jj/LOCUS pDEST20 7066 bp DNA SYN 24-Aug-2007 DEFINITION pDEST20 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7066 /organism="pDEST20" /mol_type="other DNA" misc_feature 43..60 /label="polyhedrin_fwd_primer" promoter 29..117 /label="PH_promoter" misc_feature 161..820 /label="GST" CDS 161..877 /label="ORF frame 2" /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK SDLVPRHNQTSLYKKAERET*" misc_feature 772..794 /label="pGEX_5_primer" misc_feature 842..966 /label="attR1" misc_feature complement(866..966) /label="attR2" promoter 991..1020 /label="lac_promoter" CDS 1075..1734 /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene 1075..1734 /label="CAT/CamR" /gene="CAT/CamR" misc_feature 2076..2381 /label="ccdB" misc_feature 2422..2522 /label="attR2" misc_feature complement(2422..2529) /label="attR1" terminator 2683..2814 /label="SV40_PA_terminator" misc_feature 2771..2790 /label="EBV_rev_primer" rep_origin 3208..3514 /label="f1_origin" promoter 3708..3736 /label="AmpR_promoter" CDS 3778..4638 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 3778..4638 /label="Ampicillin" /gene="Ampicillin" rep_origin 4793..5412 /label="pBR322_origin" misc_feature complement(5638..5697) /label="Tn7_att" gene complement(5991..6524) /label="gent" /gene="gent" ORIGIN 1 CTACGTATAC TCCGGAATAT TAATAGATCA TGGAGATAAT TAAAATGATA ACCATCTCGC 61 AAATAAATAA GTATTTTACT GTTTTCGTAA CAGTTTTGTA ATAAAAAAAC CTATAAATAT 121 TCCGGATTAT TCATACCGTC CCACCATCGG GCGCGGATCC ATGTCCCCTA TACTAGGTTA 181 TTGGAAAATT AAGGGCCTTG TGCAACCCAC TCGACTTCTT TTGGAATATC TTGAAGAAAA 241 ATATGAAGAG CATTTGTATG AGCGCGATGA AGGTGATAAA TGGCGAAACA AAAAGTTTGA 301 ATTGGGTTTG GAGTTTCCCA ATCTTCCTTA TTATATTGAT GGTGATGTTA AATTAACACA 361 GTCTATGGCC ATCATACGTT ATATAGCTGA CAAGCACAAC ATGTTGGGTG GTTGTCCAAA 421 AGAGCGTGCA GAGATTTCAA TGCTTGAAGG AGCGGTTTTG GATATTAGAT ACGGTGTTTC 481 GAGAATTGCA TATAGTAAAG ACTTTGAAAC TCTCAAAGTT GATTTTCTTA GCAAGCTACC 541 TGAAATGCTG AAAATGTTCG AAGATCGTTT ATGTCATAAA ACATATTTAA ATGGTGATCA 601 TGTAACCCAT CCTGACTTCA TGTTGTATGA CGCTCTTGAT GTTGTTTTAT ACATGGACCC 661 AATGTGCCTG GATGCGTTCC CAAAATTAGT TTGTTTTAAA AAACGTATTG AAGCTATCCC 721 ACAAATTGAT AAGTACTTGA AATCCAGCAA GTATATAGCA TGGCCTTTGC AGGGCTGGCA 781 AGCCACGTTT GGTGGTGGCG ACCATCCTCC AAAATCGGAT CTGGTTCCGC GTCATAATCA 841 AACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT 901 AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC 961 ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT 1021 GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG 1081 AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT 1141 GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG 1201 GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT 1261 CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG 1321 GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT 1381 TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA 1441 GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG 1501 TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT 1561 ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG 1621 GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTCT GTGATGGCTT CCATGTCGGC 1681 AGAATGCTTA ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAATCTAGA 1741 GGATCCGGCT TACTAAAAGC CAGATAACAG TATGCGTATT TGCGCGCTGA TTTTTGCGGT 1801 ATAAGAATAT ATACTGATAT GTATACCCGA AGTATGTCAA AAAGAGGTGT GCTATGAAGC 1861 AGCGTATTAC AGTGACAGTT GACAGCGACA GCTATCAGTT GCTCAAGGCA TATATGATGT 1921 CAATATCTCC GGTCTGGTAA GCACAACCAT GCAGAATGAA GCCCGTCGTC TGCGTGCCGA 1981 ACGCTGGAAA GCGGAAAATC AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA TTGAAATGAA 2041 CGGCTCTTTT GCTGACGAGA ACAGGGACTG GTGAAATGCA GTTTAAGGTT TACACCTATA 2101 AAAGAGAGAG CCGTTATCGT CTGTTTGTGG ATGTACAGAG TGATATTATT GACACGCCCG 2161 GGCGACGGAT GGTGATCCCC CTGGCCAGTG CACGTCTGCT GTCAGATAAA GTCTCCCGTG 2221 AACTTTACCC GGTGGTGCAT ATCGGGGATG AAAGCTGGCG CATGATGACC ACCGATATGG 2281 CCAGTGTGCC GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA TCTCAGCCAC CGCGAAAATG 2341 ACATCAAAAA CGCCATTAAC CTGATGTTCT GGGGAATATA AATGTCAGGC TCCCTTATAC 2401 ACAGCCAGTC TGCAGGTCGA CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA 2461 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT 2521 CGTTCAGCTT TCTTGTACAA AGTGGTTTGA TAGCTTGTCG AGAAGTACTA GAGGATCATA 2581 ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC 2641 CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT 2701 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG 2761 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCTGATCA 2821 CTGCTTGAGC CTAGGAGATC CGAACCAGAT AAGTGAAATC TAGTTCCAAA CTATTTTGTC 2881 ATTTTTAATT TTCGTATTAG CTTACGACGC TACACCCAGT TCCCATCTAT TTTGTCACTC 2941 TTCCCTAAAT AATCCTTAAA AACTCCATTT CCACCCCTCC CAGTTCCCAA CTATTTTGTC 3001 CGCCCACAGC GGGGCATTTT TCTTCCTGTT ATGTTTTTAA TCAAACATCC TGCCAACTCC 3061 ATGTGACAAA CCGTCATCTT CGGCTACTTT TTCTCTGTCA CAGAATGAAA ATTTTTCTGT 3121 CATCTCTTCG TTATTAATGT TTGTAATTGA CTGAATATCA ACGCTTATTT GCAGCCTGAA 3181 TGGCGAATGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 3241 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 3301 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 3361 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 3421 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 3481 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 3541 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 3601 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCAGG TGGCACTTTT 3661 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT 3721 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG 3781 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT 3841 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA 3901 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA 3961 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT 4021 ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT 4081 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC 4141 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA 4201 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT 4261 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT 4321 GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC 4381 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG 4441 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC 4501 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG 4561 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA 4621 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA 4681 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC 4741 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA 4801 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 4861 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA 4921 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC 4981 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA 5041 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA 5101 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 5161 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT 5221 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC 5281 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC 5341 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC 5401 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 5461 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT 5521 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG 5581 CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CAGACCAGCC 5641 GCGTAACCTG GCAAAATCGG TTACGGTTGA GTAATAAATG GATGCCCTGC GTAAGCGGGT 5701 GTGGGCGGAC AATAAAGTCT TAAACTGAAC AAAATAGATC TAAACTATGA CAATAAAGTC 5761 TTAAACTAGA CAGAATAGTT GTAAACTGAA ATCAGTCCAG TTATGCTGTG AAAAAGCATA 5821 CTGGACTTTT GTTATGGCTA AAGCAAACTC TTCATTTTCT GAAGTGCAAA TTGCCCGTCG 5881 TATTAAAGAG GGGCGTGGCC AAGGGCATGG TAAAGACTAT ATTCGCGGCG TTGTGACAAT 5941 TTACCGAACA ACTCCGCGGC CGGGAAGCCG ATCTCGGCTT GAACGAATTG TTAGGTGGCG 6001 GTACTTGGGT CGATATCAAA GTGCATCACT TCTTCCCGTA TGCCCAACTT TGTATAGAGA 6061 GCCACTGCGG GATCGTCACC GTAATCTGCT TGCACGTAGA TCACATAAGC ACCAAGCGCG 6121 TTGGCCTCAT GCTTGAGGAG ATTGATGAGC GCGGTGGCAA TGCCCTGCCT CCGGTGCTCG 6181 CCGGAGACTG CGAGATCATA GATATAGATC TCACTACGCG GCTGCTCAAA CCTGGGCAGA 6241 ACGTAAGCCG CGAGAGCGCC AACAACCGCT TCTTGGTCGA AGGCAGCAAG CGCGATGAAT 6301 GTCTTACTAC GGAGCAAGTT CCCGAGGTAA TCGGAGTCCG GCTGATGTTG GGAGTAGGTG 6361 GCTACGTCTC CGAACTCACG ACCGAAAAGA TCAAGAGCAG CCCGCATGGA TTTGACTTGG 6421 TCAGGGCCGA GCCTACATGT GCGAATGATG CCCATACTTG AGCCACCTAA CTTTGTTTTA 6481 GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTGCGTA ACATCGTTGC TGCTCCATAA 6541 CATCAAACAT CGACCCACGG CGTAACGCGC TTGCTGCTTG GATGCCCGAG GCATAGACTG 6601 TACAAAAAAA CAGTCATAAC AAGCCATGAA AACCGCCACT GCGCCGTTAC CACCGCTGCG 6661 TTCGGTCAAG GTTCTGGACC AGTTGCGTGA GCGCATACGC TACTTGCATT ACAGTTTACG 6721 AACCGAACAG GCTTATGTCA ACTGGGTTCG TGCCTTCATC CGTTTCCACG GTGTGCGTCA 6781 CCCGGCAACC TTGGGCAGCA GCGAAGTCGA GGCATTTCTG TCCTGGCTGG CGAACGAGCG 6841 CAAGGTTTCG GTCTCCACGC ATCGTCAGGC ATTGGCGGCC TTGCTGTTCT TCTACGGCAA 6901 GGTGCTGTGC ACGGATCTGC CCTGGCTTCA GGAGATCGGA AGACCTCGGC CGTCGCGGCG 6961 CTTGCCGGTG GTGCTGACCC CGGATGAAGT GGTTCGCATC CTCGGTTTTC TGGAAGGCGA 7021 GCATCGTTTG TTCGCCCAGG ACTCTAGCTA TAGTTCTAGT GGTTGG //XpDEST20 "Times New Roman$jCX+<polyhedrin_fwd_primer"Times New RomanjCXu PH_promoter"Times New RomanjCɆX4GST"Times New RomanjXmORF2Times New RomanjCX pGEX_5_primer"Times New Roman)jCXJattR1"Times New RomanjCɆXbattR2"Times New Roman)jX lac_promoter"Times New RomanjCX3ORF1"Times New RomanjCX3CAT/CamRTimes New RomanjCɆXM ccdB"Times New RomanjXv attR2"Times New RomanjCXv attR1"Times New RomanjCX{ SV40_PA_terminator"Times New RomanjCɆX EBV_rev_primer"Times New RomanjX  f1_origin"Times New RomanjCX| AmpR_promoter"Times New RomanjCX Orf frame 1"Times New RomanjCɆX Ampicillin"Times New RomanjX$ pBR322_origin"Times New RomanjCXATn7_att"Times New RomanjCXg|gent"Times New Roman jBstBI"Times New Roman.X&&o/]NN] jNotI"Times New Roman!$XVett jPvuII(1188), EcoRI(1288)"Times New Roman Xv jNcoI"Times New Roman5 X  jXbaI"Times New Roman X//.'5 jSmaI"Times New Romano Xz{ jPstI(2414), SalI(2416)"Times New Romanp $Xev jHpaI"Times New Romanw XHUbb jFspI"Times New Roman X5( jSacII"Times New RomanEX  jEcoRV"Times New Roman~ X" jtest"Times New RomanDXE& jtest"Times New Roman&XjVN ?]2 jtest"Times New Roman XVLA[A^