Sequence of pDEST10

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Sequence of pDEST10

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Sequence of pDEST10 : 6708 BP; 1748 A; 1579 C; 1587 G; 1794 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc 60

1 ggggcctact tcaccaagcg taggagccaa aagaccttcc gctcgtagca aacaagcggg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aggactctag ctatagttct agtggttggc tacgtatact ccggaatatt aatagatcat 120

61 tcctgagatc gatatcaaga tcaccaaccg atgcatatga ggccttataa ttatctagta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac 180

121 cctctattaa ttttactatt ggtagagcgt ttatttattc ataaaatgac aaaagcattg 180

...... polyhedrin_fwd_pri PH_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg 240

181 tcaaaacatt atttttttgg atatttataa ggcctaataa gtatggcagg gtggtagccc 240

.......... PH_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cgcggatctc ggtccgaaac catgtcgtac taccatcacc atcaccatca cgattacgat 300

241 gcgcctagag ccaggctttg gtacagcatg atggtagtgg tagtggtagt gctaatgcta 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 atcccaacga ccgaaaacct gtattttcag ggcatcacaa gtttgtacaa aaaagctgaa 360

301 tagggttgct ggcttttgga cataaaagtc ccgtagtgtt caaacatgtt ttttcgactt 360

.......... .......... .......... .......... attR1 ..........

.......... .......... .......... .......... .......... ..........

361 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag 420

361 gctctttgca ttttactata tttatagtta tataatttaa tctaaaacgt attttttgtc 420

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgct aagttggcag 480

421 tgatgtatta tgacattttg tgttgtatag gtcagtgata ccgccggcga ttcaaccgtc 480

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac ttcgcagaat 540

481 gtagtgggct gcgtgaaacg cggcttattt atggacactg ccttctagtg aagcgtctta 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat 600

541 tttatttagg accacaggga caactatggc ccttcgggac ccggttgaaa accgctttta 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac 660

601 ctctgcaact agccgtgcat tctccaaggt tgaaagtggt attactttat tctagtgatg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa 720

661 gcccgcataa aaaactcaat agctctaaaa gtcctcgatt ccttcgattt tacctctttt 720

.......... .......... .......... .......... ........ ORF 3 CAT/CamR

.......... .......... .......... .......... .......... ..........

721 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 780

721 tttagtgacc tatatggtgg caactatata gggttaccgt agcatttctt gtaaaactcc 780

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... PvuII(824) ..........

781 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 840

781 gtaaagtcag tcaacgagtt acatggatat tggtctggca agtcgaccta taatgccgga 840

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 900

841 aaaatttctg gcatttcttt ttattcgtgt tcaaaatagg ccggaaataa gtgtaagaac 900

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga 960

901 gggcggacta cttacgagta ggccttaagg cataccgtta ctttctgcca ctcgaccact 960

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 1020

961 ataccctatc acaagtggga acaatgtggc aaaaggtact cgtttgactt tgcaaaagta 1020

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 1080

1021 gcgagacctc acttatggtg ctgctaaagg ccgtcaaaga tgtgtatata agcgttctac 1080

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 1140

1081 accgcacaat gccacttttg gaccggataa agggatttcc caaataactc ttatacaaaa 1140

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 1200

1141 agcagagtcg gttagggacc cactcaaagt ggtcaaaact aaatttgcac cggttatacc 1200

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 1260

1201 tgttgaagaa gcgggggcaa aagtggtacc cgtttataat atgcgttccg ctgttccacg 1260

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa 1320

1261 actacggcga ccgctaagtc caagtagtac ggcagacact accgaaggta cagccgtctt 1320

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat 1380

1321 acgaattact taatgttgtc atgacgctac tcaccgtccc gccccgcatt tgcgcaccta 1380

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa 1440

1381 ggccgaatga ttttcggtct attgtcatac gcataaacgc gcgactaaaa acgccatatt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg 1500

1441 cttatatatg actatacata tgggcttcat acagtttttc tccacacgat acttcgtcgc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat 1560

1501 ataatgtcac tgtcaactgt cgctgtcgat agtcaacgag ttccgtatat actacagtta 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc 1620

1561 tagaggccag accattcgtg ttggtacgtc ttacttcggg cagcagacgc acggcttgcg 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc 1680

1621 acctttcgcc ttttagtcct tccctaccga ctccagcggg ccaaataact ttacttgccg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag 1740

1681 agaaaacgac tgctcttgtc cctgaccact ttacgtcaaa ttccaaatgt ggatattttc 1740

.......... .......... .......... .......... ccdB ..........

.......... .......... .......... .......... .......... SmaI(1795)

1741 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg 1800

1741 tctctcggca atagcagaca aacacctaca tgtctcacta taataactgt gcgggcccgc 1800

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact 1860

1801 tgcctaccac tagggggacc ggtcacgtgc agacgacagt ctatttcaga gggcacttga 1860

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag 1920

1861 aatgggccac cacgtatagc ccctactttc gaccgcgtac tactggtggc tataccggtc 1920

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat 1980

1921 acacggccag aggcaatagc cccttcttca ccgactagag tcggtggcgc ttttactgta 1980

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag 2040

1981 gtttttgcgg taattggact acaagacccc ttatatttac agtccgaggg aatatgtgtc 2040

.......... ccdB .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct 2100

2041 ggtcagacgt ccagctggta tcactgacct atacaacaca aaatgtcata atacatcaga 2100

.......... .......... ......... attR2 attR1 ......... ..........

.......... .......... .......... .......... .......... ..........

2101 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt 2160

2101 caaaaaatac gttttagatt aaattatata actataaata tagtaaaatg caaagagcaa 2160

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cagctttctt gtacaaagtg gtgatgccat ggatccggaa ttcaaaggcc tacgtcgacg 2220

2161 gtcgaaagaa catgtttcac cactacggta cctaggcctt aagtttccgg atgcagctgc 2220

.......... .......... .......... .......... .......... ..........

SacI,SpeI(2227) BstBI(2243),XbaI(2248) XhoI(2263) ......

2221 agctcaacta gtgcggccgc tttcgaatct agagcctgca gtctcgaggc atgcggtacc 2280

2221 tcgagttgat cacgccggcg aaagcttaga tctcggacgt cagagctccg tacgccatgg 2280

.......... .......... .......... .......... .......... ..........

HindIII(2281) ...... .......... .......... .......... ..........

2281 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta 2340

2281 ttcgaacagc tcttcatgat ctcctagtat tagtcggtat ggtgtaaaca tctccaaaat 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt 2400

2341 gaacgaaatt ttttggaggg tgtggagggg gacttggact ttgtatttta cttacgttaa 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 2460

2401 caacaacaat tgaacaaata acgtcgaata ttaccaatgt ttatttcgtt atcgtagtgt 2460

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2461 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 2520

2461 ttaaagtgtt tatttcgtaa aaaaagtgac gtaagatcaa caccaaacag gtttgagtag 2520

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... BclI(2545) .......... .......... ..........

2521 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctaggagatc cgaaccagat 2580

2521 ttacatagaa tagtacagac ctagactagt gacgaactcg gatcctctag gcttggtcta 2580

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc 2640

2581 ttcactttag atcaaggttt gataaaacag taaaaattaa aagcataatc gaatgctgcg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt 2700

2641 atgtgggtca agggtagata aaacagtgag aagggattta ttaggaattt ttgaggtaaa 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt 2760

2701 ggtggggagg gtcaagggtt gataaaacag gcgggtgtcg ccccgtaaaa agaaggacaa 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt 2820

2761 tacaaaaatt agtttgtagg acggttgagg tacactgttt ggcagtagaa gccgatgaaa 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga 2880

2821 aagagacagt gtcttacttt taaaaagaca gtagagaagc aataattaca aacattaact 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 2940

2881 gacttatagt tgcgaataaa cgtcggactt accgcttacc ctgcgcggga catcgccgcg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 3000

2941 taattcgcgc cgcccacacc accaatgcgc gtcgcactgg cgatgtgaac ggtcgcggga 3000

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 3060

3001 tcgcgggcga ggaaagcgaa agaagggaag gaaagagcgg tgcaagcggc cgaaaggggc 3060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 3120

3061 agttcgagat ttagcccccg agggaaatcc caaggctaaa tcacgaaatg ccgtggagct 3120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 3180

3121 ggggtttttt gaactaatcc cactaccaag tgcatcaccc ggtagcggga ctatctgcca 3180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 3240

3181 aaaagcggga aactgcaacc tcaggtgcaa gaaattatca cctgagaaca aggtttgacc 3240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 3300

3241 ttgttgtgag ttgggataga gccagataag aaaactaaat attccctaaa acggctaaag 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 3360

3301 ccggataacc aattttttac tcgactaaat tgtttttaaa ttgcgcttaa aattgtttta 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 3420

3361 taattgcaaa tgttaaagtc caccgtgaaa agccccttta cacgcgcctt ggggataaac 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 3480

3421 aaataaaaag atttatgtaa gtttatacat aggcgagtac tctgttattg ggactattta 3480

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 3540

3481 cgaagttatt ataacttttt ccttctcata ctcataagtt gtaaaggcac agcgggaata 3540

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

3541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 3600

3541 agggaaaaaa cgccgtaaaa cggaaggaca aaaacgagtg ggtctttgcg accactttca 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 3660

3601 ttttctacga cttctagtca acccacgtgc tcacccaatg tagcttgacc tagagttgtc 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 3720

3661 gccattctag gaactctcaa aagcggggct tcttgcaaaa ggttactact cgtgaaaatt 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 3780

3721 tcaagacgat acaccgcgcc ataatagggc ataactgcgg cccgttctcg ttgagccagc 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 3840

3781 ggcgtatgtg ataagagtct tactgaacca actcatgagt ggtcagtgtc ttttcgtaga 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 3900

3841 atgcctaccg tactgtcatt ctcttaatac gtcacgacgg tattggtact cactattgtg 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 3960

3901 acgccggttg aatgaagact gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 4020

3961 gttgtacccc ctagtacatt gagcggaact agcaaccctt ggcctcgact tacttcggta 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(4073)

4021 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 4080

4021 tggtttgctg ctcgcactgt ggtgctacgg acatcgttac cgttgttgca acgcgtttga 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 4140

4081 taattgaccg cttgatgaat gagatcgaag ggccgttgtt aattatctga cctacctccg 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 4200

4141 cctatttcaa cgtcctggtg aagacgcgag ccgggaaggc cgaccgacca aataacgact 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 4260

4201 atttagacct cggccactcg cacccagagc gccatagtaa cgtcgtgacc ccggtctacc 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 4320

4261 attcgggagg gcatagcatc aatagatgtg ctgcccctca gtccgttgat acctacttgc 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 4380

4321 tttatctgtc tagcgactct atccacggag tgactaattc gtaaccattg acagtctggt 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 4440

4381 tcaaatgagt atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 4500

4441 ccacttctag gaaaaactat tagagtactg gttttaggga attgcactca aaagcaaggt 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 4560

4501 gactcgcagt ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc 4560

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4561 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 4620

4561 gcattagacg acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 4680

4621 agttctcgat ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 4740

4681 atgacaggaa gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 4800

4741 atgtatggag cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 4860

4801 agaatggccc aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 4920

4861 ccccccaagc acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 4980

4921 tgtcgcactc gtaactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 5040

4981 ccattcgccg tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 5100

5041 catagaaata tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 5160

5101 gagcagtccc cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 5220

5161 ccggaaaacg accggaaaac gagtgtacaa gaaaggacgc aataggggac taagacacct 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 5280

5221 attggcataa tggcggaaac tcactcgact atggcgagcg gcgtcggctt gctggctcgc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 5340

5281 gtcgctcagt cactcgctcc ttcgccttct cgcggactac gccataaaag aggaatgcgt 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg 5400

5341 agacacgcca taaagtgtgg cgtctggtcg gcgcattgga ccgttttagc caatgccaac 5400

.......... .......... .......... .......... Tn7_att ..........

.......... .......... .......... .......... .......... ..........

5401 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa 5460

5401 tcattattta cctacgggac gcattcgccc acacccgcct gttatttcag aatttgactt 5460

.......... Tn7_att .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga 5520

5461 gttttatcta gatttgatac tgttatttca gaatttgatc tgtcttatca acatttgact 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact 5580

5521 ttagtcaggt caatacgaca ctttttcgta tgacctgaaa acaataccga tttcgtttga 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg 5640

5581 gaagtaaaag acttcacgtt taacgggcag cataatttct ccccgcaccg gttcccgtac 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... SacII(5688) .........

5641 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 5700

5641 catttctgat ataagcgccg caacactgtt aaatggcttg ttgaggcgcc ggcccttcgg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac 5760

5701 ctagagccga acttgcttaa caatccaccg ccatgaaccc agctatagtt tcacgtagtg 5760

.......... .......... .......... .......... gent ..........

.......... .......... .......... .......... .......... ..........

5761 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc 5820

5761 aagaagggca tacgggttga aacatatctc tcggtgacgc cctagcagtg gcattagacg 5820

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag 5880

5821 aacgtgcatc tagtgtattc gtggttcgcg caaccggagt acgaactcct ctaactactc 5880

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat 5940

5881 gcgccaccgt tacgggacgg aggccacgag cggcctctga cgctctagta tctatatcta 5940

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc 6000

5941 gagtgatgcg ccgacgagtt tggacccgtc ttgcattcgg cgctctcgcg gttgttggcg 6000

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta 6060

6001 aagaaccagc ttccgtcgtt cgcgctactt acagaatgat gcctcgttca agggctccat 6060

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag 6120

6061 tagcctcagg ccgactacaa ccctcatcca ccgatgcaga ggcttgagtg ctggcttttc 6120

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat 6180

6121 tagttctcgt cgggcgtacc taaactgaac cagtcccggc tcggatgtac acgcttacta 6180

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt 6240

6181 cgggtatgaa ctcggtggat tgaaacaaaa tcccgctgac gggacgacgc attgtagcaa 6240

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 6300

6241 cgacgacgca ttgtagcaac gacgaggtat tgtagtttgt agctgggtgc cgcattgcgc 6300

gent .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga 6360

6301 gaacgacgaa cctacgggct ccgtatctga catgtttttt tgtcagtatt gttcggtact 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 6420

6361 tttggcggtg acgcggcaat ggtggcgacg caagccagtt ccaagacctg gtcaacgcac 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc 6480

6421 tcgcgtatgc gatgaacgta atgtcaaatg cttggcttgt ccgaatacag ttgacccaag 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 6540

6481 cacggaagta ggcaaaggtg ccacacgcag tgggccgttg gaacccgtcg tcgcttcagc 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 6600

6541 tccgtaaaga caggaccgac cgcttgctcg cgttccaaag ccagaggtgc gtagcagtcc 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc 6660

6601 gtaaccgccg gaacgacaag aagatgccgt tccacgacac gtgcctagac gggaccgaag 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctga.. .......... 6708

6661 tcctctagcc ttctggagcc ggcagcgccg cgaacggcca ccacgact.. .......... 6708

.......... .......... .......... .......... .......... ..........