Sequence of pENTR1A

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Sequence of pENTR1A

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Sequence of pENTR1A : 2717 BP; 710 A; 602 C; 671 G; 734 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcgatcgt acctagagcc 60

.......... .......... .......... .......... .......... ..........

AatII(66) .......... .......... .......... .......... ..........

61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 120

61 cctgcagatt gatgattcgc tctcatccct tgacggtccg tagtttattt tgctttccga 120

.......... .......... .......... .......... ......... rrnB_T1_term

.......... .......... .......... .......... .......... ..........

121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 180

121 gtcagccttc tgacccggaa agcaaaatag acaacaaaca gccacttgcg agaggactca 180

......... rrnB_T1_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 240

181 tcctgtttag gcggccctcg cctaaacttg caacacttcg ttgccgggcc tcccaccgcc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 300

241 cgtcctgcgg gcggtatttg acggtccgta gtttgattcg tcttccggta ggactgccta 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 360

301 ccggaaaaac gcaaagatgt ttgagaagga caatcaatca atgaattcga gcccggggtt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 420

361 tattactaaa ataaaactga ctatcactgg acaagcaacg ttgtttaact attcgttacg 420

.......... ......... attL2 attL1 ......... .......... ..........

.......... .......... .......... DraI(459) .......... ..........

421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac 480

421 aaaaaatatt acggttgaaa catgtttttt cgtccgaaat ttccttggtt aagtcagctg 480

......... attL2 attL1 ......... .......... .......... ..........

BamHI(482) KpnI(492) .......... .......... .......... ..........

481 tggatccggt accgaattcg cttactaaaa gccagataac agtatgcgta tttgcgcgct 540

481 acctaggcca tggcttaagc gaatgatttt cggtctattg tcatacgcat aaacgcgcga 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt 600

541 ctaaaaacgc catattctta tatatgacta tacatatggg cttcatacag tttttctcca 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtgcttctag aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt 660

601 cacgaagatc ttacgtcaaa ttccaaatgt ggatattttc tctctcggca atagcagaca 660

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... SmaI(695) .......... ..........

661 ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatagtg atccccctgg 720

661 aacacctaca tgtctcacta taataactgt gcgggcccgc tgcctatcac tagggggacc 720

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg 780

721 ggtcacgtgc agacgacagt ctatttcaga gggcacttga aatgggccac cacgtatagc 780

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg 840

781 ccctactttc gaccgcgtac tactggtggc tataccggtc acacggccag aggcaatagc 840

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga 900

841 cccttcttca ccgactagag tcggtggcgc ttttactgta gtttttgcgg taattggact 900

.......... .......... ccdB .......... .......... ..........

.......... ..... NotI(924),EagI(924) XhoI(932) ..... ..........

901 tgttctgggg aatatagaat tcgcggccgc actcgagata tctagaccca gctttcttgt 960

901 acaagacccc ttatatctta agcgccggcg tgagctctat agatctgggt cgaaagaaca 960

ccdB .......... .......... .......... .......... attL2

.......... .......... .......... .......... .......... ..........

961 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta 1020

961 tgtttcaacc gtaatattct ttcgtaacga atagttaaac aacgttgctt gtccagtgat 1020

.......... .......... attL2 .......... .......... ..........

.......... .......... .... PvuII(1053),PstI(1058) .... ..........

1021 tcagtcaaaa taaaatcatt atttgccatc cagctgcagc tctggcccgt gtctcaaaat 1080

1021 agtcagtttt attttagtaa taaacggtag gtcgacgtcg agaccgggca cagagtttta 1080

.......... attL2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct 1140

1081 gagactacaa tgtaacgtgt tctattttta tatagtagta cttgttattt tgacagacga 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg 1200

1141 atgtatttgt cattatgttc cccacaatac tcggtataag ttgccctttg cagctccggc 1200

.......... .......... .......... ..... ORF 1 KanR2(variant) .....

.......... .......... .......... .......... NruI(1250) ..........

1201 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc 1260

1201 gctaatttaa ggttgtacct acgactaaat atacccatat ttacccgagc gctattacag 1260

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt 1320

1261 cccgttagtc cacgctgtta gatagcgaac atacccttcg ggctacgcgg tctcaacaaa 1320

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac 1380

1321 gactttgtac cgtttccatc gcaacggtta ctacaatgtc tactctacca gtctgatttg 1380

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat 1440

1381 accgactgcc ttaaatacgg agaaggctgg tagttcgtaa aataggcatg aggactacta 1440

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat 1500

1441 cgtaccaatg agtggtgacg ctaggggcct ttttgtcgta aggtccataa tcttcttata 1500

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt ccctgcgccg gttgcattcg 1560

1501 ggactaagtc cacttttata acaactacgc gaccgtcaca gggacgcggc caacgtaagc 1560

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa 1620

1561 taaggacaaa cattaacagg aaaattgtcg ctagcgcata aagcagagcg agtccgcgtt 1620

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg 1680

1621 agtgcttact tattgccaaa ccaactacgc tcactaaaac tactgctcgc attaccgacc 1680

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc 1740

1681 ggacaacttg ttcagacctt tctttacgta tttgaaaacg gtaagagtgg cctaagtcag 1740

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... AseI(1792)

1741 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt 1800

1741 cagtgagtac cactaaagag tgaactattg gaataaaaac tgctcccctt taattatcca 1800

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg 1860

1801 acataactac aacctgctca gccttagcgt ctggctatgg tcctagaacg gtaggatacc 1860

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt 1920

1861 ttgacggagc cactcaaaag aggaagtaat gtctttgccg aaaaagtttt tataccataa 1920

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca 1980

1921 ctattaggac tatacttatt taacgtcaaa gtaaactacg agctactcaa aaagattagt 1980

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gaattggtta attggttgta acattattca gattgggccc cgttccactg agcgtcagac 2040

1981 cttaaccaat taaccaacat tgtaataagt ctaacccggg gcaaggtgac tcgcagtctg 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccggtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 2100

2041 ggccatcttt tctagtttcc tagaagaact ctaggaaaaa aagacgcgca ttagacgacg 2100

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 2160

2101 aacgtttgtt tttttggtgg cgatggtcgc caccaaacaa acggcctagt tctcgatggt 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 2220

2161 tgagaaaaag gcttccattg accgaagtcg tctcgcgtct atggtttatg acaagaagat 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 2280

2221 cacatcggca tcaatccggt ggtgaagttc ttgagacatc gtggcggatg tatggagcga 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 2340

2281 gacgattagg acaatggtca ccgacgacgg tcaccgctat tcagcacaga atggcccaac 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 2400

2341 ctgagttctg ctatcaatgg cctattccgc gtcgccagcc cgacttgccc cccaagcacg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 2460

2401 tgtgtcgggt cgaacctcgc ttgctggatg tggcttgact ctatggatgt cgcactcgat 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 2520

2461 actctttcgc ggtgcgaagg gcttccctct ttccgcctgt ccataggcca ttcgccgtcc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 2580

2521 cagccttgtc ctctcgcgtg ctccctcgaa ggtccccctt tgcggaccat agaaatatca 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2640

2581 ggacagccca aagcggtgga gactgaactc gcagctaaaa acactacgag cagtcccccc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 2700

2641 gcctcggata cctttttgcg gtcgttgcgc cggaaaaatg ccaaggaccg gaaaacgacc 2700

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccttttgctc acatgtt... .......... .......... .......... .......... 2717

2701 ggaaaacgag tgtacaa... .......... .......... .......... .......... 2717

.......... .......... .......... .......... .......... ..........