Sequence of pET-14b

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Sequence of pET-14b

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Sequence of pET-14b : 4671 BP; 1053 A; 1287 C; 1211 G; 1120 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... ......... tet(w/ gaps) .........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... NheI(229) ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ......... tet(w/ gaps) T7_term ......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... .. T7_term tet(w/ gaps) T7_Terminal_pri .. ..........

.......... .......... ..... BamHI(510),XhoI(515) ..... ..........

481 cagcttcctt tcgggctttg ttagcagccg gatcctcgag catatggctg ccgcgcggca 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctaggagctc gtataccgac ggcgcgccgt 540

......... T7_term tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta 600

541 ggtccggcga cgacactact actactacta ccgacgacgg gtaccatata gaggaagaat 600

.......... ......... tet(w/ gaps) ......... ....... T7_transl_en_RBS

.......... XbaI(619) .......... .......... .......... ..........

601 aagttaaaca aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat 660

601 ttcaatttgt tttaataaag atctcccttt ggcaacacca gagggatatc ac tcagcata 660

.......... ......... tet(w/ gaps) ......... .......... T7_pro

.......... BglII(677) .......... .......... .......... ..........

661 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc 720

661 attaaagcgc cctagctcta gagctaggag atgcggcctg cgtagcaccg gccgtagtgg 720

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 780

721 ccgcggtgtc cacgccaacg accgcggata tagcggctgt agtggctacc ccttctagcc 780

.......... ....... tet(w/ gaps) pBRrevBam_pri ....... ..........

.......... .......... .......... .......... .......... ..........

781 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 840

781 cgagcggtga agcccgagta ctcgcgaaca aagccgcacc cataccaccg tccggggcac 840

.......... ......... tet(w/ gaps) ORF 1 ......... ..........

.......... .......... .......... .......... .......... ..........

841 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 900

841 cggccccctg acaacccgcg gtagaggaac gtacgtggta aggaacgccg ccgccacgag 900

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... SalI(959)

901 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 960

901 ttgccggagt tggatgatga cccgacgaag gattacgtcc tcagcgtatt ccctctcgca 960

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 1020

961 gctggctacg ggaactctcg gaagttgggt cagtcgagga aggccacccg cgccccgtac 1020

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 1080

1021 tgatagcagc ggcgtgaata ctgacagaag aaatagtacg ttgagcatcc tgtccacggc 1080

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 1140

1081 cgtcgcgaga cccagtaaaa gccgctcctg gcgaaagcga cctcgcgctg ctactagccg 1140

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc 1200

1141 gacagcgaac gccataagcc ttagaacgtg cgggagcgag ttcggaagca gtgaccaggg 1200

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg 1260

1201 cggtggtttg caaagccgct cttcgtccgg taatagcggc cgtaccgccg gctgcgcgac 1260

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... NruI(1282) .......... .......... ..........

1261 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt 1320

1261 ccgatgcaga acgaccgcaa gcgctgcgct ccgacctacc ggaaggggta atactaagaa 1320

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat 1380

1321 gagcgaaggc cgccgtagcc ctacgggcgc aacgtccggt acgacaggtc cgtccatcta 1380

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc 1440

1381 ctgctggtag tccctgtcga agttcctagc gagcgccgag aatggtcgga ttgaagctag 1440

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg 1500

1441 tgacctggcg actagcagtg ccgctaaata cggcggagcc gctcgtgtac cttgcccaac 1500

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca 1560

1501 cgtacctaac atccgcggcg ggatatggaa cagacggagg ggcgcaacgc agcgccacgt 1560

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca 1620

1561 acctcggccc ggtggagctg gacttacctt cggccgccgt ggagcgattg cctaagtggt 1620

...... tet(w/ gaps) ORF 1 ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt 1680

1621 gaggttctta acctcggtta gttaagaacg cctcttgaca cttacgcgtt tggttgggaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca 1740

1681 ccgtcttgta taggtagcgc aggcggtaga ggtcgtcggc gtgcgccgcg tagagcccgt 1740

.......... .......... .......... .......... .......... ..........

.......... MscI(1754) .......... .......... .......... ..........

1741 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag 1800

1741 cgcaacccag gaccggtgcc cacgcgtact agcacgagga cagcaactcc tgggccgatc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa 1860

1801 cgaccgcccc aacggaatga ccaatcgtct tacttagtgg ctatgcgctc gcttgcactt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc 1920

1861 cgctgacgac gacgttttgc agacgctgga ctcgttgttg tacttaccag aagccaaagg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct 1980

1921 cacaaagcat ttcagacctt tgcgccttca gtcgcgggac gtggtaatac aaggcctaga 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct 2040

1981 cgtagcgtcc tacgacgacc gatgggacac cttgtggatg tagacataat tgcttcgcga 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac 2100

2041 ccgtaactgg gactcactaa aaagagacca gggcggcgta ggtatggcgg tcaacaaatg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc 2160

2101 ggagtgttgc aaggtcattg gcccgtacaa gtagtagtca ttgggcatag cactcgtagg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat 2220

2161 agagagcaaa gtagccatag taatgggggt acttgtcttt agggggaatg tgcctccgta 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat 2280

2221 gtcactggtt tgtccttttt tggcgggaat tgtaccgggc gaaatagtct tcggtctgta 2280

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2281 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat 2340

2281 attgcgaaga cctctttgag ttgctcgacc tgcgcctact tgtccgtctg tagacactta 2340

.......... .......... ROP .......... .......... ..........

.......... .......... ......... PvuII(2374) ......... ..........

2341 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg 2400

2341 gcgaagtgct ggtgcgacta ctcgaaatgg cgtcgacgga gcgcgcaaag ccactactgc 2400

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 2460

2401 cacttttgga gactgtgtac gtcgagggcc tctgccagtg tcgaacagac attcgcctac 2460

ROP pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 2520

2461 ggccctcgtc tgttcgggca gtcccgcgca gtcgcccaca accgcccaca gccccgcgtc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 2580

2521 ggtactgggt cagtgcatcg ctatcgcctc acatatgacc gaattgatac gccgtagtct 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg 2640

2581 cgtctaacat gactctcacg tggtatatac gccacacttt atggcgtgtc tacgcattcc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 2700

2641 tcttttatgg cgtagtccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 2760

2701 caagccgacg ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 2820

2761 agtcccctat tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 2880

2821 tttttccggc gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt 2880

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 2940

2881 ttagctgcga gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 3000

2941 gggggacctt cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 3060

3001 aggcggaaag agggaagccc ttcgcaccgc gaaagagtat cgagtgcgac atccatagag 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 3120

3061 tcaagccaca tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 3180

3121 ctggcgacgc ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 3240

3181 agcggtgacc gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 3300

3241 tgtctcaaga acttcaccac cggattgatg ccgatgtgat cttcctgtca taaaccatag 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 3360

3301 acgcgagacg acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 3420

3361 gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 3480

3421 tttcctagag ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt 3480

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 3540

3481 ttgagtgcaa ttccctaaaa ccagtactct aatagttttt cctagaagtg gatctaggaa 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 3600

3541 aatttaattt ttacttcaaa atttagttag atttcatata tactcatttg aaccagactg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 3660

3601 tcaatggtta cgaattagtc actccgtgga tagagtcgct agacagataa agcaagtagg 3660

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3661 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 3720

3661 tatcaacgga ctgaggggca gcacatctat tgatgctatg ccctcccgaa tggtagaccg 3720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 3780

3721 gggtcacgac gttactatgg cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat 3780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 3840

3781 ttggtcggtc ggccttcccg gctcgcgtct tcaccaggac gttgaaatag gcggaggtag 3840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 3900

3841 gtcagataat taacaacggc ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg 3900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... PstI(3921) .......... .......... ..........

3901 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 3960

3901 ttgcaacaac ggtaacgacg tccgtagcac cacagtgcga gcagcaaacc ataccgaagt 3960

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 4020

3961 aagtcgaggc caagggttgc tagttccgct caatgtacta gggggtacaa cacgtttttt 4020

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 4080

4021 cgccaatcga ggaagccagg aggctagcaa cagtcttcat tcaaccggcg tcacaatagt 4080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 4140

4081 gagtaccaat accgtcgtga cgtattaaga gaatgacagt acggtaggca ttctacgaaa 4140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 4200

4141 agacactgac cactcatgag ttggttcagt aagactctta tcacatacgc cgctggctca 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 4260

4201 acgagaacgg gccgcagttg tgccctatta tggcgcggtg tatcgtcttg aaattttcac 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 4320

4261 gagtagtaac cttttgcaag aagccccgct tttgagagtt cctagaatgg cgacaactct 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 4380

4321 aggtcaagct acattgggtg agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 4440

4381 tcgcaaagac ccactcgttt ttgtccttcc gttttacggc gttttttccc ttattcccgc 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 4500

4441 tgtgccttta caacttatga gtatgagaag gaaaaagtta taataacttc gtaaatagtc 4500

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 4560

4501 ccaataacag agtactcgcc tatgtataaa cttacataaa tctttttatt tgtttatccc 4560

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 4620

4561 caaggcgcgt gtaaaggggc ttttcacggt ggactgcaga ttctttggta ataatagtac 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRI(4669) .........

4621 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a......... 4671

4621 tgtaattgga tatttttatc cgcatagtgc tccgggaaag cagaagttct t......... 4671

.......... .......... .......... .......... .......... ..........