Relevant mutations/deletions: Altered MCS with AscI and PacI
Vector backbone: pDNR Dual
Backbone manufacturer: Clontech
Type of vector: Cre/Lox,Donor vector
Backbone size (bp): 4827
5' Sequencing primer: M13 forward
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Tony Pawson
Comments: Donor vector for the Creator Splice system.
Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)