ht4@ht4@+LOCUS pDEST17 Gateway 6354 bp DNA SYN 02-Sep-2007 DEFINITION pDEST17 Gateway ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6354 /organism="pDEST17 Gateway" /mol_type="other DNA" promoter 21..39 /label="T7_promoter" misc_feature 76..92 /label="T7_transl_en_RBS" misc_feature 140..264 /label="attR1" misc_feature complement(164..264) /label="attR2" promoter 289..312 /label="lac_promoter" CDS 373..1032 /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene 373..1032 /label="CAT/CamR" /gene="CAT/CamR" misc_feature 1374..1679 /label="ccdB" misc_feature 1720..1820 /label="attR2" misc_feature complement(1720..1827) /label="attR1" misc_feature complement(1897..1915) /label="T7_Terminal_primer" terminator 1855..1983 /label="T7_terminator" gene 1986..2276 /label="tet(900-1191)" /gene="tet(900-1191)" promoter 2500..2528 /label="AmpR_promoter" CDS 2570..3430 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 2570..3430 /label="Ampicillin" /gene="Ampicillin" rep_origin 3585..4204 /label="pBR322_origin" misc_feature 4603..4625 /label="pGEX_3_primer" misc_feature complement(4619..4810) /label="ROP" CDS complement(5449..6066) /label="ORF frame 1" /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" misc_feature 6272..6291 /label="pBRrevBam_primer" ORIGIN 1 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC 61 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGTCGTACT ACCATCACCA 121 TCACCATCAC CTCGAATCAA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA 181 TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA 241 AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT 301 GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT 361 AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG 421 CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC 481 GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT 541 CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA 601 ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT 661 GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT 721 CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA 781 GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT 841 GATTTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT 901 TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTCTGT 961 GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG 1021 GGCGGGGCGT AAAGATCTGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG 1081 CGCGCTGATT TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA 1141 AGAGGTGTGC TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC 1201 TCAAGGCATA TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC 1261 CCGTCGTCTG CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC 1321 CCGGTTTATT GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGACTGGT GAAATGCAGT 1381 TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG 1441 ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT 1501 CAGATAAAGT CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA 1561 TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC 1621 TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA 1681 TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT 1741 GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT 1801 ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTTGATTC GAGGCTGCTA 1861 ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC 1921 CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG 1981 GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC 2041 GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG 2101 CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG 2161 TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT 2221 ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC 2281 GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG 2341 ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT 2401 ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA 2461 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT 2521 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA 2581 ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA 2641 CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA 2701 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT 2761 TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC 2821 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC 2881 ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC 2941 CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA 3001 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA 3061 ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT 3121 GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA 3181 ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC 3241 GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT 3301 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG 3361 TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA 3421 GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA 3481 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC 3541 TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 3601 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 3661 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 3721 CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 3781 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 3841 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 3901 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 3961 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 4021 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 4081 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 4141 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 4201 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC 4261 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG 4321 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT 4381 GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC 4441 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG 4501 ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT 4561 GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC 4621 AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT 4681 GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT 4741 CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT 4801 GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA 4861 TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG 4921 AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC 4981 AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC 5041 ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC 5101 TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC 5161 AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC 5221 AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC 5281 AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG 5341 ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC 5401 CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT 5461 GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC 5521 TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT 5581 CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC 5641 CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC 5701 GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG 5761 CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA 5821 ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC 5881 CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC 5941 CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC 6001 GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA 6061 GGGCATCGGT CGATCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA 6121 GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC 6181 CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA 6241 GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA 6301 CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCG // pDEST17 Gateway "Times New Romanht4@C'T7_pro"Times New Romanht4@CL\T7_transl_en_RBS"Times New Romanht4@CɆattR1"Times New Romanht4@attR2"Times New Romanht4@C!8lac_pro"Times New Romanht4@CuORF 1"Times New Romanht4@CɆuCAT/CamR"Times New Romanht4@^ccdB"Times New Romanht4@CattR2"Times New Romanht4@C#attR1"Times New Romanht4@?T7_term"Times New Romanht4@CɆi{T7_Terminal_pri"Times New Romanht4@C tet(900-1191)"Times New Romanht4@C AmpR_pro"Times New Romanht4@CɆ f ORF 2"Times New Roman(ht4@ f  Ampicillin"Times New Romanht4@Cl pBR322_origin"Times New Romanht4@C pGEX_3_pri"Times New Romanht4@CɆ ROP"Times New Romanht4@IORF 1"Times New Romanht4@C pBRrevBam_pri"Times New Roman ht4@XbaI(59),NdeI(99)"Times New Roman;Zt[`OO1OO ht4@NotI"Times New Roman {hYJY ht4@NcoI"Times New Romanw V ht4@SmaI"Times New Roman2=F;Y8Y1? ht4@SalI"Times New Roman2vFxYzYs ht4@NheI"Times New RomanP$55u ht4@$HindIII(2328),ClaI(2335),AatII(2438)"Times New Roman a> ht4@NruI"Times New Romanvtet ht4@test"Times New Romanzfuucc  ht4@test"Times New RomanP+#jb;