Sequence of pDNR-1r

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Sequence of pDNR-1r

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Sequence of pDNR-1r : 4919 BP; 1480 A; 1117 C; 1093 G; 1229 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gcggccgcat aacttcgtat agcatacatt atacgaagtt atcagtcgac ggtaccggac 60

1 cgccggcgta ttgaagcata tcgtatgtaa tatgcttcaa tagtcagctg ccatggcctg 60

.......... .......... loxP .......... .......... ..........

NdeI SmaI,EcoRI PstI,BamHI2) XhoI(89) HindIII(95)

61 atatgcccgg gaattcctgc aggatccgct cgagaagctt tctagaccat tcgtttggcg 120

61 tatacgggcc cttaaggacg tcctaggcga gctcttcgaa agatctggta agcaaaccgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgcgggccca gtaggtaagt gaacatggtc atagctgttt cctaggagat cctggtcatg 180

121 gcgcccgggt catccattca cttgtaccag tatcgacaaa ggatcctcta ggaccagtac 180

.......... .......... ....... M13_reverse_pri ....... ..........

SpeI(181) .......... .......... .......... .......... ..........

181 actagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa 240

181 tgatcacgaa cctaagagtg gttatttttt gcgggccgcc gttggctcgc aagacttgtt 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SacI(297)

241 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga 300

241 taggtctacc tcaagactcc agtaatgacc tagatagttg tcctcaggtt cgctcgagct 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc 360

301 atagtttaat gcggggcggg acggtgagta gcgtcatgac aacattaagt aattcgtaag 360

.......... .......... ........ CAT/CamR ORF 2 ........ ..........

.......... .......... .......... .......... .......... ..........

361 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca 420

361 acggctgtac cttcggtagt gtttgccgta ctacttggac ttagcggtcg ccgtagtcgt 420

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... NcoI(448) .......... .......... ..........

421 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca 480

421 ggaacagcgg aacgcatatt ataaacgggt accacttttg cccccgcttc ttcaacaggt 480

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa 540

481 ataaccggtg caaatttagt tttgaccact ttgagtgggt ccctaaccga ctctgctttt 540

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat 600

541 tgtataagag ttatttggga aatcccttta tccggtccaa aagtggcatt gtgcggtgta 600

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg 660

601 gaacgcttat atacacatct ttgacggcct ttagcagcac cataagtgag gtctcgctac 660

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca 720

661 ttttgcaaag tcaaacgagt accttttgcc acattgttcc cacttgtgat agggtatagt 720

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa 780

721 ggtcgagtgg cagaaagtaa cggtatgctt taaggcctac tcgtaagtag tccgcccgtt 780

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg 840

781 cttacactta tttccggcct attttgaaca cgaataaaaa gaaatgccag aaatttttcc 840

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct 900

841 ggcattatag gtcgacttgc cagaccaata tccatgtaac tcgttgactg actttacgga 900

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt 960

901 gttttacaag aaatgctacg gtaaccctat atagttgcca ccatataggt cactaaaaaa 960

.......... ........ CAT/CamR ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 tctccatttt agcttcctta gctcctgaaa gatccataac ttcgtatagc atacattata 1020

961 agaggtaaaa tcgaaggaat cgaggacttt ctaggtattg aagcatatcg tatgtaatat 1020

.......... .......... ORF 2 .......... loxP ..........

.......... .......... .......... .......... .......... ..........

1021 cgaagttatg cggccgcgac gtccacatat acctgccgtt cactattatt tagtgaaatg 1080

1021 gcttcaatac gccggcgctg caggtgtata tggacggcaa gtgataataa atcactttac 1080

loxP .......... .......... sacB_neg .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agatattatg atattttctg aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga 1140

1081 tctataatac tataaaagac ttaacactaa tttttccgtt gaaatacggg tacgttgtct 1140

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aactataaaa aatacagaga atgaaaagaa acagatagat tttttagttc tttaggcccg 1200

1141 ttgatatttt ttatgtctct tacttttctt tgtctatcta aaaaatcaag aaatccgggc 1200

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tagtctgcaa atccttttat gattttctat caaacaaaag aggaaaatag accagttgca 1260

1201 atcagacgtt taggaaaata ctaaaagata gtttgttttc tccttttatc tggtcaacgt 1260

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atccaaacga gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga 1320

1261 taggtttgct ctcagattat cttactccag cttttcattt agcgcgccca aacaatgact 1320

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt 1380

1321 atttcgtccg ttctggattt tacacatttc ccgtttcaca tatgaaaccg cagtggggaa 1380

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acatatttta ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc 1440

1381 tgtataaaat ccagaaaaaa ataacacgca ttgattgaac ggtagaagtt tgtcctcccg 1440

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa 1500

1441 accttcttcg tctggcgatt gtgtcatgta ttttttcctc tgtacttgct acttgtagtt 1500

.......... .......... sacB_neg .......... .......... ORF 2

.......... .......... .......... .......... .......... ..........

1501 aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc 1560

1501 tttcaaacgt tttgttcgtt gtcataattg gaaatgatgg cgtgacgacc gtcctccgcg 1560

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aactcaagcg tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc 1620

1561 ttgagttcgc aaacgctttc tttgcttggt tttcggtata ttcctttgta tgccgtaaag 1620

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccatattaca cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca 1680

1621 ggtataatgt gcggtactat acgacgttta gggacttgtc gtttttttac tttttatagt 1680

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... StuI(1731)

1681 agttcctgag ttcgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt 1740

1681 tcaaggactc aagctaagca ggtgttaatt tttatagaga agacgttttc cggacctgca 1740

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttgggacagc tggccattac aaaacgctga cggcactgtc gcaaactatc acggctacca 1800

1741 aaccctgtcg accggtaatg ttttgcgact gccgtgacag cgtttgatag tgccgatggt 1800

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt 1860

1801 gtagcagaaa cgtaatcggc ctctaggatt tttacgccta ctgtgtagct aaatgtacaa 1860

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa 1920

1861 gatagttttt cagccgcttt gaagataact gtcgaccttt ttgcgaccgg cgcagaaatt 1920

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agacagcgac aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc 1980

1921 tctgtcgctg tttaagctac gtttactaag ataggatttt ctggtttgtg ttcttaccag 1980

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aggttcagcc acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg 2040

1981 tccaagtcgg tgtaaatgta gactgccttt ttaggcaaat aagatgtgac taaagaggcc 2040

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 taaacattac ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag 2100

2041 atttgtaatg ccgtttgttt gtgactgttg acgtgttcaa ttgcatagtc gtagtctgtc 2100

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctctttgaac atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac 2160

2101 gagaaacttg tagttgccac atctcctaat atttagttag aaactgccac tgcctttttg 2160

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtatcaaaat gtacagcagt tcatcgatga aggcaactac agctcaggcg acaaccatac 2220

2161 catagtttta catgtcgtca agtagctact tccgttgatg tcgagtccgc tgttggtatg 2220

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gctgagagat cctcactacg tagaagataa aggccacaaa tacttagtat ttgaagcaaa 2280

2221 cgactctcta ggagtgatgc atcttctatt tccggtgttt atgaatcata aacttcgttt 2280

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cactggaact gaagatggct accaaggcga agaatcttta tttaacaaag catactatgg 2340

2281 gtgaccttga cttctaccga tggttccgct tcttagaaat aaattgtttc gtatgatacc 2340

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg 2400

2341 gttttcgtgt agtaagaagg cagttctttc agtttttgaa gacgtttcgc tattttttgc 2400

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cacggctgag ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg attacacact 2460

2401 gtgccgactc aatcgtttgc cgcgagagcc atactaactc gatttgctac taatgtgtga 2460

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gaaaaaagtg atgaaaccgc tgattgcatc taacacagta acagatgaaa ttgaacgcgc 2520

2461 cttttttcac tactttggcg actaacgtag attgtgtcat tgtctacttt aacttgcgcg 2520

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... ......... SacII(2569) .........

2521 gaacgtcttt aaaatgaacg gcaaatggta cctgttcact gactcccgcg gatcaaaaat 2580

2521 cttgcagaaa ttttacttgc cgtttaccat ggacaagtga ctgagggcgc ctagttttta 2580

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gacgattgac ggcattacgt ctaacgatat ttacatgctt ggttatgttt ctaattcttt 2640

2581 ctgctaactg ccgtaatgca gattgctata aatgtacgaa ccaatacaaa gattaagaaa 2640

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aactggccca tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc 2700

2641 ttgaccgggt atgttcggcg acttgttttg accggaacac aatttttacc tagaactagg 2700

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 taacgatgta acctttactt actcacactt cgctgtacct caagcgaaag gaaacaatgt 2760

2701 attgctacat tggaaatgaa tgagtgtgaa gcgacatgga gttcgctttc ctttgttaca 2760

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgtgattaca agctatatga caaacagagg attctacgca gacaaacaat caacgtttgc 2820

2761 gcactaatgt tcgatatact gtttgtctcc taagatgcgt ctgtttgtta gttgcaaacg 2820

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcctagcttc ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct 2880

2821 cggatcgaag gacgacttgt agtttccgtt cttttgtaga caacagtttc tgtcgtagga 2880

.......... ........ sacB_neg ORF 2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgaacaagga caattaacag ttaacaaata aaaacgcaaa agaaaatgcc gatatcctat 2940

2881 acttgttcct gttaattgtc aattgtttat ttttgcgttt tcttttacgg ctataggata 2940

.......... ORF 2 sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tggcattgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 3000

2941 accgtaactg cagtccaccg tgaaaagccc ctttacacgc gccttgggga taaacaaata 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 3060

3001 aaaagattta tgtaagttta tacataggcg agtactctgt tattgggact atttacgaag 3060

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 3120

3061 ttattataac tttttccttc tcatactcat aagttgtaaa ggcacagcgg gaataaggga 3120

.......... .......... .......... ....... ORF 3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

3121 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 3180

3121 aaaaacgccg taaaacggaa ggacaaaaac gagtgggtct ttgcgaccac tttcattttc 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 3240

3181 tacgacttct agtcaaccca cgtgctcacc caatgtagct tgacctagag ttgtcgccat 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 3300

3241 tctaggaact ctcaaaagcg gggcttcttg caaaaggtta ctactcgtga aaatttcaag 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 3360

3301 acgatacacc gcgccataat agggcataac tgcggcccgt tctcgttgag ccagcggcgt 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 3420

3361 atgtgataag agtcttactg aaccaactca tgagtggtca gtgtcttttc gtagaatgcc 3420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 3480

3421 taccgtactg tcattctctt aatacgtcac gacggtattg gtactcacta ttgtgacgcc 3480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 3540

3481 ggttgaatga agactgttgc tagcctcctg gcttcctcga ttggcgaaaa aacgtgttgt 3540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 3600

3541 accccctagt acattgagcg gaactagcaa cccttggcct cgacttactt cggtatggtt 3600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 3660

3601 tgctgctcgc actgtggtgc tacggacatc gttaccgttg ttgcaacgcg tttgataatt 3660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... AseI(3696) .......... ..........

3661 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 3720

3661 gaccgcttga tgaatgagat cgaagggccg ttgttaatta tctgacctac ctccgcctat 3720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 3780

3721 ttcaacgtcc tggtgaagac gcgagccggg aaggccgacc gaccaaataa cgactattta 3780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 3840

3781 gacctcggcc actcgcaccc agagcgccat agtaacgtcg tgaccccggt ctaccattcg 3840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 3900

3841 ggagggcata gcatcaatag atgtgctgcc cctcagtccg ttgataccta cttgctttat 3900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 3960

3901 ctgtctagcg actctatcca cggagtgact aattcgtaac cattgacagt ctggttcaaa 3960

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 4020

3961 tgagtatata tgaaatctaa ctaaattttg aagtaaaaat taaattttcc tagatccact 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 4080

4021 tctaggaaaa actattagag tactggtttt agggaattgc actcaaaagc aaggtgactc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 4140

4081 gcagtctggg gcatcttttc tagtttccta gaagaactct aggaaaaaaa gacgcgcatt 4140

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4141 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 4200

4141 agacgacgaa cgtttgtttt tttggtggcg atggtcgcca ccaaacaaac ggcctagttc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 4260

4201 tcgatggttg agaaaaaggc ttccattgac cgaagtcgtc tcgcgtctat ggtttatgac 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 4320

4261 aagaagatca catcggcatc aatccggtgg tgaagttctt gagacatcgt ggcggatgta 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 4380

4321 tggagcgaga cgattaggac aatggtcacc gacgacggtc accgctattc agcacagaat 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 4440

4381 ggcccaacct gagttctgct atcaatggcc tattccgcgt cgccagcccg acttgccccc 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 4500

4441 caagcacgtg tgtcgggtcg aacctcgctt gctggatgtg gcttgactct atggatgtcg 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 4560

4501 cactcgatac tctttcgcgg tgcgaagggc ttccctcttt ccgcctgtcc ataggccatt 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4620

4561 cgccgtccca gccttgtcct ctcgcgtgct ccctcgaagg tccccctttg cggaccatag 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4680

4621 aaatatcagg acagcccaaa gcggtggaga ctgaactcgc agctaaaaac actacgagca 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4740

4681 gtccccccgc ctcggatacc tttttgcggt cgttgcgccg gaaaaatgcc aaggaccgga 4740

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4800

4741 aaacgaccgg aaaacgagtg tacaagaaag gacgcaatag gggactaaga cacctattgg 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gtattaccgc cttacgcgtg taaaacgacg gccagtagat ctgtaatacg actcactata 4860

4801 cataatggcg gaatgcgcac attttgctgc cggtcatcta gacattatgc tgagtgatat 4860

.......... ...... M13_forward20_pri ...... .......... T7_pro

NheI(4865) .......... .......... .......... .......... ..........

4861 gggcgctagc tgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaa. 4919

4861 cccgcgatcg acgagcggcg tcggcttgct ggctcgcgtc gctcagtcac tcgctcctt. 4919

.......... .......... .......... .......... .......... ..........