Sequence of pDEST17

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pDEST17_Gateway

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pDEST17 Gateway : 6354 BP; 1601 A; 1549 C; 1673 G; 1531 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc 60

1 tctagagcta gggcgcttta attatgctga gtgatatccc tctggtgttg ccaaagggag 60

.......... .......... T7_pro .......... .......... ..........

.......... .......... .......... NdeI(99) .......... ..........

61 tagaaataat tttgtttaac tttaagaagg agatatacat atgtcgtact accatcacca 120

61 atctttatta aaacaaattg aaattcttcc tctatatgta tacagcatga tggtagtggt 120

.......... ....... T7_transl_en_RBS ....... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcaccatcac ctcgaatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga 180

121 agtggtagtg gagcttagtt gttcaaacat gttttttcga cttgctcttt gcattttact 180

.......... .......... .......... attR1 .......... attR2

.......... .......... .......... .......... .......... ..........

181 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa 240

181 atatttatag ttatataatt taatctaaaa cgtatttttt gtctgatgta ttatgacatt 240

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... NotI(266) .......... .......... ..........

241 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat 300

241 ttgtgttgta taggtcagtg ataccgccgg cgtaatccgt ggggtccgaa atgtgaaata 300

......... attR1 attR2 ......... .......... .......... lac_pro

.......... .......... .......... .......... .......... ..........

301 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct 360

301 cgaaggccga gcatattaca cacctaaaac tcaatcctag gcagctctaa aagtcctcga 360

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg 420

361 ttccttcgat tttacctctt tttttagtga cctatatggt ggcaactata tagggttacc 420

.......... .......... ........ ORF 1 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... ..........

421 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc 480

421 gtagcatttc ttgtaaaact ccgtaaagtc agtcaacgag ttacatggat attggtctgg 480

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat 540

481 caagtcgacc tataatgccg gaaaaatttc tggcatttct ttttattcgt gttcaaaata 540

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca 600

541 ggccggaaat aagtgtaaga acgggcggac tacttacgag taggccttaa ggcataccgt 600

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat 660

601 tactttctgc cactcgacca ctatacccta tcacaagtgg gaacaatgtg gcaaaaggta 660

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt 720

661 ctcgtttgac tttgcaaaag tagcgagacc tcacttatgg tgctgctaaa ggccgtcaaa 720

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa 780

721 gatgtgtata taagcgttct acaccgcaca atgccacttt tggaccggat aaagggattt 780

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt 840

781 cccaaataac tcttatacaa aaagcagagt cggttaggga cccactcaaa gtggtcaaaa 840

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... NcoI(887) ..........

841 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat 900

841 ctaaatttgc accggttata cctgttgaag aagcgggggc aaaagtggta cccgtttata 900

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt 960

901 atatgcgttc cgctgttcca cgactacggc gaccgctaag tccaagtagt acggcagaca 960

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag 1020

961 ctaccgaagg tacagccgtc ttacgaatta cttaatgttg tcatgacgct actcaccgtc 1020

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg 1080

1021 ccgccccgca tttctagacc taggccgaat gattttcggt ctattgtcat acgcataaac 1080

ORF 1 CAT/CamR ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa 1140

1081 gcgcgactaa aaacgccata ttcttatata tgactataca tatgggcttc atacagtttt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc 1200

1141 tctccacacg atacttcgtc gcataatgtc actgtcaact gtcgctgtcg atagtcaacg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc 1260

1201 agttccgtat atactacagt tatagaggcc agaccattcg tgttggtacg tcttacttcg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc 1320

1261 ggcagcagac gcacggcttg cgacctttcg ccttttagtc cttccctacc gactccagcg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt 1380

1321 ggccaaataa ctttacttgc cgagaaaacg actgctcttg tccctgacca ctttacgtca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg 1440

1381 aattccaaat gtggatattt tctctctcgg caatagcaga caaacaccta catgtctcac 1440

.......... .......... ccdB .......... .......... ..........

.......... SmaI(1457) .......... .......... .......... ..........

1441 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt 1500

1441 tataataact gtgcgggccc gctgcctacc actaggggga ccggtcacgt gcagacgaca 1500

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca 1560

1501 gtctatttca gagggcactt gaaatgggcc accacgtata gcccctactt tcgaccgcgt 1560

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc 1620

1561 actactggtg gctataccgg tcacacggcc agaggcaata gccccttctt caccgactag 1620

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa 1680

1621 agtcggtggc gcttttactg tagtttttgc ggtaattgga ctacaagacc ccttatattt 1680

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... SalI(1714) .......... ..........

1681 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt 1740

1681 acagtccgag ggaatatgtg tcggtcagac gtccagctgg tatcactgac ctatacaaca 1740

.......... .......... .......... ......... attR2 attR1 .........

.......... .......... .......... .......... .......... ..........

1741 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt 1800

1741 caaaatgtca taatacatca gacaaaaaat acgttttaga ttaaattata taactataaa 1800

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgattc gaggctgcta 1860

1801 tatagtaaaa tgcaaagagc aagtcgaaag aacatgtttc accaactaag ctccgacgat 1860

attR2 attR1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac 1920

1861 tgtttcgggc tttccttcga ctcaaccgac gacggtggcg actcgttatt gatcgtattg 1920

.......... .......... T7_term ....... T7_Terminal_pri .......

.......... .......... .......... .......... .......... ..........

1921 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg 1980

1921 gggaaccccg gagatttgcc cagaactccc caaaaaacga ctttcctcct tgatataggc 1980

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc 2040

1981 ctataggtgt cctgcccaca ccagcggtac tagcgcatca gctatcaccg aggttcatcg 2040

.......... .......... ........ tet(900-1191) ........ ..........

.......... .......... .......... .......... .......... ..........

2041 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg 2100

2041 cttcgctcgt cctgacccgc cgccggtttc gccagcctgt cacgaggctc ttgcccacgc 2100

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... NheI(2128) .......... .......... ..........

2101 catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg 2160

2101 gtatctttaa cgtagttgcg tatatcgcga tcgtcgtgcg gtatcactga ccgctacgac 2160

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct 2220

2161 agccttacct gctatagggc gttctccggg ccgtcatggc cgtattggtt cggatacgga 2220

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac 2280

2221 tgtcgtaggt cccactgcca cggctcctac tgctactcgc gtaacaatct aaagtatgtg 2280

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... ........ HindIII(2328) ........

2281 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg 2340

2281 ccacggactg acgcaatcgt taaattgaca ctatttgatg gcgtaatttc gaatagctac 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt 2400

2341 tattcgacag tttgtactct taagaacttc tgctttcccg gagcactatg cggataaaaa 2400

.......... .......... .......... .......... .......... ..........

.......... .......... ......... AatII(2438) ......... ..........

2401 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa 2460

2401 tatccaatta cagtactatt attaccaaag aatctgcagt ccaccgtgaa aagccccttt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 2520

2461 acacgcgcct tggggataaa caaataaaaa gatttatgta agtttataca taggcgagta 2520

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

2521 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 2580

2521 ctctgttatt gggactattt acgaagttat tataactttt tccttctcat actcataagt 2580

.......... .......... .......... .......... ....... ORF 2 Ampicillin

.......... .......... .......... .......... .......... ..........

2581 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 2640

2581 tgtaaaggca cagcgggaat aagggaaaaa acgccgtaaa acggaaggac aaaaacgagt 2640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 2700

2641 gggtctttgc gaccactttc attttctacg acttctagtc aacccacgtg ctcacccaat 2700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 2760

2701 gtagcttgac ctagagttgt cgccattcta ggaactctca aaagcggggc ttcttgcaaa 2760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc 2820

2761 aggttactac tcgtgaaaat ttcaagacga tacaccgcgc cataataggg cacaactgcg 2820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 2880

2821 gcccgttctc gttgagccag cggcgtatgt gataagagtc ttactgaacc aactcatgag 2880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 2940

2881 tggtcagtgt cttttcgtag aatgcctacc gtactgtcat tctcttaata cgtcacgacg 2940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 3000

2941 gtattggtac tcactattgt gacgccggtt gaatgaagac tgttgctagc ctcctggctt 3000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 3060

3001 cctcgattgg cgaaaaaacg tgttgtaccc cctagtacat tgagcggaac tagcaaccct 3060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat 3120

3061 tggcctcgac ttacttcggt atggtttgct gctcgcactg tggtgctacg gacgtcgtta 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 3180

3121 ccgttgttgc aacgcgtttg ataattgacc gcttgatgaa tgagatcgaa gggccgttgt 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 3240

3181 taattatctg acctacctcc gcctatttca acgtcctggt gaagacgcga gccgggaagg 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 3300

3241 ccgaccgacc aaataacgac tatttagacc tcggccactc gcacccagag cgccatagta 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 3360

3301 acgtcgtgac cccggtctac cattcgggag ggcatagcat caatagatgt gctgcccctc 3360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 3420

3361 agtccgttga tacctacttg ctttatctgt ctagcgactc tatccacgga gtgactaatt 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 3480

3421 cgtaaccatt gacagtctgg ttcaaatgag tatatatgaa atctaactaa attttgaagt 3480

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 3540

3481 aaaaattaaa ttttcctaga tccacttcta ggaaaaacta ttagagtact ggttttaggg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 3600

3541 aattgcactc aaaagcaagg tgactcgcag tctggggcat cttttctagt ttcctagaag 3600

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3601 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 3660

3601 aactctagga aaaaaagacg cgcattagac gacgaacgtt tgtttttttg gtggcgatgg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 3720

3661 tcgccaccaa acaaacggcc tagttctcga tggttgagaa aaaggcttcc attgaccgaa 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 3780

3721 gtcgtctcgc gtctatggtt tatgacagga agatcacatc ggcatcaatc cggtggtgaa 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3840

3781 gttcttgaga catcgtggcg gatgtatgga gcgagacgat taggacaatg gtcaccgacg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3900

3841 acggtcaccg ctattcagca cagaatggcc caacctgagt tctgctatca atggcctatt 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3960

3901 ccgcgtcgcc agcccgactt gccccccaag cacgtgtgtc gggtcgaacc tcgcttgctg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 4020

3961 gatgtggctt gactctatgg atgtcgcact cgatactctt tcgcggtgcg aagggcttcc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 4080

4021 ctctttccgc ctgtccatag gccattcgcc gtcccagcct tgtcctctcg cgtgctccct 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 4140

4081 cgaaggtccc cctttgcgga ccatagaaat atcaggacag cccaaagcgg tggagactga 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 4200

4141 actcgcagct aaaaacacta cgagcagtcc ccccgcctcg gatacctttt tgcggtcgtt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 4260

4201 gcgccggaaa aatgccaagg accggaaaac gaccggaaaa cgagtgtaca agaaaggacg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 4320

4261 caatagggga ctaagacacc tattggcata atggcggaaa ctcactcgac tatggcgagc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 4380

4321 ggcgtcggct tgctggctcg cgtcgctcag tcactcgctc cttcgccttc tcgcggacta 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc 4440

4381 cgccataaaa gaggaatgcg tagacacgcc ataaagtgtg gcgtatatac cacgtgagag 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg 4500

4441 tcatgttaga cgagactacg gcgtatcaat tcggtcatat gtgaggcgat agcgatgcac 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 4560

4501 tgacccagta ccgacgcggg gctgtgggcg gttgtgggcg actgcgcggg actgcccgaa 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 4620

4561 cagacgaggg ccgtaggcga atgtctgttc gacactggca gaggccctcg acgtacacag 4620

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

4621 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt 4680

4621 tctccaaaag tggcagtagt ggctttgcgc gctccgtcga cgccatttcg agtagtcgca 4680

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct 4740

4681 ccagcacttc gctaagtgtc tacagacgga caagtaggcg caggtcgagc aactcaaaga 4740

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct 4800

4741 ggtcttcgca attacagacc gaagactatt tcgcccggta caattcccgc caaaaaagga 4800

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga 4860

4801 caaaccagtg actacggagg cacattcccc ctaaagacaa gtacccccat tactatggct 4860

ROP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg 4920

4861 actttgctct ctcctacgag tgctatgccc aatgactact acttgtacgg gccaatgacc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc 4980

4921 ttgcaacact cccatttgtt gaccgccata cctacgccgc cctggtctct ttttagtgag 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc 5040

4981 tcccagttac ggtcgcgaag caattatgtc tacatccaca aggtgtccca tcggtcgtcg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac 5100

5041 taggacgcta cgtctaggcc ttgtattacc acgtcccgcg actgaaggcg caaaggtctg 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc 5160

5101 aaatgctttg tgcctttggc ttctggtaag tacaacaacg agtccagcgt ctgcaaaacg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc 5220

5161 tcgtcgtcag cgaagtgcaa gcgagcgcat agccactaag taagacgatt ggtcattccg 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc 5280

5221 ttggggcggt cggatcggcc caggagttgc tgtcctcgtg ctagtacgcg tgggcaccgg 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg 5340

5281 tcctgggttg cgacgggctc tacgcggcgc acgccgacga cctctaccgc ctgcgctacc 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc 5400

5341 tatacaagac ggttcccaac caaacgcgta agtgtcaaga ggcgttctta actaaccgag 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt 5460

5401 gttaagaacc tcaccactta ggcaatcgct ccacggcggc cgaaggtaag tccagctcca 5460

.......... .......... .......... .......... .......... ORF 1

.......... .......... .......... .......... .......... ..........

5461 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc 5520

5461 ccgggccgag gtacgtggcg ctgcgttgcg cccctccgtc tgttccatat cccgccgcgg 5520

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat 5580

5521 atgttaggta cggttgggca aggtacacga gcggctccgc cgtatttagc ggcactgcta 5580

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc 5640

5581 gtcgccaggt cactagcttc aatccgacca ttctcggcgc tcgctaggaa cttcgacagg 5640

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc 5700

5641 gactaccagc agtagatgga cggacctgtc gtaccggacg ttgcgcccgt agggctacgg 5700

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... NruI(5750) ..........

5701 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag 5760

5701 cggccttcgc tcttcttagt attacccctt ccggtaggtc ggagcgcagc gcttgcggtc 5760

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa 5820

5761 gttctgcatc gggtcgcgca gccggcggta cggccgctat taccggacga agagcggctt 5820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac 5880

5821 tgcaaaccac cgccctggtc actgcttccg aactcgctcc cgcacgttct aaggcttatg 5880

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac 5940

5881 gcgttcgctg tccggctagt agcagcgcga ggtcgctttc gccaggagcg gcttttactg 5940

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc 6000

5941 ggtctcgcga cggccgtgga caggatgctc aacgtactat ttcttctgtc agtattcacg 6000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa 6060

6001 ccgctgctat cagtacgggg cgcgggtggc cttcctcgac tgacccaact tccgagagtt 6060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gggcatcggt cgatcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta 6120

6061 cccgtagcca gctagctgcg agagggaata cgctgaggac gtaatccttc gtcgggtcat 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc 6180

6121 catccaactc cggcaactcg tggcggcggc gttccttacc acgtacgttc ctctaccgcg 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga 6240

6181 ggttgtcagg gggccggtgc cccggacggt ggtatgggtg cggctttgtt cgcgagtact 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa 6300

6241 cgggcttcac cgctcgggct agaaggggta gccactacag ccgctatatc cgcggtcgtt 6300

.......... .......... .......... ........ pBRrevBam_pri ........

.......... .......... .......... .......... .......... ..........

6301 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcg...... 6354

6301 ggcgtggaca ccgcggccac tacggccggt gctacgcagg ccgcatctcc tagc...... 6354

.......... .......... .......... .......... .......... ..........