TT TT LOCUS pENTR L1-pLac-LacZalpha-R5 2968 bp DNA SYN 08-Nov-2007 DEFINITION pENTR L1-pLac-LacZalpha-R5 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..2968 /organism="pENTR L1-pLac-LacZalpha-R5" /mol_type="other DNA" terminator complement(268..295) /label="rrnB_T2_terminator" terminator complement(427..470) /label="rrnB_T1_terminator" misc_feature 522..544 /label="M13_pUC_fwd_primer" misc_feature 537..553 /label="M13_forward20_primer" misc_feature complement(569..668) /label="attL1" promoter 694..723 /label="lac_promoter" misc_feature 737..759 /label="M13_pUC_rev_primer" misc_feature 758..776 /label="M13_reverse_primer" misc_feature complement(842..858) /label="M13_forward20_primer" misc_feature complement(851..873) /label="M13_pUC_fwd_primer" misc_feature 839..982 /label="lacZ_a" misc_feature complement(1054..1154) /label="attR2" misc_feature 1054..1154 /label="attR1" promoter complement(1210..1228) /label="T7_promoter" misc_feature complement(1229..1247) /label="M13_reverse_primer" CDS 1362..2171 /label="KanR2" /gene="KanR2" /note="ORF frame 3" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" gene 1362..2171 /label="KanR2" /gene="KanR2" rep_origin 2302..2921 /label="pBR322_origin" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT 721 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA 781 CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCGGGTACCG AGCTCGAATT 841 CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC 901 GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC 961 GCCCTTCCCA ACAGTTGCGC AGCTAACACT ATAAAAAGGC AGTGATGCAG ACGGGGCTTT 1021 TTTATGCAGG CAACTTTGTA TACAAAAGTT GAACGAGAAA CGTAAAATGA TATAAATATC 1081 AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT 1141 ATCCAGTCAC TATGAATCAA CTACTTAGAT GGTATTAGTG ACCTGTACTG CAGGGCGGCC 1201 GCGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC 1261 CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA AAATATATCA TCATGAACAA 1321 TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG 1381 AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG 1441 CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG 1501 CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA 1561 TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC 1621 GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG 1681 TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC 1741 GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC 1801 TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG 1861 AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT 1921 CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG 1981 GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC 2041 TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC 2101 AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG 2161 AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT 2221 GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT 2281 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 2341 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 2401 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 2461 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 2521 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 2581 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 2641 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 2701 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 2761 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 2821 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 2881 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 2941 CCTTTTGCTG GCCTTTTGCT CACATGTT // pENTR L1-pLac-LacZalpha-R5 "Times New RomanTT C ' rrnB_T2_term"Times New RomanTT C  rrnB_T1_term"Times New RomanTT CɆ  M13_pUC_fwd_pri"Times New RomanTT  )M13_forward20_pri"Times New RomanTT C 9attL1"Times New RomanTT C lac_pro"Times New RomanTT CɆ M13_pUC_rev_pri"Times New RomanTT  M13_reverse_pri"Times New RomanTT CɆ GlacZ_a"Times New RomanTT C JZM13_forward20_pri"Times New RomanTT C SiM13_pUC_fwd_pri"Times New Roman)TT  attR2"Times New RomanTT C attR1"Times New RomanTT C T7_pro"Times New RomanTT CɆ M13_reverse_pri"Times New RomanTT  R{ORF 3"Times New RomanTT C R{KanR2"Times New RomanTT C i  pBR322_origin"Times New Roman TT HpaI(501),AflII(554),ApaI(567)"Times New Roman 5FWW TT THindIII(785),SalI(803),XbaI(809),BamHI(815),SmaI(822),KpnI(828),SacI(834),EcoRI(836)"Times New Roman SNgOzPz TT FspI"Times New Roman 9J[[ TT !NotI(1196),EagI(1196),EcoRV(1205)"Times New Roman UC TT NruI"Times New Roman 3FeFU TT ApaLI"Times New RomanX  wg{Xg TT test"Times New RomanQ G<$$ TT test"Times New Roman HGk~F TT test"Times New Roman 33