Sequence of pENTR_L1-pLac-LacZalpha-R5

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Sequence of pENTR_L1-pLac-LacZalpha-R5

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Sequence of pENTR L1-pLac-LacZalpha-R5 : 2968 BP; 765 A; 713 C; 719 G; 771 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 120

61 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 180

121 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 240

181 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattat gcgcatggcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 300

241 atcggtcctt ctcaaacatc tttgcgtttt tccggtaggc agtcctaccg gaagacgaat 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 360

301 caaactacgg accgtcaaat accgcccgca ggacgggcgg tgggaggccc ggcaacgaag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 420

361 tgttgcaagt ttaggcgagg gccgcctaaa caggatgagt cctctcgcaa gtggctgttt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 480

421 gttgtctatt ttgctttccg ggtcagaagg ctgactcgga aagcaaaata aactacggac 480

.......... ......... rrnB_T1_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 540

481 cgtcaaggga tgagagcgca attgcgatcg tacctacaaa agggtcagtg ctgcaacatt 540

.......... .......... .......... .......... ....... M13_pUC_fwd_pri

.......... AflII(554) ApaI(567) .......... .......... ..........

541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 600

541 ttgctgccgg tcagaattcg agcccggggt ttattactaa aataaaactg actatcactg 600

M13_forward20_pri .. .......... .......... attL1 ..........

.......... .......... .......... .......... .......... ..........

601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 660

601 gacaagcaac gttgtttaac tactcgttac gaaaaaatat tacggttgaa acatgttttt 660

.......... .......... attL1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agcaggctag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 720

661 tcgtccgatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag gccgagcata 720

.......... .......... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

721 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta 780

721 caacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac tggtactaat 780

.......... ....... M13_pUC_rev_pri .... M13_reverse_pri .......

HindIII SalI XbaI BamHI SmaI KpnI SacI,EcoRI(836)

781 cgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt 840

781 gcggttcgaa cgtacggacg tccagctgag atctcctagg ggcccatggc tcgagcttaa 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 900

841 gtgaccggca gcaaaatgtt gcagcactga cccttttggg accgcaatgg gttgaattag 900

M13_forward20_pri lacZ_a M13_pUC_fwd_pri .......... ..........

.......... .......... .......... .......... .......... ..........

901 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 960

901 cggaacgtcg tgtaggggga aagcggtcga ccgcattatc gcttctccgg gcgtggctag 960

.......... .......... lacZ_a .......... .......... ..........

.......... FspI(978) .......... .......... .......... ..........

961 gcccttccca acagttgcgc agctaacact ataaaaaggc agtgatgcag acggggcttt 1020

961 cgggaagggt tgtcaacgcg tcgattgtga tatttttccg tcactacgtc tgccccgaaa 1020

.......... lacZ_a .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tttatgcagg caactttgta tacaaaagtt gaacgagaaa cgtaaaatga tataaatatc 1080

1021 aaatacgtcc gttgaaacat atgttttcaa cttgctcttt gcattttact atatttatag 1080

.......... .......... .......... ......... attR2 attR1 .........

.......... .......... .......... .......... .......... ..........

1081 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat 1140

1081 ttatataatt taatctaaaa cgtatttttt gtctgatgta ttatgacatt ttgtgttgta 1140

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 atccagtcac tatgaatcaa ctacttagat ggtattagtg acctgtactg cagggcggcc 1200

1141 taggtcagtg atacttagtt gatgaatcta ccataatcac tggacatgac gtcccgccgg 1200

attR2 attR1 ........ .......... .......... .......... ..........

EcoRV(1205) ........ .......... .......... .......... ..........

1201 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc 1260

1201 cgctataggg gatatcactc agcataatgt accagtatcg acaaaggacc gtcgagaccg 1260

.......... T7_pro ....... M13_reverse_pri ....... ..........

.......... .......... .......... .......... .......... ..........

1261 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa 1320

1261 ggcacagagt tttagagact acaatgtaac gtgttctatt tttatatagt agtacttgtt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg 1380

1321 attttgacag acgaatgtat ttgtcattat gttccccaca atactcggta taagttgccc 1380

.......... .......... .......... .......... ......... ORF 3 KanR2

.......... .......... .......... .......... .......... ..........

1381 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg 1440

1381 tttgcagctc cggcgctaat ttaaggttgt acctacgact aaatataccc atatttaccc 1440

.......... ......... ORF 3 KanR2 ......... .......... ..........

NruI(1444) .......... .......... .......... .......... ..........

1441 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg 1500

1441 gagcgctatt acagcccgtt agtccacgct gttagatagc gaacataccc ttcgggctac 1500

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga 1560

1501 gcggtctcaa caaagacttt gtaccgtttc catcgcaacg gttactacaa tgtctactct 1560

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc 1620

1561 accagtctga tttgaccgac tgccttaaat acggagaagg ctggtagttc gtaaaatagg 1620

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg 1680

1621 catgaggact actacgtacc aatgagtggt gacgctaggg gcctttttgt cgtaaggtcc 1680

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc 1740

1681 ataatcttct tataggacta agtccacttt tataacaact acgcgaccgt cacaaggacg 1740

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc 1800

1741 cggccaacgt aagctaagga caaacattaa caggaaaatt gtcgctagcg cataaagcag 1800

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg 1860

1801 agcgagtccg cgttagtgct tacttattgc caaaccaact acgctcacta aaactactgc 1860

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct 1920

1861 tcgcattacc gaccggacaa cttgttcaga cctttcttta cgtatttgaa aacggtaaga 1920

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg 1980

1921 gtggcctaag tcagcagtga gtaccactaa agagtgaact attggaataa aaactgctcc 1980

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc 2040

1981 cctttaatta tccaacataa ctacaacctg ctcagcctta gcgtctggct atggtcctag 2040

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc 2100

2041 aacggtagga taccttgacg gagccactca aaagaggaag taatgtcttt gccgaaaaag 2100

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg 2160

2101 tttttatacc ataactatta ggactatact tatttaacgt caaagtaaac tacgagctac 2160

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt 2220

2161 tcaaaaagat tagtcttaac caattaacca acattgtgac cgtctcgtaa tgcgactgaa 2220

ORF 3 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact 2280

2221 ctgccctgcc gcgttcgagt actggtttta gggaattgca ctcaatgcgc agcaaggtga 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 2340

2281 ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa aaagacgcgc 2340

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 2400

2341 attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca aacggcctag 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 2460

2401 ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc tatggtttat 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 2520

2461 gacaagaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat cgtggcggat 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 2580

2521 gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta ttcagcacag 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2640

2581 aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc ccgacttgcc 2640

.......... ........ pBR322_origin ........ .......... ..........

ApaLI(2648) ........ .......... .......... .......... ..........

2641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2700

2641 ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac tctatggatg 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2760

2701 tcgcactcga tactctttcg cggtgcgaag ggcttccctc tttccgcctg tccataggcc 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 2820

2761 attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct ttgcggacca 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 2880

2821 tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa aacactacga 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 2940

2881 gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat gccaaggacc 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccttttgctg gccttttgct cacatgtt.. .......... .......... .......... 2968

2941 ggaaaacgac cggaaaacga gtgtacaa.. .......... .......... .......... 2968

.......... .......... .......... .......... .......... ..........