Sequence of pCRT7

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Sequence of pCRT7_CT-TOPO

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Sequence of pCRT7/CT-TOPO : 2702 BP; 658 A; 678 C; 715 G; 651 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ggatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc 60

1 cctagagcta gggcgcttta attatgctga gtgatatccc tctggtgttg ccaaagggag 60

.......... .......... T7_pro .......... .......... ..........

.......... .......... .......... AflII(98) .......... ..........

61 tagaaataat tttgtttaac tttaagaagg aattgccctt aagggcaatt cgaagcttga 120

61 atctttatta aaacaaattg aaattcttcc ttaacgggaa ttcccgttaa gcttcgaact 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AgeI(167) ..........

121 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca 180

121 tccattcgga tagggattgg gagaggagcc agagctaaga tgcgcatggc cagtagtagt 180

.......... .......... V5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ccatcaccat tgagtttaaa ctatatagaa taaaagaaga aaccttagct gagcaataac 240

181 ggtagtggta actcaaattt gatatatctt attttcttct ttggaatcga ctcgttattg 240

6xHis .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa 300

241 atcgtattgg ggaaccccgg agatttgccc agaactcccc aaaaaacgac tttcctcctt 300

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ctatatccgg attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 360

301 gatataggcc taattgcgaa tgttaaatcc accgtgaaaa gcccctttac acgcgccttg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 420

361 gggataaaca aataaaaaga tttatgtaag tttatacata ggcgagtact ctgttattgg 420

.......... .......... .......... zeo AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

421 ctgataaatg cttcaataat gtgaggaggg ccaccatggc caagttgacc agtgccgttc 480

421 gactatttac gaagttatta cactcctccc ggtggtaccg gttcaactgg tcacggcaag 480

.......... .......... zeo ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........
< td>ctggaccgac
481 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt cggctcgggt 540

481 gccacgagtg gcgcgcgctg cagcggcctc gccagctcaa gacctggctg gccgagccca 540

.......... ....... zeo bleo sh_ble ....... .......... ..........

SmaI(546) .......... .......... .......... .......... ..........
< td>ccgggacgac
541 tctcccggga cttcgtggag gacgacttcg ccggtgtggt gtgaccctgt 600

541 agagggccct gaagcacctc ctgctgaagc ggccacacca ggccctgctg cactgggaca 600

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>cctggcctgg
601 tcatcagcgc ggtccaggac caggtggtgc cggacaacac gtgtgggtgc 660

601 agtagtcgcg ccaggtcctg gtccaccacg gcctgttgtg ggaccggacc cacacccacg 660

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>gtccacgaac
661 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt ttccgggacg 720

661 cgccggacct gctcgacatg cggctcacca gcctccagca caggtgcttg aaggccctgc 720

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>ggggcgggag
721 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ttcgccctgc 780

721 ggaggcccgg ccggtactgg ctctagccgc tcgtcggcac ccccgccctc aagcgggacg 780

.......... ....... zeo bleo sh_ble ....... .......... ..........

EagI(787) .......... .......... .......... .......... ..........
< td>gcaggactga
781 gcgacccggc cggcaactgc gtgcacttcg tggccgagga cacattgaaa 840

781 cgctgggccg gccgttgacg cacgtgaagc accggctcct cgtcctgact gtgtaacttt 840

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 900

841 ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa aacgccgtaa 900

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

901 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 960

901 aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac gacttctagt 960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 1020

961 caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct aggaactctc 1020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 1080

1021 aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg atacaccgcg 1080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 1140

1081 ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg tgataagagt 1140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 1200

1141 cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac cgtactgtca 1200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 1260

1201 ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt tgaatgaaga 1260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 1320

1261 ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc ccctagtaca 1320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagagtga 1380

1321 ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc tgctctcact 1380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... FspI(1415) .......... ..........

1381 caccacgatg cctgtagcaa tgccaacaac gttgcgcaaa ctattaactg gcgaactact 1440

1381 gtggtgctac ggacatcgtt acggttgttg caacgcgttt gataattgac cgcttgatga 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 1500

1441 atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc aacgtcctgg 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 1560

1501 tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac ctcggccact 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 1620

1561 cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga gggcatagca 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 1680

1621 tcaatagatg tgctgcccct cagtccgttg atacctactt gctttatctg tctagcgact 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 1740

1681 ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga gtatatatga 1740

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 1800

1741 aatctaacta aattttgaag taaaaattaa attttcctag atccacttct aggaaaaact 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 1860

1801 attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca gtctggggca 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 1920

1861 tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga cgacgaacgt 1920

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

1921 aacaaaaaaa cgcgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1980

1921 ttgttttttt gcgcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 2040

1981 aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa gatcacatcg 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 2100

2041 gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 2160

2101 aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 2220

2161 ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 2280

2221 ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 2340

2281 cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caggagacgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 2400

2341 gtcctctgcg tgctccctcg aaggtccccc tttgcggacc atagaaatat caggacagcc 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 2460

2401 caaagcggtg gagactgaac tcgcagctaa aaacactacg agcagtcccc ccgcctcgga 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 2520

2461 tacctttttg cggtcgttgc gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg 2520

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 2580

2521 agtgtacaag aaaggacgca ataggggact aagacaccta ttggcataat ggcggaaact 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 2640

2581 cactcgacta tggcgagcgg cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 2700

2641 tcgccttctc gcgggttatg cgtttggcgg agaggggcgc gcaaccggct aagtaattac 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ca........ .......... .......... .......... .......... .......... 2702

2701 gt........ .......... .......... .......... .......... .......... 2702

.......... .......... .......... .......... .......... ..........