Sequence of pDEST14

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pDEST14_Gateway

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pDEST14 Gateway : 6422 BP; 1620 A; 1568 C; 1698 G; 1536 T; 0 other;

BglII(1) .......... .......... .......... .......... XbaI(59)

1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc 60

1 tctagagcta gggcgcttta attatgctga gtgatatccc tctggtgttg ccaaagggag 60

.......... .......... T7_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tagatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat 120

61 atctagtgtt caaacatgtt ttttcgactt gctctttgca ttttactata tttatagtta 120

.......... .......... .......... attR1 attR2 ..........

.......... .......... .......... .......... .......... ..........

121 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc 180

121 tataatttaa tctaaaacgt attttttgtc tgatgtatta tgacattttg tgttgtatag 180

.......... ......... attR1 attR2 ......... .......... ..........

.......... NotI(193) .......... .......... .......... ..........

181 cagtcactat ggcggccgct aagttggcag catcacccga cgcactttgc gccgaataaa 240

181 gtcagtgata ccgccggcga ttcaaccgtc gtagtgggct gcgtgaaacg cggcttattt 240

attR1 attR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg 300

241 atggacactg ccttctagtg aagcgtctta tttatttagg accacaggga caactatggc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca 360

301 ccttcgggac ccggttgaaa accgctttta ctctgcaact agccgtgcat tctccaaggt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt 420

361 tgaaagtggt attactttat tctagtgatg gcccgcataa aaaactcaat agctctaaaa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 480

421 gtcctcgatt ccttcgattt tacctctttt tttagtgacc tatatggtgg caactatata 480

.......... .......... ........ ORF 3 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... ..........

481 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 540

481 gggttaccgt agcatttctt gtaaaactcc gtaaagtcag tcaacgagtt acatggatat 540

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 600

541 tggtctggca agtcgaccta taatgccgga aaaatttctg gcatttcttt ttattcgtgt 600

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc 660

601 tcaaaatagg ccggaaataa gtgtaagaac gggcggacta cttacgagta ggccttaagg 660

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 720

661 cataccgtta ctttctgcca ctcgaccact ataccctatc acaagtggga acaatgtggc 720

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 780

721 aaaaggtact cgtttgactt tgcaaaagta gcgagacctc acttatggtg ctgctaaagg 780

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 840

781 ccgtcaaaga tgtgtatata agcgttctac accgcacaat gccacttttg gaccggataa 840

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 900

841 agggatttcc caaataactc ttatacaaaa agcagagtcg gttagggacc cactcaaagt 900

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... NcoI(955)

901 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 960

901 ggtcaaaact aaatttgcac cggttatacc tgttgaagaa gcgggggcaa aagtggtacc 960

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 1020

961 cgtttataat atgcgttccg ctgttccacg actacggcga ccgctaagtc caagtagtac 1020

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 1080

1021 ggcagacact accgaaggta cagccgtctt acgaattact taatgttgtc atgacgctac 1080

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... ......... BamHI(1107) ......... .......... ..........

1081 agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg 1140

1081 tcaccgtccc gccccgcatt tgcgcaccta ggccgaatga ttttcggtct attgtcatac 1140

ORF 3 CAT/CamR ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta 1200

1141 gcataaacgc gcgactaaaa acgccatatt cttatatatg actatacata tgggcttcat 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta 1260

1201 acagtttttc tccacacgat acttcgtcgc ataatgtcac tgtcaactgt cgctgtcgat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag 1320

1261 agtcaacgag ttccgtatat actacagtta tagaggccag accattcgtg ttggtacgtc 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct 1380

1321 ttacttcggg cagcagacgc acggcttgcg acctttcgcc ttttagtcct tccctaccga 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag ggactggtga 1440

1381 ctccagcggg ccaaataact ttacttgccg agaaaacgac tgctcttgtc cctgaccact 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt 1500

1441 ttacgtcaaa ttccaaatgt ggatattttc tctctcggca atagcagaca aacacctaca 1500

.......... .......... .......... ccdB .......... ..........

.......... .......... SmaI(1525) .......... .......... ..........

1501 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg 1560

1501 tgtctcacta taataactgt gcgggcccgc tgcctaccac tagggggacc ggtcacgtgc 1560

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag 1620

1561 agacgacagt ctatttcaga gggcacttga aatgggccac cacgtatagc ccctactttc 1620

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt 1680

1621 gaccgcgtac tactggtggc tataccggtc acacggccag aggcaatagc cccttcttca 1680

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg 1740

1681 ccgactagag tcggtggcgc ttttactgta gtttttgcgg taattggact acaagacccc 1740

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... SalI(1782) ..........

1741 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga 1800

1741 ttatatttac agtccgaggg aatatgtgtc ggtcagacgt ccagctggta tcactgacct 1800

.......... .......... .......... .......... ......... attR2 attR1

.......... .......... .......... .......... .......... ..........

1801 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 1860

1801 atacaacaca aaatgtcata atacatcaga caaaaaatac gttttagatt aaattatata 1860

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatgatcc 1920

1861 actataaata tagtaaaatg caaagagcaa gtcgaaagaa catgtttcac cactactagg 1920

......... attR2 attR1 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact 1980

1921 ccgacgattg tttcgggctt tccttcgact caaccgacga cggtggcgac tcgttattga 1980

.......... .......... .......... T7_term ....... T7_Terminal_pri

.......... .......... .......... .......... .......... ..........

1981 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac 2040

1981 tcgtattggg gaaccccgga gatttgccca gaactcccca aaaaacgact ttcctccttg 2040

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc 2100

2041 atataggcct ataggtgtcc tgcccacacc agcggtacta gcgcatcagc tatcaccgag 2100

T7_term .......... ........ tet(900-1191) ........ ..........

.......... .......... .......... .......... .......... ..........

2101 caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa 2160

2101 gttcatcgct tcgctcgtcc tgacccgccg ccggtttcgc cagcctgtca cgaggctctt 2160

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... NheI(2196) .......... ..........

2161 cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg 2220

2161 gcccacgcgt atctttaacg tagttgcgta tatcgcgatc gtcgtgcggt atcactgacc 2220

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc 2280

2221 gctacgacag ccttacctgc tatagggcgt tctccgggcc gtcatggccg tattggttcg 2280

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt 2340

2281 gatacggatg tcgtaggtcc cactgccacg gctcctactg ctactcgcgt aacaatctaa 2340

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct 2400

2341 agtatgtgcc acggactgac gcaatcgtta aattgacact atttgatggc gtaatttcga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc 2460

2401 atagctacta ttcgacagtt tgtactctta agaacttctg ctttcccgga gcactatgcg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AatII(2506) .........

2461 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 2520

2461 gataaaaata tccaattaca gtactattat taccaaagaa tctgcagtcc accgtgaaaa 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 2580

2521 gcccctttac acgcgccttg gggataaaca aataaaaaga tttatgtaag tttatacata 2580

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

2581 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 2640

2581 ggcgagtact ctgttattgg gactatttac gaagttatta taactttttc cttctcatac 2640

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 2700

2641 tcataagttg taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa 2700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 2760

2701 aaacgagtgg gtctttgcga ccactttcat tttctacgac ttctagtcaa cccacgtgct 2760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 2820

2761 cacccaatgt agcttgacct agagttgtcg ccattctagg aactctcaaa agcggggctt 2820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 2880

2821 cttgcaaaag gttactactc gtgaaaattt caagacgata caccgcgcca taatagggca 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 2940

2881 caactgcggc ccgttctcgt tgagccagcg gcgtatgtga taagagtctt actgaaccaa 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 3000

2941 ctcatgagtg gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc tcttaatacg 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 3060

3001 tcacgacggt attggtactc actattgtga cgccggttga atgaagactg ttgctagcct 3060

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 3120

3061 cctggcttcc tcgattggcg aaaaaacgtg ttgtaccccc tagtacattg agcggaacta 3120

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 3180

3121 gcaacccttg gcctcgactt acttcggtat ggtttgctgc tcgcactgtg gtgctacgga 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 3240

3181 cgtcgttacc gttgttgcaa cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 3300

3241 gccgttgtta attatctgac ctacctccgc ctatttcaac gtcctggtga agacgcgagc 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 3360

3301 cgggaaggcc gaccgaccaa ataacgacta tttagacctc ggccactcgc acccagagcg 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 3420

3361 ccatagtaac gtcgtgaccc cggtctacca ttcgggaggg catagcatca atagatgtgc 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 3480

3421 tgcccctcag tccgttgata cctacttgct ttatctgtct agcgactcta tccacggagt 3480

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 3540

3481 gactaattcg taaccattga cagtctggtt caaatgagta tatatgaaat ctaactaaat 3540

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 3600

3541 tttgaagtaa aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 3660

3601 ttttagggaa ttgcactcaa aagcaaggtg actcgcagtc tggggcatct tttctagttt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 3720

3661 cctagaagaa ctctaggaaa aaaagacgcg cattagacga cgaacgtttg tttttttggt 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3780

3721 ggcgatggtc gccaccaaac aaacggccta gttctcgatg gttgagaaaa aggcttccat 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 3840

3781 tgaccgaagt cgtctcgcgt ctatggttta tgacaggaag atcacatcgg catcaatccg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 3900

3841 gtggtgaagt tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 3960

3901 caccgacgac ggtcaccgct attcagcaca gaatggccca acctgagttc tgctatcaat 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 4020

3961 ggcctattcc gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 4080

4021 gcttgctgga tgtggcttga ctctatggat gtcgcactcg atactctttc gcggtgcgaa 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 4140

4081 gggcttccct ctttccgcct gtccataggc cattcgccgt cccagccttg tcctctcgcg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 4200

4141 tgctccctcg aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 4260

4201 gagactgaac tcgcagctaa aaacactacg agcagtcccc ccgcctcgga tacctttttg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 4320

4261 cggtcgttgc gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg agtgtacaag 4320

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 4380

4321 aaaggacgca ataggggact aagacaccta ttggcataat ggcggaaact cactcgacta 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 4440

4381 tggcgagcgg cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct tcgccttctc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt 4500

4441 gcggactacg ccataaaaga ggaatgcgta gacacgccat aaagtgtggc gtatatacca 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc 4560

4501 cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcatatgt gaggcgatag 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg 4620

4561 cgatgcactg acccagtacc gacgcggggc tgtgggcggt tgtgggcgac tgcgcgggac 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 4680

4621 tgcccgaaca gacgagggcc gtaggcgaat gtctgttcga cactggcaga ggccctcgac 4680

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

4681 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc 4740

4681 gtacacagtc tccaaaagtg gcagtagtgg ctttgcgcgc tccgtcgacg ccatttcgag 4740

pGEX_3_pri .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt 4800

4741 tagtcgcacc agcacttcgc taagtgtcta cagacggaca agtaggcgca ggtcgagcaa 4800

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt 4860

4801 ctcaaagagg tcttcgcaat tacagaccga agactatttc gcccggtaca attcccgcca 4860

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat 4920

4861 aaaaaggaca aaccagtgac tacggaggca cattccccct aaagacaagt acccccatta 4920

ROP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg 4980

4921 ctatggctac tttgctctct cctacgagtg ctatgcccaa tgactactac ttgtacgggc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa 5040

4981 caatgacctt gcaacactcc catttgttga ccgccatacc tacgccgccc tggtctcttt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag 5100

5041 ttagtgagtc ccagttacgg tcgcgaagca attatgtcta catccacaag gtgtcccatc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt 5160

5101 ggtcgtcgta ggacgctacg tctaggcctt gtattaccac gtcccgcgac tgaaggcgca 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga 5220

5161 aaggtctgaa atgctttgtg cctttggctt ctggtaagta caacaacgag tccagcgtct 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc 5280

5221 gcaaaacgtc gtcgtcagcg aagtgcaagc gagcgcatag ccactaagta agacgattgg 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac 5340

5281 tcattccgtt ggggcggtcg gatcggccca ggagttgctg tcctcgtgct agtacgcgtg 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga 5400

5341 ggcaccggtc ctgggttgcg acgggctcta cgcggcgcac gccgacgacc tctaccgcct 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg 5460

5401 gcgctaccta tacaagacgg ttcccaacca aacgcgtaag tgtcaagagg cgttcttaac 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag 5520

5461 taaccgaggt taagaacctc accacttagg caatcgctcc acggcggccg aaggtaagtc 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg 5580

5521 cagctccacc gggccgaggt acgtggcgct gcgttgcgcc cctccgtctg ttccatatcc 5580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc 5640

5581 cgccgcggat gttaggtacg gttgggcaag gtacacgagc ggctccgccg tatttagcgg 5640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga 5700

5641 cactgctagt cgccaggtca ctagcttcaa tccgaccatt ctcggcgctc gctaggaact 5700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc 5760

5701 tcgacaggga ctaccagcag tagatggacg gacctgtcgt accggacgtt gcgcccgtag 5760

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... NruI(5818)

5761 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg 5820

5761 ggctacggcg gccttcgctc ttcttagtat taccccttcc ggtaggtcgg agcgcagcgc 5820

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc 5880

5821 ttgcggtcgt tctgcatcgg gtcgcgcagc cggcggtacg gccgctatta ccggacgaag 5880

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt 5940

5881 agcggctttg caaaccaccg ccctggtcac tgcttccgaa ctcgctcccg cacgttctaa 5940

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg 6000

5941 ggcttatggc gttcgctgtc cggctagtag cagcgcgagg tcgctttcgc caggagcggc 6000

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc 6060

6001 ttttactggg tctcgcgacg gccgtggaca ggatgctcaa cgtactattt cttctgtcag 6060

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag 6120

6061 tattcacgcc gctgctatca gtacggggcg cgggtggcct tcctcgactg acccaacttc 6120

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gctctcaagg gcatcggtcg atcgacgctc tcccttatgc gactcctgca ttaggaagca 6180

6121 cgagagttcc cgtagccagc tagctgcgag agggaatacg ctgaggacgt aatccttcgt 6180

ORF 3 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga 6240

6181 cgggtcatca tccaactccg gcaactcgtg gcggcggcgt tccttaccac gtacgttcct 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc 6300

6241 ctaccgcggg ttgtcagggg gccggtgccc cggacggtgg tatgggtgcg gctttgttcg 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc 6360

6301 cgagtactcg ggcttcaccg ctcgggctag aaggggtagc cactacagcc gctatatccg 6360

.......... .......... .......... ........ pBRrevBam_pri ........

.......... .......... .......... .......... .......... ..........

6361 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat 6420

6361 cggtcgttgg cgtggacacc gcggccacta cggccggtgc tacgcaggcc gcatctccta 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cg........ .......... .......... .......... .......... .......... 6422

6421 gc........ .......... .......... .......... .......... .......... 6422

.......... .......... .......... .......... .......... ..........