Sequence of pDW1775

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Sequence of pDW1775

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Sequence of pDW1775 : 5528 BP; 1582 A; 1151 C; 1276 G; 1519 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtcaacgaca tcacctggtt gcgagccttc tgaaggactc ctgttgatag atccagtaat 60

1 cagttgctgt agtggaccaa cgctcggaag acttcctgag gacaactatc taggtcatta 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat 120

61 ctggagtctt gaggtagacc taaacaagtc ttgcgagcca acggcggccc gcaaaaaata 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tggtgagaat caggcgtaat acgactcact atagggcgaa tggaactgga cgctcgcctg 180

121 accactctta gtccgcatta tgctgagtga tatcccgctt accttgacct gcgagcggac 180

.......... ......... T7_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tggtcgcagg gtggctcgta cgaccgcagg catcaaataa aacgaaaggc tcagtcgaaa 240

181 accagcgtcc caccgagcat gctggcgtcc gtagtttatt ttgctttccg agtcagcttt 240

.......... .......... .......... ...... rrnB_T1_terminator ......

.......... .......... .......... .......... .......... ..........

241 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat 300

241 ctgacccgga aagcaaaata gacaacaaac agccacttgc gagaggactc atcctgttta 300

rrnB_T1_terminator .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgccagcgg atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg 360

301 ggcggtcgcc tactcgcctc ttaattccct cagtgcaata ctgggggcgg ctactgcgcc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg 420

361 ctgttcggca aaatgcaaac cttgactgtc ttggcgttgc aacttcctcg gtgagtcggc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa 480

421 ggccaaagac ctcaaattac tcgattcgtg tatgcagtct ttggtaataa cgcgcaagtt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aagtcgccta aggtcactat caggtagcaa atatttcttg tcaaaaatgc tccactgacg 540

481 ttcagcggat tccagtgata gtccatcgtt tataaagaac agtttttacg aggtgactgc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... BglII(588) XhoI(596)

541 ttccataaat tcccctcggt atccaattag aaaggtggga ctcacgaaga tctaactcga 600

541 aaggtattta aggggagcca taggttaatc tttccaccct gagtgcttct agattgagct 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gaaagatatt gtatatatcg taacaatagg aggttcaaca atggcttcct cccctccaaa 660

601 ctttctataa catatatagc attgttatcc tccaagttgt taccgaagga ggggaggttt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ..... XbaI(701) AgeI(714) BamHI(720)

661 gaaaaagaga aaggttagtt ggaaggacgc aagtggttgg tctagacccg ggaaccggtg 720

661 ctttttctct ttccaatcaa ccttcctgcg ttcaccaacc agatctgggc ccttggccac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gatcccagct agtgaaatct gaattggaag agaagaaatc tgaacttaga cataaattga 780

721 ctagggtcga tcactttaga cttaaccttc tcttctttag acttgaatct gtatttaact 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aatatgtgcc acatgaatat attgaattga ttgaaatcgc aagaaattca actcaggata 840

781 ttatacacgg tgtacttata taacttaact aactttagcg ttctttaagt tgagtcctat 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gaatccttga aatgaaggtg atggagttct ttatgaaggt ttatggttat cgtggtaaac 900

841 cttaggaact ttacttccac tacctcaaga aatacttcca aataccaata gcaccatttg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atttgggtgg atcaaggaaa ccagacggag caatttatac tgtcggatct cctattgatt 960

901 taaacccacc tagttccttt ggtctgcctc gttaaatatg acagcctaga ggataactaa 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 acggtgtgat cgttgatact aaggcatatt caggaggtta taatcttcca attggtcaag 1020

961 tgccacacta gcaactatga ttccgtataa gtcctccaat attagaaggt taaccagttc 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cagatgaaat gcaaagatat gtcgaagaga atcaaacaag aaacaagcat atcaacccta 1080

1021 gtctacttta cgtttctata cagcttctct tagtttgttc tttgttcgta tagttgggat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atgaatggtg gaaagtctat ccatcttcag taacagaatt taagttcttg tttgtgagtg 1140

1081 tacttaccac ctttcagata ggtagaagtc attgtcttaa attcaagaac aaacactcac 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtcatttcaa aggaaactac aaagctcagc ttacaagatt gaatcatatc actaattgta 1200

1141 cagtaaagtt tcctttgatg tttcgagtcg aatgttctaa cttagtatag tgattaacat 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atggagctgt tcttagtgta gaagagcttt tgattggtgg agaaatgatt aaagctggta 1260

1201 tacctcgaca agaatcacat cttctcgaaa actaaccacc tctttactaa tttcgaccat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cattgacact tgaggaagtg agaaggaaat ttaataacgg tgagataaac ttttaatagg 1320

1261 gtaactgtga actccttcac tcttccttta aattattgcc actctatttg aaaattatcc 1320

.......... .......... .......... .......... .......... ..........

SacI(1324) .......... .......... ......... AflII(1365) .........

1321 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct 1380

1321 tcgagcttaa aggggctagc aagtttgtaa accgttattt caaagaattc taacttagga 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata 1440

1381 caacggccag aacgctacta atagtatatt aaagacaact taatgcaatt cgtacattat 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa 1500

1441 taattgtaca ttacgtactg caataaatac tctacccaaa aatactaatc tcagggcgtt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg 1560

1501 aatatgtaaa ttatgcgcta tcttttgttt tatatcgcgc gtttgatcct atttaatagc 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ApaI(1595) .......... ..........

1561 cgcgcggtgt catctatgtt actagatcgt gggcccacat tcctgtagaa ctcggtgaga 1620

1561 gcgcgccaca gtagatacaa tgatctagca cccgggtgta aggacatctt gagccactct 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cttttgttcc ctttagtgag ggttaattgt ccaaaaaaaa aggctccaaa aggagccttt 1680

1621 gaaaacaagg gaaatcactc ccaattaaca ggtttttttt tccgaggttt tcctcggaaa 1680

......... T3_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aattgtttcg gtttatgctt aggacaccat tgattaacgc ttacaatttc ctgatgcggt 1740

1681 ttaacaaagc caaatacgaa tcctgtggta actaattgcg aatgttaaag gactacgcca 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 attttctcct tacgcatctg tgcggtattt cacaccgcat agatccgtcg agttcaagag 1800

1741 taaaagagga atgcgtagac acgccataaa gtgtggcgta tctaggcagc tcaagttctc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aaaaaaaaag aaaaagcaaa aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta 1860

1801 tttttttttc tttttcgttt ttcttttttc ctttcgcgcg gagcaagtct tactgtgcat 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tagaatgatg cattaccttg tcatcttcag tatcatactg ttcgtataaa tacttactga 1920

1861 atcttactac gtaatggaac agtagaagtc atagtatgac aagcatattt atgaatgact 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cattcatagc tatacatata tacacatgta tatatatcgt atgctggagc tttaaataat 1980

1921 gtaagtatcg atatgtatat atgtgtacat atatatagca tacgacctcg aaatttatta 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cggtgtcact acataagaac acctttggtg gagggaacat cgttggttcc attgggcgag 2040

1981 gccacagtga tgtattcttg tggaaaccac ctcccttgta gcaaccaagg taacccgctc 2040

.......... .......... .. Orf frame 3 HIS3(variant) .. ..........

.......... .......... .......... .......... .......... ..........

2041 gtggcttctc ttatggcaac cgcaagagcc ttgaacgcac tctcactacg gtgatggtca 2100

2041 caccgaagag aataccgttg gcgttctcgg aacttgcgtg agagtgatgc cactaccagt 2100

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttcttgcctc gcagacaatc aacgtggagg gtaattctgc ttgcctctgc aaaactttca 2160

2101 aagaacggag cgtctgttag ttgcacctcc cattaagacg aacggagacg ttttgaaagt 2160

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agaaaatgcg ggatcatctc gcaagagagg tctcctactt tctccctttg caaaccaagt 2220

2161 tcttttacgc cctagtagag cgttctctcc agaggatgaa agagggaaac gtttggttca 2220

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcgacaactg cgtatggcct gttagaaagg tctaccaccg ctctggaaag tgcctcatcc 2280

2221 agctgttgac gcataccgga caatctttcc agatggtggc gagacctttc acggagtagg 2280

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaaggcgcaa atcctgatcc aaaccttttt actccacgcg ccagtagagc ctctttaaaa 2340

2281 tttccgcgtt taggactagg tttggaaaaa tgaggtgcgc ggtcatctcg gagaaatttt 2340

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcctgaccga gagcaatccc gcagtcttca gtggtgtgat ggtcgtctat gtgtaagtca 2400

2341 cggactggct ctcgttaggg cgtcagaagt caccacacta ccagcagata cacattcagt 2400

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... MscI(2440) .......... ..........

2401 ccaatgcact caacgattag cgaccagccg gaatgcttgg ccagagcatg tatcatgtgg 2460

2401 ggttacgtga gttgctaatc gctggtcggc cttacgaacc ggtctcgtac atagtacacc 2460

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tccagaaacc ctatacctgt gtggacgtta atcacttgcg attgtgtggc ctgttctgct 2520

2461 aggtctttgg gatatggaca cacctgcaat tagtgaacgc taacacaccg gacaagacga 2520

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2521 actgcttctg cctctttttc tgggaagatc gagtgctcta tcgctagggg accacccttt 2580

2521 tgacgaagac ggagaaaaag acccttctag ctcacgagat agcgatcccc tggtgggaaa 2580

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aaagagatag caatctgaat cttggtttca tttgtaatac gctttactag ggctttctgc 2640

2581 tttctctatc gttagactta gaaccaaagt aaacattatg cgaaatgatc ccgaaagacg 2640

.......... .. Orf frame 3 HIS3(variant) .. .......... ..........

.......... .......... .......... .......... ......... BstBI(2693)

2641 tctgtcatct ttgccttcgt ttatcttgcc tgctcatttt ttagtatatt cttcgaagaa 2700

2641 agacagtaga aacggaagca aatagaacgg acgagtaaaa aatcatataa gaagcttctt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atcacattac tttatataat gtataattca ttatgtgata atgccaatcg ctaagaaaaa 2760

2701 tagtgtaatg aaatatatta catattaagt aatacactat tacggttagc gattcttttt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaaagagtca tccgctaggt ggaaaaaaaa aaatgaaaat cattaccgag gcataaaaaa 2820

2761 ttttctcagt aggcgatcca cctttttttt tttactttta gtaatggctc cgtatttttt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atatagagtg tactagagga ggccaagagt aatagaaaaa gaaaattgcg ggaaaggact 2880

2821 tatatctcac atgatctcct ccggttctca ttatcttttt cttttaacgc cctttcctga 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtgttatgac ttccctgact aatgccgtgt tcaaacgata cctggcagtg actcctagcg 2940

2881 cacaatactg aagggactga ttacggcaca agtttgctat ggaccgtcac tgaggatcgc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctcaccaagc tcttaaaacg ggaatttatg gatcttaatt aagacggatc gcttgcctgt 3000

2941 gagtggttcg agaattttgc ccttaaatac ctagaattaa ttctgcctag cgaacggaca 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aacttacacg cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt 3060

3001 ttgaatgtgc gcggagcata gaaaattact accttattaa acccttaaat gagacacaaa 3060

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atttattttt atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta 3120

3061 taaataaaaa tacaaaacat aaacctaaaa tctttcattt atttcttcca tcttctcaat 3120

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cggaatgaag aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta 3180

3121 gccttacttc ttttttttta tttgtttcca aattttttaa agttgttttt cgcatgaaat 3180

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 catatatatt tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa 3240

3181 gtatatataa ataatctgtt cttttcgtct aatttatcta tatgtaagct aattgctatt 3240

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtaaaatgta aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa 3300

3241 cattttacat tttagtgtcc taaaagcaca caccagaaga tgtgtctgtt ctactttgtt 3300

.......... .......... CEN6_ARS4 .......... .......... ..........

AseI(3308) .......... .......... .......... .......... ..........

3301 ttcggcatta atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc 3360

3301 aagccgtaat tatggactct cgtccttctc gttctatttt ccatcataaa caaccgctag 3360

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cccctagagt cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt 3420

3361 ggggatctca gaaaatgtag aagccttttg tttttgataa aaaagaaatt aaagaaaaaa 3420

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tactttctat ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat 3480

3421 atgaaagata aaaattaaat atataaatat aattttttaa atttaatatt aataaaaata 3480

.......... .......... CEN6_ARS4 .......... .......... ..........

PmlI(3485) .......... .......... .......... .......... ..........

3481 agcacgtgat gaaaagcctt agggtcaggt ggcacttttc ggggaaatgt gcgatgaacc 3540

3481 tcgtgcacta cttttcggaa tcccagtcca ccgtgaaaag cccctttaca cgctacttgg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cctatttgtt tatttttcta aatacattca aatatgtatc cgctaatgag acaataaccc 3600

3541 ggataaacaa ataaaaagat ttatgtaagt ttatacatag gcgattactc tgttattggg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgataaatgc ttcaattata ttgaaaaagg aagagtatga gtatccaaca tttccgtgtc 3660

3601 actatttacg aagttaatat aactttttcc ttctcatact cataggttgt aaaggcacag 3660

.......... .......... .......... .... Orf frame 1 Ampicillin(variant)

.......... .......... .......... .......... .......... ..........

3661 gcccttattc ccttttttgc ggccttctgc cttcctgttt ttgctcaccc agaaacgctg 3720

3661 cgggaataag ggaaaaaacg ccggaagacg gaaggacaaa aacgagtggg tctttgcgac 3720

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

3721 gtgaaagtaa aagatgctga agatcagttg ggtgcccgag tgggttacat tgaactggat 3780

3721 cactttcatt ttctacgact tctagtcaac ccacgggctc acccaatgta acttgaccta 3780

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctcaacagcg gcaaaatctt agagagtttt cgccccgaag aacgcttccc aatgatgagc 3840

3781 gagttgtcgc cgttttagaa tctctcaaaa gcggggcttc ttgcgaaggg ttactactcg 3840

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

3841 actttcaaag ttctgctatg tggcgcggtg ttatcccgta ttgatgctgg gcaagagcaa 3900

3841 tgaaagtttc aagacgatac accgcgccac aatagggcat aactacgacc cgttctcgtt 3900

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

3901 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtattcacc agtcacagaa 3960

3901 gagccagcgg cgtatgtgat aagagtctta ctgaaccaac tcataagtgg tcagtgtctt 3960

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

3961 aagcatctta cggatggcat gacagtaaga gaattatgta gtgctgccat aaccatgagt 4020

3961 ttcgtagaat gcctaccgta ctgtcattct cttaatacat cacgacggta ttggtactca 4020

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

4021 gataacactg cggccaactt acttctgaca acgattggag gaccgaagga gctaaccgct 4080

4021 ctattgtgac gccggttgaa tgaagactgt tgctaacctc ctggcttcct cgattggcga 4080

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

4081 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 4140

4081 aaaaacgtgt tgtaccccct agtacattga gcggaactag caacccttgg cctcgactta 4140

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

4141 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaactctc 4200

4141 cttcggtatg gtttgctgct cgcactgtgg tgctacggac atcgttaccg ttgttgagag 4200

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgcaaactat taactggcga actacttact cttgcttcac gccagcaact cattgactgg 4260

4201 gcgtttgata attgaccgct tgatgaatga gaacgaagtg cggtcgttga gtaactgacc 4260

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

4261 atggaggcgg acaaagttgc aggaccactt ctgcgctcgg cacttccggc tggctggttt 4320

4261 tacctccgcc tgtttcaacg tcctggtgaa gacgcgagcc gtgaaggccg accgaccaaa 4320

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

4321 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 4380

4321 taacgactat ttagacctcg gccactcgca cccagagcgc catagtaacg tcgtgacccc 4380

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 4440

4381 ggtctaccat tcgggagggc atagcatcaa tagatgtgct gcccctcagt ccgttgatac 4440

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

4441 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 4500

4441 ctacttgctt tatctgtcta gcgactctat ccacggagtg actaattcgt aaccattgac 4500

......... Orf frame 1 Ampicillin(variant) ......... ..........

.......... .......... .......... .......... .......... ..........

4501 tcagacgaag ggaaataaat agtagcccgc ctgatgtgcg ggcttttttt tggtggtggt 4560

4501 agtctgcttc cctttattta tcatcgggcg gactacacgc ccgaaaaaaa accaccacca 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 taccagtgtc gcttagctac cgtaggtcct cttaggcggt ccgattagcg acatagtcat 4620

4561 atggtcacag cgaatcgatg gcatccagga gaatccgcca ggctaatcgc tgtatcagta 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 caatgccttg acggcaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 4680

4621 gttacggaac tgccgtttta gggaattgca ctcaaaagca aggtgactcg cagtctgggg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4740

4681 catcttttct agtttcctag aagaactcta ggaaaaaaag acgcgcatta gacgacgaac 4740

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4741 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctactaact 4800

4741 gtttgttttt ttggtggcga tggtcgccac caaacaaacg gcctagttct cgatgattga 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 4860

4801 gaaaaaggct tccattgacc gaagtcgtct cgcgtctatg gtttatgaca agaagatcac 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 4920

4861 atcggcatca atccggtggt gaagttcttg agacatcgtg gcggatgtat ggagcgagac 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 4980

4921 gattaggaca atggtcaccg acgacggtca ccgctattca gcacagaatg gcccaacctg 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgctca 5040

4981 agttctgcta tcaatggcct attccgcgtc gccagcccga cttgcccccc aagcacgagt 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 5100

5041 gtcgggtcga acctcgcttg ctggatgtgg cttgactcta tggatgtcgc actcgatact 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 5160

5101 ctttcgcggt gcgaagggct tccctctttc cgcctgtcca taggccattc gccgtcccag 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 5220

5161 ccttgtcctc tcgcgtgctc cctcgaaggt ccccctttgc ggaccataga aatatcagga 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 5280

5221 cagcccaaag cggtggagac tgaactcgca gctaaaaaca ctacgagcag tccccccgcc 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 5340

5281 tcggatacct ttttgcggtc gttgcgccgg aaaaatgcca aggaccggaa aacgaccgga 5340

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 5400

5341 aaacgagtgt acaagaaagg acgcaatagg ggactaagac acctattggc ataatggcgg 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 5460

5401 aaactcactc gactatggcg agcggcgtcg gcttgctggc tcgcgtcgct cagtcactcg 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 5520

5461 ctccttcgcc ttctcgcggg ttatgcgttt ggcggagagg ggcgcgcaac cggctaagta 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgatgcag.. .......... .......... .......... .......... .......... 5528

5521 actacgtc.. .......... .......... .......... .......... .......... 5528

.......... .......... .......... .......... .......... ..........