Sequence of pECFP

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Sequence of pECFP

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Sequence of pECFP : 3355 BP; 827 A; 889 C; 888 G; 751 T; 0 other;

.......... .......... .......... .......... .......... PvuII(55)

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gc caagcttg 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cggttcgaac 240

....... M13_pUC_rev_pri .... M13_reverse_pri ORF 1 .......

PstI,SalI BamHI SmaI,AgeI276),KpnI(277) NcoI(287)

241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgagcaag 300

241 gtacggacgt ccagctgaga tctcctaggg gcccatggcc agcggtggta ccactcgttc 300

.......... .......... ORF 1 .......... .......... ECFP

.......... .......... .......... .......... .......... ..........

301 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 360

301 ccgctcctcg acaagtggcc ccaccacggg taggaccagc tcgacctgcc gctgcatttg 360

.......... .......... ORF 1 ECFP .......... EGFP_N_pri ..........

.......... .......... .......... .......... .......... ..........

361 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 420

361 ccggtgttca agtcgcacag gccgctcccg ctcccgctac ggtggatgcc gttcgactgg 420

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 480

421 gacttcaagt agacgtggtg gccgttcgac gggcacggga ccgggtggga gcactggtgg 480

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 540

481 gactggaccc cgcacgtcac gaagtcggcg atggggctgg tgtacttcgt cgtgctgaag 540

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 600

541 aagttcaggc ggtacgggct tccgatgcag gtcctcgcgt ggtagaagaa gttcctgctg 600

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 660

601 ccgttgatgt tctgggcgcg gctccacttc aagctcccgc tgtgggacca cttggcgtag 660

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 720

661 ctcgacttcc cgtagctgaa gttcctcctg ccgttgtagg accccgtgtt cgacctcatg 720

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc 780

721 ttgatgtagt cggtgttgca gatatagtgg cggctgttcg tcttcttgcc gtagttccgg 780

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 840

781 ttgaagttct aggcggtgtt gtagctcctg ccgtcgcacg tcgagcggct ggtgatggtc 840

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 900

841 gtcttgtggg ggtagccgct gccggggcac gacgacgggc tgttggtgat ggactcgtgg 900

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 960

901 gtcaggcggg actcgtttct ggggttgctc ttcgcgctag tgtaccagga cgacctcaag 960

.......... .......... ORF 1 ECFP .......... .......... EGFP_C_pri

.......... .......... .......... .......... .......... ..........

961 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac 1020

961 cactggcggc ggccctagtg agagccgtac ctgctcgaca tgttcatttc gccggcgctg 1020

.......... .......... ORF 1 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tctagaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata 1080

1021 agatcttaag gttgactcgc ggccagcgat ggtaatggtt gaacagacca cagtttttat 1080

.......... .......... .......... .......... .......... ..........

StuI(1085),SpeI(1089) ApaI(1108) ........ .......... ..........

1081 ataggcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg 1140

1081 tatccggatg atcagccggc atgcccggga aagcagagcg cgcaaagcca ctactgccac 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 1200

1141 ttttggagac tgtgtacgtc gagggcctct gccagtgtcg aacagacatt cgcctacggc 1200

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 1260

1201 cctcgtctgt tcgggcagtc ccgcgcagtc gcccacaacc gcccacagcc ccgaccgaat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 1320

1261 tgatacgccg tagtctcgtc taacatgact ctcacgtggt atacgccaca ctttatggcg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta 1380

1321 tgtctacgca ttcctctttt atggcgtagt ccgccggaat tcccggagca ctatgcggat 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AatII(1423) .........

1381 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 1440

1381 aaaaatatcc aattacagta ctattattac caaagaatct gcagtccacc gtgaaaagcc 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 1500

1441 cctttacacg cgccttgggg ataaacaaat aaaaagattt atgtaagttt atacataggc 1500

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

1501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 1560

1501 gagtactctg ttattgggac tatttacgaa gttattataa ctttttcctt ctcatactca 1560

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 1620

1561 taagttgtaa aggcacagcg ggaataaggg aaaaaacgcc gtaaaacgga aggacaaaaa 1620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 1680

1621 cgagtgggtc tttgcgacca ctttcatttt ctacgacttc tagtcaaccc acgtgctcac 1680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 1740

1681 ccaatgtagc ttgacctaga gttgtcgcca ttctaggaac tctcaaaagc ggggcttctt 1740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 1800

1741 gcaaaaggtt actactcgtg aaaatttcaa gacgatacac cgcgccataa tagggcataa 1800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1860

1801 ctgcggcccg ttctcgttga gccagcggcg tatgtgataa gagtcttact gaaccaactc 1860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1920

1861 atgagtggtc agtgtctttt cgtagaatgc ctaccgtact gtcattctct taatacgtca 1920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1980

1921 cgacggtatt ggtactcact attgtgacgc cggttgaatg aagactgttg ctagcctcct 1980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 2040

1981 ggcttcctcg attggcgaaa aaacgtgttg taccccctag tacattgagc ggaactagca 2040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 2100

2041 acccttggcc tcgacttact tcggtatggt ttgctgctcg cactgtggtg ctacggacat 2100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... FspI(2119) .......... .......... .......... ..........

2101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 2160

2101 cgttaccgtt gttgcaacgc gtttgataat tgaccgcttg atgaatgaga tcgaagggcc 2160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 2220

2161 gttgttaatt atctgaccta cctccgccta tttcaacgtc ctggtgaaga cgcgagccgg 2220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 2280

2221 gaaggccgac cgaccaaata acgactattt agacctcggc cactcgcacc cagagcgcca 2280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 2340

2281 tagtaacgtc gtgaccccgg tctaccattc gggagggcat agcatcaata gatgtgctgc 2340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 2400

2341 ccctcagtcc gttgatacct acttgcttta tctgtctagc gactctatcc acggagtgac 2400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 2460

2401 taattcgtaa ccattgacag tctggttcaa atgagtatat atgaaatcta actaaatttt 2460

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 2520

2461 gaagtaaaaa ttaaattttc ctagatccac ttctaggaaa aactattaga gtactggttt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 2580

2521 tagggaattg cactcaaaag caaggtgact cgcagtctgg ggcatctttt ctagtttcct 2580

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

2581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2640

2581 agaagaactc taggaaaaaa agacgcgcat tagacgacga acgtttgttt ttttggtggc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2700

2641 gatggtcgcc accaaacaaa cggcctagtt ctcgatggtt gagaaaaagg cttccattga 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 2760

2701 ccgaagtcgt ctcgcgtcta tggtttatga caggaagatc acatcggcat caatccggtg 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2820

2761 gtgaagttct tgagacatcg tggcggatgt atggagcgag acgattagga caatggtcac 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2880

2821 cgacgacggt caccgctatt cagcacagaa tggcccaacc tgagttctgc tatcaatggc 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2940

2881 ctattccgcg tcgccagccc gacttgcccc ccaagcacgt gtgtcgggtc gaacctcgct 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 3000

2941 tgctggatgt ggcttgactc tatggatgtc gcactcgata ctctttcgcg gtgcgaaggg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 3060

3001 cttccctctt tccgcctgtc cataggccat tcgccgtccc agccttgtcc tctcgcgtgc 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 3120

3061 tccctcgaag gtcccccttt gcggaccata gaaatatcag gacagcccaa agcggtggag 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 3180

3121 actgaactcg cagctaaaaa cactacgagc agtccccccg cctcggatac ctttttgcgg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 3240

3181 tcgttgcgcc ggaaaaatgc caaggaccgg aaaacgaccg gaaaacgagt gtacaagaaa 3240

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 3300

3241 ggacgcaata ggggactaag acacctattg gcataatggc ggaaactcac tcgactatgg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag..... 3355

3301 cgagcggcgt cggcttgctg gctcgcgtcg ctcagtcact cgctccttcg ccttc..... 3355

.......... .......... .......... .......... .......... ..........