Sequence of pDEST8

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Sequence of pDEST8

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Sequence of pDEST8 : 6526 BP; 1701 A; 1523 C; 1546 G; 1756 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa 60

1 gcatatgagg ccttataatt atctagtacc tctattaatt ttactattgg tagagcgttt 60

.......... .......... .......... .. polyhedrin_fwd_pri PH_pro ..

.......... .......... .......... .......... .......... ..........

61 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc 120

61 atttattcat aaaatgacaa aagcattgtc aaaacattat ttttttggat atttataagg 120

polyhedrin_fwd_pri .. PH_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggattattca taccgtccca ccatcgggcg cggatcatca caagtttgta caaaaaagct 180

121 cctaataagt atggcagggt ggtagcccgc gcctagtagt gttcaaacat gttttttcga 180

.......... .......... .......... .......... attR1 ..........

.......... .......... .......... .......... .......... ..........

181 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa 240

181 cttgctcttt gcattttact atatttatag ttatataatt taatctaaaa cgtatttttt 240

.......... .......... attR1 attR2 .......... ..........

.......... .......... .......... .......... NotI(286) ..........

241 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gctaagttgg 300

241 gtctgatgta ttatgacatt ttgtgttgta taggtcagtg ataccgccgg cgattcaacc 300

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cagcatcacc cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag 360

301 gtcgtagtgg gctgcgtgaa acgcggctta tttatggaca ctgccttcta gtgaagcgtc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa 420

361 ttatttattt aggaccacag ggacaactat ggcccttcgg gacccggttg aaaaccgctt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac 480

421 ttactctgca actagccgtg cattctccaa ggttgaaagt ggtattactt tattctagtg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga 540

481 atggcccgca taaaaaactc aatagctcta aaagtcctcg attccttcga ttttacctct 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 600

541 ttttttagtg acctatatgg tggcaactat atagggttac cgtagcattt cttgtaaaac 600

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 660

601 tccgtaaagt cagtcaacga gttacatgga tattggtctg gcaagtcgac ctataatgcc 660

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 720

661 ggaaaaattt ctggcatttc tttttattcg tgttcaaaat aggccggaaa taagtgtaag 720

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... EcoRI(747) .......... .......... ..........

721 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg 780

721 aacgggcgga ctacttacga gtaggcctta aggcataccg ttactttctg ccactcgacc 780

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 840

781 actataccct atcacaagtg ggaacaatgt ggcaaaaggt actcgtttga ctttgcaaaa 840

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 900

841 gtagcgagac ctcacttatg gtgctgctaa aggccgtcaa agatgtgtat ataagcgttc 900

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 960

901 tacaccgcac aatgccactt ttggaccgga taaagggatt tcccaaataa ctcttataca 960

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 1020

961 aaaagcagag tcggttaggg acccactcaa agtggtcaaa actaaatttg caccggttat 1020

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... NcoI(1048) .......... .......... ..........

1021 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 1080

1021 acctgttgaa gaagcggggg caaaagtggt acccgtttat aatatgcgtt ccgctgttcc 1080

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca 1140

1081 acgactacgg cgaccgctaa gtccaagtag tacggcagac actaccgaag gtacagccgt 1140

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... ......... BamHI(1200)

1141 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg 1200

1141 cttacgaatt acttaatgtt gtcatgacgc tactcaccgt cccgccccgc atttgcgcac 1200

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta 1260

1201 ctaggccgaa tgattttcgg tctattgtca tacgcataaa cgcgcgacta aaaacgccat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca 1320

1261 attcttatat atgactatac atatgggctt catacagttt ttctccacac gatacttcgt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc 1380

1321 cgcataatgt cactgtcaac tgtcgctgtc gatagtcaac gagttccgta tatactacag 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa 1440

1381 ttatagaggc cagaccattc gtgttggtac gtcttacttc gggcagcaga cgcacggctt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac 1500

1441 gcgacctttc gccttttagt ccttccctac cgactccagc gggccaaata actttacttg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa 1560

1501 ccgagaaaac gactgctctt gtccctgacc actttacgtc aaattccaaa tgtggatatt 1560

.......... .......... .......... .......... ccdB ..........

.......... .......... .......... .......... .......... SmaI(1618)

1561 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg 1620

1561 ttctctctcg gcaatagcag acaaacacct acatgtctca ctataataac tgtgcgggcc 1620

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga 1680

1621 cgctgcctac cactaggggg accggtcacg tgcagacgac agtctatttc agagggcact 1680

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc 1740

1681 tgaaatgggc caccacgtat agcccctact ttcgaccgcg tactactggt ggctataccg 1740

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga 1800

1741 gtcacacggc cagaggcaat agccccttct tcaccgacta gagtcggtgg cgcttttact 1800

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca 1860

1801 gtagtttttg cggtaattgg actacaagac cccttatatt tacagtccga gggaatatgt 1860

.......... ccdB .......... .......... .......... ..........

.... PstI(1873),SalI(1875) .... .......... .......... ..........

1861 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag 1920

1861 gtcggtcaga cgtccagctg gtatcactga cctatacaac acaaaatgtc ataatacatc 1920

.......... .......... ......... attR2 attR1 ......... ..........

.......... .......... .......... .......... .......... ..........

1921 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc 1980

1921 agacaaaaaa tacgttttag attaaattat ataactataa atatagtaaa atgcaaagag 1980

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gttcagcttt cttgtacaaa gtggtgatag cttgtcgaga agtactagag gatcataatc 2040

1981 caagtcgaaa gaacatgttt caccactatc gaacagctct tcatgatctc ctagtattag 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 2100

2041 tcggtatggt gtaaacatct ccaaaatgaa cgaaattttt tggagggtgt ggagggggac 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 2160

2101 ttggactttg tattttactt acgttaacaa caacaattga acaaataacg tcgaatatta 2160

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

2161 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 2220

2161 ccaatgttta tttcgttatc gtagtgttta aagtgtttat ttcgtaaaaa aagtgacgta 2220

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... BclI(2272)

2221 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg 2280

2221 agatcaacac caaacaggtt tgagtagtta catagaatag tacagaccta gactagtgac 2280

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cttgagccta ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt 2340

2281 gaactcggat cctctaggct tggtctattc actttagatc aaggtttgat aaaacagtaa 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tttaattttc gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc 2400

2341 aaattaaaag cataatcgaa tgctgcgatg tgggtcaagg gtagataaaa cagtgagaag 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc 2460

2401 ggatttatta ggaatttttg aggtaaaggt ggggagggtc aagggttgat aaaacaggcg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg 2520

2461 ggtgtcgccc cgtaaaaaga aggacaatac aaaaattagt ttgtaggacg gttgaggtac 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgacaaaccg tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat 2580

2521 actgtttggc agtagaagcc gatgaaaaag agacagtgtc ttacttttaa aaagacagta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctcttcgtta ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg 2640

2581 gagaagcaat aattacaaac attaactgac ttatagttgc gaataaacgt cggacttacc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 2700

2641 gcttacctgc gcgggacatc gccgcgtaat tcgcgccgcc cacaccacca atgcgcgtcg 2700

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

2701 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 2760

2701 cactggcgat gtgaacggtc gcgggatcgc gggcgaggaa agcgaaagaa gggaaggaaa 2760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 2820

2761 gagcggtgca agcggccgaa aggggcagtt cgagatttag cccccgaggg aaatcccaag 2820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 2880

2821 gctaaatcac gaaatgccgt ggagctgggg ttttttgaac taatcccact accaagtgca 2880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 2940

2881 tcacccggta gcgggactat ctgccaaaaa gcgggaaact gcaacctcag gtgcaagaaa 2940

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 3000

2941 ttatcacctg agaacaaggt ttgaccttgt tgtgagttgg gatagagcca gataagaaaa 3000

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 3060

3001 ctaaatattc cctaaaacgg ctaaagccgg ataaccaatt ttttactcga ctaaattgtt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcaggtgg cacttttcgg 3120

3061 tttaaattgc gcttaaaatt gttttataat tgcaaatgtt aaagtccacc gtgaaaagcc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 3180

3121 cctttacacg cgccttgggg ataaacaaat aaaaagattt atgtaagttt atacataggc 3180

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

3181 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 3240

3181 gagtactctg ttattgggac tatttacgaa gttattataa ctttttcctt ctcatactca 3240

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 3300

3241 taagttgtaa aggcacagcg ggaataaggg aaaaaacgcc gtaaaacgga aggacaaaaa 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 3360

3301 cgagtgggtc tttgcgacca ctttcatttt ctacgacttc tagtcaaccc acgtgctcac 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 3420

3361 ccaatgtagc ttgacctaga gttgtcgcca ttctaggaac tctcaaaagc ggggcttctt 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 3480

3421 gcaaaaggtt actactcgtg aaaatttcaa gacgatacac cgcgccataa tagggcataa 3480

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 3540

3481 ctgcggcccg ttctcgttga gccagcggcg tatgtgataa gagtcttact gaaccaactc 3540

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 3600

3541 atgagtggtc agtgtctttt cgtagaatgc ctaccgtact gtcattctct taatacgtca 3600

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 3660

3601 cgacggtatt ggtactcact attgtgacgc cggttgaatg aagactgttg ctagcctcct 3660

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 3720

3661 ggcttcctcg attggcgaaa aaacgtgttg taccccctag tacattgagc ggaactagca 3720

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 3780

3721 acccttggcc tcgacttact tcggtatggt ttgctgctcg cactgtggtg ctacggacat 3780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... FspI(3799) .......... .......... .......... ..........

3781 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 3840

3781 cgttaccgtt gttgcaacgc gtttgataat tgaccgcttg atgaatgaga tcgaagggcc 3840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 3900

3841 gttgttaatt atctgaccta cctccgccta tttcaacgtc ctggtgaaga cgcgagccgg 3900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 3960

3901 gaaggccgac cgaccaaata acgactattt agacctcggc cactcgcacc cagagcgcca 3960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 4020

3961 tagtaacgtc gtgaccccgg tctaccattc gggagggcat agcatcaata gatgtgctgc 4020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 4080

4021 ccctcagtcc gttgatacct acttgcttta tctgtctagc gactctatcc acggagtgac 4080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 4140

4081 taattcgtaa ccattgacag tctggttcaa atgagtatat atgaaatcta actaaatttt 4140

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 4200

4141 gaagtaaaaa ttaaattttc ctagatccac ttctaggaaa aactattaga gtactggttt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 4260

4201 tagggaattg cactcaaaag caaggtgact cgcagtctgg ggcatctttt ctagtttcct 4260

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4261 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 4320

4261 agaagaactc taggaaaaaa agacgcgcat tagacgacga acgtttgttt ttttggtggc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 4380

4321 gatggtcgcc accaaacaaa cggcctagtt ctcgatggtt gagaaaaagg cttccattga 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 4440

4381 ccgaagtcgt ctcgcgtcta tggtttatga caggaagatc acatcggcat caatccggtg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 4500

4441 gtgaagttct tgagacatcg tggcggatgt atggagcgag acgattagga caatggtcac 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 4560

4501 cgacgacggt caccgctatt cagcacagaa tggcccaacc tgagttctgc tatcaatggc 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 4620

4561 ctattccgcg tcgccagccc gacttgcccc ccaagcacgt gtgtcgggtc gaacctcgct 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc 4680

4621 tgctggatgt ggcttgactc tatggatgtc gcactcgtaa ctctttcgcg gtgcgaaggg 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 4740

4681 cttccctctt tccgcctgtc cataggccat tcgccgtccc agccttgtcc tctcgcgtgc 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 4800

4741 tccctcgaag gtcccccttt gcggaccata gaaatatcag gacagcccaa agcggtggag 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 4860

4801 actgaactcg cagctaaaaa cactacgagc agtccccccg cctcggatac ctttttgcgg 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 4920

4861 tcgttgcgcc ggaaaaatgc caaggaccgg aaaacgaccg gaaaacgagt gtacaagaaa 4920

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 4980

4921 ggacgcaata ggggactaag acacctattg gcataatggc ggaaactcac tcgactatgg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 5040

4981 cgagcggcgt cggcttgctg gctcgcgtcg ctcagtcact cgctccttcg ccttctcgcg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcag accagccgcg 5100

5041 gactacgcca taaaagagga atgcgtagac acgccataaa gtgtggcgtc tggtcggcgc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta agcgggtgtg 5160

5101 attggaccgt tttagccaat gccaactcat tatttaccta cgggacgcat tcgcccacac 5160

.......... .......... Tn7_att .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta 5220

5161 ccgcctgtta tttcagaatt tgacttgttt tatctagatt tgatactgtt atttcagaat 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg 5280

5221 ttgatctgtc ttatcaacat ttgactttag tcaggtcaat acgacacttt ttcgtatgac 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat 5340

5281 ctgaaaacaa taccgatttc gtttgagaag taaaagactt cacgtttaac gggcagcata 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 taaagagggg cgtggccaag ggcatggtaa agactatatt cgcggcgttg tgacaattta 5400

5341 atttctcccc gcaccggttc ccgtaccatt tctgatataa gcgccgcaac actgttaaat 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta 5460

5401 ggcttgttga ggcgccggcc cttcggctag agccgaactt gcttaacaat ccaccgccat 5460

.......... .......... .......... .......... .......... gent

......... EcoRV(5471) ......... .......... .......... ..........

5461 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc 5520

5461 gaacccagct atagtttcac gtagtgaaga agggcatacg ggttgaaaca tatctctcgg 5520

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg 5580

5521 tgacgcccta gcagtggcat tagacgaacg tgcatctagt gtattcgtgg ttcgcgcaac 5580

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg 5640

5581 cggagtacga actcctctaa ctactcgcgc caccgttacg ggacggaggc cacgagcggc 5640

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaacct gggcagaacg 5700

5641 ctctgacgct ctagtatcta tatctagagt gatgcgccga cgagtttgga cccgtcttgc 5700

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc 5760

5701 attcggcgct ctcgcggttg ttggcgaaga accagcttcc gtcgttcgcg ctacttacag 5760

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct 5820

5761 aatgatgcct cgttcaaggg ctccattagc ctcaggccga ctacaaccct catccaccga 5820

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca 5880

5821 tgcagaggct tgagtgctgg cttttctagt tctcgtcggg cgtacctaaa ctgaaccagt 5880

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg 5940

5881 cccggctcgg atgtacacgc ttactacggg tatgaactcg gtggattgaa acaaaatccc 5940

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat 6000

5941 gctgacggga cgacgcattg tagcaacgac gacgcattgt agcaacgacg aggtattgta 6000

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac 6060

6001 gtttgtagct gggtgccgca ttgcgcgaac gacgaaccta cgggctccgt atctgacatg 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc 6120

6061 tttttttgtc agtattgttc ggtacttttg gcggtgacgc ggcaatggtg gcgacgcaag 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac 6180

6121 ccagttccaa gacctggtca acgcactcgc gtatgcgatg aacgtaatgt caaatgcttg 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc 6240

6181 gcttgtccga atacagttga cccaagcacg gaagtaggca aaggtgccac acgcagtggg 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa 6300

6241 ccgttggaac ccgtcgtcgc ttcagctccg taaagacagg accgaccgct tgctcgcgtt 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt 6360

6301 ccaaagccag aggtgcgtag cagtccgtaa ccgccggaac gacaagaaga tgccgttcca 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt 6420

6361 cgacacgtgc ctagacggga ccgaagtcct ctagccttct ggagccggca gcgccgcgaa 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca 6480

6421 cggccaccac gactggggcc tacttcacca agcgtaggag ccaaaagacc ttccgctcgt 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tcgtttgttc gcccaggact ctagctatag ttctagtggt tggcta.... .......... 6526

6481 agcaaacaag cgggtcctga gatcgatatc aagatcacca accgat.... .......... 6526

.......... .......... .......... .......... .......... ..........