Sequence of pET-11

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Sequence of pET-11_a

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Sequence of pET-11 a : 11371 BP; 2631 A; 3047 C; 3046 G; 2632 T; 15 other;

.......... .......... .......... .......... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

T7_pro .......... .......... lacO .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctca tcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.... T7_transl_en_RBS Xpress_fwd_pri T7_gene10_leader T7_leader

BamHI(125) .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240

181 cgccctatag gcctatatca aggaggaaag tcgttttttg gggagttctg ggcaaatctc 240

T7_Terminal_pri .... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300

241 cggggttccc caatacgatc aataacgagt cgccaccgtc gtcggttgag tcgaaggaaa 300

.......... T7_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cgggctttgt tagcagccgg atccgcgacc catttgctgt ccaccagtca tgctagccat 360

301 gcccgaaaca atcgtcggcc taggcgctgg gtaaacgaca ggtggtcagt acgatcggta 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 atgtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct 420

361 tacatataga ggaagaattt caatttgttt taataaagat ctccccttaa caataggcga 420

.......... .......... .......... .......... .......... ..........

ClaI(422) .......... .......... .......... .......... ..........

421 cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg 480

421 gtgttaaggg gatatcactc agcataatta aagcgcccta gctctagagc taggagatgc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AatII(525) ..........

481 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg 540

481 ggcctgcgta gcaccggccg tagtggccgc ggtgtccacg ccaacgaccg cggatatagc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg 600

541 ggctgtagtg gctacccctt ctagcccgag cggtgaagcc cgagtactcg cgaacaaagc 600

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

601 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg 660

601 cgcacccata ccaccgtccg gggcaccggc cccctgacaa cccgcggtag aggaacgtac 660

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa 720

661 gtggtaagga acgccgccgc cacgagttgc cggagttgga tgatgacccg acgaaggatt 720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc 780

721 acgtcctcag cgtattccct ctcgcagctc tagggcctgt ggtagcttac cgcgttttgg 780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa 840

781 aaagcgccat accgtactat cgcgggcctt ctctcagtta agtcccacca cttacacttt 840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 900

841 ggtcattgca atatgctaca gcgtctcata cggccacaga gaatagtctg gcaaagggcg 900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 960

901 caccacttgg tccggtcggt gcaaagacgc ttttgcgccc tttttcacct tcgccgctac 960

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg 1020

961 cgcctcgact taatgtaagg gttggcgcac cgtgttgttg accgcccgtt tgtcagcaac 1020

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg 1080

1021 gactaaccgc aacggtggag gtcagaccgg gacgtgcgcg gcagcgttta acagcgccgc 1080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 1140

1081 taatttagag cgcggctagt tgacccacgg tcgcaccacc acagctacca tcttgcttcg 1140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 1200

1141 ccgcagcttc ggacatttcg ccgccacgtg ttagaagagc gcgttgcgca gtcacccgac 1200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

PstI(1202) .......... .......... .......... .......... ..........

1201 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 1260

1201 tagtaattga taggcgacct actggtccta cggtaacgac accttcgacg gacgtgatta 1260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 1320

1261 caaggccgca ataaagaact acagagactg gtctgtgggt agttgtcata ataaaagagg 1320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 1380

1321 gtacttctgc catgcgctga cccgcacctc gtagaccagc gtaacccagt ggtcgtttag 1380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 1440

1381 cgcgacaatc gcccgggtaa ttcaagacag agccgcgcag acgcagaccg accgaccgta 1440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 1500

1441 tttatagagt gagcgttagt ttaagtcggc tatcgccttg cccttccgct gacctcacgg 1500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 1560

1501 tacaggccaa aagttgtttg gtacgtttac gacttactcc cgtagcaagg gtgacgctac 1560

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 1620

1561 gaccaacggt tgctagtcta ccgcgacccg cgttacgcgc ggtaatggct caggcccgac 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 1680

1621 gcgcaaccac gcctatagag ccatcaccct atgctgctat ggcttctgtc gagtacaata 1680

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1681 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 1740

1681 tagggcggca attggtggta gtttgtccta aaagcggacg accccgtttg gtcgcacctg 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 1800

1741 gcgaacgacg ttgagagagt cccggtccgc cacttcccgt tagtcgacaa cgggcagagt 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 1860

1801 gaccactttt ctttttggtg ggaccgcggg ttatgcgttt ggcggagagg ggcgcgcaac 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 1920

1861 cggctaagta attacgtcga ccgtgctgtc caaagggctg acctttcgcc cgtcactcgc 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt 1980

1921 gttgcgttaa ttacattcaa tcgagtgagt aatccgtggc cctagagctg gctacgggaa 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 2040

1981 ctctcggaag ttgggtcagt cgaggaaggc cacccgcgcc ccgtactgat agcagcggcg 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt 2100

2041 tgaatactga cagaagaaat agtacgttga gcatcctgtc cacggccgtc gcgagaccca 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt 2160

2101 gtaaaagccg ctcctggcga aagcgacctc gcgctgctac tagccggaca gcgaacgcca 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt 2220

2161 taagccttag aacgtgcggg agcgagttcg gaagcagtga ccagggcggt ggtttgcaaa 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct 2280

2221 gccgctcttc gtccggtaat agcggccgta ccgccggctg cgcgacccga tgcagaacga 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg 2340

2281 ccgcaagcgc tgcgctccga cctaccggaa ggggtaatac taagaagagc gaaggccgcc 2340

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg 2400

2341 gtagccctac gggcgcaacg tccggtacga caggtccgtc catctactgc tggtagtccc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat 2460

2401 tgtcgaagtt cctagcgagc gccgagaatg gtcggattga agctagtgac ctggcgacta 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg 2520

2461 gcagtgccgc taaatacggc ggagccgctc gtgtaccttg cccaaccgta cctaacatcc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac 2580

2521 gcggcgggat atggaacaga cggaggggcg caacgcagcg ccacgtacct cggcccggtg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga 2640

2581 gagctggact taccttcggc cgccgtggag cgattgccta agtggtgagg ttcttaacct 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc 2700

2641 cggttagtta agaacgcctc ttgacactta cgcgtttggt tgggaaccgt cttgtatagg 2700

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

2701 atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg 2760

2701 tagcgcaggc ggtagaggtc gtcggcgtgc gccgcgtaga gcccgtcgca acccaggacc 2760

pGEX_3_pri .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc 2820

2761 ggtgcccacg cgtactagca cgaggacagc aactcctggg ccgatccgac cgccccaacg 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc 2880

2821 gaatgaccaa tcgtcttact tagtggctat gcgctcgctt gcacttcgct gacgacgacg 2880

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt 2940

2881 ttttgcagac gctggactcg ttgttgtact taccagaagc caaaggcaca aagcatttca 2940

ROP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc 3000

2941 gacctttgcg ccttcagtcg cgggacgtgg taatacaagg cctagacgta gcgtcctacg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga 3060

3001 acgaccgatg ggacaccttg tggatgtaga cataattgct tcgcgaccgt aactgggact 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc 3120

3061 cactaaaaag agaccagggc ggcgtaggta tggcggtcaa caaatgggag tgttgcaagg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc 3180

3121 tcattggccc gtacaagtag tagtcattgg gcatagcact cgtaggagag agcaaagtag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag 3240

3181 ccatagtaat gggggtactt gtctttaggg ggaatgtgcc tccgtagtca ctggtttgtc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag 3300

3241 cttttttggc gggaattgta ccgggcgaaa tagtcttcgg tctgtaattg cgaagacctc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac 3360

3301 tttgagttgc tcgacctgcg cctacttgtc cgtctgtaga cacttagcga agtgctggtg 3360

.......... .......... .......... .......... .......... ..........

MscI(3365) .......... .......... .......... .......... ..........

3361 gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 3420

3361 cgactactcg aaatggcgtc gacggagcgc gcaaagccac tactgccact tttggagact 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 3480

3421 gtgtacgtcg agggcctctg ccagtgtcga acagacattc gcctacggcc ctcgtctgtt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 3540

3481 cgggcagtcc cgcgcagtcg cccacaaccg cccacagccc cgcgtcggta ctgggtcagt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga 3600

3541 gcatcgctat cgcctcacat atgaccgaat tgatacgccg tagtctcgtc taacatgact 3600

.......... ....... tet(0-612) ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gagtgcacca tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 3660

3601 ctcacgtggt atatacgcca cactttatgg cgtgtctacg cattcctctt ttatggcgta 3660

.......... ....... tet(0-612) ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 3720

3661 gtccgcgaga aggcgaagga gcgagtgact gagcgacgcg agccagcaag ccgacgccgc 3720

.......... ....... tet(0-612) ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 3780

3721 tcgccatagt cgagtgagtt tccgccatta tgccaatagg tgtcttagtc ccctattgcg 3780

.......... ....... tet(0-612) ORF 2 ORF 2 ....... ..........

.......... .......... .......... .......... .......... ..........

3781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 3840

3781 tcctttcttg tacactcgtt ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa 3840

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... EagI(3868) .......... .......... ..........

3841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 3900

3841 cgaccgcaaa aaggtatccg aggcgggggg actgctcgta gtgtttttag ctgcgagttc 3900

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 3960

3901 agtctccacc gctttgggct gtcctgatat ttctatggtc cgcaaagggg gaccttcgag 3960

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4020

3961 ggagcacgcg agaggacaag gctgggacgg cgaatggcct atggacaggc ggaaagaggg 4020

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 4080

4021 aagcccttcg caccgcgaaa gagtatcgag tgcgacatcc atagagtcaa gccacatcca 4080

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4140

4081 gcaagcgagg ttcgacccga cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa 4140

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4200

4141 taggccattg atagcagaac tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg 4200

ORF 2 .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4260

4201 tcggtgacca ttgtcctaat cgtctcgctc catacatccg ccacgatgtc tcaagaactt 4260

.......... .......... ORF 2 ORF 3 lacI .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 4320

4261 caccaccgga ttgatgccga tgtgatcttc ctgtcataaa ccatagacgc gagacgactt 4320

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4380

4321 cggtcaatgg aagccttttt ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc 4380

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... HpaI(4434)

4381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4440

4381 atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct 4440

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4500

4441 tctaggaaac tagaaaagat gccccagact gcgagtcacc ttgcttttga gtgcaattcc 4500

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4560

4501 ctaaaaccag tactctaata gtttttccta gaagtggatc taggaaaatt taatttttac 4560

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 4620

4561 ttcaaaattt agttagattt catatatact catttgaacc agactgtcaa tggttacgaa 4620

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 4680

4621 ttagtcactc cgtggataga gtcgctagac agataaagca agtaggtatc aacggactga 4680

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ApaI(4733)

4681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 4740

4681 ggggcagcac atctattgat gctatgccct cccgaatggt agaccggggt cacgacgtta 4740

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 4800

4741 ctatggcgct ctgggtgcga gtggccgagg tctaaatagt cgttatttgg tcggtcggcc 4800

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 4860

4801 ttcccggctc gcgtcttcac caggacgttg aaataggcgg aggtaggtca gataattaac 4860

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 4920

4861 aacggccctt cgatctcatt catcaagcgg tcaattatca aacgcgttgc aacaacggta 4920

.......... .......... ORF 3 lacI .......... .......... ..........

BclI(4922) .......... .......... .......... .......... ..........

4921 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4980

4921 acgacgtccg tagcaccaca gtgcgagcag caaaccatac cgaagtaagt cgaggccaag 4980

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5040

4981 ggttgctagt tccgctcaat gtactagggg gtacaacacg ttttttcgcc aatcgaggaa 5040

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5100

5041 gccaggaggc tagcaacagt cttcattcaa ccggcgtcac aatagtgagt accaataccg 5100

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5160

5101 tcgtgacgta ttaagagaat gacagtacgg taggcattct acgaaaagac actgaccact 5160

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5220

5161 catgagttgg ttcagtaaga ctcttatcac atacgccgct ggctcaacga gaacgggccg 5220

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5280

5221 cagttgtgcc ctattatggc gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt 5280

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5340

5281 tgcaagaagc cccgcttttg agagttccta gaatggcgac aactctaggt caagctacat 5340

lacI .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5400

5341 tgggtgagca cgtgggttga ctagaagtcg tagaaaatga aagtggtcgc aaagacccac 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5460

5401 tcgtttttgt ccttccgttt tacggcgttt tttcccttat tcccgctgtg cctttacaac 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5520

5461 ttatgagtat gagaaggaaa aagttataat aacttcgtaa atagtcccaa taacagagta 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 5580

5521 ctcgcctatg tataaactta cataaatctt tttatttgtt tatccccaag gcgcgtgtaa 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 5640

5581 aggggctttt cacggtggac tgcagattct ttggtaataa tagtactgta attggatatt 5640

pBRrevBam_pri ...... .......... .......... .......... ..........

......... BglII(5658) ......... .......... .......... ..........

5641 aaataggcgt atcacgaggc cctttcgtct tcaagaarev ersecomple mentttcttg 5700

5641 tttatccgca tagtgctccg ggaaagcaga agttcttyeb eysegokple kenaaagaac 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 5760

5701 ttctgctttc ccggagcact atgcggataa aaatatccaa ttacagtact attattacca 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 5820

5761 aagaatctgc agtccaccgt gaaaagcccc tttacacgcg ccttggggat aaacaaataa 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 5880

5821 aaagatttat gtaagtttat acataggcga gtactctgtt attgggacta tttacgaagt 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 5940

5881 tattataact ttttccttct catactcata agttgtaaag gcacagcggg aataagggaa 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 6000

5941 aaaacgccgt aaaacggaag gacaaaaacg agtgggtctt tgcgaccact ttcattttct 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 6060

6001 acgacttcta gtcaacccac gtgctcaccc aatgtagctt gacctagagt tgtcgccatt 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 6120

6061 ctaggaactc tcaaaagcgg ggcttcttgc aaaaggttac tactcgtgaa aatttcaaga 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 6180

6121 cgatacaccg cgccataata gggcacaact gcggcccgtt ctcgttgagc cagcggcgta 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 6240

6181 tgtgataaga gtcttactga accaactcat gagtggtcag tgtcttttcg tagaatgcct 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 6300

6241 accgtactgt cattctctta atacgtcacg acggtattgg tactcactat tgtgacgccg 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 6360

6301 gttgaatgaa gactgttgct agcctcctgg cttcctcgat tggcgaaaaa acgtgttgta 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 6420

6361 ccccctagta cattgagcgg aactagcaac ccttggcctc gacttacttc ggtatggttt 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac 6480

6421 gctgctcgca ctgtggtgct acggacgtcg ttaccgttgt tgcaacgcgt ttgataattg 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 6540

6481 accgcttgat gaatgagatc gaagggccgt tgttaattat ctgacctacc tccgcctatt 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 6600

6541 tcaacgtcct ggtgaagacg cgagccggga aggccgaccg accaaataac gactatttag 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 6660

6601 acctcggcca ctcgcaccca gagcgccata gtaacgtcgt gaccccggtc taccattcgg 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 6720

6661 gagggcatag catcaataga tgtgctgccc ctcagtccgt tgatacctac ttgctttatc 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 6780

6721 tgtctagcga ctctatccac ggagtgacta attcgtaacc attgacagtc tggttcaaat 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 6840

6781 gagtatatat gaaatctaac taaattttga agtaaaaatt aaattttcct agatccactt 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 6900

6841 ctaggaaaaa ctattagagt actggtttta gggaattgca ctcaaaagca aggtgactcg 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 6960

6901 cagtctgggg catcttttct agtttcctag aagaactcta ggaaaaaaag acgcgcatta 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 7020

6961 gacgacgaac gtttgttttt ttggtggcga tggtcgccac caaacaaacg gcctagttct 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 7080

7021 cgatggttga gaaaaaggct tccattgacc gaagtcgtct cgcgtctatg gtttatgaca 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 7140

7081 ggaagatcac atcggcatca atccggtggt gaagttcttg agacatcgtg gcggatgtat 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 7200

7141 ggagcgagac gattaggaca atggtcaccg acgacggtca ccgctattca gcacagaatg 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 7260

7201 gcccaacctg agttctgcta tcaatggcct attccgcgtc gccagcccga cttgcccccc 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 7320

7261 aagcacgtgt gtcgggtcga acctcgcttg ctggatgtgg cttgactcta tggatgtcgc 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 7380

7321 actcgatact ctttcgcggt gcgaagggct tccctctttc cgcctgtcca taggccattc 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 7440

7381 gccgtcccag ccttgtcctc tcgcgtgctc cctcgaaggt ccccctttgc ggaccataga 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 7500

7441 aatatcagga cagcccaaag cggtggagac tgaactcgca gctaaaaaca ctacgagcag 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 7560

7501 tccccccgcc tcggatacct ttttgcggtc gttgcgccgg aaaaatgcca aggaccggaa 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 7620

7561 aacgaccgga aaacgagtgt acaagaaagg acgcaatagg ggactaagac acctattggc 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 7680

7621 ataatggcgg aaactcactc gactatggcg agcggcgtcg gcttgctggc tcgcgtcgct 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg 7740

7681 cagtcactcg ctccttcgcc ttctcgcgga ctacgccata aaagaggaat gcgtagacac 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag 7800

7741 gccataaagt gtggcgtata taccacgtga gagtcatgtt agacgagact acggcgtatc 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc 7860

7801 aattcggtca tatgtgaggc gatagcgatg cactgaccca gtaccgacgc ggggctgtgg 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 7920

7861 gcggttgtgg gcgactgcgc gggactgccc gaacagacga gggccgtagg cgaatgtctg 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 7980

7921 ttcgacactg gcagaggccc tcgacgtaca cagtctccaa aagtggcagt agtggctttg 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg 8040

7981 cgcgctccgt cgacgccatt tcgagtagtc gcaccagcac ttcgctaagt gtctacagac 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga 8100

8041 ggacaagtag gcgcaggtcg agcaactcaa agaggtcttc gcaattacag accgaagact 8100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag 8160

8101 atttcgcccg gtacaattcc cgccaaaaaa ggacaaacca gtgactacgg aggcacattc 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac 8220

8161 cccctaaaga caagtacccc cattactatg gctactttgc tctctcctac gagtgctatg 8220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg 8280

8221 cccaatgact actacttgta cgggccaatg accttgcaac actcccattt gttgaccgcc 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata 8340

8281 atacctacgc cgccctggtc tctttttagt gagtcccagt tacggtcgcg aagcaattat 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa 8400

8341 gtctacatcc acaaggtgtc ccatcggtcg tcgtaggacg ctacgtctag gccttgtatt 8400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca 8460

8401 accacgtccc gcgactgaag gcgcaaaggt ctgaaatgct ttgtgccttt ggcttctggt 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc 8520

8461 aagtacaaca acgagtccag cgtctgcaaa acgtcgtcgt cagcgaagtg caagcgagcg 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca 8580

8521 catagccact aagtaagacg attggtcatt ccgttggggc ggtcggatcg gcccaggagt 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc 8640

8581 tgctgtcctc gtgctagtac gcgtgggcac cggtcctggg ttgcgacggg ctctacgcgg 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg 8700

8641 cgcacgccga cgacctctac cgcctgcgct acctatacaa gacggttccc aaccaaacgc 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag 8760

8701 gtaagtgtca agaggcgttc ttaactaacc gaggttaaga acctcaccac ttaggcaatc 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa 8820

8761 gctccacggc ggccgaaggt aagtccagct ccaccgggcc gaggtacgtg gcgctgcgtt 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt 8880

8821 gcgcccctcc gtctgttcca tatcccgccg cggatgttag gtacggttgg gcaaggtaca 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct 8940

8881 cgagcggctc cgccgtattt agcggcactg ctagtcgcca ggtcactagc ttcaatccga 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga 9000

8941 ccattctcgg cgctcgctag gaacttcgac agggactacc agcagtagat ggacggacct 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg 9060

9001 gtcgtaccgg acgttgcgcc cgtagggcta cggcggcctt cgctcttctt agtattaccc 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc 9120

9061 cttccggtag gtcggagcgc agcgcttgcg gtcgttctgc atcgggtcgc gcagccggcg 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 9180

9121 gtacggccgc tattaccgga cgaagagcgg ctttgcaaac caccgccctg gtcactgctt 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 9240

9181 ccgaactcgc tcccgcacgt tctaaggctt atggcgttcg ctgtccggct agtagcagcg 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 9300

9241 cgaggtcgct ttcgccagga gcggctttta ctgggtctcg cgacggccgt ggacaggatg 9300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 9360

9301 ctcaacgtac tatttcttct gtcagtattc acgccgctgc tatcagtacg gggcgcgggt 9360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 9420

9361 ggccttcctc gactgaccca acttccgaga gttcccgtag ccagctctag ggccacggat 9420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 9480

9421 tactcactcg attgaatgta attaacgcaa cgcgagtgac gggcgaaagg tcagcccttt 9480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 9540

9481 ggacagcacg gtcgacgtaa ttacttagcc ggttgcgcgc ccctctccgc caaacgcata 9540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 9600

9541 acccgcggtc ccaccaaaaa gaaaagtggt cactctgccc gttgtcgact aacgggaagt 9600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 9660

9601 ggcggaccgg gactctctca acgtcgttcg ccaggtgcga ccaaacgggg tcgtccgctt 9660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 9720

9661 ttaggacaaa ctaccaccaa ttgccgccct atattgtact cgacagaagc catagcagca 9720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 9780

9721 tagggtgatg gctctatagg cgtggttgcg cgtcgggcct gagccattac cgcgcgtaac 9780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 9840

9781 gcgggtcgcg gtagactagc aaccgttggt cgtagcgtca cccttgctac gggagtaagt 9840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 9900

9841 cgtaaacgta ccaaacaact tttggcctgt accgtgaggt cagcggaagg gcaaggcgat 9900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 9960

9901 agccgactta aactaacgct cactctataa atacggtcgg tcggtctgcg tctgcgcggc 9960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 10020

9961 tctgtcttga attacccggg cgattgtcgc gctaaacgac cactgggtta cgctggtcta 10020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 10080

10021 cgaggtgcgg gtcagcgcat ggcagaagta ccctctttta ttatgacaac tacccacaga 10080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 10140

10081 ccagtctctg tagttcttta ttgcggcctt gtaatcacgt ccgtcgaagg tgtcgttacc 10140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 10200

10141 gtaggaccag taggtcgcct atcaattact agtcgggtga ctgcgcaacg cgctcttcta 10200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 10260

10201 acacgtggcg gcgaaatgtc cgaagctgcg gcgaagcaag atggtagctg tggtggtgcg 10260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 10320

10261 accgtgggtc aactagccgc gctctaaatt agcggcgctg ttaaacgctg ccgcgcacgt 10320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 10380

10321 cccggtctga cctccaccgt tgcggttagt cgttgctgac aaacgggcgg tcaacaacac 10380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 10440

10381 ggtgcgccaa cccttacatt aagtcgaggc ggtagcggcg aaggtgaaaa agggcgcaaa 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 10500

10441 agcgtctttg caccgaccgg accaagtggt gcgccctttg ccagactatt ctctgtggcc 10500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 10560

10501 gtatgagacg ctgtagcata ttgcaatgac caaagtgtaa gtggtgggac ttaactgaga 10560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 10620

10561 gaaggcccgc gatagtacgg tatggcgctt tccaaaacgc ggtaagctac cacaggccct 10620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 10680

10621 agagctgcga gagggaatac gctgaggacg taatccttcg tcgggtcatc atccaactcc 10680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 10740

10681 ggcaactcgt ggcggcggcg ttccttacca cgtacgttcc tctaccgcgg gttgtcaggg 10740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10741 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 10800

10741 ggccggtgcc ccggacggtg gtatgggtgc ggctttgttc gcgagtactc gggcttcacc 10800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10801 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 10860

10801 gctcgggcta gaaggggtag ccactacagc cgctatatcc gcggtcgttg gcgtggacac 10860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 10920

10861 cgcggccact acggccggtg ctacgcaggc cgcatctcct agctctagag ctagggcgct 10920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10921 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 10980

10921 ttaattatgc tgagtgatat ccccttaaca ctcgcctatt gttaagggga gatctttatt 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 ttttgtttaa ctttaagaag gagatataca tatggctagc atgactggtg gacagcaaat 11040

10981 aaaacaaatt gaaattcttc ctctatatgt ataccgatcg tactgaccac ctgtcgttta 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11041 gggtcgcgga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg 11100

11041 cccagcgcct aggccgacga ttgtttcggg ctttccttcg actcaaccga cgacggtggc 11100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11101 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc 11160

11101 gactcgttat tgatcgtatt ggggaacccc ggagatttgc ccagaactcc ccaaaaaacg 11160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11161 tgaaaggagg aactatatcc ggatatcccg caagaggccc ggcagtaccg gcataaccaa 11220

11161 actttcctcc ttgatatagg cctatagggc gttctccggg ccgtcatggc cgtattggtt 11220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11221 gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga 11280

11221 cggatacgga tgtcgtaggt cccactgcca cggctcctac tgctactcgc gtaacaatct 11280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11281 tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag 11340

11281 aaagtatgtg ccacggactg acgcaatcgt taaattgaca ctatttgatg gcgtaatttc 11340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11341 cttatcgatg ataagctgtc aaacatgaga a......... .......... .......... 11371

11341 gaatagctac tattcgacag tttgtactct t......... .......... .......... 11371

.......... .......... .......... .......... .......... ..........