Sequence of pENTR_TEV

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Sequence of pENTR_TEV_D-TOPO

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Sequence of pENTR/TEV/D-TOPO : 2610 BP; 655 A; 627 C; 643 G; 685 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 120

61 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 180

121 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 240

181 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattat gcgcatggcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 300

241 atcggtcctt ctcaaacatc tttgcgtttt tccggtaggc agtcctaccg gaagacgaat 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 360

301 caaactacgg accgtcaaat accgcccgca ggacgggcgg tgggaggccc ggcaacgaag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 420

361 tgttgcaagt ttaggcgagg gccgcctaaa caggatgagt cctctcgcaa gtggctgttt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 480

421 gttgtctatt ttgctttccg ggtcagaagg ctgactcgga aagcaaaata aactacggac 480

.......... ......... rrnB_T1_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 540

481 cgtcaaggga tgagagcgca attgcgatcg tacctacaaa agggtcagtg ctgcaacatt 540

.......... .......... .......... .......... ....... M13_pUC_fwd_pri

.......... AflII(554) ApaI(567) .......... .......... ..........

541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 600

541 ttgctgccgg tcagaattcg agcccggggt ttattactaa aataaaactg actatcactg 600

M13_forward20_pri .. .......... .......... attL1 ..........

.......... .......... .......... .......... .......... ..........

601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 660

601 gacaagcaac gttgtttaac tactcgttac gaaaaaatat tacggttgaa acatgttttt 660

.......... .......... attL1 .......... .......... ..........

SacII(672),NotI(672),EagI(672) XhoI(683) .......... ..........

661 agcaggctcc gcggccgccg tactcgagga aaacctgtat tttcagggct ccttcaccaa 720

661 tcgtccgagg cgccggcggc atgagctcct tttggacata aaagtcccga ggaagtggtt 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gggtgggcgc gccgacccag ctttcttgta caaagttggc attataagaa agcattgctt 780

721 cccacccgcg cggctgggtc gaaagaacat gtttcaaccg taatattctt tcgtaacgaa 780

.......... .......... .......... attL2 .......... ..........

.......... .......... .......... .......... .......... ..........

781 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc 840

781 tagttaaaca acgttgcttg tccagtgata gtcagtttta ttttagtaat aaacggtagg 840

.......... .......... attL2 .......... .......... ..........

EcoRV(847) .......... .......... .......... .......... ..........

841 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg 900

841 tcgactatag gggatatcac tcagcataat gtaccagtat cgacaaagga ccgtcgagac 900

.......... ....... T7_pro M13_reverse_pri ....... ..........

.......... .......... .......... .......... .......... ..........

901 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac 960

901 cgggcacaga gttttagaga ctacaatgta acgtgttcta tttttatata gtagtacttg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg 1020

961 ttattttgac agacgaatgt atttgtcatt atgttcccca caatactcgg tataagttgc 1020

.......... .......... .......... .......... ......... ORF 2 KanR2

.......... .......... .......... .......... .......... ..........

1021 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg 1080

1021 cctttgcagc tccggcgcta atttaaggtt gtacctacga ctaaatatac ccatatttac 1080

.......... ......... ORF 2 KanR2 ......... .......... ..........

NruI(1086) .......... .......... .......... .......... ..........

1081 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga 1140

1081 ccgagcgcta ttacagcccg ttagtccacg ctgttagata gcgaacatac ccttcgggct 1140

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga 1200

1141 acgcggtctc aacaaagact ttgtaccgtt tccatcgcaa cggttactac aatgtctact 1200

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat 1260

1201 ctaccagtct gatttgaccg actgccttaa atacggagaa ggctggtagt tcgtaaaata 1260

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca 1320

1261 ggcatgagga ctactacgta ccaatgagtg gtgacgctag gggccttttt gtcgtaaggt 1320

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct 1380

1321 ccataatctt cttataggac taagtccact tttataacaa ctacgcgacc gtcacaagga 1380

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg 1440

1381 cgcggccaac gtaagctaag gacaaacatt aacaggaaaa ttgtcgctag cgcataaagc 1440

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga 1500

1441 agagcgagtc cgcgttagtg cttacttatt gccaaaccaa ctacgctcac taaaactact 1500

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt 1560

1501 gctcgcatta ccgaccggac aacttgttca gacctttctt tacgtatttg aaaacggtaa 1560

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga 1620

1561 gagtggccta agtcagcagt gagtaccact aaagagtgaa ctattggaat aaaaactgct 1620

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga 1680

1621 cccctttaat tatccaacat aactacaacc tgctcagcct tagcgtctgg ctatggtcct 1680

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt 1740

1681 agaacggtag gataccttga cggagccact caaaagagga agtaatgtct ttgccgaaaa 1740

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga 1800

1741 agtttttata ccataactat taggactata cttatttaac gtcaaagtaa actacgagct 1800

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac 1860

1801 actcaaaaag attagtctta accaattaac caacattgtg accgtctcgt aatgcgactg 1860

ORF 2 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca 1920

1861 aactgccctg ccgcgttcga gtactggttt tagggaattg cactcaatgc gcagcaaggt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1980

1921 gactcgcagt ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc 1980

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1981 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 2040

1981 gcattagacg acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 2100

2041 agttctcgat ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 2160

2101 atgacaggaa gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 2220

2161 atgtatggag cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 2280

2221 agaatggccc aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg 2280

.......... ........ pBR322_origin ........ .......... ..........

ApaLI(2290) ........ .......... .......... .......... ..........

2281 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 2340

2281 ccccccaagc acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 2400

2341 tgtcgcactc gtaactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2460

2401 ccattcgccg tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2520

2461 catagaaata tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2580

2521 gagcagtccc cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggccttttgc tggccttttg ctcacatgtt .......... .......... .......... 2610

2581 ccggaaaacg accggaaaac gagtgtacaa .......... .......... .......... 2610

.......... .......... .......... .......... .......... ..........