Sequence of pENTR_SD

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Sequence of pENTR_SD_D-TOPO

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Sequence of pENTR/SD/D-TOPO : 2601 BP; 654 A; 623 C; 640 G; 684 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 120

61 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 180

121 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 240

181 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattat gcgcatggcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 300

241 atcggtcctt ctcaaacatc tttgcgtttt tccggtaggc agtcctaccg gaagacgaat 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 360

301 caaactacgg accgtcaaat accgcccgca ggacgggcgg tgggaggccc ggcaacgaag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 420

361 tgttgcaagt ttaggcgagg gccgcctaaa caggatgagt cctctcgcaa gtggctgttt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 480

421 gttgtctatt ttgctttccg ggtcagaagg ctgactcgga aagcaaaata aactacggac 480

.......... ......... rrnB_T1_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 540

481 cgtcaaggga tgagagcgca attgcgatcg tacctacaaa agggtcagtg ctgcaacatt 540

.......... .......... .......... .......... ....... M13_pUC_fwd_pri

.......... AflII(554) ApaI(567) .......... .......... ..........

541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 600

541 ttgctgccgg tcagaattcg agcccggggt ttattactaa aataaaactg actatcactg 600

M13_forward20_pri .. .......... .......... attL1 ..........

.......... .......... .......... .......... .......... ..........

601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 660

601 gacaagcaac gttgtttaac tactcgttac gaaaaaatat tacggttgaa acatgttttt 660

.......... .......... attL1 .......... .......... ..........

SacII(672),NotI(672),EagI(672) .......... .......... ..........

661 agcaggctcc gcggccgcct tgtttaactt taagaaggag cccttcacca agggtgggcg 720

661 tcgtccgagg cgccggcgga acaaattgaa attcttcctc gggaagtggt tcccacccgc 720

.......... .......... ....... T7_transl_en_RBS ....... ..........

.......... .......... .......... .......... .......... ..........

721 cgccgaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg 780

721 gcggctgggt cgaaagaaca tgtttcaacc gtaatattct ttcgtaacga atagttaaac 780

.......... .......... .......... attL2 .......... ..........

.......... .......... .......... .......... .......... EcoRV(838)

781 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat 840

781 aacgttgctt gtccagtgat agtcagtttt attttagtaa taaacggtag gtcgactata 840

.......... .......... attL2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc 900

841 ggggatatca ctcagcataa tgtaccagta tcgacaaagg accgtcgaga ccgggcacag 900

.......... T7_pro ....... M13_reverse_pri ....... ..........

.......... .......... .......... .......... .......... ..........

901 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 960

901 agttttagag actacaatgt aacgtgttct atttttatat agtagtactt gttattttga 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 1020

961 cagacgaatg tatttgtcat tatgttcccc acaatactcg gtataagttg ccctttgcag 1020

.......... .......... .......... ......... ORF 2 KanR2 .........

.......... .......... .......... .......... .......... NruI(1077)

1021 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 1080

1021 ctccggcgct aatttaaggt tgtacctacg actaaatata cccatattta cccgagcgct 1080

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga 1140

1081 attacagccc gttagtccac gctgttagat agcgaacata cccttcgggc tacgcggtct 1140

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 1200

1141 caacaaagac tttgtaccgt ttccatcgca acggttacta caatgtctac tctaccagtc 1200

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 1260

1201 tgatttgacc gactgcctta aatacggaga aggctggtag ttcgtaaaat aggcatgagg 1260

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga 1320

1261 actactacgt accaatgagt ggtgacgcta ggggcctttt tgtcgtaagg tccataatct 1320

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 1380

1321 tcttatagga ctaagtccac ttttataaca actacgcgac cgtcacaagg acgcggccaa 1380

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 1440

1381 cgtaagctaa ggacaaacat taacaggaaa attgtcgcta gcgcataaag cagagcgagt 1440

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 1500

1441 ccgcgttagt gcttacttat tgccaaacca actacgctca ctaaaactac tgctcgcatt 1500

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 1560

1501 accgaccgga caacttgttc agacctttct ttacgtattt gaaaacggta agagtggcct 1560

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 1620

1561 aagtcagcag tgagtaccac taaagagtga actattggaa taaaaactgc tcccctttaa 1620

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 1680

1621 ttatccaaca taactacaac ctgctcagcc ttagcgtctg gctatggtcc tagaacggta 1680

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 1740

1681 ggataccttg acggagccac tcaaaagagg aagtaatgtc tttgccgaaa aagtttttat 1740

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 1800

1741 accataacta ttaggactat acttatttaa cgtcaaagta aactacgagc tactcaaaaa 1800

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga 1860

1801 gattagtctt aaccaattaa ccaacattgt gaccgtctcg taatgcgact gaactgccct 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc 1920

1861 gccgcgttcg agtactggtt ttagggaatt gcactcaatg cgcagcaagg tgactcgcag 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 1980

1921 tctggggcat cttttctagt ttcctagaag aactctagga aaaaaagacg cgcattagac 1980

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

1981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 2040

1981 gacgaacgtt tgtttttttg gtggcgatgg tcgccaccaa acaaacggcc tagttctcga 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 2100

2041 tggttgagaa aaaggcttcc attgaccgaa gtcgtctcgc gtctatggtt tatgacagga 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 2160

2101 agatcacatc ggcatcaatc cggtggtgaa gttcttgaga catcgtggcg gatgtatgga 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 2220

2161 gcgagacgat taggacaatg gtcaccgacg acggtcaccg ctattcagca cagaatggcc 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 2280

2221 caacctgagt tctgctatca atggcctatt ccgcgtcgcc agcccgactt gccccccaag 2280

.......... ........ pBR322_origin ........ .......... ..........

ApaLI(2281) ........ .......... .......... .......... ..........

2281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 2340

2281 cacgtgtgtc gggtcgaacc tcgcttgctg gatgtggctt gactctatgg atgtcgcact 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 2400

2341 cgtaactctt tcgcggtgcg aagggcttcc ctctttccgc ctgtccatag gccattcgcc 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2460

2401 gtcccagcct tgtcctctcg cgtgctccct cgaaggtccc cctttgcgga ccatagaaat 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2520

2461 atcaggacag cccaaagcgg tggagactga actcgcagct aaaaacacta cgagcagtcc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2580

2521 ccccgcctcg gatacctttt tgcggtcgtt gcgccggaaa aatgccaagg accggaaaac 2580

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctggcctttt gctcacatgt t......... .......... .......... .......... 2601

2581 gaccggaaaa cgagtgtaca a......... .......... .......... .......... 2601

.......... .......... .......... .......... .......... ..........