Sequence of pENTR3C

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pENTR3C

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pENTR3C : 2723 BP; 710 A; 604 C; 672 G; 737 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcgatcgt acctagagcc 60

.......... .......... .......... .......... .......... ..........

AatII(66) .......... .......... .......... .......... ..........

61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 120

61 cctgcagatt gatgattcgc tctcatccct tgacggtccg tagtttattt tgctttccga 120

.......... .......... .......... .......... ......... rrnB_T1_term

.......... .......... .......... .......... .......... ..........

121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 180

121 gtcagccttc tgacccggaa agcaaaatag acaacaaaca gccacttgcg agaggactca 180

......... rrnB_T1_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 240

181 tcctgtttag gcggccctcg cctaaacttg caacacttcg ttgccgggcc tcccaccgcc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 300

241 cgtcctgcgg gcggtatttg acggtccgta gtttgattcg tcttccggta ggactgccta 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 360

301 ccggaaaaac gcaaagatgt ttgagaagga caatcaatca atgaattcga gcccggggtt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 420

361 tattactaaa ataaaactga ctatcactgg acaagcaacg ttgtttaact attcgttacg 420

.......... ......... attL2 attL1 ......... .......... ..........

.......... .......... .......... .......... DraI(461) ..........

421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg 480

421 aaaaaatatt acggttgaaa catgtttttt cgtccgagaa atttccttgg ttaagtcagc 480

......... attL2 attL1 ......... .......... .......... ..........

BamHI(484) KpnI(494) .......... .......... .......... ..........

481 actggatccg gtaccgaatt cgatcgctta ctaaaagcca gataacagta tgcgtatttg 540

481 tgacctaggc catggcttaa gctagcgaat gattttcggt ctattgtcat acgcataaac 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa 600

541 gcgcgactaa aaacgccata ttcttatata tgactataca tatgggcttc atacagtttt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 agaggtgtgc ttctagaatg cagtttaagg tttacaccta taaaagagag agccgttatc 660

601 tctccacacg aagatcttac gtcaaattcc aaatgtggat attttctctc tcggcaatag 660

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... .......... SmaI(701) ..........

661 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc 720

661 cagacaaaca cctacatgtc tcactataat aactgtgcgg gcccgctgcc taccactagg 720

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc 780

721 gggaccggtc acgtgcagac gacagtctat ttcagagggc acttgaaatg ggccaccacg 780

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg 840

781 tatagcccct actttcgacc gcgtactact ggtggctata ccggtcacac ggccagaggc 840

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta 900

841 aatagcccct tcttcaccga ctagagtcgg tggcgctttt actgtagttt ttgcggtaat 900

.......... .......... ccdB .......... .......... ..........

.......... ..... NotI(930),EagI(930) XhoI(938) ..... ..........

901 acctgatgtt ctggggaata tagaattcgc ggccgcactc gagatatcta gacccagctt 960

901 tggactacaa gaccccttat atcttaagcg ccggcgtgag ctctatagat ctgggtcgaa 960

ccdB .......... .......... .......... .......... attL2

.......... .......... .......... .......... .......... ..........

961 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg 1020

961 agaacatgtt tcaaccgtaa tattctttcg taacgaatag ttaaacaacg ttgcttgtcc 1020

.......... .......... attL2 .......... .......... ..........

.......... .......... .......... .... PvuII(1059),PstI(1064) ....

1021 tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct 1080

1021 agtgatagtc agttttattt tagtaataaa cggtaggtcg acgtcgagac cgggcacaga 1080

.......... attL2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg 1140

1081 gttttagaga ctacaatgta acgtgttcta tttttatata gtagtacttg ttattttgac 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg 1200

1141 agacgaatgt atttgtcatt atgttcccca caatactcgg tataagttgc cctttgcagc 1200

.......... .......... .......... ......... ORF 1 KanR2 .........

.......... .......... .......... .......... .......... NruI(1256)

1201 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 1260

1201 tccggcgcta atttaaggtt gtacctacga ctaaatatac ccatatttac ccgagcgcta 1260

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag 1320

1261 ttacagcccg ttagtccacg ctgttagata gcgaacatac ccttcgggct acgcggtctc 1320

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 1380

1321 aacaaagact ttgtaccgtt tccatcgcaa cggttactac aatgtctact ctaccagtct 1380

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 1440

1381 gatttgaccg actgccttaa atacggagaa ggctggtagt tcgtaaaata ggcatgagga 1440

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa 1500

1441 ctactacgta ccaatgagtg gtgacgctag gggccttttt gtcgtaaggt ccataatctt 1500

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 1560

1501 cttataggac taagtccact tttataacaa ctacgcgacc gtcacaagga cgcggccaac 1560

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 1620

1561 gtaagctaag gacaaacatt aacaggaaaa ttgtcgctag cgcataaagc agagcgagtc 1620

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 1680

1621 cgcgttagtg cttacttatt gccaaaccaa ctacgctcac taaaactact gctcgcatta 1680

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat 1740

1681 ccgaccggac aacttgttca gacctttctt tacgtatttg aaaacggtaa gagtggccta 1740

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... AseI(1798)

1741 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 1800

1741 agtcagcagt gagtaccact aaagagtgaa ctattggaat aaaaactgct cccctttaat 1800

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 1860

1801 tatccaacat aactacaacc tgctcagcct tagcgtctgg ctatggtcct agaacggtag 1860

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 1920

1861 gataccttga cggagccact caaaagagga agtaatgtct ttgccgaaaa agtttttata 1920

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 1980

1921 ccataactat taggactata cttatttaac gtcaaagtaa actacgagct actcaaaaag 1980

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 taatcagaat tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg 2040

1981 attagtctta accaattaac caacattgta ataagtctaa cccggggcaa ggtgactcgc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 2100

2041 agtctggggc atcttttcta gtttcctaga agaactctag gaaaaaaaga cgcgcattag 2100

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 2160

2101 acgacgaacg tttgtttttt tggtggcgat ggtcgccacc aaacaaacgg cctagttctc 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 2220

2161 gatggttgag aaaaaggctt ccattgaccg aagtcgtctc gcgtctatgg tttatgacaa 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 2280

2221 gaagatcaca tcggcatcaa tccggtggtg aagttcttga gacatcgtgg cggatgtatg 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 2340

2281 gagcgagacg attaggacaa tggtcaccga cgacggtcac cgctattcag cacagaatgg 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 2400

2341 cccaacctga gttctgctat caatggccta ttccgcgtcg ccagcccgac ttgcccccca 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 2460

2401 agcacgtgtg tcgggtcgaa cctcgcttgc tggatgtggc ttgactctat ggatgtcgca 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 2520

2461 ctcgatactc tttcgcggtg cgaagggctt ccctctttcc gcctgtccat aggccattcg 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 2580

2521 ccgtcccagc cttgtcctct cgcgtgctcc ctcgaaggtc cccctttgcg gaccatagaa 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 2640

2581 atatcaggac agcccaaagc ggtggagact gaactcgcag ctaaaaacac tacgagcagt 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 2700

2641 ccccccgcct cggatacctt tttgcggtcg ttgcgccgga aaaatgccaa ggaccggaaa 2700

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgctggcctt ttgctcacat gtt....... .......... .......... .......... 2723

2701 acgaccggaa aacgagtgta caa....... .......... .......... .......... 2723

.......... .......... .......... .......... .......... ..........