Sequence of pENTR_L5-pLac-Spect-L2

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Sequence of pENTR_L5-pLac-Spect-L2

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Sequence of pENTR L5-pLac-Spect-L2 : 3713 BP; 924 A; 886 C; 955 G; 948 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 120

61 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 180

121 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 240

181 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattat gcgcatggcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 300

241 atcggtcctt ctcaaacatc tttgcgtttt tccggtaggc agtcctaccg gaagacgaat 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 360

301 caaactacgg accgtcaaat accgcccgca ggacgggcgg tgggaggccc ggcaacgaag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 420

361 tgttgcaagt ttaggcgagg gccgcctaaa caggatgagt cctctcgcaa gtggctgttt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 480

421 gttgtctatt ttgctttccg ggtcagaagg ctgactcgga aagcaaaata aactacggac 480

.......... ......... rrnB_T1_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 540

481 cgtcaaggga tgagagcgca attgcgatcg tacctacaaa agggtcagtg ctgcaacatt 540

.......... .......... .......... .......... ....... M13_pUC_fwd_pri

.......... AflII(554) ApaI(567) .......... .......... ..........

541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 600

541 ttgctgccgg tcagaattcg agcccggggt ttattactaa aataaaactg actatcactg 600

M13_forward20_pri .. .......... .......... attL1 ..........

.......... .......... .......... .......... .......... ..........

601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa 660

601 gacaagcaac gttgtttaac tactcgttac gaaaaaatat tacggttgaa acatatgttt 660

.......... .......... attL1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agttggttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 720

661 tcaaccaatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag gccgagcata 720

.......... .......... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

721 attgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg catcgctcac 780

721 taacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac gtagcgagtg 780

.......... ....... M13_pUC_rev_pri ....... .......... ORF 3

.......... .......... .......... .......... .......... ..........

781 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca 840

781 cgttgaccag gtcttggaac tggcttgcgt cgccaccatt gccgcgtcac cgccaaaagt 840

.......... .......... ORF 3 spect .......... ..........

.......... .......... .......... .......... .......... ..........

841 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag 900

841 accgaacaat actgacaaaa aaaccccatg tcagatacgg agcccgtagg ttcgtcgttc 900

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg 960

901 gcgcaatgcg gcacccagct acaaactaca atacctcgtc gttgctacaa tgcgtcgtcc 960

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga 1020

961 cgtcagcggg attttgtttc aatttgtaat actcccttcg ccactagcgg cttcatagct 1020

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg 1080

1021 gagttgatag tctccatcaa ccgcagtagc tcgcggtaga gcttggctgc aacgaccggc 1080

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc 1140

1081 atgtaaacat gccgaggcgt cacctaccgc cggacttcgg tgtgtcacta taactaaacg 1140

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tggttacggt gaccgcaagg cttgatgaaa caacgcggcg agctttgatc gacgaccttt 1200

1141 accaatgcca ctggcgttcc gaactacttt gttgcgccgc tcgaaactag ctgctggaaa 1200

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg 1260

1201 acctttgaag ccgaagggga cctctctcgc tctaagaggc gcgacatctt cagtggtaac 1260

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag 1320

1261 aacacgtgct gctgtagtaa ggcaccgcaa taggtcgatt cgcgcttgac gttaaacctc 1320

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc 1380

1321 ttaccgtcgc gttactgtaa gaacgtccat agaagctcgg tcggtgctag ctgtaactag 1380

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg 1440

1381 accgatagaa cgactgtttt cgttctcttg tatcgcaacg gaaccatcca ggtcgccgcc 1440

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa 1500

1441 tccttgagaa actaggccaa ggacttgtcc tagataaact ccgcgattta ctttggaatt 1500

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt 1560

1501 gcgatacctt gagcggcggg ctgacccgac cgctactcgc tttacatcac gaatgcaaca 1560

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact 1620

1561 gggcgtaaac catgtcgcgt cattggccgt tttagcgcgg cttcctacag cgacggctga 1620

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt 1680

1621 cccgttacct cgcggacggc cgggtcatag tcgggcagta tgaacttcga tctgtccgaa 1680

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc 1740

1681 tagaacctgt tcttcttcta gcgaaccgga gcgcgcgtct agtcaacctt cttaaacagg 1740

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 actacgtgaa aggcgagatc accaaggtag tcggcaaata acactataag ggcggatgat 1800

1741 tgatgcactt tccgctctag tggttccatc agccgtttat tgtgatattc ccgcctacta 1800

.......... ......... ORF 3 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcagacgggg cttttttatg caggacccag ctttcttgta caaagttggc attataagaa 1860

1801 cgtctgcccc gaaaaaatac gtcctgggtc gaaagaacat gtttcaaccg taatattctt 1860

.......... .......... .......... .......... attL2 ..........

.......... .......... .......... .......... .......... ..........

1861 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta 1920

1861 tcgtaacgaa tagttaaaca acgttgcttg tccagtgata gtcagtttta ttttagtaat 1920

.......... .......... attL2 .......... .......... ..........

... PstI(1937),NotI(1941),EagI(1941) EcoRV(1950) ... ..........

1921 tttgccatcc agctgcaggg cggccgcgat atcccctata gtgagtcgta ttacatggtc 1980

1921 aaacggtagg tcgacgtccc gccggcgcta taggggatat cactcagcat aatgtaccag 1980

.......... .......... .......... .......... T7_pro ..........

.......... .......... .......... .......... .......... ..........

1981 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa 2040

1981 tatcgacaaa ggaccgtcga gaccgggcac agagttttag agactacaat gtaacgtgtt 2040

M13_reverse_pri .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg 2100

2041 ctatttttat atagtagtac ttgttatttt gacagacgaa tgtatttgtc attatgttcc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat 2160

2101 ccacaatact cggtataagt tgccctttgc agctccggcg ctaatttaag gttgtaccta 2160

.......... .......... ......... ORF 1 KanR2 ......... ..........

.......... .......... NruI(2189) .......... .......... ..........

2161 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc 2220

2161 cgactaaata tacccatatt tacccgagcg ctattacagc ccgttagtcc acgctgttag 2220

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc 2280

2221 atagcgaaca tacccttcgg gctacgcggt ctcaacaaag actttgtacc gtttccatcg 2280

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct 2340

2281 caacggttac tacaatgtct actctaccag tctgatttga ccgactgcct taaatacgga 2340

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg 2400

2341 gaaggctggt agttcgtaaa ataggcatga ggactactac gtaccaatga gtggtgacgc 2400

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt 2460

2401 taggggcctt tttgtcgtaa ggtccataat cttcttatag gactaagtcc acttttataa 2460

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct 2520

2461 caactacgcg accgtcacaa ggacgcggcc aacgtaagct aaggacaaac attaacagga 2520

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg 2580

2521 aaattgtcgc tagcgcataa agcagagcga gtccgcgtta gtgcttactt attgccaaac 2580

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa 2640

2581 caactacgct cactaaaact actgctcgca ttaccgaccg gacaacttgt tcagaccttt 2640

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca 2700

2641 ctttacgtat ttgaaaacgg taagagtggc ctaagtcagc agtgagtacc actaaagagt 2700

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc 2760

2701 gaactattgg aataaaaact gctccccttt aattatccaa cataactaca acctgctcag 2760

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 2820

2761 ccttagcgtc tggctatggt cctagaacgg taggatacct tgacggagcc actcaaaaga 2820

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 2880

2821 ggaagtaatg tctttgccga aaaagttttt ataccataac tattaggact atacttattt 2880

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa 2940

2881 aacgtcaaag taaactacga gctactcaaa aagattagtc ttaaccaatt aaccaacatt 2940

......... ORF 1 KanR2 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt 3000

2941 gtgaccgtct cgtaatgcga ctgaactgcc ctgccgcgtt cgagtactgg ttttagggaa 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 3060

3001 ttgcactcaa tgcgcagcaa ggtgactcgc agtctggggc atcttttcta gtttcctaga 3060

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 3120

3061 agaactctag gaaaaaaaga cgcgcattag acgacgaacg tttgtttttt tggtggcgat 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 3180

3121 ggtcgccacc aaacaaacgg cctagttctc gatggttgag aaaaaggctt ccattgaccg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 3240

3181 aagtcgtctc gcgtctatgg tttatgacaa gaagatcaca tcggcatcaa tccggtggtg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 3300

3241 aagttcttga gacatcgtgg cggatgtatg gagcgagacg attaggacaa tggtcaccga 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 3360

3301 cgacggtcac cgctattcag cacagaatgg cccaacctga gttctgctat caatggccta 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 3420

3361 ttccgcgtcg ccagcccgac ttgcccccca agcacgtgtg tcgggtcgaa cctcgcttgc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 3480

3421 tggatgtggc ttgactctat ggatgtcgca ctcgatactc tttcgcggtg cgaagggctt 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 3540

3481 ccctctttcc gcctgtccat aggccattcg ccgtcccagc cttgtcctct cgcgtgctcc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 3600

3541 ctcgaaggtc cccctttgcg gaccatagaa atatcaggac agcccaaagc ggtggagact 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 3660

3601 gaactcgcag ctaaaaacac tacgagcagt ccccccgcct cggatacctt tttgcggtcg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtt....... 3713

3661 ttgcgccgga aaaatgccaa ggaccggaaa acgaccggaa aacgagtgta caa....... 3713

.......... .......... .......... .......... .......... ..........