OsOs#LOCUS pDNR-1r 4919 bp DNA SYN 26-Aug-2007 DEFINITION pDNR-1r ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4919 /organism="pDNR-1r" /mol_type="other DNA" misc_feature 9..42 /label="loxP" misc_feature complement(144..162) /label="M13_reverse_primer" gene complement(308..967) /label="CAT/CamR" /gene="CAT/CamR" CDS complement(308..1012) /label="ORF frame 2" /translation="MLYEVMDLSGAKEAKMEKKITGYTTVDISQWHRKEHFEAFQSVA QCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDS VHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFV SANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGR MLNELQQYCDEWQGGA*" misc_feature 996..1029 /label="loxP" gene 1038..2948 /label="sacB_neg" /gene="sacB_neg" CDS 1490..2911 /label="ORF frame 2" /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS HITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHG YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN IKGKKTSVVKDSILEQGQLTVNK*" promoter 3014..3042 /label="AmpR_promoter" CDS 3084..3944 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 3084..3944 /label="Ampicillin" /gene="Ampicillin" rep_origin 4099..4718 /label="pBR322_origin" misc_feature 4820..4836 /label="M13_forward20_primer" promoter 4844..4862 /label="T7_promoter" ORIGIN 1 GCGGCCGCAT AACTTCGTAT AGCATACATT ATACGAAGTT ATCAGTCGAC GGTACCGGAC 61 ATATGCCCGG GAATTCCTGC AGGATCCGCT CGAGAAGCTT TCTAGACCAT TCGTTTGGCG 121 CGCGGGCCCA GTAGGTAAGT GAACATGGTC ATAGCTGTTT CCTAGGAGAT CCTGGTCATG 181 ACTAGTGCTT GGATTCTCAC CAATAAAAAA CGCCCGGCGG CAACCGAGCG TTCTGAACAA 241 ATCCAGATGG AGTTCTGAGG TCATTACTGG ATCTATCAAC AGGAGTCCAA GCGAGCTCGA 301 TATCAAATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC 361 TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA 421 CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA 481 TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA 541 ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT 601 CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG 661 AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA 721 CCAGCTCACC GTCTTTCATT GCCATACGAA ATTCCGGATG AGCATTCATC AGGCGGGCAA 781 GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG 841 CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT 901 CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT 961 TCTCCATTTT AGCTTCCTTA GCTCCTGAAA GATCCATAAC TTCGTATAGC ATACATTATA 1021 CGAAGTTATG CGGCCGCGAC GTCCACATAT ACCTGCCGTT CACTATTATT TAGTGAAATG 1081 AGATATTATG ATATTTTCTG AATTGTGATT AAAAAGGCAA CTTTATGCCC ATGCAACAGA 1141 AACTATAAAA AATACAGAGA ATGAAAAGAA ACAGATAGAT TTTTTAGTTC TTTAGGCCCG 1201 TAGTCTGCAA ATCCTTTTAT GATTTTCTAT CAAACAAAAG AGGAAAATAG ACCAGTTGCA 1261 ATCCAAACGA GAGTCTAATA GAATGAGGTC GAAAAGTAAA TCGCGCGGGT TTGTTACTGA 1321 TAAAGCAGGC AAGACCTAAA ATGTGTAAAG GGCAAAGTGT ATACTTTGGC GTCACCCCTT 1381 ACATATTTTA GGTCTTTTTT TATTGTGCGT AACTAACTTG CCATCTTCAA ACAGGAGGGC 1441 TGGAAGAAGC AGACCGCTAA CACAGTACAT AAAAAAGGAG ACATGAACGA TGAACATCAA 1501 AAAGTTTGCA AAACAAGCAA CAGTATTAAC CTTTACTACC GCACTGCTGG CAGGAGGCGC 1561 AACTCAAGCG TTTGCGAAAG AAACGAACCA AAAGCCATAT AAGGAAACAT ACGGCATTTC 1621 CCATATTACA CGCCATGATA TGCTGCAAAT CCCTGAACAG CAAAAAAATG AAAAATATCA 1681 AGTTCCTGAG TTCGATTCGT CCACAATTAA AAATATCTCT TCTGCAAAAG GCCTGGACGT 1741 TTGGGACAGC TGGCCATTAC AAAACGCTGA CGGCACTGTC GCAAACTATC ACGGCTACCA 1801 CATCGTCTTT GCATTAGCCG GAGATCCTAA AAATGCGGAT GACACATCGA TTTACATGTT 1861 CTATCAAAAA GTCGGCGAAA CTTCTATTGA CAGCTGGAAA AACGCTGGCC GCGTCTTTAA 1921 AGACAGCGAC AAATTCGATG CAAATGATTC TATCCTAAAA GACCAAACAC AAGAATGGTC 1981 AGGTTCAGCC ACATTTACAT CTGACGGAAA AATCCGTTTA TTCTACACTG ATTTCTCCGG 2041 TAAACATTAC GGCAAACAAA CACTGACAAC TGCACAAGTT AACGTATCAG CATCAGACAG 2101 CTCTTTGAAC ATCAACGGTG TAGAGGATTA TAAATCAATC TTTGACGGTG ACGGAAAAAC 2161 GTATCAAAAT GTACAGCAGT TCATCGATGA AGGCAACTAC AGCTCAGGCG ACAACCATAC 2221 GCTGAGAGAT CCTCACTACG TAGAAGATAA AGGCCACAAA TACTTAGTAT TTGAAGCAAA 2281 CACTGGAACT GAAGATGGCT ACCAAGGCGA AGAATCTTTA TTTAACAAAG CATACTATGG 2341 CAAAAGCACA TCATTCTTCC GTCAAGAAAG TCAAAAACTT CTGCAAAGCG ATAAAAAACG 2401 CACGGCTGAG TTAGCAAACG GCGCTCTCGG TATGATTGAG CTAAACGATG ATTACACACT 2461 GAAAAAAGTG ATGAAACCGC TGATTGCATC TAACACAGTA ACAGATGAAA TTGAACGCGC 2521 GAACGTCTTT AAAATGAACG GCAAATGGTA CCTGTTCACT GACTCCCGCG GATCAAAAAT 2581 GACGATTGAC GGCATTACGT CTAACGATAT TTACATGCTT GGTTATGTTT CTAATTCTTT 2641 AACTGGCCCA TACAAGCCGC TGAACAAAAC TGGCCTTGTG TTAAAAATGG ATCTTGATCC 2701 TAACGATGTA ACCTTTACTT ACTCACACTT CGCTGTACCT CAAGCGAAAG GAAACAATGT 2761 CGTGATTACA AGCTATATGA CAAACAGAGG ATTCTACGCA GACAAACAAT CAACGTTTGC 2821 GCCTAGCTTC CTGCTGAACA TCAAAGGCAA GAAAACATCT GTTGTCAAAG ACAGCATCCT 2881 TGAACAAGGA CAATTAACAG TTAACAAATA AAAACGCAAA AGAAAATGCC GATATCCTAT 2941 TGGCATTGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 3001 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 3061 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 3121 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 3181 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 3241 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 3301 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 3361 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 3421 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 3481 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 3541 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 3601 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 3661 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 3721 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 3781 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 3841 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 3901 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 3961 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 4021 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 4081 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 4141 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 4201 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 4261 TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 4321 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 4381 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 4441 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 4501 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 4561 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 4621 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 4681 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 4741 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 4801 GTATTACCGC CTTACGCGTG TAAAACGACG GCCAGTAGAT CTGTAATACG ACTCACTATA 4861 GGGCGCTAGC TGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAA //7 pDNR-1r "Times New RomanOsC$ *loxP"Times New RomanOsC$M13_reverse_pri"Times New RomanOsCɆ$4CAT/CamR"Times New Roman(Os$4ORF 2"Times New RomanOsC$loxP"Times New RomanOsC$ sacB_neg"Times New Roman(OsCɆ$_ ORF 2"Times New RomanOs$ AmpR_pro"Times New RomanOsC$ hORF 3"Times New RomanOsC$ h Ampicillin"Times New Roman(OsCɆ$n pBR322_origin"Times New RomanOs$M13_forward20_pri"Times New RomanOsC$T7_pro"Times New Roman OsjSalI(45),NdeI(61),SmaI(68),EcoRI(71),PstI(81),BamHI(82),XhoI(89),HindIII(95),XbaI(101),ApaI(128),SpeI(181)"Times New Roman-"$uqA+q OsSacI"Times New Roman)$ OsNcoI"Times New Roman$1 OsStuI"Times New Roman$ 0#?/?/{> OsSacII"Times New Roman $HpFC#c OsFspI(3648),AseI(3696)"Times New Roman@$o[[ OsBglII(4837),NheI(4865)"Times New Roman$TS+s+ Ostest"Times New Roman($qyy