Sequence of pEF-GFP

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pEF-GFP

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pEF-GFP : 5051 BP; 1143 A; 1344 C; 1321 G; 1243 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtcgacattg attattgact agatcatcgc gtgaggctcc ggtgcccgtc agtgggcaga 60

1 cagctgtaac taataactga tctagtagcg cactccgagg ccacgggcag tcacccgtct 60

.......... .......... .......... ........ EF1a_promoter ........

.......... .......... .......... .......... .......... ..........

61 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 120

61 cgcgtgtagc gggtgtcagg ggctcttcaa cccccctccc cagccgttaa cttggccacg 120

.......... ........ EF1a_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

121 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 180

121 gatctcttcc accgcgcccc atttgaccct ttcactacag cacatgaccg aggcggaaaa 180

.......... ........ EF1a_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

181 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 240

181 agggctccca ccccctcttg gcatatattc acgtcatcag cggcacttgc aagaaaaagc 240

.......... ........ EF1a_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

241 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 300

241 gttgcccaaa cggcggtctt gtgtccattc acggcacaca ccaagggcgc ccggaccgga 300

.......... ........ EF1a_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

301 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg 360

301 gaaatgccca ataccgggaa cgcacggaac ttaatgaagg tgcggggacc gacgtcatgc 360

.......... ....... EF1a_promoter Orf frame 1 ....... ..........

.......... .......... .......... .......... .......... AflII(418)

361 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 420

361 actaagaact agggctcgaa gcccaacctt cacccaccct ctcaagctcc ggaacgcgaa 420

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... .......... .......... .......... .......... ..........

421 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 480

421 ttcctcgggg aagcggagca cgaactcaac tccggaccgg acccgcgacc ccggcggcgc 480

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 540

481 acgcttagac caccgtggaa gcgcggacag agcgacgaaa gctattcaga gatcggtaaa 540

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 600

541 ttttaaaaac tactggacga cgctgcgaaa aaaagaccgt tctatcagaa catttacgcc 600

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... .......... .......... .......... .......... ..........

601 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 660

601 cggttctaga cgtgtgacca taaagccaaa aaccccggcg cccgccgctg ccccgggcac 660

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 720

661 gcagggtcgc gtgtacaagc cgctccgccc cggacgctcg cgccggtggc tcttagcctg 720

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... .......... .......... .......... .......... ..........

721 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg 780

721 cccccatcag agttcgaccg gccggacgag accacggacc ggagcgcggc ggcacatagc 780

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 840

781 ggggcgggac ccgccgttcc gaccgggcca gccgtggtca acgcactcgc ctttctaccg 840

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... .......... SacI(867) .......... .......... ..........

841 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 900

841 gcgaagggcc gggacgacgt ccctcgagtt ttacctcctg cgccgcgagc cctctcgccc 900

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... .......... .......... .......... .......... ..........

901 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 960

901 gcccactcag tgggtgtgtt tccttttccc ggaaaggcag gagtcggcag cgaagtacac 960

.......... .. EF1a_promoter Orf frame 1 .. .......... ..........

.......... NarI(978) .......... .......... XhoI(1004) ..........

961 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 1020

961 tgaggtgcct catggcccgc ggcaggtccg tggagctaat caagagctcg aaaacctcat 1020

.......... ........ EF1a_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 1080

1021 gcagcagaaa tccaaccccc ctccccaaaa tacgctacct caaaggggtg tgactcaccc 1080

.......... ........ EF1a_promoter ORF3 ........ ..........

.......... .......... .......... .......... .......... ..........

1081 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 1140

1081 acctctgact tcaatccggt cgaaccgtga actacattaa gaggaacctt aaacgggaaa 1140

.......... ...... EF1a_promoter ORF3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 1200

1141 aactcaaacc tagaaccaag taagagttcg gagtctgtca ccaagtttca aaaaaagaag 1200

.......... ...... EF1a_promoter ORF3 EF1a_f_pri ...... ..........

......... EcoRI(1219) ......... KpnI(1239) SmaI(1248) ..........

1201 catttcaggt gtcgtgagga attctgcagt cgacggtacc gcgggcccgg gatccaccgg 1260

1201 gtaaagtcca cagcactcct taagacgtca gctgccatgg cgcccgggcc ctaggtggcc 1260

EF1a_promoter ...... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 1320

1261 agcggtggta ccactcgttc ccgctcctcg acaagtggcc ccaccacggg taggaccagc 1320

.......... ....... ORF3 EGFP Orf frame 3 ....... ..........

.......... .......... .......... .......... .......... ..........

1321 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 1380

1321 tcgacctgcc gctgcatttg ccggtgttca agtcgcacag gccgctcccg ctcccgctac 1380

EGFP_N_pri .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 1440

1381 ggtggatgcc gttcgactgg gacttcaagt agacgtggtg gccgttcgac gggcacggga 1440

.......... .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 1500

1441 ccgggtggga gcactggtgg gactggatgc cgcacgtcac gaagtcggcg atggggctgg 1500

.......... .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 1560

1501 tgtacttcgt cgtgctgaag aagttcaggc ggtacgggct tccgatgcag gtcctcgcgt 1560

.......... .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 1620

1561 ggtagaagaa gttcctgctg ccgttgatgt tctgggcgcg gctccacttc aagctcccgc 1620

.......... .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 1680

1621 tgtgggacca cttggcgtag ctcgacttcc cgtagctgaa gttcctcctg ccgttgtagg 1680

.......... .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 1740

1681 accccgtgtt cgacctcatg ttgatgttgt cggtgttgca gatatagtac cggctgttcg 1740

.......... .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 1800

1741 tcttcttgcc gtagttccac ttgaagttct aggcggtgtt gtagctcctg ccgtcgcacg 1800

.......... .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 1860

1801 tcgagcggct ggtgatggtc gtcttgtggg ggtagccgct gccggggcac gacgacgggc 1860

.......... .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 1920

1861 tgttggtgat ggactcgtgg gtcaggcggg actcgtttct ggggttgctc ttcgcgctag 1920

.......... .... ORF3 EGFP Orf frame 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 1980

1921 tgtaccagga cgacctcaag cactggcggc ggccctagtg agagccgtac ctgctcgaca 1980

.. EGFP_C_primer ORF3 EGFP Orf frame 3 .. .......... ..........

.... EagI(1991),NotI(1991) .... .......... .......... ..........

1981 acaagtaaag cggccgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg 2040

1981 tgttcatttc gccggcgtga ggagtccacg tccgacggat agtcttccac caccgaccac 2040

.......... .......... ORF3 ....... rb_glob_PA_term .......

MscI(2043) .......... .......... .......... .......... ..........

2041 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg 2100

2041 accggttacg ggaccgagtg tttatggtga ctctagaaaa agggagacgg tttttaatac 2100

.......... ..... ORF3 rb_glob_PA_term ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca 2160

2101 ccctgtagta cttcggggaa ctcgtagact gaagaccgat tatttccttt aaataaaagt 2160

....... ORF3 rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa 2220

2161 aacgttatca cacaacctta aaaaacacag agagtgagcc ttcctgtata ccctcccgtt 2220

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gccatatgct 2280

2221 tagtaaattt tgtagtctta ctcataaacc aaatctcaaa ccgttgtata cggtatacga 2280

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggctgccatg aacaaaggtg gctataaaga ggtcatcagt atatgaaaca gccccctgct 2340

2281 ccgacggtac ttgtttccac cgatatttct ccagtagtca tatactttgt cgggggacga 2340

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt 2400

2341 caggtaagga ataaggtatc ttttcggaac tgaactccaa tctaaaaaaa atataaaaca 2400

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga 2460

2401 aaacacaata aaaaaagaaa ttgtagggat tttaaaagga atgtacaaaa tgatcggtct 2460

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atgaagatcc 2520

2461 aaaaaggagg agaggactga tgagggtcag tatcgacagg gagaagagaa tacttctagg 2520

.......... ....... rb_glob_PA_term ....... .......... ..........

........ HindIII(2536) ........ .......... .......... ..........

2521 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 2580

2521 gagctggacg tcgggttcga accgcattag taccagtatc gacaaaggac acactttaac 2580

.......... .......... .......... M13_r .......... M1_pUC_r

.......... .......... .......... .......... .......... ..........

2581 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 2640

2581 aataggcgag tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc 2640

M1_pUC_r .......... ......... lac_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

2641 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 2700

2641 acggattact cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc 2760

2701 ccctttggac agcacggtcg cctaggcgta gagttaatca gtcgttggta tcagggcggg 2760

.......... .......... .......... ......... SV4_promoter .........

.......... .......... .......... .......... .......... ..........

2761 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 2820

2761 gattgaggcg ggtagggcgg ggattgaggc gggtcaaggc gggtaagagg cggggtaccg 2820

.......... ........ SV4_promoter SV40_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2821 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 2880

2821 actgattaaa aaaaataaat acgtctccgg ctccggcgga gccggagact cgataaggtc 2880

. SV40_origin SV4_promoter SV4pro_F_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg 2940

2881 ttcatcactc ctccgaaaaa acctccggat ccgaaaacgt ttttcgattg aacaaataac 2940

......... SV4_promoter ......... .......... ......... SV4_PA_term

.......... .......... .......... .......... .......... ..........

2941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 3000

2941 gtcgaatatt accaatgttt atttcgttat cgtagtgttt aaagtgttta tttcgtaaaa 3000

.......... ......... SV4_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 3060

3001 aaagtgacgt aagatcaaca ccaaacaggt ttgagtagtt acatagaata gtacagacct 3060

.......... .. SV4_PA_term EBV_rev_primer .. .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 3120

3061 aggcgacgta attacttagc cggttgcgcg cccctctccg ccaaacgcat aacccgcgag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 3180

3121 aaggcgaagg agcgagtgac tgagcgacgc gagccagcaa gccgacgccg ctcgccatag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 3240

3181 tcgagtgagt ttccgccatt atgccaatag gtgtcttagt cccctattgc gtcctttctt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 3300

3241 gtacactcgt tttccggtcg ttttccggtc cttggcattt ttccggcgca acgaccgcaa 3300

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 3360

3301 aaaggtatcc gaggcggggg gactgctcgt agtgttttta gctgcgagtt cagtctccac 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 3420

3361 cgctttgggc tgtcctgata tttctatggt ccgcaaaggg ggaccttcga gggagcacgc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 3480

3421 gagaggacaa ggctgggacg gcgaatggcc tatggacagg cggaaagagg gaagcccttc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 3540

3481 gcaccgcgaa agagttacga gtgcgacatc catagagtca agccacatcc agcaagcgag 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 3600

3541 gttcgacccg acacacgtgc ttggggggca agtcgggctg gcgacgcgga ataggccatt 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 3660

3601 gatagcagaa ctcaggttgg gccattctgt gctgaatagc ggtgaccgtc gtcggtgacc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 3720

3661 attgtcctaa tcgtctcgct ccatacatcc gccacgatgt ctcaagaact tcaccaccgg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 3780

3721 attgatgccg atgtgatctt cctgtcataa accatagacg cgagacgact tcggtcaatg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 3840

3781 gaagcctttt tctcaaccat cgagaactag gccgtttgtt tggtggcgac catcgccacc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 3900

3841 aaaaaaacaa acgttcgtcg tctaatgcgc gtcttttttt cctagagttc ttctaggaaa 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 3960

3901 ctagaaaaga tgccccagac tgcgagtcac cttgcttttg agtgcaattc cctaaaacca 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 4020

3961 gtactctaat agtttttcct agaagtggat ctaggaaaat ttaattttta cttcaaaatt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 4080

4021 tagttagatt tcatatatac tcatttgaac cagactgtca atggttacga attagtcact 4080

.......... .......... .......... .... Orf frame 1 Ampicillin ....

.......... .......... .......... .......... .......... ..........

4081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 4140

4081 ccgtggatag agtcgctaga cagataaagc aagtaggtat caacggactg aggggcagca 4140

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 4200

4141 catctattga tgctatgccc tcccgaatgg tagaccgggg tcacgacgtt actatggcgc 4200

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 4260

4201 tctgggtgcg agtggccgag gtctaaatag tcgttatttg gtcggtcggc cttcccggct 4260

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 4320

4261 cgcgtcttca ccaggacgtt gaaataggcg gaggtaggtc agataattaa caacggccct 4320

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... FspI(4355) .......... ..........

4321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 4380

4321 tcgatctcat tcatcaagcg gtcaattatc aaacgcgttg caacaacggt aacgatgtcc 4380

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 4440

4381 gtagcaccac agtgcgagca gcaaaccata ccgaagtaag tcgaggccaa gggttgctag 4440

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 4500

4441 ttccgctcaa tgtactaggg ggtacaacac gttttttcgc caatcgagga agccaggagg 4500

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 4560

4501 ctagcaacag tcttcattca accggcgtca caatagtgag taccaatacc gtcgtgacgt 4560

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 4620

4561 attaagagaa tgacagtacg gtaggcattc tacgaaaaga cactgaccac tcatgagttg 4620

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 4680

4621 gttcagtaag actcttatca catacgccgc tggctcaacg agaacgggcc gcagttatgc 4680

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 4740

4681 cctattatgg cgcggtgtat cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag 4740

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 4800

4741 ccccgctttt gagagttcct agaatggcga caactctagg tcaagctaca ttgggtgagc 4800

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 4860

4801 acgtgggttg actagaagtc gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg 4860

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 4920

4861 tccttccgtt ttacggcgtt ttttccctta ttcccgctgt gcctttacaa cttatgagta 4920

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 4980

4921 tgagaaggaa aaagttataa taacttcgta aatagtccca ataacagagt actcgcctat 4980

.......... .......... .......... .......... ........ AmpR_promoter

.......... .......... .......... .......... .......... ..........

4981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 5040

4981 gtataaactt acataaatct ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt 5040

AmpR_promoter ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 agtgccacct g......... .......... .......... .......... .......... 5051

5041 tcacggtgga c......... .......... .......... .......... .......... 5051

.......... .......... .......... .......... .......... ..........