Sequence of pDNR

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Sequence of pDNR_Dual

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Sequence of V7 pDNR Dual - Donor : 4827 BP; 1457 A; 1092 C; 1062 G; 1216 T; 0 other;

.......... .......... .......... .......... SalI(43) ..........

1 ataacttcgt atagcataca ttatacgaag ttatggcgcg ccgtcgacgg taccggacat 60

1 tattgaagca tatcgtatgt aatatgcttc aataccgcgc ggcagctgcc atggcctgta 60

.......... loxP .......... .......... .......... ..........

SmaI,EcoRI PstI,BamHI XhoI7) HindIII(93) XbaI(99)

61 atgcccggga attcctgcag gatccgctcg agaagctttc tagaccattc gtttggcgcg 120

61 tacgggccct taaggacgtc ctaggcgagc tcttcgaaag atctggtaag caaaccgcgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... SpeI(166) ..........

121 cgggccttaa ttaaccaggt aagttcctag gagatcctgg tcatgactag tgcttggatt 180

121 gcccggaatt aattggtcca ttcaaggatc ctctaggacc agtactgatc acgaacctaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc 240

181 gagtggttat tttttgcggg ccgccgttgg ctcgcaagac ttgtttaggt ctacctcaag 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... SacI(282) ..........

241 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc 300

241 actccagtaa tgacctagat agttgtcctc aggttcgctc gagctatagt ttaatgcggg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc 360

301 gcgggacggt gagtagcgtc atgacaacat taagtaattc gtaagacggc tgtaccttcg 360

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg 420

361 gtagtgtttg ccgtactact tggacttagc ggtcgccgta gtcgtggaac agcggaacgc 420

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... NcoI(433) .......... .......... .......... ..........

421 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta 480

421 atattataaa cgggtaccac ttttgccccc gcttcttcaa caggtataac cggtgcaaat 480

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa 540

481 ttagttttga ccactttgag tgggtcccta accgactctg ctttttgtat aagagttatt 540

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

541 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt 600

541 tgggaaatcc ctttatccgg tccaaaagtg gcattgtgcg gtgtagaacg cttatataca 600

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt 660

601 catctttgac ggcctttagc agcaccataa gtgaggtctc gctacttttg caaagtcaaa 660

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt 720

661 cgagtacctt ttgccacatt gttcccactt gtgatagggt atagtggtcg agtggcagaa 720

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg 780

721 agtaacggta tgctttaagg cctactcgta agtagtccgc ccgttcttac acttatttcc 780

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct 840

781 ggcctatttt gaacacgaat aaaaagaaat gccagaaatt tttccggcat tataggtcga 840

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac 900

841 cttgccagac caatatccat gtaactcgtt gactgacttt acggagtttt acaagaaatg 900

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt 960

901 ctacggtaac cctatatagt tgccaccata taggtcacta aaaaaagagg taaaatcgaa 960

.......... ..... CAT/CamR Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .... EagI(1016),NotI(1016)

961 ccttagctcc tgaaagatcc ataacttcgt atagcataca ttatacgaag ttatgcggcc 1020

961 ggaatcgagg actttctagg tattgaagca tatcgtatgt aatatgcttc aatacgccgg 1020

......... Orf frame 2 ......... loxP .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcgacgtcca catatacctg ccgttcacta ttatttagtg aaatgagata ttatgatatt 1080

1021 cgctgcaggt gtatatggac ggcaagtgat aataaatcac tttactctat aatactataa 1080

.......... .......... .......... sacB_neg .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttctgaattg tgattaaaaa ggcaacttta tgcccatgca acagaaacta taaaaaatac 1140

1081 aagacttaac actaattttt ccgttgaaat acgggtacgt tgtctttgat attttttatg 1140

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agagaatgaa aagaaacaga tagatttttt agttctttag gcccgtagtc tgcaaatcct 1200

1141 tctcttactt ttctttgtct atctaaaaaa tcaagaaatc cgggcatcag acgtttagga 1200

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tttatgattt tctatcaaac aaaagaggaa aatagaccag ttgcaatcca aacgagagtc 1260

1201 aaatactaaa agatagtttg ttttctcctt ttatctggtc aacgttaggt ttgctctcag 1260

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 taatagaatg aggtcgaaaa gtaaatcgcg cgggtttgtt actgataaag caggcaagac 1320

1261 attatcttac tccagctttt catttagcgc gcccaaacaa tgactatttc gtccgttctg 1320

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctaaaatgtg taaagggcaa agtgtatact ttggcgtcac cccttacata ttttaggtct 1380

1321 gattttacac atttcccgtt tcacatatga aaccgcagtg gggaatgtat aaaatccaga 1380

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttttttattg tgcgtaacta acttgccatc ttcaaacagg agggctggaa gaagcagacc 1440

1381 aaaaaataac acgcattgat tgaacggtag aagtttgtcc tcccgacctt cttcgtctgg 1440

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctaacacag tacataaaaa aggagacatg aacgatgaac atcaaaaagt t tgcaaaaca 1500

1441 cgattgtgtc atgtattttt tcctctgtac ttgctacttg tagtttttca aacgttttgt 1500

.......... .......... sacB_neg ......... Orf frame 2 .........

.......... .......... .......... .......... .......... ..........

1501 agcaacagta ttaaccttta ctaccgcact gctggcagga ggcgcaactc aagcgtttgc 1560

1501 tcgttgtcat aattggaaat gatggcgtga cgaccgtcct ccgcgttgag ttcgcaaacg 1560

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gaaagaaacg aaccaaaagc catataagga aacatacggc atttcccata ttacacgcca 1620

1561 ctttctttgc ttggttttcg gtatattcct ttgtatgccg taaagggtat aatgtgcggt 1620

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgatatgctg caaatccctg aacagcaaaa aaatgaaaaa tatcaagttc ctgagttcga 1680

1621 actatacgac gtttagggac ttgtcgtttt tttacttttt atagttcaag gactcaagct 1680

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... StuI(1716) .......... ..........

1681 ttcgtccaca attaaaaata tctcttctgc aaaaggcctg gacgtttggg acagctggcc 1740

1681 aagcaggtgt taatttttat agagaagacg ttttccggac ctgcaaaccc tgtcgaccgg 1740

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 attacaaaac gctgacggca ctgtcgcaaa ctatcacggc taccacatcg tctttgcatt 1800

1741 taatgttttg cgactgccgt gacagcgttt gatagtgccg atggtgtagc agaaacgtaa 1800

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agccggagat cctaaaaatg cggatgacac atcgatttac atgttctatc aaaaagtcgg 1860

1801 tcggcctcta ggatttttac gcctactgtg tagctaaatg tacaagatag tttttcagcc 1860

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgaaacttct attgacagct ggaaaaacgc tggccgcgtc tttaaagaca gcgacaaatt 1920

1861 gctttgaaga taactgtcga cctttttgcg accggcgcag aaatttctgt cgctgtttaa 1920

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cgatgcaaat gattctatcc taaaagacca aacacaagaa tggtcaggtt cagccacatt 1980

1921 gctacgttta ctaagatagg attttctggt ttgtgttctt accagtccaa gtcggtgtaa 1980

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tacatctgac ggaaaaatcc gtttattcta cactgatttc tccggtaaac attacggcaa 2040

1981 atgtagactg cctttttagg caaataagat gtgactaaag aggccatttg taatgccgtt 2040

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 acaaacactg acaactgcac aagttaacgt atcagcatca gacagctctt tgaacatcaa 2100

2041 tgtttgtgac tgttgacgtg ttcaattgca tagtcgtagt ctgtcgagaa acttgtagtt 2100

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cggtgtagag gattataaat caatctttga cggtgacgga aaaacgtatc aaaatgtaca 2160

2101 gccacatctc ctaatattta gttagaaact gccactgcct ttttgcatag ttttacatgt 2160

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcagttcatc gatgaaggca actacagctc aggcgacaac catacgctga gagatcctca 2220

2161 cgtcaagtag ctacttccgt tgatgtcgag tccgctgttg gtatgcgact ctctaggagt 2220

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctacgtagaa gataaaggcc acaaatactt agtatttgaa gcaaacactg gaactgaaga 2280

2221 gatgcatctt ctatttccgg tgtttatgaa tcataaactt cgtttgtgac cttgacttct 2280

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tggctaccaa ggcgaagaat ctttatttaa caaagcatac tatggcaaaa gcacatcatt 2340

2281 accgatggtt ccgcttctta gaaataaatt gtttcgtatg ataccgtttt cgtgtagtaa 2340

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cttccgtcaa gaaagtcaaa aacttctgca aagcgataaa aaacgcacgg ctgagttagc 2400

2341 gaaggcagtt ctttcagttt ttgaagacgt ttcgctattt tttgcgtgcc gactcaatcg 2400

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aaacggcgct ctcggtatga ttgagctaaa cgatgattac acactgaaaa aagtgatgaa 2460

2401 tttgccgcga gagccatact aactcgattt gctactaatg tgtgactttt ttcactactt 2460

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 accgctgatt gcatctaaca cagtaacaga tgaaattgaa cgcgcgaacg tctttaaaat 2520

2461 tggcgactaa cgtagattgt gtcattgtct actttaactt gcgcgcttgc agaaatttta 2520

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... ......... SacII(2554) ......... ..........

2521 gaacggcaaa tggtacctgt tcactgactc ccgcggatca aaaatgacga ttgacggcat 2580

2521 cttgccgttt accatggaca agtgactgag ggcgcctagt ttttactgct aactgccgta 2580

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tacgtctaac gatatttaca tgcttggtta tgtttctaat tctttaactg gcccatacaa 2640

2581 atgcagattg ctataaatgt acgaaccaat acaaagatta agaaattgac cgggtatgtt 2640

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gccgctgaac aaaactggcc ttgtgttaaa aatggatctt gatcctaacg atgtaacctt 2700

2641 cggcgacttg ttttgaccgg aacacaattt ttacctagaa ctaggattgc tacattggaa 2700

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tacttactca cacttcgctg tacctcaagc gaaaggaaac aatgtcgtga ttacaagcta 2760

2701 atgaatgagt gtgaagcgac atggagttcg ctttcctttg ttacagcact aatgttcgat 2760

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tatgacaaac agaggattct acgcagacaa acaatcaacg tttgcgccta gcttcctgct 2820

2761 atactgtttg tctcctaaga tgcgtctgtt tgttagttgc aaacgcggat cgaaggacga 2820

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gaacatcaaa ggcaagaaaa catctgttgt caaagacagc atccttgaac aaggacaatt 2880

2821 cttgtagttt ccgttctttt gtagacaaca gtttctgtcg taggaacttg ttcctgttaa 2880

.......... ..... sacB_neg Orf frame 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aacagttaac aaataaaaac gcaaaagaaa atgccgatat cctattggca ttgacgtcag 2940

2881 ttgtcaattg tttatttttg cgttttcttt tacggctata ggataaccgt aactgcagtc 2940

Orf frame 2 ........ sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 3000

2941 caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 3060

3001 gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt 3060

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 3120

3061 ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa 3120

.......... .......... .... Orf frame 3 Ampicillin .... ..........

.......... .......... .......... .......... .......... ..........

3121 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 3180

3121 cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca 3180

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 3240

3181 acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa 3240

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 3300

3241 aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc 3300

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 3360

3301 ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct 3360

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 3420

3361 tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt 3420

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 3480

3421 ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact 3480

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 3540

3481 gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt 3540

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 3600

3541 gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt 3600

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... FspI(3633) .......... ..........

3601 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 3660

3601 ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat 3660

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... AseI(3681) .......... .......... ..........

3661 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 3720

3661 gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg 3720

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 3780

3721 aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg 3780

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 3840

3781 cacccagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc 3840

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 3900

3841 aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct 3900

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 3960

3901 atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa 3960

.... Orf frame 3 Ampicillin .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 4020

3961 tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 4080

4021 tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 4140

4081 ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt 4140

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4141 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 4200

4141 gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 4260

4201 aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa gatcacatcg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 4320

4261 gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 4380

4321 aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 4440

4381 ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 4500

4441 ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 4560

4501 cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 4620

4561 gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 4680

4621 ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 4740

4681 ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac 4740

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccttac 4800

4741 gagtgtacaa gaaaggacgc aataggggac taagacacct attggcataa tggcggaatg 4800

.......... .......... .......... .......... .......... ..........

.......... ......... BglII(4822) ......... .......... ..........

4801 gcgtgtaaaa cgacggccag tagatct... .......... .......... .......... 4827

4801 cgcacatttt gctgccggtc atctaga... .......... .......... .......... 4827

..... M13_forward20_primer ..... .......... .......... ..........