgt ccttgatgat catgggaagt ccttgtttat 1620

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag atggataatc 1680

1621 cctacctact gtgtattagg tggatagggt catcctcttt agatattttc tacctattag 1680

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ctgggattaa ataaaatagt aagaatgtat agccctacca gcattctgga cataagacaa 1740

1681 gaccctaatt tattttatca ttcttacata tcgggatggt cgtaagacct gtattctgtt 1740

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggaccaaagg aaccctttag agactatgta gaccgattct ataaaactct aagagccgag 1800

1741 cctggtttcc ttgggaaatc tctgatacat ctggctaaga tattttgaga ttctcggctc 1800

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 caagcttcac aagaggtaaa aaattggatg acagaaacct tgttggtcca aaatgcgaac 1860

1801 gttcgaagtg ttctccattt tttaacctac tgtctttgga acaaccaggt tttacgcttg 1860

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccagattgta agactatttt aaaagcattg ggaccaggag cgacactaga agaaatgatg 1920

1861 ggtctaacat tctgataaaa ttttcgtaac cctggtcctc gctgtgatct tctttactac 1920

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acagcatgtc agggagtggg gggacccggc cataaagcaa gagttttggc tgaagcaatg 1980

1921 tgtcgtacag tccctcaccc ccctgggccg gtatttcgtt ctcaaaaccg acttcgttac 1980

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agccaagtaa caaatccagc taccataatg atacagaaag gcaattttag gaaccaaaga 2040

1981 tcggttcatt gtttaggtcg atggtattac tatgtctttc cgttaaaatc cttggtttct 2040

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aagactgtta agtgtttcaa ttgtggcaaa gaagggcaca tagccaaaaa ttgcagggcc 2100

2041 ttctgacaat tcacaaagtt aacaccgttt cttcccgtgt atcggttttt aacgtcccgg 2100

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa agattgtact 2160

2101 ggatcctttt tcccgacaac ctttacacct ttccttcctg tggtttactt tctaacatga 2160

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gagagacagg ctaatttttt agggaagatc tggccttccc acaagggaag gccagggaat 2220

2161 ctctctgtcc gattaaaaaa tcccttctag accggaaggg tgttcccttc cggtccctta 2220

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag gtttggggaa 2280

2221 aaagaagtct cgtctggtct cggttgtcgg ggtggtcttc tctcgaagtc caaacccctt 2280

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gagacaacaa ctccctctca gaagcaggag ccgatagaca aggaactgta tcctttagct 2340

2281 ctctgttgtt gagggagagt cttcgtcctc ggctatctgt tccttgacat aggaaatcga 2340

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tccctcagat cactctttgg cagcgacccc tcgtcacaat aaagataggg gggcaattaa 2400

2341 agggagtcta gtgagaaacc gtcgctgggg agcagtgtta tttctatccc cccgttaatt 2400

.......... .... ORF1 Gag_HIV(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aggaagctct attagataca ggagcagatg atacagtatt agaagaaatg aatttgccag 2460

2401 tccttcgaga taatctatgt cctcgtctac tatgtcataa tcttctttac ttaaacggtc 2460

.......... .......... .......... .......... ......... Orf frame 3

.......... .......... .......... .......... .......... BclI(2519)

2461 gaagatggaa accaaaaatg atagggggaa ttggaggttt tatcaaagta ggacagtatg 2520

2461 cttctacctt tggtttttac tatccccctt aacctccaaa atagtttcat cctgtcatac 2520

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atcagatact catagaaatc tgcggacata aagctatagg tacagtatta gtaggaccta 2580

2521 tagtctatga gtatctttag acgcctgtat ttcgatatcc atgtcataat catcctggat 2580

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cacctgtcaa cataattgga agaaatctgt tgactcagat tggctgcact ttaaattttc 2640

2581 gtggacagtt gtattaacct tctttagaca actgagtcta accgacgtga aatttaaaag 2640

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccattagtcc tattgagact gtaccagtaa aattaaagcc aggaatggat ggcccaaaag 2700

2641 ggtaatcagg ataactctga catggtcatt ttaatttcgg tccttaccta ccgggttttc 2700

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt agtagaaatt tgtacagaaa 2760

2701 aatttgttac cggtaactgt cttctttttt attttcgtaa tcatctttaa acatgtcttt 2760

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tggaaaagga aggaaaaatt tcaaaaattg ggcctgaaaa tccatacaat actccagtat 2820

2761 accttttcct tcctttttaa agtttttaac ccggactttt aggtatgtta tgaggtcata 2820

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc agagaactta 2880

2821 aacggtattt cttttttctg tcatgattta cctcttttaa tcatctaaag tctcttgaat 2880

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ataagagaac tcaagatttc tgggaagttc aattaggaat accacatcct gcagggttaa 2940

2881 tattctcttg agttctaaag acccttcaag ttaatcctta tggtgtagga cgtcccaatt 2940

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aacagaaaaa atcagtaaca gtactggatg tgggcgatgc atatttttca gttcccttag 3000

2941 ttgtcttttt tagtcattgt catgacctac acccgctacg tataaaaagt caagggaatc 3000

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ataaagactt caggaagtat actgcattta ccatacctag tataaacaat gagacaccag 3060

3001 tatttctgaa gtccttcata tgacgtaaat ggtatggatc atatttgtta ctctgtggtc 3060

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggattagata tcagtacaat gtgcttccac agggatggaa aggatcacca gcaatattcc 3120

3061 cctaatctat agtcatgtta cacgaaggtg tccctacctt tcctagtggt cgttataagg 3120

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agtgtagcat gacaaaaatc ttagagcctt ttagaaaaca aaatccagac atagtcatct 3180

3121 tcacatcgta ctgtttttag aatctcggaa aatcttttgt tttaggtctg tatcagtaga 3180

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atcaatacat ggatgatttg tatgtaggat ctgacttaga aatagggcag catagaacaa 3240

3181 tagttatgta cctactaaac atacatccta gactgaatct ttatcccgtc gtatcttgtt 3240

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaatagagga actgagacaa catctgttga ggtggggatt taccacacca gacaaaaaac 3300

3241 tttatctcct tgactctgtt gtagacaact ccacccctaa atggtgtggt ctgttttttg 3300

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atcagaaaga acctccattc ctttggatgg gttatgaact ccatcctgat aaatggacag 3360

3301 tagtctttct tggaggtaag gaaacctacc caatacttga ggtaggacta tttacctgtc 3360

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tacagcctat agtgctgcca gaaaaggaca gctggactgt caatgacata cagaaattag 3420

3361 atgtcggata tcacgacggt cttttcctgt cgacctgaca gttactgtat gtctttaatc 3420

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgggaaaatt gaattgggca agtcagattt atgcagggat taaagtaagg caattatgta 3480

3421 acccttttaa cttaacccgt tcagtctaaa tacgtcccta atttcattcc gttaatacat 3480

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aacttcttag gggaaccaaa gcactaacag aagtagtacc actaacagaa gaagcagagc 3540

3481 ttgaagaatc cccttggttt cgtgattgtc ttcatcatgg tgattgtctt cttcgtctcg 3540

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tagaactggc agaaaacagg gagattctaa aagaaccggt acatggagtg tattatgacc 3600

3541 atcttgaccg tcttttgtcc ctctaagatt ttcttggcca tgtacctcac ataatactgg 3600

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 catcaaaaga cttaatagca gaaatacaga agcaggggca aggccaatgg acatatcaaa 3660

3601 gtagttttct gaattatcgt ctttatgtct tcgtccccgt tccggttacc tgtatagttt 3660

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tttatcaaga gccatttaaa aatctgaaaa caggaaaata tgcaagaatg aagggtgccc 3720

3661 aaatagttct cggtaaattt ttagactttt gtccttttat acgttcttac ttcccacggg 3720

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acactaatga tgtgaaacaa ttaacagagg cagtacaaaa aatagccaca gaaagcatag 3780

3721 tgtgattact acactttgtt aattgtctcc gtcatgtttt ttatcggtgt ctttcgtatc 3780

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 taatatgggg aaagactcct aaatttaaat tacccataca aaaggaaaca tgggaagcat 3840

3781 attatacccc tttctgagga tttaaattta atgggtatgt tttcctttgt acccttcgta 3840

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggtggacaga gtattggcaa gccacctgga ttcctgagtg ggagtttgtc aatacccctc 3900

3841 ccacctgtct cataaccgtt cggtggacct aaggactcac cctcaaacag ttatggggag 3900

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccttagtgaa gttatggtac cagttagaga aagaacccat aataggagca gaaactttct 3960

3901 ggaatcactt caataccatg gtcaatctct ttcttgggta ttatcctcgt ctttgaaaga 3960

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atgtagatgg ggcagccaat agggaaacta aattaggaaa agcaggatat gtaactgaca 4020

3961 tacatctacc ccgtcggtta tccctttgat ttaatccttt tcgtcctata cattgactgt 4020

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gaggaagaca aaaagttgtc cccctaacgg acacaacaaa tcagaagact gagttacaag 4080

4021 ctccttctgt ttttcaacag ggggattgcc tgtgttgttt agtcttctga ctcaatgttc 4080

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 caattcatct agctttgcag gattcgggat tagaagtaaa catagtgaca gactcacaat 4140

4081 gttaagtaga tcgaaacgtc ctaagcccta atcttcattt gtatcactgt ctgagtgtta 4140

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atgcattggg aatcattcaa gcacaaccag ataagagtga atcagagtta gtcagtcaaa 4200

4141 tacgtaaccc ttagtaagtt cgtgttggtc tattctcact tagtctcaat cagtcagttt 4200

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 taatagagca gttaataaaa aaggaaaaag tctacctggc atgggtacca gcacacaaag 4260

4201 attatctcgt caattatttt ttcctttttc agatggaccg tacccatggt cgtgtgtttc 4260

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gaattggagg aaatgaacaa gtagatgggt tggtcagtgc tggaatcagg aaagtactat 4320

4261 cttaacctcc tttacttgtt catctaccca accagtcacg accttagtcc tttcatgata 4320

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttttagatgg aatagataag gcccaagaag aacatgagaa atatcacagt aattggagag 4380

4321 aaaatctacc ttatctattc cgggttcttc ttgtactctt tatagtgtca ttaacctctc 4380

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 caatggctag tgattttaac ctaccacctg tagtagcaaa agaaatagta gccagctgtg 4440

4381 gttaccgatc actaaaattg gatggtggac atcatcgttt tctttatcat cggtcgacac 4440

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ataaatgtca gctaaaaggg gaagccatgc atggacaagt agactgtagc ccaggaatat 4500

4441 tatttacagt cgattttccc cttcggtacg tacctgttca tctgacatcg ggtccttata 4500

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggcagctaga ttgtacacat ttagaaggaa aagttatctt ggtagcagtt catgtagcca 4560

4501 ccgtcgatct aacatgtgta aatcttcctt ttcaatagaa ccatcgtcaa gtacatcggt 4560

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtggatatat agaagcagaa gtaattccag cagagacagg gcaagaaaca gcatacttcc 4620

4561 cacctatata tcttcgtctt cattaaggtc gtctctgtcc cgttctttgt cgtatgaagg 4620

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcttaaaatt agcaggaaga tggccagtaa aaacagtaca tacagacaat ggcagcaatt 4680

4621 agaattttaa tcgtccttct accggtcatt tttgtcatgt atgtctgtta ccgtcgttaa 4680

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tcaccagtac tacagttaag gccgcctgtt ggtgggcggg gatcaagcag gaatttggca 4740

4681 agtggtcatg atgtcaattc cggcggacaa ccacccgccc ctagttcgtc cttaaaccgt 4740

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttccctacaa tccccaaagt caaggagtaa tagaatctat gaataaagaa ttaaagaaaa 4800

4741 aagggatgtt aggggtttca gttcctcatt atcttagata cttatttctt aatttctttt 4800

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa atggcagtat 4860

4801 aatatcctgt ccattctcta gtccgacttg tagaattctg tcgtcatgtt taccgtcata 4860

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag 4920

4861 agtaggtgtt aaaattttct tttcccccct aaccccccat gtcacgtccc ctttcttatc 4920

.......... ....... Orf frame 3 cPPT ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc 4980

4921 atctgtatta tcgttgtctg tatgtttgat ttcttaatgt ttttgtttaa tgtttttaag 4980

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaaattttcg ggtttattac agggacagca gagatccagt ttggaaagga ccagcaaagc 5040

4981 ttttaaaagc ccaaataatg tccctgtcgt ctctaggtca aacctttcct ggtcgtttcg 5040

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata aaagtagtgc 5100

5041 aggagacctt tccacttccc cgtcatcatt atgttctatt atcactgtat tttcatcacg 5100

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 caagaagaaa agcaaagatc atcagggatt atggaaaaca gatggcaggt gatgattgtg 5160

5101 gttcttcttt tcgtttctag tagtccctaa taccttttgt ctaccgtcca ctactaacac 5160

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tggcaagtag acaggatgag gattaacaca tggaaaagat tagtaaaaca ccatatgtat 5220

5161 accgttcatc tgtcctactc ctaattgtgt accttttcta atcattttgt ggtatacata 5220

......... Orf frame 3 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atttcaagga aagctaagga ctggttttat agacatcact atgaaagtac taatccaaaa 5280

5221 taaagttcct ttcgattcct gaccaaaata tctgtagtga tactttcatg attaggtttt 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ataagttcag aagtacacat cccactaggg gatgctaaat tagtaataac aacatattgg 5340

5281 tattcaagtc ttcatgtgta gggtgatccc ctacgattta atcattattg ttgtataacc 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggtctgcata caggagaaag agactggcat ttgggtcagg gagtctccat agaatggagg 5400

5341 ccagacgtat gtcctctttc tctgaccgta aacccagtcc ctcagaggta tcttacctcc 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aaaaagagat atagcacaca agtagaccct gacctagcag accaactaat tcatctgcac 5460

5401 tttttctcta tatcgtgtgt tcatctggga ctggatcgtc tggttgatta agtagacgtg 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tattttgatt gtttttcaga atctgctata agaaatacca tattaggacg tatagttagt 5520

5461 ataaaactaa caaaaagtct tagacgatat tctttatggt ataatcctgc atatcaatca 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cctaggtgtg aatatcaagc aggacataac aaggtaggat ctctacagta cttggcacta 5580

5521 ggatccacac ttatagttcg tcctgtattg ttccatccta gagatgtcat gaaccgtgat 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gcagcattaa taaaaccaaa acagataaag ccacctttgc ctagtgttag gaaactgaca 5640

5581 cgtcgtaatt attttggttt tgtctatttc ggtggaaacg gatcacaatc ctttgactgt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gaggacagat ggaacaagcc ccagaagacc aagggccaca gagggagcca tacaatgaat 5700

5641 ctcctgtcta ccttgttcgg ggtcttctgg ttcccggtgt ctccctcggt atgttactta 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ggacactaga gcttttagag gaacttaaga gtgaagctgt tagacatttt cctaggatat 5760

5701 cctgtgatct cgaaaatctc cttgaattct cacttcgaca atctgtaaaa ggatcctata 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ggctccataa cttaggacaa catatctatg aaacttacgg ggatacttgg gcaggagtgg 5820

5761 ccgaggtatt gaatcctgtt gtatagatac tttgaatgcc cctatgaacc cgtcctcacc 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SalI(5875)

5821 aagccataat aagaattctg caacaactgc tgtttatcca tttcagaatt gggtgtcgac 5880

5821 ttcggtatta ttcttaagac gttgttgacg acaaataggt aaagtcttaa cccacagctg 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 atagcagaat aggcgttact cgacagagga gagcaagaaa tggagccagt agatcctaga 5940

5881 tatcgtctta tccgcaatga gctgtctcct ctcgttcttt acctcggtca tctaggatct 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ctagagccct ggaagcatcc aggaagtcag cctaaaactg cttgtaccaa ttgctattgt 6000

5941 gatctcggga ccttcgtagg tccttcagtc ggattttgac gaacatggtt aacgataaca 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aaaaagtgtt gctttcattg ccaagtttgt ttcatgacaa aagccttagg catctcctat 6060

6001 tttttcacaa cgaaagtaac ggttcaaaca aagtactgtt ttcggaatcc gtagaggata 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ggcaggaaga agcggagaca gcgacgaaga gctcatcaga acagtcagac tcatcaagct 6120

6061 ccgtccttct tcgcctctgt cgctgcttct cgagtagtct tgtcagtctg agtagttcga 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tctctatcaa agcagtaagt agtacatgta atgcaaccta taatagtagc aatagtagca 6180

6121 agagatagtt tcgtcattca tcatgtacat tacgttggat attatcatcg ttatcatcgt 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ttagtagtag caataataat agcaatagtt gtgtggtcca tagtaatcat agaatatagg 6240

6181 aatcatcatc gttattatta tcgttatcaa cacaccaggt atcattagta tcttatatcc 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 aaaatattaa gacaaagaaa aatagacagg ttaattgata gactaataga aagagcagaa 6300

6241 ttttataatt ctgtttcttt ttatctgtcc aattaactat ctgattatct ttctcgtctt 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gacagtggca atgagagtga aggagaagta tcagcacttg tggagatggg ggtggaaatg 6360

6301 ctgtcaccgt tactctcact tcctcttcat agtcgtgaac acctctaccc ccacctttac 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gggcaccatg ctccttggga tattgatgat ctgtagttga gcggccgctg atcttcagac 6420

6361 cccgtggtac gaggaaccct ataactacta gacatcaact cgccggcgac tagaagtctg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ttggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa 6480

6421 aacctcctcc tctatactcc ctgttaacct cttcacttaa tatatttata tttcatcatt 6480

.......... .......... ......... Orf frame 3 ......... ..........

.......... .......... .......... .......... .......... ..........

6481 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa 6540

6481 tttaacttgg taatcctcat cgtgggtggt tccgtttctc ttctcaccac gtctctcttt 6540

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta 6600

6541 tttctcgtca cccttatcct cgaaacaagg aacccaagaa ccctcgtcgt ccttcgtgat 6600

.......... ......... Orf frame 3 RRE ......... ..........

.......... .......... .......... .......... .......... ..........

6601 tgggcgcagc ctcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc 6660

6601 acccgcgtcg gagttactgc gactgccatg tccggtctgt taataacaga ccatatcacg 6660

.......... ....... Orf frame 3 RRE ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag 6720

6661 tcgtcgtctt gttaaacgac tcccgataac tccgcgttgt cgtagacaac gttgagtgtc 6720

.......... ....... Orf frame 3 RRE ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tctggggcat caagcagctc caagcaagaa tcctagctgt ggaaagatac ctaaaggatc 6780

6721 agaccccgta gttcgtcgag gttcgttctt aggatcgaca cctttctatg gatttcctag 6780

.......... ....... Orf frame 3 RRE ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aacagctcct agggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt 6840

6781 ttgtcgagga tccctaaacc ccaacgagac cttttgagta aacgtggtga cgacacggaa 6840

RRE ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ggaatgctag ttggagtaat aaatctctgg aacagatctg gaatcacacg acctggatgg 6900

6841 ccttacgatc aacctcatta tttagagacc ttgtctagac cttagtgtgc tggacctacc 6900

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc 6960

6901 tcaccctgtc tctttaattg ttaatgtgtt cgaattatgt gaggaattaa cttcttagcg 6960

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt 7020

6961 ttttggtcgt tcttttctta cttgttctta ataaccttaa tctatttacc cgttcaaaca 7020

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag 7080

7021 ccttaaccaa attgtattgt ttaaccgaca ccatatattt taataagtat tactatcatc 7080

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc 7140

7081 ctccgaacca tccaaattct tatcaaaaac gacatgaaag atatcactta tctcaatccg 7140

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 agggatattc accattatcg tttcagaccc acctcccaat cccgagggga cccgacaggc 7200

7141 tccctataag tggtaatagc aaagtctggg tggagggtta gggctcccct gggctgtccg 7200

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga 7260

7201 ggcttcctta tcttcttctt ccacctctct ctctgtctct gtctaggtaa gctaatcact 7260

.......... ......... Orf frame 3 ......... .......... ..........

BamHI(7263) ........ .......... .......... .......... ..........

7261 acggatcctt ggcacttatc tgggacgatc tgcggagcct gtgcctcttc agctaccacc 7320

7261 tgcctaggaa ccgtgaatag accctgctag acgcctcgga cacggagaag tcgatggtgg 7320

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gcttgagaga cttactcttg attgtaacga ggattgtgga acttctggga cgcagggggt 7380

7321 cgaactctct gaatgagaac taacattgct cctaacacct tgaagaccct gcgtccccca 7380

........ HIV_Rev_NES Orf frame 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7381 gggaagccct caaatattgg tggaatctcc tacaatattg gagtcaggag ctaaagaata 7440

7381 cccttcggga gtttataacc accttagagg atgttataac ctcagtcctc gatttcttat 7440

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 gtgctgttag cttgctcaat gccacagcca tagcagtagc tgaggggaca gatagggtta 7500

7441 cacgacaatc gaacgagtta cggtgtcggt atcgtcatcg actcccctgt ctatcccaat 7500

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tagaagtagt acaaggagct tgtagagcta ttcgccacat acctagaaga ataagacagg 7560

7501 atcttcatca tgttcctcga acatctcgat aagcggtgta tggatcttct tattctgtcc 7560

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gcttggaaag gattttgcta taagatgggt ggcaagtggt caaaaagtag tgtgattgga 7620

7561 cgaacctttc ctaaaacgat attctaccca ccgttcacca gtttttcatc acactaacct 7620

......... Orf frame 3 ......... ......... Orf frame 1 .........

.......... .......... .......... .......... .......... ..........

7621 tggcctactg taagggaaag aatgagacga gctgagccag cagcagatgg ggtgggagca 7680

7621 accggatgac attccctttc ttactctgct cgactcggtc gtcgtctacc ccaccctcgt 7680

.......... ......... Orf frame 1 ......... .......... ..........

XhoI(7685) .......... .......... .......... .......... ..........

7681 gtatctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat 7740

7681 catagagctc tggatctttt tgtacctcgt tagtgttcat cgttatgtcg tcgatggtta 7740

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct 7800

7741 cgactaacac ggaccgatct tcgtgttctc ctcctcctcc acccaaaagg tcagtgtgga 7800

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 7860

7801 gtccatggaa attctggtta ctgaatgttc cgtcgacatc tagaatcggt gaaaaatttt 7860

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg 7920

7861 cttttccccc ctgaccttcc cgattaagtg agggttgctt ctgttctata ggaactagac 7920

cPPT U3PPT ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg 7980

7921 acctagatgg tgtgtgttcc gatgaaggga ctaaccgtct tgatgtgtgg tcccggtccc 7980

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag 8040

7981 tagtctatag gtgactggaa acctaccacg atgttcgatc atggtcaact cgttctcttc 8040

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg 8100

8041 catcttcttc ggttacttcc tctcttgtgg gcgaacaatg tgggacactc ggacgtaccc 8100

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat 8160

8101 tacctactgg gcctctctct tcataatctc acctccaaac tgtcggcgga tcgtaaagta 8160

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 cacatggccc gagagctgca tccggagtac ttcaagaact gctgagcggc cgccccggtg 8220

8161 gtgtaccggg ctctcgacgt aggcctcatg aagttcttga cgactcgccg gcggggccac 8220

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 accttcagac cttggcactg gaggtggccc ggcagaagcg cggcatcgtg gatcagtgct 8280

8221 tggaagtctg gaaccgtgac ctccaccggg ccgtcttcgc gccgtagcac ctagtcacga 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 gcaccagcat ctgctctctc taccaactgg agaactactg caactaggcc caccactacc 8340

8281 cgtggtcgta gacgagagag atggttgacc tcttgatgac gttgatccgg gtggtgatgg 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 ctgtccaccc ctctgcaatg aataaaacct ttgaaagagc actacaagtt gtgtgtacat 8400

8341 gacaggtggg gagacgttac ttattttgga aactttctcg tgatgttcaa cacacatgta 8400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 gcgtgcatgt gcatatgtgg tgcgggggga acatgagtgg ggctggctgg agtggcgatg 8460

8401 cgcacgtaca cgtatacacc acgcccccct tgtactcacc ccgaccgacc tcaccgctac 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt 8520

8461 tattcgacag tttgtactct taagaacttc tgctttcccg gagcactatg cggataaaaa 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XbaI(8555) .......... ..........

8521 ataggttaat gtcatgataa taatggtttc ttagtctaga attaattccg tgtattctat 8580

8521 tatccaatta cagtactatt attaccaaag aatcagatct taattaaggc acataagata 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt 8640

8581 tcacagtgga tttagcatac acatactatg tattccaata cataattaac atcggcgcaa 8640

Sp6_primer .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat 8700

8641 gattgctgtt atacatgttc ggattaacac atcgtagacc gaatgacttc gtctgggata 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg 8760

8701 gtagagagag catttgacgg cagtctcagc caaaccaacc tgcttggaag actcaaagac 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NheI(8815)

8761 gtaacgccgt tccgcacccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc 8820

8761 cattgcggca aggcgtgggg cctttaccag tcgcttggtt agtcgtccca gtagcgatcg 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct 8880

8821 gtctaggaga tgcggcctgc gtagcaccgg ccgtagtggc cgcggtgtcc acgccaacga 8880

.......... .......... ...... tet(564-300) pBRrevBam_primer ......

.......... .......... .......... .......... .......... ..........

8881 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg 8940

8881 ccgcggatat agcggctgta gtggctaccc cttctagccc gagcggtgaa gcccgagtac 8940

...... pBRrevBam_primer tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc 9000

8941 tcgcgaacaa agccgcaccc ataccaccgt ccggggcacc ggccccctga caacccgcgg 9000

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg 9060

9001 tagaggaacg tacgtggtaa ggaacgccgc cgccacgagt tgccggagtt ggatgatgac 9060

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gatatggtgc actctcagta 9120

9061 ccgacgaagg attacgtcct cagcgtattc cctctcgcag ctataccacg tgagagtcat 9120

......... tet(564-300) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 9180

9121 gttagacgag actacggcgt atcaattcgg tcggggctgt gggcggttgt gggcgactgc 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 9240

9181 gcgggactgc ccgaacagac gagggccgta ggcgaatgtc tgttcgacac tggcagaggc 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 9300

9241 cctcgacgta cacagtctcc aaaagtggca gtagtggctt tgcgcgctct gctttcccgg 9300

pGEX_3_primer ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 9360

9301 agcactatgc ggataaaaat atccaattac agtactatta ttaccaaaga atctgcagtc 9360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 9420

9361 caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa 9420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 9480

9421 gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt 9480

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 9540

9481 ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa 9540

.......... .......... .... Orf frame 3 Ampicillin .... ..........

.......... .......... .......... .......... .......... ..........

9541 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 9600

9541 cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca 9600

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 9660

9601 acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa 9660

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 9720

9661 aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc 9720

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 9780

9721 ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct 9780

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 9840

9781 tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt 9840

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 9900

9841 ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact 9900

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 9960

9901 gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt 9960

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 10020

9961 gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt 10020

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... ......... FspI(10053) ......... ..........
gaactactta
10021 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 10080

10021 ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat 10080

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........
gcaggaccac
10081 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 10140

10081 gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg 10140

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........
gccggtgagc
10141 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 10200

10141 aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg 10200

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........
cgtatcgtag
10201 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 10260

10201 cacccagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc 10260

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........
atcgctgaga
10261 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 10320

10261 aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct 10320

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 10380

10321 atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa 10380

.... Orf frame 3 Ampicillin .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 10440

10381 tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 10500

10441 tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc 10500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 10560

10501 ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt 10560

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

10561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 10620

10561 gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa 10620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 10680

10621 aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa gatcacatcg 10680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 10740

10681 gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt 10740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 10800

10741 aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt 10800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 10860

10801 ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg 10860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 10920

10861 ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt 10920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 10980

10921 cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt 10980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

10981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 11040

10981 gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc 11040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

11041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 11100

11041 ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg 11100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

11101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 11160

11101 ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac 11160

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 11220

11161 gagtgtacaa gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac 11220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11221 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 11280

11221 tcactcgact atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc 11280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11281 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 11340

11281 ttcgccttct cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta 11340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11341 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 11400

11341 cgtcgacacc ttacacacag tcaatcccac acctttcagg ggtccgaggg gtcgtccgtc 11400

........ pBABE_3_primer .... SV40_enhancer SV40_promoter ......

.......... .......... .......... .......... .......... ..........

11401 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 11460

11401 ttcatacgtt tcgtacgtag agttaatcag tcgttggtcc acacctttca ggggtccgag 11460

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

11461 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 11520

11461 gggtcgtccg tcttcatacg tttcgtacgt agagttaatc agtcgttggt atcagggcgg 11520

.......... SV40_enhancer SV40_promoter SV40_promoter ........

.......... .......... .......... .......... .......... ..........

11521 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 11580

11521 ggattgaggc gggtagggcg gggattgagg cgggtcaagg cgggtaagag gcggggtacc 11580

... SV40_enhancer SV40_promoter SV40_promoter SV40_origin ...

.......... .......... .......... .......... .......... ..........

11581 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 11640

11581 gactgattaa aaaaaataaa tacgtctccg gctccggcgg agccggagac tcgataaggt 11640

SV40_origin SV40_promoter SV40_promoter SV40pro_F_primer ....

.......... ......... StuI(11667) ......... .......... ..........

11641 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac 11700

11641 cttcatcact cctccgaaaa aacctccgga tccgaaaacg tttttcgaac ctgtgttctg 11700

.......... ........ SV40_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

11701 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt 11760

11701 tccgaacgct ctatacaaac tcttatggtg aaatagggcg cagtccctct ccgtcacgca 11760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11761 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg 11820

11761 tttttctgcg cctgagtaca ctttatgacc aaaaatcacg cggtctagag atattagagc 11820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11821 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc 11880

11821 gcgttggata aaaggggagc ttgtgaaaaa ttcggcatct atttgtccga ccctgtgaag 11880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11881 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg 11940

11881 tgtactcgct ttttatgtag cagtggaccc tgtacaacgt ctaggtacgt gcatttgagc 11940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11941 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc 12000

11941 gttcggctga ctacggaaga cttgttacct ttccgtaata acggcattcg gcaccgccag 12000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12001 tggtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta 12060

12001 accatggccc acgcaatgac cgcgcacttg acccataagc agtacagcta tggcaaacat 12060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12061 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg 12120

12061 aaaggtcgat gctagtgctg ttggtcgcgc tcgaatttca cgactttgcg cgtcttccgc 12120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12121 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga 12180

12121 taccgcttcc gaagtagcaa taactactgg accacctatg gccaccatga cgccaacgct 12180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12181 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc 12240

12181 aagcacttta cataggtttt cgcgtgaaac agtggtagaa gcgttttggc cgaccagcag 12240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12241 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg 12300

12241 gcgaccaact actgatacaa caactatagg gcgttctatg gacctaactt gtcggcaccc 12300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12301 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac 12360

12301 tatacccgca gcataagcag ggcggttaga ggccagcgat tagaaaagtt gcggaccgtg 12360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12361 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg 12420

12361 acggcccgca acaagaaaaa ttgaagtccg cccaatgtta tcaaaggtca ttcataagac 12420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12421 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa 12480

12421 ctccgacgta ggtactgtgt ccgtttggac tcgctttggg acaagtttgg ggcgaaattt 12480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12481 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag 12540

12481 gtaggacttt ggagctgcga tcaggcggcg aaattagtgc cgcgtgttgg cggacacgtc 12540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12541 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg 12600

12541 agccgggaac taccattttg gtagggagtg accatagcgt actaattggc agactacacc 12600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12601 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc 12660

12601 tagaccgcgc cgtaactggg tgcgctttag gagctgcagg tccgtgcata acactactcg 12660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12661 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac 12720

12661 ctacggcttg catggctgct actaaatatg ctatgccact aaccgatggc accgccgttg 12720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12721 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata 12780

12721 acctaaatac tcacccgggg cctagaaaca cttccttgga atgaagacac cacactgtat 12780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12781 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt 12840

12781 taacctgttt gatggatgtc tctaaatttc gagattccat ttatatttta aaaattcaca 12840

.......... .......... .......... .......... SV40_int ..........

.......... .......... .......... .......... .......... ..........

12841 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac 12900

12841 tattacacaa tttgatgact aagattaaca aacacataaa atctaaggtt ggataccttg 12900

.......... ........ SV40_3_splice ........ .......... ..........

.......... .......... .......... .......... .......... ..........

12901 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga 12960

12901 actacttacc ctcgtcacca ccttacggaa attactcctt ttggacaaaa cgagtcttct 12960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12961 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa 13020

12961 ttacggtaga tcactactac tccgatgacg actgagagtt gtaagatgag gaggtttttt 13020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13021 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca 13080

13021 cttctctttc catcttctgg ggttcctgaa aggaagtctt aacgattcaa aaaactcagt 13080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13081 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc 13140

13081 acgacacaaa tcattatctt gagaacgaac gaaacgataa atgtggtgtt tcctttttcg 13140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13141 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa 13200

13141 acgtgacgat atgttctttt aatacctttt tataagacat tggaaatatt catccgtatt 13200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13201 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat 13260

13201 gtcaatatta gtattgtatg acaaaaaaga atgaggtgtg tccgtatctc acagacgata 13260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13261 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa 13320

13261 attattgata cgagttttta acacatggaa atcgaaaaat taaacatttc cccaattatt 13320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13321 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag 13380

13321 ccttataaac tacatatcac ggaactgatc tctagtatta gtcggtatgg tgtaaacatc 13380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13381 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 13440

13381 tccaaaatga acgaaatttt ttggagggtg tggaggggga cttggacttt gtattttact 13440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

13441 atgcaattgt tgttgtt... .......... .......... .......... .......... 13457

13441 tacgttaaca acaacaa... .......... .......... .......... .......... 13457

.......... .......... .......... .......... .......... ..........