Sequence of pDsRed-Express

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Sequence of pDsRed-Express

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Sequence of pDsRed-Express : 3311 BP; 795 A; 871 C; 889 G; 756 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gc caagcttg 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cggttcgaac 240

....... M13_pUC_rev_pri .... M13_reverse_pri ORF 1 .......

SalI BamHI SmaI271),AgeI(276),KpnI(277) NcoI(287)

241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggcctcctcc 300

241 gtacggacgt ccagctgaga tctcctaggg gcccatggcc agcggtggta ccggaggagg 300

.......... .......... ORF 1 .......... ....... DsRed2(variant)

.......... .......... .......... .......... .......... ..........

301 gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc 360

301 ctcctgcagt agttcctcaa gtacgcgaag ttccacgcgt acctcccgag gcacttgccg 360

.......... .... ORF 1 DsRed2(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 420

361 gtgctcaagc tctagctccc gctcccgctc ccggcgggga tgctcccgtg ggtctggcgg 420

.......... .... ORF 1 DsRed2(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag 480

421 ttcgacttcc actggttccc gccgggggac gggaagcgga ccctgtagga caggggggtc 480

.......... .... ORF 1 DsRed2(variant) .... ......... dsRed1_N_pri

.......... .......... .......... .......... .......... ..........

481 ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga ctacaagaag 540

481 aaggtcatgc cgaggttcca catgcacttc gtggggcggc tgtaggggct gatgttcttc 540

dsRed1_N_pri .. ORF 1 DsRed2(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 600

541 gacaggaagg ggctcccgaa gttcaccctc gcgcactact tgaagctcct gccgccgcac 600

.......... .... ORF 1 DsRed2(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtgaccgtga cccaggactc ctccctgcag gacggctcct tcatctacaa ggtgaagttc 660

601 cactggcact gggtcctgag gagggacgtc ctgccgagga agtagatgtt ccacttcaag 660

.......... .... ORF 1 DsRed2(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atcggcgtga acttcccctc cgacggcccc gtaatgcaga agaagactat gggctgggag 720

661 tagccgcact tgaaggggag gctgccgggg cattacgtct tcttctgata cccgaccctc 720

.......... .... ORF 1 DsRed2(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccacaaggcc 780

721 cggaggtggc tcgcggacat gggggcgctg ccgcacgact tcccgctcta ggtgttccgg 780

.......... .... ORF 1 DsRed2(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ctgaagctga aggacggcgg ccactacctg gtggagttca agtccatcta catggccaag 840

781 gacttcgact tcctgccgcc ggtgatggac cacctcaagt tcaggtagat gtaccggttc 840

.......... .... ORF 1 DsRed2(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aagcccgtgc agctgcccgg ctactactac gtggactcca agctggacat cacctcccac 900

841 ttcgggcacg tcgacgggcc gatgatgatg cacctgaggt tcgacctgta gtggagggtg 900

.......... .... ORF 1 DsRed2(variant) ... dsRed1_C_pri .........

.......... .......... .......... .......... .......... ..........

901 aacgaggact acaccatcgt ggagcagtac gagcgcgccg agggccgcca ccacctgttc 960

901 ttgctcctga tgtggtagca cctcgtcatg ctcgcgcggc tcccggcggt ggtggacaag 960

.......... .... ORF 1 DsRed2(variant) .... .......... ..........

NotI(967) .......... EcoRI(981) .......... .......... ..........

961 ctgtagcggc cgcgactcta gaattccaac tgagcgccgg tcgctaccat taccaacttg 1020

961 gacatcgccg gcgctgagat cttaaggttg actcgcggcc agcgatggta atggttgaac 1020

.......... .......... .......... .......... .......... ..........

.......... .......... SpeI(1045) .......... ApaI(1064) ..........

1021 tctggtgtca aaaataatag gcctactagt cggccgtacg ggccctttcg tctcgcgcgt 1080

1021 agaccacagt ttttattatc cggatgatca gccggcatgc ccgggaaagc agagcgcgca 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 1140

1081 aagccactac tgccactttt ggagactgtg tacgtcgagg gcctctgcca gtgtcgaaca 1140

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 1200

1141 gacattcgcc tacggccctc gtctgttcgg gcagtcccgc gcagtcgccc acaaccgccc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 1260

1201 acagccccga ccgaattgat acgccgtagt ctcgtctaac atgactctca cgtggtatac 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... AflII(1313)

1261 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg 1320

1261 gccacacttt atggcgtgtc tacgcattcc tcttttatgg cgtagtccgc cggaattccc 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 1380

1321 ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa gaatctgcag 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 1440

1381 tccaccgtga aaagcccctt tacacgcgcc ttggggataa acaaataaaa agatttatgt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 1500

1441 aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta ttataacttt 1500

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 1560

1501 ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa aacgccgtaa 1560

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

1561 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 1620

1561 aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac gacttctagt 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 1680

1621 caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct aggaactctc 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 1740

1681 aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg atacaccgcg 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 1800

1741 ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg tgataagagt 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 1860

1801 cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac cgtactgtca 1860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 1920

1861 ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt tgaatgaaga 1920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 1980

1921 ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc ccctagtaca 1980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 2040

1981 ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc tgctcgcact 2040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 2100

2041 gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac cgcttgatga 2100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 2160

2101 atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc aacgtcctgg 2160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 2220

2161 tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac ctcggccact 2220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 2280

2221 cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga gggcatagca 2280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 2340

2281 tcaatagatg tgctgcccct cagtccgttg atacctactt gctttatctg tctagcgact 2340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 2400

2341 ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga gtatatatga 2400

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 2460

2401 aatctaacta aattttgaag taaaaattaa attttcctag atccacttct aggaaaaact 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 2520

2461 attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca gtctggggca 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 2580

2521 tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga cgacgaacgt 2580

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 2640

2581 ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg atggttgaga 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 2700

2641 aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacagg aagatcacat 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 2760

2701 cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg agcgagacga 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 2820

2761 ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc ccaacctgag 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 2880

2821 ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa gcacgtgtgt 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 2940

2881 cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac tcgatactct 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 3000

2941 ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc cgtcccagcc 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 3060

3001 ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa tatcaggaca 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 3120

3061 gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc cccccgcctc 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 3180

3121 ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa cgaccggaaa 3180

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 3240

3181 acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat aatggcggaa 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 3300

3241 actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca gtcactcgct 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggaagcggaa g......... .......... .......... .......... .......... 3311

3301 ccttcgcctt c......... .......... .......... .......... .......... 3311

.......... .......... .......... .......... .......... ..........