gj3gj3LOCUS pENTR/SD/D-TOPO 2601 bp DNA SYN 29-Aug-2007 DEFINITION pENTR/SD/D-TOPO ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..2601 /organism="pENTR/SD/D-TOPO" /mol_type="other DNA" terminator complement(268..295) /label="rrnB_T2_terminator" terminator complement(427..470) /label="rrnB_T1_terminator" misc_feature 522..544 /label="M13_pUC_fwd_primer" misc_feature 537..553 /label="M13_forward20_primer" misc_feature complement(569..668) /label="attL1" misc_feature 684..700 /label="T7_transl_en_RBS" misc_feature 726..825 /label="attL2" promoter complement(843..861) /label="T7_promoter" misc_feature complement(862..880) /label="M13_reverse_primer" CDS 995..1804 /label="KanR2" /gene="KanR2" /note="ORF frame 2" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" gene 995..1804 /label="KanR2" /gene="KanR2" rep_origin 1935..2554 /label="pBR322_origin" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCC GCGGCCGCCT TGTTTAACTT TAAGAAGGAG CCCTTCACCA AGGGTGGGCG 721 CGCCGACCCA GCTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG 781 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGATAT 841 CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC 901 TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAAATATA TCATCATGAA CAATAAAACT 961 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC 1021 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA 1081 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA 1141 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG 1201 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC 1261 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA 1321 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT 1381 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA 1441 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA 1501 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA 1561 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT 1621 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT 1681 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA 1741 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT 1801 CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA 1861 CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC 1921 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 1981 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 2041 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 2101 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 2161 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 2221 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 2281 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 2341 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 2401 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 2461 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 2521 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 2581 CTGGCCTTTT GCTCACATGT T //) pENTR/SD/D-TOPO "Times New Romangj3C) ' rrnB_T2_term"Times New Romangj3C)  rrnB_T1_term"Times New Romangj3CɆ)  M13_pUC_fwd_pri"Times New Romangj3) )M13_forward20_pri"Times New Romangj3C) 9attL1"Times New Romangj3C) T7_transl_en_RBS"Times New Romangj3CɆ) 9attL2"Times New Romangj3) K]T7_pro"Times New Romangj3C) ^pM13_reverse_pri"Times New Romangj3C)  ORF 2"Times New Romangj3CɆ)  KanR2"Times New Romangj3)   pBR322_origin"Times New Roman gj3HpaI(501),AflII(554),ApaI(567)"Times New Roman) Zm gj3SacII(672),NotI(672),EagI(672)"Times New Roman) iY}Z[Eq gj3EcoRV"Times New RomanF) Tfxx gj3NruI"Times New Roman5) #44LC gj3ApaLI"Times New Roman) |~ gj3test"Times New Roman%) bRBB7 gj3test"Times New Roman ) _Z>n>d