Sequence of pDEST20

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Sequence of pDEST20

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Sequence of pDEST20 : 7066 BP; 1856 A; 1606 C; 1662 G; 1942 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctacgtatac tccggaatat taatagatca tggagataat taaaatgata accatctcgc 60

1 gatgcatatg aggccttata attatctagt acctctatta attttactat tggtagagcg 60

.......... .......... .......... ... PH_promoter polyhedrin_fwd_primer

.......... .......... .......... .......... .......... ..........

61 aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat 120

61 tttatttatt cataaaatga caaaagcatt gtcaaaacat tatttttttg gatatttata 120

.......... ......... PH_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tccggattat tcataccgtc ccaccatcgg gcgcggatcc atgtccccta tactaggtta 180

121 aggcctaata agtatggcag ggtggtagcc cgcgcctagg tacaggggat atgatccaat 180

.......... .......... .......... .......... GST ORF2 ..........

.......... .......... .......... .......... .......... ..........

181 ttggaaaatt aagggccttg tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa 240

181 aaccttttaa ttcccggaac acgttgggtg agctgaagaa aaccttatag aacttctttt 240

.......... .......... GST ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atatgaagag catttgtatg agcgcgatga aggtgataaa tggcgaaaca aaaagtttga 300

241 tatacttctc gtaaacatac tcgcgctact tccactattt accgctttgt ttttcaaact 300

.......... .......... GST ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 attgggtttg gagtttccca atcttcctta ttatattgat ggtgatgtta aattaacaca 360

301 taacccaaac ctcaaagggt tagaaggaat aatataacta ccactacaat ttaattgtgt 360

.......... .......... GST ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gtctatggcc atcatacgtt atatagctga caagcacaac atgttgggtg gttgtccaaa 420

361 cagataccgg tagtatgcaa tatatcgact gttcgtgttg tacaacccac caacaggttt 420

.......... .......... GST ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agagcgtgca gagatttcaa tgcttgaagg agcggttttg gatattagat acggtgtttc 480

421 tctcgcacgt ctctaaagtt acgaacttcc tcgccaaaac ctataatcta tgccacaaag 480

.......... .......... GST ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gagaattgca tatagtaaag actttgaaac tctcaaagtt gattttctta gcaagctacc 540

481 ctcttaacgt atatcatttc tgaaactttg agagtttcaa ctaaaagaat cgttcgatgg 540

.......... .......... GST ORF2 .......... .......... ..........

.......... BstBI(558) .......... .......... .......... ..........

541 tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca 600

541 actttacgac ttttacaagc ttctagcaaa tacagtattt tgtataaatt taccactagt 600

.......... .......... GST ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc 660

601 acattgggta ggactgaagt acaacatact gcgagaacta caacaaaata tgtacctggg 660

.......... .......... GST ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc 720

661 ttacacggac ctacgcaagg gttttaatca aacaaaattt tttgcataac ttcgataggg 720

.......... .......... GST ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca 780

721 tgtttaacta ttcatgaact ttaggtcgtt catatatcgt accggaaacg tcccgaccgt 780

.......... .......... GST ORF2 .......... ........ pGEX_5_primer

.......... .......... .......... .......... .......... ..........

781 agccacgttt ggtggtggcg accatcctcc aaaatcggat ctggttccgc gtcataatca 840

781 tcggtgcaaa ccaccaccgc tggtaggagg ttttagccta gaccaaggcg cagtattagt 840

pGEX_5_primer GST ORF2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 900

841 ttgttcaaac atgttttttc gacttgctct ttgcatttta ctatatttat agttatataa 900

.......... ORF2 .......... attR1 attR2 ..........

.......... .......... .......... .......... .......... ..........

901 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 960

901 tttaatctaa aacgtatttt ttgtctgatg tattatgaca ttttgtgttg tataggtcag 960

.......... ......... attR1 attR2 ......... .......... ..........

NotI(968) .......... .......... .......... .......... ..........

961 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 1020

961 tgataccgcc ggcgtaatcc gtggggtccg aaatgtgaaa tacgaaggcc gagcatacaa 1020

.......... .......... .......... ......... lac_promoter .........

.......... .......... .......... .......... .......... ..........

1021 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag 1080

1021 cacacctaaa actcaatcct aggcagctct aaaagtcctc gattccttcg attttacctc 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 1140

1081 tttttttagt gacctatatg gtggcaacta tatagggtta ccgtagcatt tcttgtaaaa 1140

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 1200

1141 ctccgtaaag tcagtcaacg agttacatgg atattggtct ggcaagtcga cctataatgc 1200

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 1260

1201 cggaaaaatt tctggcattt ctttttattc gtgttcaaaa taggccggaa ataagtgtaa 1260

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... ......... EcoRI(1288) ......... .......... ..........

1261 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg 1320

1261 gaacgggcgg actacttacg agtaggcctt aaggcatacc gttactttct gccactcgac 1320

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 1380

1321 cactataccc tatcacaagt gggaacaatg tggcaaaagg tactcgtttg actttgcaaa 1380

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 1440

1381 agtagcgaga cctcacttat ggtgctgcta aaggccgtca aagatgtgta tataagcgtt 1440

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 1500

1441 ctacaccgca caatgccact tttggaccgg ataaagggat ttcccaaata actcttatac 1500

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 1560

1501 aaaaagcaga gtcggttagg gacccactca aagtggtcaa aactaaattt gcaccggtta 1560

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... NcoI(1589) .......... .......... ..........

1561 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag 1620

1561 tacctgttga agaagcgggg gcaaaagtgg tacccgttta taatatgcgt tccgctgttc 1620

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc 1680

1621 cacgactacg gcgaccgcta agtccaagta gtacggcaga cactaccgaa ggtacagccg 1680

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... XbaI(1735)

1681 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatctaga 1740

1681 tcttacgaat tacttaatgt tgtcatgacg ctactcaccg tcccgccccg cattagatct 1740

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 1800

1741 cctaggccga atgattttcg gtctattgtc atacgcataa acgcgcgact aaaaacgcca 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc 1860

1801 tattcttata tatgactata catatgggct tcatacagtt tttctccaca cgatacttcg 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 1920

1861 tcgcataatg tcactgtcaa ctgtcgctgt cgatagtcaa cgagttccgt atatactaca 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 1980

1921 gttatagagg ccagaccatt cgtgttggta cgtcttactt cgggcagcag acgcacggct 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 2040

1981 tgcgaccttt cgccttttag tccttcccta ccgactccag cgggccaaat aactttactt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cggctctttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata 2100

2041 gccgagaaaa cgactgctct tgtccctgac cactttacgt caaattccaa atgtggatat 2100

.......... .......... .......... .......... ccdB ..........

.......... .......... .......... .......... .......... SmaI(2159)

2101 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 2160

2101 tttctctctc ggcaatagca gacaaacacc tacatgtctc actataataa ctgtgcgggc 2160

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg 2220

2161 ccgctgccta ccactagggg gaccggtcac gtgcagacga cagtctattt cagagggcac 2220

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 2280

2221 ttgaaatggg ccaccacgta tagcccctac tttcgaccgc gtactactgg tggctatacc 2280

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 2340

2281 ggtcacacgg ccagaggcaa tagccccttc ttcaccgact agagtcggtg gcgcttttac 2340

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac 2400

2341 tgtagttttt gcggtaattg gactacaaga ccccttatat ttacagtccg agggaatatg 2400

.......... .......... ccdB .......... .......... ..........

.... PstI(2414),SalI(2416) .... .......... .......... ..........

2401 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 2460

2401 tgtcggtcag acgtccagct ggtatcactg acctatacaa cacaaaatgt cataatacat 2460

.......... .......... .......... ......... attR2 attR1 .........

.......... .......... .......... .......... .......... ..........

2461 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 2520

2461 cagacaaaaa atacgtttta gattaaatta tataactata aatatagtaa aatgcaaaga 2520

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgttcagctt tcttgtacaa agtggtttga tagcttgtcg agaagtacta gaggatcata 2580

2521 gcaagtcgaa agaacatgtt tcaccaaact atcgaacagc tcttcatgat ctcctagtat 2580

attR1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 2640

2581 tagtcggtat ggtgtaaaca tctccaaaat gaacgaaatt ttttggaggg tgtggagggg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... HpaI(2679) .......... ..........

2641 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 2700

2641 gacttggact ttgtatttta cttacgttaa caacaacaat tgaacaaata acgtcgaata 2700

.......... .......... .......... .......... ...... SV40_PA_terminator

.......... .......... .......... .......... .......... ..........

2701 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 2760

2701 ttaccaatgt ttatttcgtt atcgtagtgt ttaaagtgtt tatttcgtaa aaaaagtgac 2760

.......... ...... SV40_PA_terminator ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca 2820

2761 gtaagatcaa caccaaacag gtttgagtag ttacatagaa tagtacagac ctagactagt 2820

.... EBV_rev_primer SV40_PA_terminator .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctgcttgagc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc 2880

2821 gacgaactcg gatcctctag gcttggtcta ttcactttag atcaaggttt gataaaacag 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc 2940

2881 taaaaattaa aagcataatc gaatgctgcg atgtgggtca agggtagata aaacagtgag 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc 3000

2941 aagggattta ttaggaattt ttgaggtaaa ggtggggagg gtcaagggtt gataaaacag 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc 3060

3001 gcgggtgtcg ccccgtaaaa agaaggacaa tacaaaaatt agtttgtagg acggttgagg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt 3120

3061 tacactgttt ggcagtagaa gccgatgaaa aagagacagt gtcttacttt taaaaagaca 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa 3180

3121 gtagagaagc aataattaca aacattaact gacttatagt tgcgaataaa cgtcggactt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 3240

3181 accgcttacc tgcgcgggac atcgccgcgt aattcgcgcc gcccacacca ccaatgcgcg 3240

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

3241 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 3300

3241 tcgcactggc gatgtgaacg gtcgcgggat cgcgggcgag gaaagcgaaa gaagggaagg 3300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 3360

3301 aaagagcggt gcaagcggcc gaaaggggca gttcgagatt tagcccccga gggaaatccc 3360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 3420

3361 aaggctaaat cacgaaatgc cgtggagctg gggttttttg aactaatccc actaccaagt 3420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 3480

3421 gcatcacccg gtagcgggac tatctgccaa aaagcgggaa actgcaacct caggtgcaag 3480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 3540

3481 aaattatcac ctgagaacaa ggtttgacct tgttgtgagt tgggatagag ccagataaga 3540

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 3600

3541 aaactaaata ttccctaaaa cggctaaagc cggataacca attttttact cgactaaatt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt 3660

3601 gtttttaaat tgcgcttaaa attgttttat aattgcaaat gttaaagtcc accgtgaaaa 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 3720

3661 gcccctttac acgcgccttg gggataaaca aataaaaaga tttatgtaag tttatacata 3720

.......... .......... .......... .......... ........ AmpR_promoter

.......... .......... .......... .......... .......... ..........

3721 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 3780

3721 ggcgagtact ctgttattgg gactatttac gaagttatta taactttttc cttctcatac 3780

AmpR_promoter ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 3840

3781 tcataagttg taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa 3840

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 3900

3841 aaacgagtgg gtctttgcga ccactttcat tttctacgac ttctagtcaa cccacgtgct 3900

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 3960

3901 cacccaatgt agcttgacct agagttgtcg ccattctagg aactctcaaa agcggggctt 3960

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 4020

3961 cttgcaaaag gttactactc gtgaaaattt caagacgata caccgcgcca taatagggca 4020

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 4080

4021 taactgcggc ccgttctcgt tgagccagcg gcgtatgtga taagagtctt actgaaccaa 4080

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 4140

4081 ctcatgagtg gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc tcttaatacg 4140

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 4200

4141 tcacgacggt attggtactc actattgtga cgccggttga atgaagactg ttgctagcct 4200

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 4260

4201 cctggcttcc tcgattggcg aaaaaacgtg ttgtaccccc tagtacattg agcggaacta 4260

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 4320

4261 gcaacccttg gcctcgactt acttcggtat ggtttgctgc tcgcactgtg gtgctacgga 4320

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... FspI(4342) .......... .......... ..........

4321 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 4380

4321 catcgttacc gttgttgcaa cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg 4380

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 4440

4381 gccgttgtta attatctgac ctacctccgc ctatttcaac gtcctggtga agacgcgagc 4440

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 4500

4441 cgggaaggcc gaccgaccaa ataacgacta tttagacctc ggccactcgc acccagagcg 4500

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 4560

4501 ccatagtaac gtcgtgaccc cggtctacca ttcgggaggg catagcatca atagatgtgc 4560

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 4620

4561 tgcccctcag tccgttgata cctacttgct ttatctgtct agcgactcta tccacggagt 4620

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 4680

4621 gactaattcg taaccattga cagtctggtt caaatgagta tatatgaaat ctaactaaat 4680

Orf frame 1 Ampicillin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 4740

4681 tttgaagtaa aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 4800

4741 ttttagggaa ttgcactcaa aagcaaggtg actcgcagtc tggggcatct tttctagttt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 4860

4801 cctagaagaa ctctaggaaa aaaagacgcg cattagacga cgaacgtttg tttttttggt 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 4920

4861 ggcgatggtc gccaccaaac aaacggccta gttctcgatg gttgagaaaa aggcttccat 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 4980

4921 tgaccgaagt cgtctcgcgt ctatggttta tgacaggaag atcacatcgg catcaatccg 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 5040

4981 gtggtgaagt tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 5100

5041 caccgacgac ggtcaccgct attcagcaca gaatggccca acctgagttc tgctatcaat 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 5160

5101 ggcctattcc gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt 5220

5161 gcttgctgga tgtggcttga ctctatggat gtcgcactcg taactctttc gcggtgcgaa 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 5280

5221 gggcttccct ctttccgcct gtccataggc cattcgccgt cccagccttg tcctctcgcg 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 5340

5281 tgctccctcg aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 5400

5341 gagactgaac tcgcagctaa aaacactacg agcagtcccc ccgcctcgga tacctttttg 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 5460

5401 cggtcgttgc gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg agtgtacaag 5460

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 5520

5461 aaaggacgca ataggggact aagacaccta ttggcataat ggcggaaact cactcgacta 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 5580

5521 tggcgagcgg cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct tcgccttctc 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cagaccagcc 5640

5581 gcggactacg ccataaaaga ggaatgcgta gacacgccat aaagtgtggc gtctggtcgg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt 5700

5641 cgcattggac cgttttagcc aatgccaact cattatttac ctacgggacg cattcgccca 5700

.......... .......... Tn7_att .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc 5760

5701 cacccgcctg ttatttcaga atttgacttg ttttatctag atttgatact gttatttcag 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata 5820

5761 aatttgatct gtcttatcaa catttgactt tagtcaggtc aatacgacac tttttcgtat 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg 5880

5821 gacctgaaaa caataccgat ttcgtttgag aagtaaaaga cttcacgttt aacgggcagc 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tattaaagag gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat 5940

5881 ataatttctc cccgcaccgg ttcccgtacc atttctgata taagcgccgc aacactgtta 5940

.......... .......... .......... .......... .......... ..........

......... SacII(5957) ......... .......... .......... ..........

5941 ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg 6000

5941 aatggcttgt tgaggcgccg gcccttcggc tagagccgaa cttgcttaac aatccaccgc 6000

.......... .......... .......... .......... .......... gent

......... EcoRV(6014) ......... .......... .......... ..........

6001 gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga 6060

6001 catgaaccca gctatagttt cacgtagtga agaagggcat acgggttgaa acatatctct 6060

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg 6120

6061 cggtgacgcc ctagcagtgg cattagacga acgtgcatct agtgtattcg tggttcgcgc 6120

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg 6180

6121 aaccggagta cgaactcctc taactactcg cgccaccgtt acgggacgga ggccacgagc 6180

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa cctgggcaga 6240

6181 ggcctctgac gctctagtat ctatatctag agtgatgcgc cgacgagttt ggacccgtct 6240

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat 6300

6241 tgcattcggc gctctcgcgg ttgttggcga agaaccagct tccgtcgttc gcgctactta 6300

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg 6360

6301 cagaatgatg cctcgttcaa gggctccatt agcctcaggc cgactacaac cctcatccac 6360

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg 6420

6361 cgatgcagag gcttgagtgc tggcttttct agttctcgtc gggcgtacct aaactgaacc 6420

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta 6480

6421 agtcccggct cggatgtaca cgcttactac gggtatgaac tcggtggatt gaaacaaaat 6480

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa 6540

6481 cccgctgacg ggacgacgca ttgtagcaac gacgacgcat tgtagcaacg acgaggtatt 6540

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg 6600

6541 gtagtttgta gctgggtgcc gcattgcgcg aacgacgaac ctacgggctc cgtatctgac 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg 6660

6601 atgttttttt gtcagtattg ttcggtactt ttggcggtga cgcggcaatg gtggcgacgc 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg 6720

6661 aagccagttc caagacctgg tcaacgcact cgcgtatgcg atgaacgtaa tgtcaaatgc 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 aaccgaacag gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca 6780

6721 ttggcttgtc cgaatacagt tgacccaagc acggaagtag gcaaaggtgc cacacgcagt 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg 6840

6781 gggccgttgg aacccgtcgt cgcttcagct ccgtaaagac aggaccgacc gcttgctcgc 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 caaggtttcg gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa 6900

6841 gttccaaagc cagaggtgcg tagcagtccg taaccgccgg aacgacaaga agatgccgtt 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ggtgctgtgc acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg 6960

6901 ccacgacacg tgcctagacg ggaccgaagt cctctagcct tctggagccg gcagcgccgc 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga 7020

6961 gaacggccac cacgactggg gcctacttca ccaagcgtag gagccaaaag accttccgct 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gcatcgtttg ttcgcccagg actctagcta tagttctagt ggttgg.... .......... 7066

7021 cgtagcaaac aagcgggtcc tgagatcgat atcaagatca ccaacc.... .......... 7066

.......... .......... .......... .......... .......... ..........