Sequence of pENTR

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Sequence of pENTR_D-TOPO

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Sequence of pENTR/D-TOPO : 2580 BP; 647 A; 622 C; 635 G; 676 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 120

61 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 180

121 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 240

181 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattat gcgcatggcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 300

241 atcggtcctt ctcaaacatc tttgcgtttt tccggtaggc agtcctaccg gaagacgaat 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 360

301 caaactacgg accgtcaaat accgcccgca ggacgggcgg tgggaggccc ggcaacgaag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 420

361 tgttgcaagt ttaggcgagg gccgcctaaa caggatgagt cctctcgcaa gtggctgttt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 480

421 gttgtctatt ttgctttccg ggtcagaagg ctgactcgga aagcaaaata aactacggac 480

.......... ......... rrnB_T1_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 540

481 cgtcaaggga tgagagcgca attgcgatcg tacctacaaa agggtcagtg ctgcaacatt 540

.......... .......... .......... .......... ....... M13_pUC_fwd_pri

.......... AflII(554) ApaI(567) .......... .......... ..........

541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 600

541 ttgctgccgg tcagaattcg agcccggggt ttattactaa aataaaactg actatcactg 600

M13_forward20_pri .. .......... .......... attL1 ..........

.......... .......... .......... .......... .......... ..........

601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 660

601 gacaagcaac gttgtttaac tactcgttac gaaaaaatat tacggttgaa acatgttttt 660

.......... .......... attL1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agcaggctcc gcggccgccc ccttcaccaa gggtgggcgc gccgacccag ctttcttgta 720

661 tcgtccgagg cgccggcggg ggaagtggtt cccacccgcg cggctgggtc gaaagaacat 720

.......... .......... .......... .......... .......... attL2

.......... .......... .......... .......... .......... ..........

721 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat 780

721 gtttcaaccg taatattctt tcgtaacgaa tagttaaaca acgttgcttg tccagtgata 780

.......... .......... attL2 .......... .......... ..........

.......... .......... .......... EcoRV(817) .......... ..........

781 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta 840

781 gtcagtttta ttttagtaat aaacggtagg tcgactatag gggatatcac tcagcataat 840

.......... attL2 .......... .......... .......... T7_pro

.......... .......... .......... .......... .......... ..........

841 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat 900

841 gtaccagtat cgacaaagga ccgtcgagac cgggcacaga gttttagaga ctacaatgta 900

M13_reverse_pri .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa 960

901 acgtgttcta tttttatata gtagtacttg ttattttgac agacgaatgt atttgtcatt 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa 1020

961 atgttcccca caatactcgg tataagttgc cctttgcagc tccggcgcta atttaaggtt 1020

.......... .......... ......... ORF 2 KanR2 ......... ..........

.......... .......... .......... NruI(1056) .......... ..........

1021 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc 1080

1021 gtacctacga ctaaatatac ccatatttac ccgagcgcta ttacagcccg ttagtccacg 1080

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa 1140

1081 ctgttagata gcgaacatac ccttcgggct acgcggtctc aacaaagact ttgtaccgtt 1140

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt 1200

1141 tccatcgcaa cggttactac aatgtctact ctaccagtct gatttgaccg actgccttaa 1200

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac 1260

1201 atacggagaa ggctggtagt tcgtaaaata ggcatgagga ctactacgta ccaatgagtg 1260

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga 1320

1261 gtgacgctag gggccttttt gtcgtaaggt ccataatctt cttataggac taagtccact 1320

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa 1380

1321 tttataacaa ctacgcgacc gtcacaagga cgcggccaac gtaagctaag gacaaacatt 1380

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa 1440

1381 aacaggaaaa ttgtcgctag cgcataaagc agagcgagtc cgcgttagtg cttacttatt 1440

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt 1500

1441 gccaaaccaa ctacgctcac taaaactact gctcgcatta ccgaccggac aacttgttca 1500

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga 1560

1501 gacctttctt tacgtatttg aaaacggtaa gagtggccta agtcagcagt gagtaccact 1560

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg 1620

1561 aaagagtgaa ctattggaat aaaaactgct cccctttaat tatccaacat aactacaacc 1620

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga 1680

1621 tgctcagcct tagcgtctgg ctatggtcct agaacggtag gataccttga cggagccact 1680

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat 1740

1681 caaaagagga agtaatgtct ttgccgaaaa agtttttata ccataactat taggactata 1740

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg 1800

1741 cttatttaac gtcaaagtaa actacgagct actcaaaaag attagtctta accaattaac 1800

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa 1860

1801 caacattgtg accgtctcgt aatgcgactg aactgccctg ccgcgttcga gtactggttt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1920

1861 tagggaattg cactcaatgc gcagcaaggt gactcgcagt ctggggcatc ttttctagtt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1980

1921 tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt gtttttttgg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 2040

1981 tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa aaggcttcca 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 2100

2041 ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa gatcacatcg gcatcaatcc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 2160

2101 ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt aggacaatgg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 2220

2161 tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt ctgctatcaa 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... ......... ApaLI(2260) ......... ..........

2221 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 2280

2221 tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg ggtcgaacct 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct 2340

2281 cgcttgctgg atgtggcttg actctatgga tgtcgcactc gtaactcttt cgcggtgcga 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2400

2341 agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt gtcctctcgc 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2460

2401 gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc ccaaagcggt 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2520

2461 ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg ataccttttt 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2580

2521 gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac gagtgtacaa 2580

pBR322_origin ...... .......... .......... .......... ..........