Sequence of pENTR4

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Sequence of pENTR4

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Sequence of pENTR4 : 2720 BP; 707 A; 606 C; 673 G; 734 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcgatcgt acctagagcc 60

.......... .......... .......... .......... .......... ..........

AatII(66) .......... .......... .......... .......... ..........

61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 120

61 cctgcagatt gatgattcgc tctcatccct tgacggtccg tagtttattt tgctttccga 120

.......... .......... .......... .......... ......... rrnB_T1_term

.......... .......... .......... .......... .......... ..........

121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 180

121 gtcagccttc tgacccggaa agcaaaatag acaacaaaca gccacttgcg agaggactca 180

......... rrnB_T1_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 240

181 tcctgtttag gcggccctcg cctaaacttg caacacttcg ttgccgggcc tcccaccgcc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 300

241 cgtcctgcgg gcggtatttg acggtccgta gtttgattcg tcttccggta ggactgccta 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 360

301 ccggaaaaac gcaaagatgt ttgagaagga caatcaatca atgaattcga gcccggggtt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 420

361 tattactaaa ataaaactga ctatcactgg acaagcaacg ttgtttaact attcgttacg 420

.......... ......... attL2 attL1 ......... .......... ..........

.......... .......... .......... .......... NcoI(461) ..........

421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc 480

421 aaaaaatatt acggttgaaa catgtttttt cgtccgaggt ggtacccttg gttaagtcag 480

......... attL2 attL1 ......... .......... .......... ..........

BamHI(485) KpnI(495) .......... .......... .......... ..........

481 gactggatcc ggtaccgaat tcgcttacta aaagccagat aacagtatgc gtatttgcgc 540

481 ctgacctagg ccatggctta agcgaatgat tttcggtcta ttgtcatacg cataaacgcg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga 600

541 cgactaaaaa cgccatattc ttatatatga ctatacatat gggcttcata cagtttttct 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggtgtgcttc tagaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc 660

601 ccacacgaag atcttacgtc aaattccaaa tgtggatatt ttctctctcg gcaatagcag 660

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... SmaI(698) .......... ..........

661 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc 720

661 acaaacacct acatgtctca ctataataac tgtgcgggcc cgctgcctac cactaggggg 720

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata 780

721 accggtcacg tgcagacgac agtctatttc agagggcact tgaaatgggc caccacgtat 780

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta 840

781 agcccctact ttcgaccgcg tactactggt ggctataccg gtcacacggc cagaggcaat 840

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc 900

841 agccccttct tcaccgacta gagtcggtgg cgcttttact gtagtttttg cggtaattgg 900

.......... .......... ccdB .......... .......... ..........

.......... ..... NotI(927),EagI(927) XhoI(935) ..... ..........

901 tgatgttctg gggaatatag aattcgcggc cgcactcgag atatctagac ccagctttct 960

901 actacaagac cccttatatc ttaagcgccg gcgtgagctc tatagatctg ggtcgaaaga 960

ccdB .......... .......... .......... .......... attL2

.......... .......... .......... .......... .......... ..........

961 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca 1020

961 acatgtttca accgtaatat tctttcgtaa cgaatagtta aacaacgttg cttgtccagt 1020

.......... .......... attL2 .......... .......... ..........

.......... .......... .......... .... PvuII(1056),PstI(1061) ....

1021 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa 1080

1021 gatagtcagt tttattttag taataaacgg taggtcgacg tcgagaccgg gcacagagtt 1080

.......... attL2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 1140

1081 ttagagacta caatgtaacg tgttctattt ttatatagta gtacttgtta ttttgacaga 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg 1200

1141 cgaatgtatt tgtcattatg ttccccacaa tactcggtat aagttgccct ttgcagctcc 1200

.......... .......... .......... ..... ORF 1 KanR2(variant) .....

.......... .......... .......... .......... .......... NruI(1253)

1201 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 1260

1201 ggcgctaatt taaggttgta cctacgacta aatataccca tatttacccg agcgctatta 1260

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg 1320

1261 cagcccgtta gtccacgctg ttagatagcg aacataccct tcgggctacg cggtctcaac 1320

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta 1380

1321 aaagactttg taccgtttcc atcgcaacgg ttactacaat gtctactcta ccagtctgat 1380

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctggt 1440

1381 ttgaccgact gccttaaata cggagaaggc tggtagttcg taaaataggc atgaggacca 1440

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa 1500

1441 ctacgtacca atgagtggtg acgctagggg cctttttgtc gtaaggtcca taatcttctt 1500

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 1560

1501 ataggactaa gtccactttt ataacaacta cgcgaccgtc acaaggacgc ggccaacgta 1560

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg 1620

1561 agctaaggac aaacattaac aggaaaattg tcgctagcgc ataaagcaga gcgagtccgc 1620

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc 1680

1621 gttagtgctt acttattgcc aaaccaacta cgctcactaa aactactgct cgcattaccg 1680

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca 1740

1681 accggacaac ttgttcagac ctttctttac gtatttgaaa acggtaagag tggcctaagt 1740

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... AseI(1795)

1741 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 1800

1741 cagcagtgag taccactaaa gagtgaacta ttggaataaa aactgctccc ctttaattat 1800

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 1860

1801 ccaacataac tacaacctgc tcagccttag cgtctggcta tggtcctaga acggtaggat 1860

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 1920

1861 accttgacgg agccactcaa aagaggaagt aatgtctttg ccgaaaaagt ttttatacca 1920

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa 1980

1921 taactattag gactatactt atttaacgtc aaagtaaact acgagctact caaaaagatt 1980

.......... ..... ORF 1 KanR2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcagaattgg ttaattggtt gtaacattat tcagattggg ccccgttcca ctgagcgtca 2040

1981 agtcttaacc aattaaccaa cattgtaata agtctaaccc ggggcaaggt gactcgcagt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 2100

2041 ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg 2100

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2101 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 2160

2101 acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 2220

2161 ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 2280

2221 gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 2340

2281 cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 2400

2341 aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 2460

2401 acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 2520

2461 gatactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 2580

2521 tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 2640

2581 tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 2700

2641 cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg 2700

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tggccttttg ctcacatgtt .......... .......... .......... .......... 2720

2701 accggaaaac gagtgtacaa .......... .......... .......... .......... 2720

.......... .......... .......... .......... .......... ..........