Sequence of pCRT7

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Sequence of pCRT7_NT-TOPO

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Sequence of pCRT7/NT-TOPO : 2870 BP; 716 A; 703 C; 715 G; 736 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct 60

1 ctagagctag ggcgctttaa ttatgctgag tgatatccct ctggtgttgc caaagggaga 60

.......... .......... T7_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat 120

61 tctttattaa aacaaattga aattcttcct ctatatgtat acgccccaag agtagtagta 120

.......... ....... T7_transl_en_RBS ....... .......... 6xHis

.......... .......... .......... .......... .......... ..........

121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac 180

121 gtagtagtac cataccgatc gtactgacca cctgtcgttt acccagccct agacatgctg 180

6xHis Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK

BamHI AflII02) EcoRI(211),BstBI(215),HindIII(218) ......

181 gatgacgata aggatccaac ccttaagggc gaattcgaag cttgatccgg ctgctaacaa 240

181 ctactgctat tcctaggttg ggaattcccg cttaagcttc gaactaggcc gacgattgtt 240

Xpress_EK .......... .......... .......... .......... T7_term

.......... .......... .......... .......... .......... ..........

241 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct 300

241 tcgggctttc cttcgactca accgacgacg gtggcgactc gttattgatc gtattgggga 300

.......... .......... T7_term ....... T7_Terminal_pri .......

.......... .......... .......... .......... .......... ..........

301 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc 360

301 accccggaga tttgcccaga actccccaaa aaacgacttt cctccttgat ataggcctag 360

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 420

361 accgcattat cgcttctccg ggcgtggcta gcgggaaggg ttgtcaacgc gtcggactta 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 480

421 ccgcttaccc tgcgcgggac atcgccgcgt aattcgcgcc gcccacacca ccaatgcgcg 480

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

481 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 540

481 tcgcactggc gatgtgaacg gtcgcgggat cgcgggcgag gaaagcgaaa gaagggaagg 540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 600

541 aaagagcggt gcaagcggcc gaaaggggca gttcgagatt tagcccccga gggaaatccc 600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 660

601 aaggctaaat cacgaaatgc cgtggagctg gggttttttg aactaatccc actaccaagt 660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 720

661 gcatcacccg gtagcgggac tatctgccaa aaagcgggaa actgcaacct caggtgcaag 720

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 780

721 aaattatcac ctgagaacaa ggtttgacct tgttgtgagt tgggatagag ccagataaga 780

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 840

781 aaactaaata ttccctaaaa cggctaaagc cggataacca attttttact cgactaaatt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc 900

841 gtttttaaat tgcgcttaaa attgttttat aattgcgaat gttaaatcca ccgtgaaaag 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 960

901 cccctttaca cgcgccttgg ggataaacaa ataaaaagat ttatgtaagt ttatacatag 960

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

961 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 1020

961 gcgagtactc tgttattggg actatttacg aagttattat aactttttcc ttctcatact 1020

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 1080

1021 cataagttgt aaaggcacag cgggaataag ggaaaaaacg ccgtaaaacg gaaggacaaa 1080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 1140

1081 aacgagtggg tctttgcgac cactttcatt ttctacgact tctagtcaac ccacgtgctc 1140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 1200

1141 acccaatgta gcttgaccta gagttgtcgc cattctagga actctcaaaa gcggggcttc 1200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 1260

1201 ttgcaaaagg ttactactcg tgaaaatttc aagacgatac accgcgccat aatagggcat 1260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 1320

1261 aactgcggcc cgttctcgtt gagccagcgg cgtatgtgat aagagtctta ctgaaccaac 1320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 1380

1321 tcatgagtgg tcagtgtctt ttcgtagaat gcctaccgta ctgtcattct cttaatacgt 1380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 1440

1381 cacgacggta ttggtactca ctattgtgac gccggttgaa tgaagactgt tgctagcctc 1440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 1500

1441 ctggcttcct cgattggcga aaaaacgtgt tgtaccccct agtacattga gcggaactag 1500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 1560

1501 caacccttgg cctcgactta cttcggtatg gtttgctgct cgcactgtgg tgctacggac 1560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 1620

1561 atcgttaccg ttgttgcaac gcgtttgata attgaccgct tgatgaatga gatcgaaggg 1620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 1680

1621 ccgttgttaa ttatctgacc tacctccgcc tatttcaacg tcctggtgaa gacgcgagcc 1680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 1740

1681 gggaaggccg accgaccaaa taacgactat ttagacctcg gccactcgca cccagagcgc 1740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 1800

1741 catagtaacg tcgtgacccc ggtctaccat tcgggagggc atagcatcaa tagatgtgct 1800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 1860

1801 gcccctcagt ccgttgatac ctacttgctt tatctgtcta gcgactctat ccacggagtg 1860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 1920

1861 actaattcgt aaccattgac agtctggttc aaatgagtat atatgaaatc taactaaatt 1920

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 1980

1921 ttgaagtaaa aattaaattt tcctagatcc acttctagga aaaactatta gagtactggt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 2040

1981 tttagggaat tgcactcaaa agcaaggtga ctcgcagtct ggggcatctt ttctagtttc 2040

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

2041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 2100

2041 ctagaagaac tctaggaaaa aaagacgcgc attagacgac gaacgtttgt ttttttggtg 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 2160

2101 gcgatggtcg ccaccaaaca aacggcctag ttctcgatgg ttgagaaaaa ggcttccatt 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 2220

2161 gaccgaagtc gtctcgcgtc tatggtttat gacaagaaga tcacatcggc atcaatccgg 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 2280

2221 tggtgaagtt cttgagacat cgtggcggat gtatggagcg agacgattag gacaatggtc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 2340

2281 accgacgacg gtcaccgcta ttcagcacag aatggcccaa cctgagttct gctatcaatg 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 2400

2341 gcctattccg cgtcgccagc ccgacttgcc ccccaagcac gtgtgtcggg tcgaacctcg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 2460

2401 cttgctggat gtggcttgac tctatggatg tcgcactcga tactctttcg cggtgcgaag 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 2520

2461 ggcttccctc tttccgcctg tccataggcc attcgccgtc ccagccttgt cctctcgcgt 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 2580

2521 gctccctcga aggtccccct ttgcggacca tagaaatatc aggacagccc aaagcggtgg 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 2640

2581 agactgaact cgcagctaaa aacactacga gcagtccccc cgcctcggat acctttttgc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 2700

2641 ggtcgttgcg ccggaaaaat gccaaggacc ggaaaacgac cggaaaacga gtgtacaaga 2700

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 2760

2701 aaggacgcaa taggggacta agacacctat tggcataatg gcggaaactc actcgactat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 2820

2761 ggcgagcggc gtcggcttgc tggctcgcgt cgctcagtca ctcgctcctt cgccttctcg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc .......... 2870

2821 cgggttatgc gtttggcgga gaggggcgcg caaccggcta agtaattacg .......... 2870

.......... .......... .......... .......... .......... ..........