Sequence of pENTR

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Sequence of pENTR_U6

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Sequence of pENTR/U6 : 2854 BP; 745 A; 651 C; 683 G; 775 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 60

1 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 120

61 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 180

121 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 240

181 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattat gcgcatggcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 300

241 atcggtcctt ctcaaacatc tttgcgtttt tccggtaggc agtcctaccg gaagacgaat 300

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 360

301 caaactacgg accgtcaaat accgcccgca ggacgggcgg tgggaggccc ggcaacgaag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 420

361 tgttgcaagt ttaggcgagg gccgcctaaa caggatgagt cctctcgcaa gtggctgttt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 480

421 gttgtctatt ttgctttccg ggtcagaagg ctgactcgga aagcaaaata aactacggac 480

.......... ......... rrnB_T1_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 540

481 cgtcaaggga tgagagcgca attgcgatcg tacctacaaa agggtcagtg ctgcaacatt 540

.......... .......... .......... .......... ....... M13_pUC_fwd_pri

.......... AflII(554) ApaI(567) .......... .......... ..........

541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 600

541 ttgctgccgg tcagaattcg agcccggggt ttattactaa aataaaactg actatcactg 600

M13_forward20_pri .. .......... .......... attL1 ..........

.......... .......... .......... .......... .......... ..........

601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 660

601 gacaagcaac gttgtttaac tactcgttac gaaaaaatat tacggttgaa acatgttttt 660

.......... .......... attL1 .......... .......... ..........

.......... .......... SalI(686) BamHI(693) KpnI(703) ..........

661 agcaggcttt aaaggaacca attcagtcga ctggatccgg taccaaggtc gggcaggaag 720

661 tcgtccgaaa tttccttggt taagtcagct gacctaggcc atggttccag cccgtccttc 720

.......... .......... .......... .......... .......... hU6_pro

.......... .......... .......... .......... .......... ..........

721 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga 780

721 tcccggataa agggtactaa ggaagtataa acgtatatgc tatgttccga caatctctct 780

.......... .......... hU6_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa 840

781 attaatctta attaaactga catttgtgtt tctataatca tgttttatgc actgcatctt 840

.......... .......... hU6_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata 900

841 tcattattaa agaacccatc aaacgtcaaa attttaatac aaaattttac ctgatagtat 900

.......... .......... hU6_pro .......... .......... LKO1_5_pri

.......... .......... .......... .......... .......... ..........

901 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac 960

901 acgaatggca ttgaactttc ataaagctaa agaaccgaaa tatatagaac acctttcctg 960

LKO1_5_pri .......... hU6_pro .......... .......... ..........

.......... XbaI(974) .......... .......... .......... ..........

961 gaaacacctt ttttctagac ccagctttct tgtacaaagt tggcattata agaaagcatt 1020

961 ctttgtggaa aaaagatctg ggtcgaaaga acatgtttca accgtaatat tctttcgtaa 1020

.......... .......... .......... attL2 .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc 1080

1021 cgaatagtta aacaacgttg cttgtccagt gatagtcagt tttattttag taataaacgg 1080

.......... .......... attL2 .......... .......... ..........

......... EcoRV(1091) ......... .......... .......... ..........

1081 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc 1140

1081 taggtcgact ataggggata tcactcagca taatgtacca gtatcgacaa aggaccgtcg 1140

.......... .......... T7_pro ....... M13_reverse_pri .......

.......... .......... .......... .......... .......... ..........

1141 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat 1200

1141 agaccgggca cagagtttta gagactacaa tgtaacgtgt tctattttta tatagtagta 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc 1260

1201 cttgttattt tgacagacga atgtatttgt cattatgttc cccacaatac tcggtataag 1260

.......... .......... .......... .......... ......... ORF 3 KanR2

.......... .......... .......... .......... .......... ..........

1261 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata 1320

1261 ttgccctttg cagctccggc gctaatttaa ggttgtacct acgactaaat atacccatat 1320

.......... ......... ORF 3 KanR2 ......... .......... ..........

NruI(1330) .......... .......... .......... .......... ..........

1321 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc 1380

1321 ttacccgagc gctattacag cccgttagtc cacgctgtta gatagcgaac atacccttcg 1380

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag 1440

1381 ggctacgcgg tctcaacaaa gactttgtac cgtttccatc gcaacggtta ctacaatgtc 1440

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt 1500

1441 tactctacca gtctgatttg accgactgcc ttaaatacgg agaaggctgg tagttcgtaa 1500

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat 1560

1501 aataggcatg aggactacta cgtaccaatg agtggtgacg ctaggggcct ttttgtcgta 1560

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt 1620

1561 aggtccataa tcttcttata ggactaagtc cacttttata acaactacgc gaccgtcaca 1620

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat 1680

1621 aggacgcggc caacgtaagc taaggacaaa cattaacagg aaaattgtcg ctagcgcata 1680

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg 1740

1681 aagcagagcg agtccgcgtt agtgcttact tattgccaaa ccaactacgc tcactaaaac 1740

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc 1800

1741 tactgctcgc attaccgacc ggacaacttg ttcagacctt tctttacgta tttgaaaacg 1800

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg 1860

1801 gtaagagtgg cctaagtcag cagtgagtac cactaaagag tgaactattg gaataaaaac 1860

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc 1920

1861 tgctcccctt taattatcca acataactac aacctgctca gccttagcgt ctggctatgg 1920

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc 1980

1921 tcctagaacg gtaggatacc ttgacggagc cactcaaaag aggaagtaat gtctttgccg 1980

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc 2040

1981 aaaaagtttt tataccataa ctattaggac tatacttatt taacgtcaaa gtaaactacg 2040

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc 2100

2041 agctactcaa aaagattagt cttaaccaat taaccaacat tgtgaccgtc tcgtaatgcg 2100

ORF 3 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt 2160

2101 actgaactgc cctgccgcgt tcgagtactg gttttaggga attgcactca atgcgcagca 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 2220

2161 aggtgactcg cagtctgggg catcttttct agtttcctag aagaactcta ggaaaaaaag 2220

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2221 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 2280

2221 acgcgcatta gacgacgaac gtttgttttt ttggtggcga tggtcgccac caaacaaacg 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 2340

2281 gcctagttct cgatggttga gaaaaaggct tccattgacc gaagtcgtct cgcgtctatg 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 2400

2341 gtttatgaca ggaagatcac atcggcatca atccggtggt gaagttcttg agacatcgtg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 2460

2401 gcggatgtat ggagcgagac gattaggaca atggtcaccg acgacggtca ccgctattca 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 2520

2461 gcacagaatg gcccaacctg agttctgcta tcaatggcct attccgcgtc gccagcccga 2520

.......... ........ pBR322_origin ........ .......... ..........

......... ApaLI(2534) ......... .......... .......... ..........

2521 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 2580

2521 cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg ctggatgtgg cttgactcta 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 2640

2581 tggatgtcgc actcgtaact ctttcgcggt gcgaagggct tccctctttc cgcctgtcca 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 2700

2641 taggccattc gccgtcccag ccttgtcctc tcgcgtgctc cctcgaaggt ccccctttgc 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 2760

2701 ggaccataga aatatcagga cagcccaaag cggtggagac tgaactcgca gctaaaaaca 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 2820

2761 ctacgagcag tccccccgcc tcggatacct ttttgcggtc gttgcgccgg aaaaatgcca 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcctggcctt ttgctggcct tttgctcaca tgtt...... .......... .......... 2854

2821 aggaccggaa aacgaccgga aaacgagtgt acaa...... .......... .......... 2854

.......... .......... .......... .......... .......... ..........