Sequence of pDNR

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Sequence of pDNR

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Sequence of V308 pDNR MCS lacZ alpha: 5138 BP; 1535 A; 1183 C; 1134 G; 1286 T; 0 other;

.......... .......... .......... .......... NarI(44) ..........

1 ataacttcgt atagcataca ttatacgaag ttatggcgcg ccggcgccca atacgcaaac 60

1 tattgaagca tatcgtatgt aatatgcttc aataccgcgc ggccgcgggt tatgcgtttg 60

.......... loxP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 120

61 gcggagaggg gcgcgcaacc ggctaagtaa ttacgtcgac cgtgctgtcc aaagggctga 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 180

121 cctttcgccc gtcactcgcg ttgcgttaat tacactcaat cgagtgagta atccgtgggg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aggctttaca ctttatgctt ccggctcgta tgttgtgtga aattgtgagc ggataacaat 240

181 tccgaaatgt gaaatacgaa ggccgagcat acaacacact ttaacactcg cctattgtta 240

......... lac_promoter ......... .......... ...... M13_pUC_rev_primer

.......... .......... .......... .......... .......... ..........

241 ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt tttacaacgt 300

241 aagtgtgtcc tttgtcgata ctggtactaa tgcctaagtg accggcagca aaatgttgca 300

M13_pUC_rev_primer M13_reverse_primer lacZ_a M13_forward20_primer M13_pUC_fwd_primer

.......... .......... .......... .......... .......... ..........

301 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 360

301 gcactgaccc ttttgggacc gcaatgggtt gaattagcgg aacgtcgtgt agggggaaag 360

M13_pUC_fwd_primer .. lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 420

361 cggtcgaccg cattatcgct tctccgggcg tggctagcgg gaagggttgt caacgcgtcg 420

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... SpeI(477)

421 ctgaatggcg aatggcgtta attaaccagg taagttccta ggagatcctg gtcatgacta 480

421 gacttaccgc ttaccgcaat taattggtcc attcaaggat cctctaggac cagtactgat 480

lacZ_a .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc 540

481 cacgaaccta agagtggtta ttttttgcgg gccgccgttg gctcgcaaga cttgtttagg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SacI(593)

541 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc 600

541 tctacctcaa gactccagta atgacctaga tagttgtcct caggttcgct cgagctatag 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc 660

601 tttaatgcgg ggcgggacgg tgagtagcgt catgacaaca ttaagtaatt cgtaagacgg 660

.......... .......... ..... CAT/CamR Orf frame 1 ..... ..........

.......... .......... .......... .......... .......... ..........

661 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt 720

661 ctgtaccttc ggtagtgttt gccgtactac ttggacttag cggtcgccgt agtcgtggaa 720

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... NcoI(744) .......... .......... ..........

721 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt 780

721 cagcggaacg catattataa acgggtacca cttttgcccc cgcttcttca acaggtataa 780

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat 840

781 ccggtgcaaa tttagttttg accactttga gtgggtccct aaccgactct gctttttgta 840

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

841 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg 900

841 taagagttat ttgggaaatc cctttatccg gtccaaaagt ggcattgtgc ggtgtagaac 900

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa 960

901 gcttatatac acatctttga cggcctttag cagcaccata agtgaggtct cgctactttt 960

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag 1020

961 gcaaagtcaa acgagtacct tttgccacat tgttcccact tgtgataggg tatagtggtc 1020

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat 1080

1021 gagtggcaga aagtaacggt atgctttaag gcctactcgt aagtagtccg cccgttctta 1080

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt 1140

1081 cacttatttc cggcctattt tgaacacgaa taaaaagaaa tgccagaaat ttttccggca 1140

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa 1200

1141 ttataggtcg acttgccaga ccaatatcca tgtaactcgt tgactgactt tacggagttt 1200

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc 1260

1201 tacaagaaat gctacggtaa ccctatatag ttgccaccat ataggtcact aaaaaaagag 1260

.......... ..... CAT/CamR Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cattttagct tccttagctc ctgaaagatc cataacttcg tatagcatac attatacgaa 1320

1261 gtaaaatcga aggaatcgag gactttctag gtattgaagc atatcgtatg taatatgctt 1320

.......... ......... Orf frame 1 ......... loxP ..........

EagI(1327),NotI(1327) ........ .......... .......... ..........

1321 gttatgcggc cgcgacgtcc acatatacct gccgttcact attatttagt gaaatgagat 1380

1321 caatacgccg gcgctgcagg tgtatatgga cggcaagtga taataaatca ctttactcta 1380

.......... .......... .......... sacB_neg .......... ..........

.......... .......... .......... .......... .......... ..........

1381 attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc aacagaaact 1440

1381 taatactata aaagacttaa cactaatttt tccgttgaaa tacgggtacg ttgtctttga 1440

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta ggcccgtagt 1500

1441 tattttttat gtctcttact tttctttgtc tatctaaaaa atcaagaaat ccgggcatca 1500

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca gttgcaatcc 1560

1501 gacgtttagg aaaatactaa aagatagttt gttttctcct tttatctggt caacgttagg 1560

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt tactgataaa 1620

1561 tttgctctca gattatctta ctccagcttt tcatttagcg cgcccaaaca atgactattt 1620

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca ccccttacat 1680

1621 cgtccgttct ggattttaca catttcccgt ttcacatatg aaaccgcagt ggggaatgta 1680

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag gagggctgga 1740

1681 taaaatccag aaaaaaataa cacgcattga ttgaacggta gaagtttgtc ctcccgacct 1740

.......... .......... sacB_neg .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa catcaaaaag 1800

1741 tcttcgtctg gcgattgtgt catgtatttt ttcctctgta cttgctactt gtagtttttc 1800

.......... .......... sacB_neg .......... ......... Orf frame 1

.......... .......... .......... .......... .......... ..........

1801 tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg aggcgcaact 1860

1801 aaacgttttg ttcgttgtca taattggaaa tgatggcgtg acgaccgtcc tccgcgttga 1860

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg catttcccat 1920

1861 gttcgcaaac gctttctttg cttggttttc ggtatattcc tttgtatgcc gtaaagggta 1920

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa atatcaagtt 1980

1921 taatgtgcgg tactatacga cgtttaggga cttgtcgttt ttttactttt tatagttcaa 1980

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... StuI(2027) ..........

1981 cctgagttcg attcgtccac aattaaaaat atctcttctg caaaaggcct ggacgtttgg 2040

1981 ggactcaagc taagcaggtg ttaattttta tagagaagac gttttccgga cctgcaaacc 2040

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg ctaccacatc 2100

2041 ctgtcgaccg gtaatgtttt gcgactgccg tgacagcgtt tgatagtgcc gatggtgtag 2100

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta catgttctat 2160

2101 cagaaacgta atcggcctct aggattttta cgcctactgt gtagctaaat gtacaagata 2160

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt ctttaaagac 2220

2161 gtttttcagc cgctttgaag ataactgtcg acctttttgc gaccggcgca gaaatttctg 2220

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga atggtcaggt 2280

2221 tcgctgttta agctacgttt actaagatag gattttctgg tttgtgttct taccagtcca 2280

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt ctccggtaaa 2340

2281 agtcggtgta aatgtagact gcctttttag gcaaataaga tgtgactaaa gaggccattt 2340

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc agacagctct 2400

2341 gtaatgccgt ttgtttgtga ctgttgacgt gttcaattgc atagtcgtag tctgtcgaga 2400

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg aaaaacgtat 2460

2401 aacttgtagt tgccacatct cctaatattt agttagaaac tgccactgcc tttttgcata 2460

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa ccatacgctg 2520

2461 gttttacatg tcgtcaagta gctacttccg ttgatgtcga gtccgctgtt ggtatgcgac 2520

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga agcaaacact 2580

2521 tctctaggag tgatgcatct tctatttccg gtgtttatga atcataaact tcgtttgtga 2580

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata ctatggcaaa 2640

2581 ccttgacttc taccgatggt tccgcttctt agaaataaat tgtttcgtat gataccgttt 2640

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa aaaacgcacg 2700

2641 tcgtgtagta agaaggcagt tctttcagtt tttgaagacg tttcgctatt ttttgcgtgc 2700

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta cacactgaaa 2760

2701 cgactcaatc gtttgccgcg agagccatac taactcgatt tgctactaat gtgtgacttt 2760

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga acgcgcgaac 2820

2761 tttcactact ttggcgacta acgtagattg tgtcattgtc tactttaact tgcgcgcttg 2820

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... KpnI(2847) ......... SacII(2865) .........

2821 gtctttaaaa tgaacggcaa atggtacctg ttcactgact cccgcggatc aaaaatgacg 2880

2821 cagaaatttt acttgccgtt taccatggac aagtgactga gggcgcctag tttttactgc 2880

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa ttctttaact 2940

2881 taactgccgt aatgcagatt gctataaatg tacgaaccaa tacaaagatt aagaaattga 2940

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct tgatcctaac 3000

2941 ccgggtatgt tcggcgactt gttttgaccg gaacacaatt tttacctaga actaggattg 3000

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa caatgtcgtg 3060

3001 ctacattgga aatgaatgag tgtgaagcga catggagttc gctttccttt gttacagcac 3060

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac gtttgcgcct 3120

3061 taatgttcga tatactgttt gtctcctaag atgcgtctgt ttgttagttg caaacgcgga 3120

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag catccttgaa 3180

3121 tcgaaggacg acttgtagtt tccgttcttt tgtagacaac agtttctgtc gtaggaactt 3180

.......... ..... sacB_neg Orf frame 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 caaggacaat taacagttaa caaataaaaa cgcaaaagaa aatgccgata tcctattggc 3240

3181 gttcctgtta attgtcaatt gtttattttt gcgttttctt ttacggctat aggataaccg 3240

......... Orf frame 1 sacB_neg ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 attgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 3300

3241 taactgcagt ccaccgtgaa aagccccttt acacgcgcct tggggataaa caaataaaaa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 3360

3301 gatttatgta agtttataca taggcgagta ctctgttatt gggactattt acgaagttat 3360

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 3420

3361 tataactttt tccttctcat actcataagt tgtaaaggca cagcgggaat aagggaaaaa 3420

.......... .......... .... Orf frame 2 Ampicillin .... ..........

.......... .......... .......... .......... .......... ..........

3421 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 3480

3421 acgccgtaaa acggaaggac aaaaacgagt gggtctttgc gaccactttc attttctacg 3480

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 3540

3481 acttctagtc aacccacgtg ctcacccaat gtagcttgac ctagagttgt cgccattcta 3540

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 3600

3541 ggaactctca aaagcggggc ttcttgcaaa aggttactac tcgtgaaaat ttcaagacga 3600

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 3660

3601 tacaccgcgc cataataggg cataactgcg gcccgttctc gttgagccag cggcgtatgt 3660

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 3720

3661 gataagagtc ttactgaacc aactcatgag tggtcagtgt cttttcgtag aatgcctacc 3720

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 3780

3721 gtactgtcat tctcttaata cgtcacgacg gtattggtac tcactattgt gacgccggtt 3780

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 3840

3781 gaatgaagac tgttgctagc ctcctggctt cctcgattgg cgaaaaaacg tgttgtaccc 3840

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 3900

3841 cctagtacat tgagcggaac tagcaaccct tggcctcgac ttacttcggt atggtttgct 3900

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 3960

3901 gctcgcactg tggtgctacg gacatcgtta ccgttgttgc aacgcgtttg ataattgacc 3960

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 4020

3961 gcttgatgaa tgagatcgaa gggccgttgt taattatctg acctacctcc gcctatttca 4020

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 4080

4021 acgtcctggt gaagacgcga gccgggaagg ccgaccgacc aaataacgac tatttagacc 4080

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 4140

4081 tcggccactc gcacccagag cgccatagta acgtcgtgac cccggtctac cattcgggag 4140

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 4200

4141 ggcatagcat caatagatgt gctgcccctc agtccgttga tacctacttg ctttatctgt 4200

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 4260

4201 ctagcgactc tatccacgga gtgactaatt cgtaaccatt gacagtctgg ttcaaatgag 4260

.... Orf frame 2 Ampicillin .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 4320

4261 tatatatgaa atctaactaa attttgaagt aaaaattaaa ttttcctaga tccacttcta 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 4380

4321 ggaaaaacta ttagagtact ggttttaggg aattgcactc aaaagcaagg tgactcgcag 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 4440

4381 tctggggcat cttttctagt ttcctagaag aactctagga aaaaaagacg cgcattagac 4440

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4441 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 4500

4441 gacgaacgtt tgtttttttg gtggcgatgg tcgccaccaa acaaacggcc tagttctcga 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 4560

4501 tggttgagaa aaaggcttcc attgaccgaa gtcgtctcgc gtctatggtt tatgacaaga 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 4620

4561 agatcacatc ggcatcaatc cggtggtgaa gttcttgaga catcgtggcg gatgtatgga 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 4680

4621 gcgagacgat taggacaatg gtcaccgacg acggtcaccg ctattcagca cagaatggcc 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 4740

4681 caacctgagt tctgctatca atggcctatt ccgcgtcgcc agcccgactt gccccccaag 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 4800

4741 cacgtgtgtc gggtcgaacc tcgcttgctg gatgtggctt gactctatgg atgtcgcact 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 4860

4801 cgatactctt tcgcggtgcg aagggcttcc ctctttccgc ctgtccatag gccattcgcc 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 4920

4861 gtcccagcct tgtcctctcg cgtgctccct cgaaggtccc cctttgcgga ccatagaaat 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 4980

4921 atcaggacag cccaaagcgg tggagactga actcgcagct aaaaacacta cgagcagtcc 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 5040

4981 ccccgcctcg gatacctttt tgcggtcgtt gcgccggaaa aatgccaagg accggaaaac 5040

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 5100

5041 gaccggaaaa cgagtgtaca agaaaggacg caatagggga ctaagacacc tattggcata 5100

.......... .......... .......... .......... .......... ..........

.......... .......... ......... BglII(5133) ......... ..........

5101 taccgcctta cgcgtgtaaa acgacggcca gtagatct.. .......... .......... 5138

5101 atggcggaat gcgcacattt tgctgccggt catctaga.. .......... .......... 5138

.......... ..... M13_forward20_primer ..... .......... ..........