Sequence of pENTR_H1

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Sequence of pENTR_H1_TO

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Sequence of pENTR/H1/TO : 3869 BP; 956 A; 963 C; 977 G; 973 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 120

61 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 180

121 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 240

181 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattat gcgcatggcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 300

241 atcggtcctt ctcaaacatc tttgcgtttt tccggtaggc agtcctaccg gaagacgaat 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 360

301 caaactacgg accgtcaaat accgcccgca ggacgggcgg tgggaggccc ggcaacgaag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 420

361 tgttgcaagt ttaggcgagg gccgcctaaa caggatgagt cctctcgcaa gtggctgttt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 480

421 gttgtctatt ttgctttccg ggtcagaagg ctgactcgga aagcaaaata aactacggac 480

.......... ......... rrnB_T1_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagttccct actctcgcgt taattcgccc ttagacatga taagatacat tgatgagttt 540

481 cgtcaaggga tgagagcgca attaagcggg aatctgtact attctatgta actactcaaa 540

.......... .......... .......... ........ SV40_PA_term EBV_rev_pri

.......... .......... .......... .......... .......... ..........

541 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct 600

541 cctgtttggt gttgatctta cgtcactttt tttacgaaat aaacacttta aacactacga 600

EBV_rev_pri ....... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 attgctttat ttgtaaccat tataagctgc aataaacaag ttggggtggg cgaagaactc 660

601 taacgaaata aacattggta atattcgacg ttatttgttc aaccccaccc gcttcttgag 660

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa 720

661 gtcgtactct aggggcgcga cctcctagta ggtcggccgc agggcctttt gctaaggctt 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtagcacgt gtcagtcctg 780

721 cgggttggaa agtatcttcc gccgccacct tagctttaga gcatcgtgca cagtcaggac 780

.......... .......... .......... .......... .......... sh_ble

.......... .......... EagI(810) .......... .......... ..........

781 ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga actcccgccc 840

781 gaggagccgg tgcttcacgt gcgtcaacgg ccggcccagc gcgtcccgct tgagggcggg 840

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga agttcgtgga 900

841 ggtgccgacg agcggctaga gccagtaccg gccgggcctc cgcagggcct tcaagcacct 900

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca cccaggccag 960

901 gtgctggagg ctggtgagcc gcatgtcgag caggtccggc gcgtgggtgt gggtccggtc 960

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggtgttgtcc ggcaccatct ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg 1020

961 ccacaacagg ccgtggtaga ccaggacctg gcgcgactac ttgtcccagt gcagcagggc 1020

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc ggtcggtcca 1080

1021 ctggtgtggc cgcttcagca ggaggtgctt cagggccctc ttgggctcgg ccagccaggt 1080

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt 1140

1081 cttgagctgg cgaggccgct gcagcgcgcg ccactcgtgg ccttgccgtg accagttgaa 1140

.......... ......... sh_ble bleo ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggccatggtt tagttcctca ccttgtcgta ttatactatg ccgatatact atgccgatga 1200

1141 ccggtaccaa atcaaggagt ggaacagcat aatatgatac ggctatatga tacggctact 1200

.......... .......... .......... EM7_pro .......... ..........

.......... BclI(1219) .......... .......... .......... ..........

1201 ttaattgtca acacgtgctg atcagatccg aaaatggata tacaagctcc cgggagcttt 1260

1201 aattaacagt tgtgcacgac tagtctaggc ttttacctat atgttcgagg gccctcgaaa 1260

EM7_pro .......... .......... .......... .......... ..........

.......... StuI(1275) .......... .......... .......... ..........

1261 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggca 1320

1261 aacgttttcg gatccggagg ttttttcgga ggagtgatga agaccttatc gagtctccgt 1320

.......... .......... .......... .......... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

1321 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg 1380

1321 ctccgccgga gccggagacg tatttatttt ttttaatcag tcggtacccc gcctcttacc 1380

....... SV40pro_F_pri SV40_pro SV40_origin ....... ..........

.......... .......... .......... .......... .......... ..........

1381 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct 1440

1381 cgccttgacc cgcctcaatc cccgccctac ccgcctcaat ccccgccctg ataccaacga 1440

..... SV40_origin SV40_pro SV40_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca 1500

1441 ctgattaact ctacgtacga aacgtatgaa gacggacgac ccctcggacc cctgaaaggt 1500

.......... ...... SV40_pro SV40_enh ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct 1560

1501 gtggaccaac gactgattaa ctctacgtac gaaacgtatg aagacggacg acccctcgga 1560

.......... ...... SV40_pro SV40_enh ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggggactttc cacaccctaa ctgacacaca ttccacagcc gaagggcgaa ttaacgctag 1620

1561 cccctgaaag gtgtgggatt gactgtgtgt aaggtgtcgg cttcccgctt aattgcgatc 1620

SV40_pro SV40_enh pBABE_3_pri ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc 1680

1621 gtacctacaa aagggtcagt gctgcaacat tttgctgccg gtcagaattc gagcccgggg 1680

.......... ... M13_pUC_fwd_pri M13_forward20_pri ... ..........

.......... .......... .......... .......... .......... ..........

1681 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat 1740

1681 tttattacta aaataaaact gactatcact ggacaagcaa cgttgtttaa ctactcgtta 1740

.......... .......... attL1 .......... .......... ..........

.......... .......... .......... .......... DraI(1781) ..........

1741 gcttttttat aatgccaact ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg 1800

1741 cgaaaaaata ttacggttga aacatgtttt ttcgtccgaa atttccttgg ttaagtcagc 1800

.......... attL1 .......... .......... .......... ..........

......... KpnI(1814) BglII(1830) ......... .......... ..........

1801 actggatccg gtaccgggcc ccccctcgaa gatctaatat ttgcatgtcg ctatgtgttc 1860

1801 tgacctaggc catggcccgg gggggagctt ctagattata aacgtacagc gatacacaag 1860

.......... .......... .......... .......... .......... H1_pri

.......... .......... .......... .......... .......... ..........

1861 tgggaaatca ccataaacgt gaaatcccta tcagtgatag agacttataa gttccctatc 1920

1861 accctttagt ggtatttgca ctttagggat agtcactatc tctgaatatt caagggatag 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agtgatagag acaccttttt tgtcgagctt ccttcgggaa gctctccata ttttttggat 1980

1921 tcactatctc tgtggaaaaa acagctcgaa ggaagccctt cgagaggtat aaaaaaccta 1980

.......... .......... .......... .......... .......... ..........

SpeI(1983) .......... .......... .......... .......... ..........

1981 ccactagttc tagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta 2040

1981 ggtgatcaag atctgggtcg aaagaacatg tttcaaccgt aatattcttt cgtaacgaat 2040

pBluescriptSK_pri .. .......... attL2 .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca 2100

2041 agttaaacaa cgttgcttgt ccagtgatag tcagttttat tttagtaata aacggtaggt 2100

.......... .......... attL2 .......... .......... ..........

EcoRV(2106) ........ .......... .......... .......... ..........

2101 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg 2160

2101 cgactatagg ggatatcact cagcataatg taccagtatc gacaaaggac cgtcgagacc 2160

.......... T7_pro ....... M13_reverse_pri ....... ..........

.......... .......... .......... .......... .......... ..........

2161 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca 2220

2161 gggcacagag ttttagagac tacaatgtaa cgtgttctat ttttatatag tagtacttgt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg 2280

2221 tattttgaca gacgaatgta tttgtcatta tgttccccac aatactcggt ataagttgcc 2280

.......... .......... .......... .......... ......... ORF 1 KanR2

.......... .......... .......... .......... .......... ..........

2281 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg 2340

2281 ctttgcagct ccggcgctaa tttaaggttg tacctacgac taaatatacc catatttacc 2340

.......... ......... ORF 1 KanR2 ......... .......... ..........

NruI(2345) .......... .......... .......... .......... ..........

2341 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat 2400

2341 cgagcgctat tacagcccgt tagtccacgc tgttagatag cgaacatacc cttcgggcta 2400

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag 2460

2401 cgcggtctca acaaagactt tgtaccgttt ccatcgcaac ggttactaca atgtctactc 2460

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc 2520

2461 taccagtctg atttgaccga ctgccttaaa tacggagaag gctggtagtt cgtaaaatag 2520

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag 2580

2521 gcatgaggac tactacgtac caatgagtgg tgacgctagg ggcctttttg tcgtaaggtc 2580

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg 2640

2581 cataatcttc ttataggact aagtccactt ttataacaac tacgcgaccg tcacaaggac 2640

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt 2700

2641 gcggccaacg taagctaagg acaaacatta acaggaaaat tgtcgctagc gcataaagca 2700

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac 2760

2701 gagcgagtcc gcgttagtgc ttacttattg ccaaaccaac tacgctcact aaaactactg 2760

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc 2820

2761 ctcgcattac cgaccggaca acttgttcag acctttcttt acgtatttga aaacggtaag 2820

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag 2880

2821 agtggcctaa gtcagcagtg agtaccacta aagagtgaac tattggaata aaaactgctc 2880

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat 2940

2881 ccctttaatt atccaacata actacaacct gctcagcctt agcgtctggc tatggtccta 2940

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt 3000

2941 gaacggtagg ataccttgac ggagccactc aaaagaggaa gtaatgtctt tgccgaaaaa 3000

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat 3060

3001 gtttttatac cataactatt aggactatac ttatttaacg tcaaagtaaa ctacgagcta 3060

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact 3120

3061 ctcaaaaaga ttagtcttaa ccaattaacc aacattgtga ccgtctcgta atgcgactga 3120

ORF 1 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac 3180

3121 actgccctgc cgcgttcgag tactggtttt agggaattgc actcaatgcg cagcaaggtg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 3240

3181 actcgcagtc tggggcatct tttctagttt cctagaagaa ctctaggaaa aaaagacgcg 3240

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3241 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 3300

3241 cattagacga cgaacgtttg tttttttggt ggcgatggtc gccaccaaac aaacggccta 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 3360

3301 gttctcgatg gttgagaaaa aggcttccat tgaccgaagt cgtctcgcgt ctatggttta 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 3420

3361 tgacaagaag atcacatcgg catcaatccg gtggtgaagt tcttgagaca tcgtggcgga 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 3480

3421 tgtatggagc gagacgatta ggacaatggt caccgacgac ggtcaccgct attcagcaca 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 3540

3481 gaatggccca acctgagttc tgctatcaat ggcctattcc gcgtcgccag cccgacttgc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 3600

3541 cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga tgtggcttga ctctatggat 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 3660

3601 gtcgcactcg atactctttc gcggtgcgaa gggcttccct ctttccgcct gtccataggc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 3720

3661 cattcgccgt cccagccttg tcctctcgcg tgctccctcg aaggtccccc tttgcggacc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 3780

3721 atagaaatat caggacagcc caaagcggtg gagactgaac tcgcagctaa aaacactacg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 3840

3781 agcagtcccc ccgcctcgga tacctttttg cggtcgttgc gccggaaaaa tgccaaggac 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gccttttgct ggccttttgc tcacatgtt. .......... .......... .......... 3869

3841 cggaaaacga ccggaaaacg agtgtacaa. .......... .......... .......... 3869

.......... .......... .......... .......... .......... ..........