Sequence of pDEST15

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Sequence of pDEST15_Gateway

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Sequence of pDEST15 Gateway : 7013 BP; 1794 A; 1658 C; 1824 G; 1737 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atcgagatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc 60

1 tagctctaga gctagggcgc tttaattatg ctgagtgata tccctctggt gttgccaaag 60

.......... .......... .......... T7_pro .......... ..........

.......... .......... .......... .......... NdeI(103) ..........

61 cctctagaaa taattttgtt taactttaag aaggagatat acatatgtcc cctatactag 120

61 ggagatcttt attaaaacaa attgaaattc ttcctctata tgtatacagg ggatatgatc 120

.......... ....... T7_transl_en_RBS ....... .......... GST ORF 3

.......... .......... .......... .......... .......... ..........

121 gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag 180

121 caataacctt ttaattcccg gaacacgttg ggtgagctga agaaaacctt atagaacttc 180

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt 240

181 tttttatact tctcgtaaac atactcgcgc tacttccact atttaccgct ttgtttttca 240

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa 300

241 aacttaaccc aaacctcaaa gggttagaag gaataatata actaccacta caatttaatt 300

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc 360

301 gtgtcagata ccggtagtat gcaatatatc gactgttcgt gttgtacaac ccaccaacag 360

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg 420

361 gttttctcgc acgtctctaa agttacgaac ttcctcgcca aaacctataa tctatgccac 420

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc 480

421 aaagctctta acgtatatca tttctgaaac tttgagagtt tcaactaaaa gaatcgttcg 480

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... BstBI(502) .......... .......... BclI(539)

481 tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg 540

481 atggacttta cgacttttac aagcttctag caaatacagt attttgtata aatttaccac 540

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg 600

541 tagtacattg ggtaggactg aagtacaaca tactgcgaga actacaacaa aatatgtacc 600

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta 660

601 tgggttacac ggacctacgc aagggtttta atcaaacaaa attttttgca taacttcgat 660

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct 720

661 agggtgttta actattcatg aactttaggt cgttcatata tcgtaccgga aacgtcccga 720

.......... .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtccat 780

721 ccgttcggtg caaaccacca ccgctggtag gaggttttag cctagaccaa ggcgcaggta 780

pGEX_5_pri .......... GST ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggtcgaatca aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata 840

781 ccagcttagt ttgttcaaac atgttttttc gacttgctct ttgcatttta ctatatttat 840

.......... .......... ORF 3 attR1 attR2 ..........

.......... .......... .......... .......... .......... ..........

841 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac 900

841 agttatataa tttaatctaa aacgtatttt ttgtctgatg tattatgaca ttttgtgttg 900

.......... ......... attR1 attR2 ......... .......... ..........

.......... NotI(918) .......... .......... .......... ..........

901 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg 960

901 tataggtcag tgataccgcc ggcgtaatcc gtggggtccg aaatgtgaaa tacgaaggcc 960

attR1 attR2 ........ .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

961 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc 1020

961 gagcatatta cacacctaaa actcaatcct aggcagctct aaaagtcctc gattccttcg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa 1080

1021 attttacctc tttttttagt gacctatatg gtggcaacta tatagggtta ccgtagcatt 1080

.......... .......... ........ ORF 2 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... ..........

1081 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct 1140

1081 tcttgtaaaa ctccgtaaag tcagtcaacg agttacatgg atattggtct ggcaagtcga 1140

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt 1200

1141 cctataatgc cggaaaaatt tctggcattt ctttttattc gtgttcaaaa taggccggaa 1200

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga 1260

1201 ataagtgtaa gaacgggcgg actacttacg agtaggcctt aaggcatacc gttactttct 1260

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac 1320

1261 gccactcgac cactataccc tatcacaagt gggaacaatg tggcaaaagg tactcgtttg 1320

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat 1380

1321 actttgcaaa agtagcgaga cctcacttat ggtgctgcta aaggccgtca aagatgtgta 1380

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat 1440

1381 tataagcgtt ctacaccgca caatgccact tttggaccgg ataaagggat ttcccaaata 1440

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa 1500

1441 actcttatac aaaaagcaga gtcggttagg gacccactca aagtggtcaa aactaaattt 1500

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca 1560

1501 gcaccggtta tacctgttga agaagcgggg gcaaaagtgg tacccgttta taatatgcgt 1560

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt 1620

1561 tccgctgttc cacgactacg gcgaccgcta agtccaagta gtacggcaga cactaccgaa 1620

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc 1680

1621 ggtacagccg tcttacgaat tacttaatgt tgtcatgacg ctactcaccg tcccgccccg 1680

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtaatctaga ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga 1740

1681 cattagatct cctaggccga atgattttcg gtctattgtc atacgcataa acgcgcgact 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt 1800

1741 aaaaacgcca tattcttata tatgactata catatgggct tcatacagtt tttctccaca 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca 1860

1801 cgatacttcg tcgcataatg tcactgtcaa ctgtcgctgt cgatagtcaa cgagttccgt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc 1920

1861 atatactaca gttatagagg ccagaccatt cgtgttggta cgtcttactt cgggcagcag 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta 1980

1921 acgcacggct tgcgaccttt cgccttttag tccttcccta ccgactccag cgggccaaat 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttgaaatgaa cggctctttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt 2040

1981 aactttactt gccgagaaaa cgactgctct tgtccctgac cactttacgt caaattccaa 2040

.......... .......... .......... .......... .......... ccdB

.......... .......... .......... .......... .......... ..........

2041 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt 2100

2041 atgtggatat tttctctctc ggcaatagca gacaaacacc tacatgtctc actataataa 2100

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa 2160

2101 ctgtgcgggc ccgctgccta ccactagggg gaccggtcac gtgcagacga cagtctattt 2160

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc 2220

2161 cagagggcac ttgaaatggg ccaccacgta tagcccctac tttcgaccgc gtactactgg 2220

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac 2280

2221 tggctatacc ggtcacacgg ccagaggcaa tagccccttc ttcaccgact agagtcggtg 2280

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc 2340

2281 gcgcttttac tgtagttttt gcggtaattg gactacaaga ccccttatat ttacagtccg 2340

.......... .......... ccdB .......... .......... ..........

.......... .......... SalI(2366) .......... .......... ..........

2341 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca 2400

2341 agggaatatg tgtcggtcag acgtccagct ggtatcactg acctatacaa cacaaaatgt 2400

.......... .......... .......... ......... attR2 attR1 .........

.......... .......... .......... .......... .......... ..........

2401 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt 2460

2401 cataatacat cagacaaaaa atacgtttta gattaaatta tataactata aatatagtaa 2460

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttacgtttct cgttcagctt tcttgtacaa agtggtttga ttcgacccgg gatccggctg 2520

2461 aatgcaaaga gcaagtcgaa agaacatgtt tcaccaaact aagctgggcc ctaggccgac 2520

attR2 attR1 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat 2580

2521 gattgtttcg ggctttcctt cgactcaacc gacgacggtg gcgactcgtt attgatcgta 2580

.......... .......... T7_term ....... T7_Terminal_pri .......

.......... .......... .......... .......... .......... ..........

2581 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat 2640

2581 ttggggaacc ccggagattt gcccagaact ccccaaaaaa cgactttcct ccttgatata 2640

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt 2700

2641 ggcctatagg tgtcctgccc acaccagcgg tactagcgca tcagctatca ccgaggttca 2700

.......... .......... ........ tet(900-1191) ........ ..........

.......... .......... .......... .......... .......... ..........

2701 agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt 2760

2701 tcgcttcgct cgtcctgacc cgccgccggt ttcgccagcc tgtcacgagg ctcttgccca 2760

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... NheI(2791) .......... ..........

2761 gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg 2820

2761 cgcgtatctt taacgtagtt gcgtatatcg cgatcgtcgt gcggtatcac tgaccgctac 2820

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg 2880

2821 gacagcctta cctgctatag ggcgttctcc gggccgtcat ggccgtattg gttcggatac 2880

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata 2940

2881 ggatgtcgta ggtcccactg ccacggctcc tactgctact cgcgtaacaa tctaaagtat 2940

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... ........ HindIII(2991)

2941 cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg 3000

2941 gtgccacgga ctgacgcaat cgttaaattg acactatttg atggcgtaat ttcgaatagc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt 3060

3001 tactattcga cagtttgtac tcttaagaac ttctgctttc ccggagcact atgcggataa 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AatII(3101) .........

3061 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 3120

3061 aaatatccaa ttacagtact attattacca aagaatctgc agtccaccgt gaaaagcccc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3180

3121 tttacacgcg ccttggggat aaacaaataa aaagatttat gtaagtttat acataggcga 3180

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

3181 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 3240

3181 gtactctgtt attgggacta tttacgaagt tattataact ttttccttct catactcata 3240

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 3300

3241 agttgtaaag gcacagcggg aataagggaa aaaacgccgt aaaacggaag gacaaaaacg 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 3360

3301 agtgggtctt tgcgaccact ttcattttct acgacttcta gtcaacccac gtgctcaccc 3360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 3420

3361 aatgtagctt gacctagagt tgtcgccatt ctaggaactc tcaaaagcgg ggcttcttgc 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga 3480

3421 aaaaggttac tactcgtgaa aatttcaaga cgatacaccg cgccataata gggcacaact 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 3540

3481 gcggcccgtt ctcgttgagc cagcggcgta tgtgataaga gtcttactga accaactcat 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 3600

3541 gagtggtcag tgtcttttcg tagaatgcct accgtactgt cattctctta atacgtcacg 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 3660

3601 acggtattgg tactcactat tgtgacgccg gttgaatgaa gactgttgct agcctcctgg 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 3720

3661 cttcctcgat tggcgaaaaa acgtgttgta ccccctagta cattgagcgg aactagcaac 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc 3780

3721 ccttggcctc gacttacttc ggtatggttt gctgctcgca ctgtggtgct acggacgtcg 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 3840

3781 ttaccgttgt tgcaacgcgt ttgataattg accgcttgat gaatgagatc gaagggccgt 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 3900

3841 tgttaattat ctgacctacc tccgcctatt tcaacgtcct ggtgaagacg cgagccggga 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 3960

3901 aggccgaccg accaaataac gactatttag acctcggcca ctcgcaccca gagcgccata 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 4020

3961 gtaacgtcgt gaccccggtc taccattcgg gagggcatag catcaataga tgtgctgccc 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 4080

4021 ctcagtccgt tgatacctac ttgctttatc tgtctagcga ctctatccac ggagtgacta 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 4140

4081 attcgtaacc attgacagtc tggttcaaat gagtatatat gaaatctaac taaattttga 4140

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 4200

4141 agtaaaaatt aaattttcct agatccactt ctaggaaaaa ctattagagt actggtttta 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 4260

4201 gggaattgca ctcaaaagca aggtgactcg cagtctgggg catcttttct agtttcctag 4260

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4261 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 4320

4261 aagaactcta ggaaaaaaag acgcgcatta gacgacgaac gtttgttttt ttggtggcga 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 4380

4321 tggtcgccac caaacaaacg gcctagttct cgatggttga gaaaaaggct tccattgacc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 4440

4381 gaagtcgtct cgcgtctatg gtttatgaca ggaagatcac atcggcatca atccggtggt 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4500

4441 gaagttcttg agacatcgtg gcggatgtat ggagcgagac gattaggaca atggtcaccg 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4560

4501 acgacggtca ccgctattca gcacagaatg gcccaacctg agttctgcta tcaatggcct 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4620

4561 attccgcgtc gccagcccga cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 4680

4621 ctggatgtgg cttgactcta tggatgtcgc actcgatact ctttcgcggt gcgaagggct 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 4740

4681 tccctctttc cgcctgtcca taggccattc gccgtcccag ccttgtcctc tcgcgtgctc 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4800

4741 cctcgaaggt ccccctttgc ggaccataga aatatcagga cagcccaaag cggtggagac 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4860

4801 tgaactcgca gctaaaaaca ctacgagcag tccccccgcc tcggatacct ttttgcggtc 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 4920

4861 gttgcgccgg aaaaatgcca aggaccggaa aacgaccgga aaacgagtgt acaagaaagg 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 4980

4921 acgcaatagg ggactaagac acctattggc ataatggcgg aaactcactc gactatggcg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct 5040

4981 agcggcgtcg gcttgctggc tcgcgtcgct cagtcactcg ctccttcgcc ttctcgcgga 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact 5100

5041 ctacgccata aaagaggaat gcgtagacac gccataaagt gtggcgtata taccacgtga 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac 5160

5101 gagtcatgtt agacgagact acggcgtatc aattcggtca tatgtgaggc gatagcgatg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 5220

5161 cactgaccca gtaccgacgc ggggctgtgg gcggttgtgg gcgactgcgc gggactgccc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 5280

5221 gaacagacga gggccgtagg cgaatgtctg ttcgacactg gcagaggccc tcgacgtaca 5280

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

5281 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag 5340

5281 cagtctccaa aagtggcagt agtggctttg cgcgctccgt cgacgccatt tcgagtagtc 5340

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt 5400

5341 gcaccagcac ttcgctaagt gtctacagac ggacaagtag gcgcaggtcg agcaactcaa 5400

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt 5460

5401 agaggtcttc gcaattacag accgaagact atttcgcccg gtacaattcc cgccaaaaaa 5460

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac 5520

5461 ggacaaacca gtgactacgg aggcacattc cccctaaaga caagtacccc cattactatg 5520

ROP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac 5580

5521 gctactttgc tctctcctac gagtgctatg cccaatgact actacttgta cgggccaatg 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca 5640

5581 accttgcaac actcccattt gttgaccgcc atacctacgc cgccctggtc tctttttagt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc 5700

5641 gagtcccagt tacggtcgcg aagcaattat gtctacatcc acaaggtgtc ccatcggtcg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca 5760

5701 tcgtaggacg ctacgtctag gccttgtatt accacgtccc gcgactgaag gcgcaaaggt 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt 5820

5761 ctgaaatgct ttgtgccttt ggcttctggt aagtacaaca acgagtccag cgtctgcaaa 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa 5880

5821 acgtcgtcgt cagcgaagtg caagcgagcg catagccact aagtaagacg attggtcatt 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg 5940

5881 ccgttggggc ggtcggatcg gcccaggagt tgctgtcctc gtgctagtac gcgtgggcac 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga 6000

5941 cggtcctggg ttgcgacggg ctctacgcgg cgcacgccga cgacctctac cgcctgcgct 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg 6060

6001 acctatacaa gacggttccc aaccaaacgc gtaagtgtca agaggcgttc ttaactaacc 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga 6120

6061 gaggttaaga acctcaccac ttaggcaatc gctccacggc ggccgaaggt aagtccagct 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc 6180

6121 ccaccgggcc gaggtacgtg gcgctgcgtt gcgcccctcc gtctgttcca tatcccgccg 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac 6240

6181 cggatgttag gtacggttgg gcaaggtaca cgagcggctc cgccgtattt agcggcactg 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg 6300

6241 ctagtcgcca ggtcactagc ttcaatccga ccattctcgg cgctcgctag gaacttcgac 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat 6360

6301 agggactacc agcagtagat ggacggacct gtcgtaccgg acgttgcgcc cgtagggcta 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NruI(6413)

6361 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc 6420

6361 cggcggcctt cgctcttctt agtattaccc cttccggtag gtcggagcgc agcgcttgcg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc 6480

6421 gtcgttctgc atcgggtcgc gcagccggcg gtacggccgc tattaccgga cgaagagcgg 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa 6540

6481 ctttgcaaac caccgccctg gtcactgctt ccgaactcgc tcccgcacgt tctaaggctt 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat 6600

6541 atggcgttcg ctgtccggct agtagcagcg cgaggtcgct ttcgccagga gcggctttta 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag 6660

6601 ctgggtctcg cgacggccgt ggacaggatg ctcaacgtac tatttcttct gtcagtattc 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct 6720

6661 acgccgctgc tatcagtacg gggcgcgggt ggccttcctc gactgaccca acttccgaga 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 caagggcatc ggtcgatcga cgctctccct tatgcgactc ctgcattagg aagcagccca 6780

6721 gttcccgtag ccagctagct gcgagaggga atacgctgag gacgtaatcc ttcgtcgggt 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg 6840

6781 catcatccaa ctccggcaac tcgtggcggc ggcgttcctt accacgtacg ttcctctacc 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca 6900

6841 gcgggttgtc agggggccgg tgccccggac ggtggtatgg gtgcggcttt gttcgcgagt 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag 6960

6901 actcgggctt caccgctcgg gctagaaggg gtagccacta cagccgctat atccgcggtc 6960

.......... .......... .......... ........ pBRrevBam_pri ........

.......... .......... .......... .......... .......... ..........

6961 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag agg....... 7013

6961 gttggcgtgg acaccgcggc cactacggcc ggtgctacgc aggccgcatc tcc....... 7013

.......... .......... .......... .......... .......... ..........