Sequence of pENTR2B

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Sequence of pENTR2B

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Sequence of pENTR2B : 2718 BP; 706 A; 605 C; 674 G; 733 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcgatcgt acctagagcc 60

.......... .......... .......... .......... .......... ..........

AatII(66) .......... .......... .......... .......... ..........

61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 120

61 cctgcagatt gatgattcgc tctcatccct tgacggtccg tagtttattt tgctttccga 120

.......... .......... .......... .......... ......... rrnB_T1_term

.......... .......... .......... .......... .......... ..........

121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 180

121 gtcagccttc tgacccggaa agcaaaatag acaacaaaca gccacttgcg agaggactca 180

......... rrnB_T1_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 240

181 tcctgtttag gcggccctcg cctaaacttg caacacttcg ttgccgggcc tcccaccgcc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 300

241 cgtcctgcgg gcggtatttg acggtccgta gtttgattcg tcttccggta ggactgccta 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 360

301 ccggaaaaac gcaaagatgt ttgagaagga caatcaatca atgaattcga gcccggggtt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 420

361 tattactaaa ataaaactga ctatcactgg acaagcaacg ttgtttaact attcgttacg 420

.......... ......... attL2 attL1 ......... .......... ..........

.......... .......... .......... NarI(459) .......... ..........

421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga 480

421 aaaaaatatt acggttgaaa catgtttttt cgtccgaccg cggccttggt taagtcagct 480

......... attL2 attL1 ......... .......... .......... ..........

BamHI(483) KpnI(493) .......... .......... .......... ..........

481 ctggatccgg taccgaattc gcttactaaa agccagataa cagtatgcgt atttgcgcgc 540

481 gacctaggcc atggcttaag cgaatgattt tcggtctatt gtcatacgca taaacgcgcg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg 600

541 actaaaaacg ccatattctt atatatgact atacatatgg gcttcataca gtttttctcc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgtgcttcta gaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg 660

601 acacgaagat cttacgtcaa attccaaatg tggatatttt ctctctcggc aatagcagac 660

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... SmaI(696) .......... ..........

661 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg 720

661 aaacacctac atgtctcact ataataactg tgcgggcccg ctgcctacca ctagggggac 720

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc 780

721 cggtcacgtg cagacgacag tctatttcag agggcacttg aaatgggcca ccacgtatag 780

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc 840

781 cccctacttt cgaccgcgta ctactggtgg ctataccggt cacacggcca gaggcaatag 840

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg 900

841 ccccttcttc accgactaga gtcggtggcg cttttactgt agtttttgcg gtaattggac 900

.......... .......... ccdB .......... .......... ..........

.......... ..... NotI(925),EagI(925) XhoI(933) ..... ..........

901 atgttctggg gaatatagaa ttcgcggccg cactcgagat atctagaccc agctttcttg 960

901 tacaagaccc cttatatctt aagcgccggc gtgagctcta tagatctggg tcgaaagaac 960

ccdB .......... .......... .......... .......... attL2

.......... .......... .......... .......... .......... ..........

961 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact 1020

961 atgtttcaac cgtaatattc tttcgtaacg aatagttaaa caacgttgct tgtccagtga 1020

.......... .......... attL2 .......... .......... ..........

.......... .......... .... PvuII(1054),PstI(1059) .... ..........

1021 atcagtcaaa ataaaatcat tatttgccat ccagctgcag ctctggcccg tgtctcaaaa 1080

1021 tagtcagttt tattttagta ataaacggta ggtcgacgtc gagaccgggc acagagtttt 1080

.......... attL2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 1140

1081 agagactaca atgtaacgtg ttctattttt atatagtagt acttgttatt ttgacagacg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc 1200

1141 aatgtatttg tcattatgtt ccccacaata ctcggtataa gttgcccttt gcagctccgg 1200

.......... .......... .......... ......... ORF 2 KanR2 .........

.......... .......... .......... .......... .......... NruI(1251)

1201 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt 1260

1201 cgctaattta aggttgtacc tacgactaaa tatacccata tttacccgag cgctattaca 1260

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt 1320

1261 gcccgttagt ccacgctgtt agatagcgaa catacccttc gggctacgcg gtctcaacaa 1320

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa 1380

1321 agactttgta ccgtttccat cgcaacggtt actacaatgt ctactctacc agtctgattt 1380

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga 1440

1381 gaccgactgc cttaaatacg gagaaggctg gtagttcgta aaataggcat gaggactact 1440

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata 1500

1441 acgtaccaat gagtggtgac gctaggggcc tttttgtcgt aaggtccata atcttcttat 1500

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc 1560

1501 aggactaagt ccacttttat aacaactacg cgaccgtcac aaggacgcgg ccaacgtaag 1560

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca 1620

1561 ctaaggacaa acattaacag gaaaattgtc gctagcgcat aaagcagagc gagtccgcgt 1620

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg 1680

1621 tagtgcttac ttattgccaa accaactacg ctcactaaaa ctactgctcg cattaccgac 1680

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt 1740

1681 cggacaactt gttcagacct ttctttacgt atttgaaaac ggtaagagtg gcctaagtca 1740

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... AseI(1793)

1741 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg 1800

1741 gcagtgagta ccactaaaga gtgaactatt ggaataaaaa ctgctcccct ttaattatcc 1800

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg 1860

1801 aacataacta caacctgctc agccttagcg tctggctatg gtcctagaac ggtaggatac 1860

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat 1920

1861 cttgacggag ccactcaaaa gaggaagtaa tgtctttgcc gaaaaagttt ttataccata 1920

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc 1980

1921 actattagga ctatacttat ttaacgtcaa agtaaactac gagctactca aaaagattag 1980

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agaattggtt aattggttgt aacattattc agattgggcc ccgttccact gagcgtcaga 2040

1981 tcttaaccaa ttaaccaaca ttgtaataag tctaacccgg ggcaaggtga ctcgcagtct 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 2100

2041 ggggcatctt ttctagtttc ctagaagaac tctaggaaaa aaagacgcgc attagacgac 2100

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2101 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2160

2101 gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca aacggcctag ttctcgatgg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 2220

2161 ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc tatggtttat gacaagaaga 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2280

2221 tcacatcggc atcaatccgg tggtgaagtt cttgagacat cgtggcggat gtatggagcg 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 2340

2281 agacgattag gacaatggtc accgacgacg gtcaccgcta ttcagcacag aatggcccaa 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 2400

2341 cctgagttct gctatcaatg gcctattccg cgtcgccagc ccgacttgcc ccccaagcac 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 2460

2401 gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac tctatggatg tcgcactcga 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 2520

2461 tactctttcg cggtgcgaag ggcttccctc tttccgcctg tccataggcc attcgccgtc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 2580

2521 ccagccttgt cctctcgcgt gctccctcga aggtccccct ttgcggacca tagaaatatc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 2640

2581 aggacagccc aaagcggtgg agactgaact cgcagctaaa aacactacga gcagtccccc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2700

2641 cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat gccaaggacc ggaaaacgac 2700

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gccttttgct cacatgtt.. .......... .......... .......... .......... 2718

2701 cggaaaacga gtgtacaa.. .......... .......... .......... .......... 2718

.......... .......... .......... .......... .......... ..........