Sequence of pDEST24

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Sequence of pDEST24_Gateway

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Sequence of pDEST24 Gateway : 6961 BP; 1773 A; 1652 C; 1818 G; 1718 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atcgagatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc 60

1 tagctctaga gctagggcgc tttaattatg ctgagtgata tccctctggt gttgccaaag 60

.......... .......... .......... T7_pro .......... ..........

XbaI(63) .......... .......... .......... .......... ..........

61 cctctagatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat 120

61 ggagatctag tgttcaaaca tgttttttcg acttgctctt tgcattttac tatatttata 120

.......... .......... .......... attR1 attR2 ..........

.......... .......... .......... .......... .......... ..........

121 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca 180

121 gttatataat ttaatctaaa acgtattttt tgtctgatgt attatgacat tttgtgttgt 180

.......... ......... attR1 attR2 ......... .......... ..........

.......... NotI(197) .......... .......... .......... ..........

181 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc 240

181 ataggtcagt gataccgccg gcgtaatccg tggggtccga aatgtgaaat acgaaggccg 240

attR1 attR2 ........ .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

241 tcgtataatg tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct 300

241 agcatattac acacctaaaa ctcaatccta ggccgctcta aaagtcctcg attccttcga 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa 360

301 ttttacctct ttttttagtg acctatatgg tggcaactat atagggttac cgtagcattt 360

.......... .......... ........ ORF 1 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... ..........

361 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg 420

361 cttgtaaaac tccgtaaagt cagtcaacga gttacatgga tattggtctg gcaagtcgac 420

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt 480

421 ctataatgcc ggaaaaattt ctggcatttc tttttattcg tgttcaaaat aggccggaaa 480

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac 540

481 taagtgtaag aacgggcgga ctacttacga gtaggcctta aggcataccg ttactttctg 540

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact 600

541 ccactcgacc actataccct atcacaagtg ggaacaatgt ggcaaaaggt actcgtttga 600

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata 660

601 ctttgcaaaa gtagcgagac ctcacttatg gtgctgctaa aggccgtcaa agatgtgtat 660

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt 720

661 ataagcgttc tacaccgcac aatgccactt ttggaccgga taaagggatt tcccaaataa 720

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac 780

721 ctcttataca aaaagcagag tcggttaggg acccactcaa agtggtcaaa actaaatttg 780

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa 840

781 caccggttat acctgttgaa gaagcggggg caaaagtggt acccgtttat aatatgcgtt 840

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc 900

841 ccgctgttcc acgactacgg cgaccgctaa gtccaagtag tacggcagac actaccgaag 900

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg 960

901 gtacagccgt cttacgaatt acttaatgtt gtcatgacgc tactcaccgt cccgccccgc 960

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat 1020

961 atttgcgcac ctaggccgaa tgattttcgg tctattgtca tacgcataaa cgcgcgacta 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg 1080

1021 aaaacgccat attcttatat atgactatac atatgggctt catacagttt ttctccacac 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat 1140

1081 gatacttcgt cgcataatgt cactgtcaac tgtcgctgtc gatagtcaac gagttccgta 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct 1200

1141 tatactacag ttatagaggc cagaccattc gtgttggtac gtcttacttc gggcagcaga 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat 1260

1201 cgcacggctt gcgacctttc gccttttagt ccttccctac cgactccagc gggccaaata 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt 1320

1261 actttacttg ccgagaaaac gactgctctt gtccctgacc actttacgtc aaattccaaa 1320

.......... .......... .......... .......... .......... ccdB

.......... .......... .......... .......... .......... ..........

1321 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg 1380

1321 tgtggatatt ttctctctcg gcaatagcag acaaacacct acatgtctca ctataataac 1380

.......... .......... ccdB .......... .......... ..........

SmaI(1388) .......... .......... .......... .......... ..........

1381 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag 1440

1381 tgtgcgggcc cgctgcctac cactaggggg accggtcacg tgcagacgac agtctatttc 1440

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca 1500

1441 agagggcact tgaaatgggc caccacgtat agcccctact ttcgaccgcg tactactggt 1500

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc 1560

1501 ggctataccg gtcacacggc cagaggcaat agccccttct tcaccgacta gagtcggtgg 1560

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct 1620

1561 cgcttttact gtagtttttg cggtaattgg actacaagac cccttatatt tacagtccga 1620

.......... .......... ccdB .......... .......... ..........

.......... .......... SalI(1645) .......... .......... ..........

1621 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag 1680

1621 gggaatatgt gtcggtcaga cgtccagctg gtatcactga cctatacaac acaaaatgtc 1680

.......... .......... .......... ......... attR2 attR1 .........

.......... .......... .......... .......... .......... ..........

1681 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt 1740

1681 ataatacatc agacaaaaaa tacgttttag attaaattat ataactataa atatagtaaa 1740

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tacgtttctc gttcagcttt cttgtacaaa gtggtgatta tgtcccctat actaggttat 1800

1741 atgcaaagag caagtcgaaa gaacatgttt caccactaat acaggggata tgatccaata 1800

attR2 attR1 ........ .......... .......... GST ORF 1 ..........

.......... .......... .......... .......... .......... ..........

1801 tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct tgaagaaaaa 1860

1801 accttttaat tcccggaaca cgttgggtga gctgaagaaa accttataga acttcttttt 1860

.......... .......... GST ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa 1920

1861 atacttctcg taaacatact cgcgctactt ccactattta ccgctttgtt tttcaaactt 1920

.......... .......... GST ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa attaacacag 1980

1921 aacccaaacc tcaaagggtt agaaggaata atataactac cactacaatt taattgtgtc 1980

.......... .......... GST ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa 2040

1981 agataccggt agtatgcaat atatcgactg ttcgtgttgt acaacccacc aacaggtttt 2040

.......... .......... GST ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata cggtgtttcg 2100

2041 ctcgcacgtc tctaaagtta cgaacttcct cgccaaaacc tataatctat gccacaaagc 2100

.......... .......... GST ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag caagctacct 2160

2101 tcttaacgta tatcatttct gaaactttga gagtttcaac taaaagaatc gttcgatgga 2160

.......... .......... GST ORF 1 .......... .......... ..........

......... BstBI(2177) ......... .......... .......... BclI(2214)

2161 gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa tggtgatcat 2220

2161 ctttacgact tttacaagct tctagcaaat acagtatttt gtataaattt accactagta 2220

.......... .......... GST ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata catggaccca 2280

2221 cattgggtag gactgaagta caacatactg cgagaactac aacaaaatat gtacctgggt 2280

.......... .......... GST ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga agctatccca 2340

2281 tacacggacc tacgcaaggg ttttaatcaa acaaaatttt ttgcataact tcgatagggt 2340

.......... .......... GST ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca gggctggcaa 2400

2341 gtttaactat tcatgaactt taggtcgttc atatatcgta ccggaaacgt cccgaccgtt 2400

.......... .......... GST ORF 1 .......... .......... pGEX_5_pri

.......... .......... .......... .......... .......... ..........

2401 gccacgtttg gtggtggcga ccatcctcca aaatcggatc tggttccgcg tccatgggga 2460

2401 cggtgcaaac caccaccgct ggtaggaggt tttagcctag accaaggcgc aggtacccct 2460

pGEX_5_pri GST ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata 2520

2461 aggccgacga ttgtttcggg ctttccttcg actcaaccga cgacggtggc gactcgttat 2520

ORF 1 .......... .......... T7_term ....... T7_Terminal_pri

.......... .......... .......... .......... .......... ..........

2521 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg 2580

2521 tgatcgtatt ggggaacccc ggagatttgc ccagaactcc ccaaaaaacg actttcctcc 2580

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg 2640

2581 ttgatatagg cctataggtg tcctgcccac accagcggta ctagcgcatc agctatcacc 2640

T7_term .......... ........ tet(900-1191) ........ ..........

.......... .......... .......... .......... .......... ..........

2641 ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga 2700

2641 gaggttcatc gcttcgctcg tcctgacccg ccgccggttt cgccagcctg tcacgaggct 2700

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... NheI(2739) .......... ..........

2701 gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac 2760

2701 cttgcccacg cgtatcttta acgtagttgc gtatatcgcg atcgtcgtgc ggtatcactg 2760

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca 2820

2761 accgctacga cagccttacc tgctataggg cgttctccgg gccgtcatgg ccgtattggt 2820

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag 2880

2821 tcggatacgg atgtcgtagg tcccactgcc acggctccta ctgctactcg cgtaacaatc 2880

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa 2940

2881 taaagtatgt gccacggact gacgcaatcg ttaaattgac actatttgat ggcgtaattt 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata 3000

2941 cgaatagcta ctattcgaca gtttgtactc ttaagaactt ctgctttccc ggagcactat 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AatII(3049) .........

3001 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 3060

3001 gcggataaaa atatccaatt acagtactat tattaccaaa gaatctgcag tccaccgtga 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 3120

3061 aaagcccctt tacacgcgcc ttggggataa acaaataaaa agatttatgt aagtttatac 3120

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

3121 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 3180

3121 ataggcgagt actctgttat tgggactatt tacgaagtta ttataacttt ttccttctca 3180

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 3240

3181 tactcataag ttgtaaaggc acagcgggaa taagggaaaa aacgccgtaa aacggaagga 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 3300

3241 caaaaacgag tgggtctttg cgaccacttt cattttctac gacttctagt caacccacgt 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 3360

3301 gctcacccaa tgtagcttga cctagagttg tcgccattct aggaactctc aaaagcgggg 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 3420

3361 cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg atacaccgcg ccataatagg 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 3480

3421 gcacaactgc ggcccgttct cgttgagcca gcggcgtatg tgataagagt cttactgaac 3480

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 3540

3481 caactcatga gtggtcagtg tcttttcgta gaatgcctac cgtactgtca ttctcttaat 3540

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 3600

3541 acgtcacgac ggtattggta ctcactattg tgacgccggt tgaatgaaga ctgttgctag 3600

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 3660

3601 cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc ccctagtaca ttgagcggaa 3660

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 3720

3661 ctagcaaccc ttggcctcga cttacttcgg tatggtttgc tgctcgcact gtggtgctac 3720

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 3780

3721 ggacgtcgtt accgttgttg caacgcgttt gataattgac cgcttgatga atgagatcga 3780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 3840

3781 agggccgttg ttaattatct gacctacctc cgcctatttc aacgtcctgg tgaagacgcg 3840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 3900

3841 agccgggaag gccgaccgac caaataacga ctatttagac ctcggccact cgcacccaga 3900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 3960

3901 gcgccatagt aacgtcgtga ccccggtcta ccattcggga gggcatagca tcaatagatg 3960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 4020

3961 tgctgcccct cagtccgttg atacctactt gctttatctg tctagcgact ctatccacgg 4020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 4080

4021 agtgactaat tcgtaaccat tgacagtctg gttcaaatga gtatatatga aatctaacta 4080

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 4140

4081 aattttgaag taaaaattaa attttcctag atccacttct aggaaaaact attagagtac 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 4200

4141 tggttttagg gaattgcact caaaagcaag gtgactcgca gtctggggca tcttttctag 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 4260

4201 tttcctagaa gaactctagg aaaaaaagac gcgcattaga cgacgaacgt ttgttttttt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 4320

4261 ggtggcgatg gtcgccacca aacaaacggc ctagttctcg atggttgaga aaaaggcttc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 4380

4321 cattgaccga agtcgtctcg cgtctatggt ttatgacagg aagatcacat cggcatcaat 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 4440

4381 ccggtggtga agttcttgag acatcgtggc ggatgtatgg agcgagacga ttaggacaat 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 4500

4441 ggtcaccgac gacggtcacc gctattcagc acagaatggc ccaacctgag ttctgctatc 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 4560

4501 aatggcctat tccgcgtcgc cagcccgact tgccccccaa gcacgtgtgt cgggtcgaac 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 4620

4561 ctcgcttgct ggatgtggct tgactctatg gatgtcgcac tcgatactct ttcgcggtgc 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 4680

4621 gaagggcttc cctctttccg cctgtccata ggccattcgc cgtcccagcc ttgtcctctc 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 4740

4681 gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa tatcaggaca gcccaaagcg 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 4800

4741 gtggagactg aactcgcagc taaaaacact acgagcagtc cccccgcctc ggataccttt 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 4860

4801 ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa cgaccggaaa acgagtgtac 4860

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 4920

4861 aagaaaggac gcaatagggg actaagacac ctattggcat aatggcggaa actcactcga 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 4980

4921 ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca gtcactcgct ccttcgcctt 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat 5040

4981 ctcgcggact acgccataaa agaggaatgc gtagacacgc cataaagtgt ggcgtatata 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct 5100

5041 ccacgtgaga gtcatgttag acgagactac ggcgtatcaa ttcggtcata tgtgaggcga 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc 5160

5101 tagcgatgca ctgacccagt accgacgcgg ggctgtgggc ggttgtgggc gactgcgcgg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 5220

5161 gactgcccga acagacgagg gccgtaggcg aatgtctgtt cgacactggc agaggccctc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag 5280

5221 gacgtacaca gtctccaaaa gtggcagtag tggctttgcg cgctccgtcg acgccatttc 5280

pGEX_3_pri .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc 5340

5281 gagtagtcgc accagcactt cgctaagtgt ctacagacgg acaagtaggc gcaggtcgag 5340

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc 5400

5341 caactcaaag aggtcttcgc aattacagac cgaagactat ttcgcccggt acaattcccg 5400

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt 5460

5401 ccaaaaaagg acaaaccagt gactacggag gcacattccc cctaaagaca agtaccccca 5460

.......... ROP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc 5520

5461 ttactatggc tactttgctc tctcctacga gtgctatgcc caatgactac tacttgtacg 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag 5580

5521 ggccaatgac cttgcaacac tcccatttgt tgaccgccat acctacgccg ccctggtctc 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg 5640

5581 tttttagtga gtcccagtta cggtcgcgaa gcaattatgt ctacatccac aaggtgtccc 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg 5700

5641 atcggtcgtc gtaggacgct acgtctaggc cttgtattac cacgtcccgc gactgaaggc 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc 5760

5701 gcaaaggtct gaaatgcttt gtgcctttgg cttctggtaa gtacaacaac gagtccagcg 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta 5820

5761 tctgcaaaac gtcgtcgtca gcgaagtgca agcgagcgca tagccactaa gtaagacgat 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg 5880

5821 tggtcattcc gttggggcgg tcggatcggc ccaggagttg ctgtcctcgt gctagtacgc 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc 5940

5881 gtgggcaccg gtcctgggtt gcgacgggct ctacgcggcg cacgccgacg acctctaccg 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa 6000

5941 cctgcgctac ctatacaaga cggttcccaa ccaaacgcgt aagtgtcaag aggcgttctt 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt 6060

6001 aactaaccga ggttaagaac ctcaccactt aggcaatcgc tccacggcgg ccgaaggtaa 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat 6120

6061 gtccagctcc accgggccga ggtacgtggc gctgcgttgc gcccctccgt ctgttccata 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc 6180

6121 tcccgccgcg gatgttaggt acggttgggc aaggtacacg agcggctccg ccgtatttag 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct 6240

6181 cggcactgct agtcgccagg tcactagctt caatccgacc attctcggcg ctcgctagga 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc 6300

6241 acttcgacag ggactaccag cagtagatgg acggacctgt cgtaccggac gttgcgcccg 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc 6360

6301 tagggctacg gcggccttcg ctcttcttag tattacccct tccggtaggt cggagcgcag 6360

.......... .......... .......... .......... .......... ..........

NruI(6361) .......... .......... .......... .......... ..........

6361 gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc 6420

6361 cgcttgcggt cgttctgcat cgggtcgcgc agccggcggt acggccgcta ttaccggacg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag 6480

6421 aagagcggct ttgcaaacca ccgccctggt cactgcttcc gaactcgctc ccgcacgttc 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg 6540

6481 taaggcttat ggcgttcgct gtccggctag tagcagcgcg aggtcgcttt cgccaggagc 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca 6600

6541 ggcttttact gggtctcgcg acggccgtgg acaggatgct caacgtacta tttcttctgt 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg 6660

6601 cagtattcac gccgctgcta tcagtacggg gcgcgggtgg ccttcctcga ctgacccaac 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 aaggctctca agggcatcgg tcgatcgacg ctctccctta tgcgactcct gcattaggaa 6720

6661 ttccgagagt tcccgtagcc agctagctgc gagagggaat acgctgagga cgtaatcctt 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa 6780

6721 cgtcgggtca tcatccaact ccggcaactc gtggcggcgg cgttccttac cacgtacgtt 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca 6840

6781 cctctaccgc gggttgtcag ggggccggtg ccccggacgg tggtatgggt gcggctttgt 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata 6900

6841 tcgcgagtac tcgggcttca ccgctcgggc tagaaggggt agccactaca gccgctatat 6900

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

6901 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag 6960

6901 ccgcggtcgt tggcgtggac accgcggcca ctacggccgg tgctacgcag gccgcatctc 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 g......... .......... .......... .......... .......... .......... 6961

6961 c......... .......... .......... .......... .......... .......... 6961

.......... .......... .......... .......... .......... ..........