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pEF-GFP
Gene/insert name: EF1 alpha promoter
Alternative names: EF1A1 promoter
eukaryotic translation elongation factor 1 alpha 1 EF1A1 Insert size (bp): 1188
Gene/insert aliases: EEF1A1, CCS3, EF1A, PTI1, CCS-3, EEF-1, EEF1A, EF-Tu, LENG7, eEF1A-1, FLJ25721, GRAF-1EF, MGC16224, MGC102687, MGC131894, HNGC:16303 Species of gene(s): H. sapiens (human)
Fusion proteins or tags: EGFP
Terminal: C terminal on backbone
Vector backbone: pCAGEN
Type of vector: Mammalian expression
Backbone size (bp): 3086
Cloning site 5': SpeI
Site destroyed during cloning: Yes
Cloning site 3': NotI
Site destroyed during cloning: No
5' Sequencing primer: na (List of Sequencing Primers)
3' Sequencing primer: EGFP-N
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Article: Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T et al. (Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. Pubmed)