Sequence of pECFP-C1

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Sequence of pECFP-C1

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Sequence of pECFP-C1 : 4731 BP; 1138 A; 1286 C; 1244 G; 1063 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro .. CMV_fwd_pri CMV_pro .....

.......... .......... .......... .......... ..... NheI(591) AgeI(600)

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 660

601 ggccagcggt ggtaccactc gttcccgctc ctcgacaagt ggccccacca cgggtaggac 660

.......... .......... ORF 1 ECFP ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 720

661 cagctcgacc tgccgctgca tttgccggtg ttcaagtcgc acaggccgct cccgctcccg 720

EGFP_N_pri ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 780

721 ctacggtgga tgccgttcga ctgggacttc aagtagacgt ggtggccgtt cgacgggcac 780

.......... ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc 840

781 gggaccgggt gggagcactg gtgggactgg accccgcacg tcacgaagtc ggcgatgggg 840

.......... ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 900

841 ctggtgtact tcgtcgtgct gaagaagttc aggcggtacg ggcttccgat gcaggtcctc 900

.......... ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 960

901 gcgtggtaga agaagttcct gctgccgttg atgttctggg cgcggctcca cttcaagctc 960

.......... ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1020

961 ccgctgtggg accacttggc gtagctcgac ttcccgtagc tgaagttcct cctgccgttg 1020

.......... ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac 1080

1021 taggaccccg tgttcgacct catgttgatg tagtcggtgt tgcagatata gtggcggctg 1080

.......... ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc 1140

1081 ttcgtcttct tgccgtagtt ccggttgaag ttctaggcgg tgttgtagct cctgccgtcg 1140

.......... ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1200

1141 cacgtcgagc ggctggtgat ggtcgtcttg tgggggtagc cgctgccggg gcacgacgac 1200

.......... ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1260

1201 gggctgttgg tgatggactc gtgggtcagg cgggactcgt ttctggggtt gctcttcgcg 1260

.......... ....... ORF 1 ECFP ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1320

1261 ctagtgtacc aggacgacct caagcactgg cggcggccct agtgagagcc gtacctgctc 1320

....... EGFP_C_pri ORF 1 ECFP ORF 1 ....... .......... ..........

BglII,XhoI SacI,HindIII EcoRI PstI368),SalI(1369)

1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc 1380

1321 gacatgttca ggcctgagtc tagagctcga gttcgaagct taagacgtca gctgccatgg 1380

ECFP ......... ORF 1 ORF 1 ......... .......... ..........

SacII,ApaI,SmaI,BamHI(1390) XbaI(1402) BclI(1412)

1381 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta 1440

1381 cgcccgggcc ctaggtggcc tagatctatt gactagtatt agtcggtatg gtgtaaacat 1440

.......... ORF 1 ORF 1 ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

1441 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 1500

1441 ctccaaaatg aacgaaattt tttggagggt gtggaggggg acttggactt tgtattttac 1500

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 1560

1501 ttacgttaac aacaacaatt gaacaaataa cgtcgaatat taccaatgtt tatttcgtta 1560

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 1620

1561 tcgtagtgtt taaagtgttt atttcgtaaa aaaagtgacg taagatcaac accaaacagg 1620

.......... ......... SV40_PA_term ......... ......... EBV_rev_pri

......... AflII(1637) ......... .......... .......... ..........

1621 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc 1680

1621 tttgagtagt tacatagaat tccgcattta acattcgcaa ttataaaaca attttaagcg 1680

SV40_PA_term EBV_rev_pri ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 1740

1681 caatttaaaa acaatttagt cgagtaaaaa attggttatc cggctttagc cgttttaggg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 1800

1741 aatatttagt tttcttatct ggctctatcc caactcacaa caaggtcaaa ccttgttctc 1800

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

1801 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 1860

1801 aggtgataat ttcttgcacc tgaggttgca gtttcccgct ttttggcaga tagtcccgct 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 1920

1861 accgggtgat gcacttggta gtgggattag ttcaaaaaac cccagctcca cggcatttcg 1920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 1980

1921 tgatttagcc ttgggatttc cctcgggggc taaatctcga actgcccctt tcggccgctt 1980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 2040

1981 gcaccgctct ttccttccct tctttcgctt tcctcgcccg cgatcccgcg accgttcaca 2040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 2100

2041 tcgccagtgc gacgcgcatt ggtggtgtgg gcggcgcgaa ttacgcggcg atgtcccgcg 2100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 2160

2101 cagtccaccg tgaaaagccc ctttacacgc gccttgggga taaacaaata aaaagattta 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 2220

2161 tgtaagttta tacataggcg agtactctgt tattgggact atttacgaag ttattataac 2220

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt 2280

2221 tttttccttc tcaggactcc gcctttcttg gtcgacacct tacacacagt caatcccaca 2280

.......... .......... .......... ......... pBABE_3_pri SV40_enh

.......... .......... .......... .......... .......... ..........

2281 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 2340

2281 cctttcaggg gtccgagggg tcgtccgtct tcatacgttt cgtacgtaga gttaatcagt 2340

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 2400

2341 cgttggtcca cacctttcag gggtccgagg ggtcgtccgt cttcatacgt ttcgtacgta 2400

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 2460

2401 gagttaatca gtcgttggta tcagggcggg gattgaggcg ggtagggcgg ggattgaggc 2460

.......... ...... SV40_enh SV40_pro ...... ......... SV40_origin

.......... .......... .......... .......... .......... ..........

2461 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 2520

2461 gggtcaaggc gggtaagagg cggggtaccg actgattaaa aaaaataaat acgtctccgg 2520

SV40_enh ..... SV40_pro SV40_origin ..... ........ SV40pro_F_pri

.......... .......... .......... .......... .......... ..........

2521 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 2580

2521 ctccggcgga gccggagact cgataaggtc ttcatcactc ctccgaaaaa acctccggat 2580

.......... SV40_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 2640

2581 ccgaaaacgt ttctagctag ttctctgtcc tactcctagc aaagcgtact aacttgttct 2640

.......... .......... .......... .......... ........ ORF 2 Neomycin

.......... .......... EagI(2661) .......... .......... ..........

2641 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 2700

2641 acctaacgtg cgtccaagag gccggcgaac ccacctctcc gataagccga tactgacccg 2700

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 2760

2701 tgttgtctgt tagccgacga gactacggcg gcacaaggcc gacagtcgcg tccccgcggg 2760

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc 2820

2761 ccaagaaaaa cagttctggc tggacaggcc acgggactta cttgacgttc tgctccgtcg 2820

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... MscI(2837) .......... FspI(2857) .......... ..........

2821 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 2880

2821 cgccgatagc accgaccggt gctgcccgca aggaacgcgt cgacacgagc tgcaacagtg 2880

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 2940

2881 acttcgccct tccctgaccg acgataaccc gcttcacggc cccgtcctag aggacagtag 2940

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 3000

2941 agtggaacga ggacggctct ttcataggta gtaccgacta cgttacgccg ccgacgtatg 3000

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 3060

3001 cgaactaggc cgatggacgg gtaagctggt ggttcgcttt gtagcgtagc tcgctcgtgc 3060

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 3120

3061 atgagcctac cttcggccag aacagctagt cctactagac ctgcttctcg tagtccccga 3120

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt 3180

3121 gcgcggtcgg cttgacaagc ggtccgagtt ccgctcgtac gggctgccgc tcctagagca 3180

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 3240

3181 gcactgggta ccgctacgga cgaacggctt atagtaccac cttttaccgg cgaaaagacc 3240

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 3300

3241 taagtagctg acaccggccg acccacaccg cctggcgata gtcctgtatc gcaaccgatg 3300

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 3360

3301 ggcactataa cgacttctcg aaccgccgct tacccgactg gcgaaggagc acgaaatgcc 3360

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 3420

3361 atagcggcga gggctaagcg tcgcgtagcg gaagatagcg gaagaactgc tcaagaagac 3420

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 3480

3421 tcgccctgag accccaagct ttactggctg gttcgctgcg ggttggacgg tagtgctcta 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 3540

3481 aagctaaggt ggcggcggaa gatactttcc aacccgaagc cttagcaaaa ggccctgcgg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg 3600

3541 ccgacctact aggaggtcgc gcccctagag tacgacctca agaagcgggt gggatccccc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata 3660

3601 tccgattgac tttgtgcctt cctctgttat ggccttcctt gggcgcgata ctgccgttat 3660

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc 3720

3661 ttttctgtct tattttgcgt gccacaaccc agcaaacaag tatttgcgcc ccaagccagg 3720

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt 3780

3721 gtcccgaccg tgagacagct atggggtggc tctggggtaa ccccggttat gcgggcgcaa 3780

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac 3840

3781 agaaggaaaa ggggtggggt ggggggttca agcccacttc cgggtcccga gcgtcggttg 3840

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta 3900

3841 cagccccgcc gtccgggacg gtatcggagt ccaatgagta tatatgaaat ctaactaaat 3900

.......... TK_PA_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 3960

3901 tttgaagtaa aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 4020

3961 ttttagggaa ttgcactcaa aagcaaggtg actcgcagtc tggggcatct tttctagttt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 4080

4021 cctagaagaa ctctaggaaa aaaagacgcg cattagacga cgaacgtttg tttttttggt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 4140

4081 ggcgatggtc gccaccaaac aaacggccta gttctcgatg gttgagaaaa aggcttccat 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 4200

4141 tgaccgaagt cgtctcgcgt ctatggttta tgacaggaag atcacatcgg catcaatccg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 4260

4201 gtggtgaagt tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 4320

4261 caccgacgac ggtcaccgct attcagcaca gaatggccca acctgagttc tgctatcaat 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... ......... ApaLI(4359) ......... ..........

4321 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 4380

4321 ggcctattcc gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 4440

4381 gcttgctgga tgtggcttga ctctatggat gtcgcactcg atactctttc gcggtgcgaa 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 4500

4441 gggcttccct ctttccgcct gtccataggc cattcgccgt cccagccttg tcctctcgcg 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 4560

4501 tgctccctcg aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 4620

4561 gagactgaac tcgcagctaa aaacactacg agcagtcccc ccgcctcgga tacctttttg 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 4680

4621 cggtcgttgc gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg agtgtacaag 4680

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t......... 4731

4681 aaaggacgca ataggggact aagacaccta ttggcataat ggcggtacgt a......... 4731

.......... .......... .......... .......... .......... ..........